Tunable endogenous protein degradation with heterobifunctional compounds

ABSTRACT

The present invention provides a means to modulate gene expression in vivo in a manner that avoids problems associated with CRISPR endogenous protein knock-out or knock-in strategies and strategies that provide for correction, or alteration, of single nucleotides. The invention includes inserting into the genome a nucleotide encoding a heterobifunctional compound targeting protein (dTAG) in-frame with the nucleotide sequence of a gene encoding an endogenously expressed protein of interest which, upon expression, produces an endogenous protein-dTAG hybrid protein. This allows for targeted protein degradation of the dTAG and the fused endogenous protein using a heterobifunctional compound.

RELATED APPLICATIONS

This application claims the benefit of provisional U.S. PatentApplication No. 62/456,654, filed Feb. 8, 2017, and provisional U.S.Patent Application No. 62/457,127, filed Feb. 9, 2017. The entirety ofthese applications are hereby incorporated by reference for allpurposes.

INCORPORATION BY REFERENCE

The contents of the text file named“16010-025WO1_sequencelisting_ST25.txt” which was created on Jan. 26,2018, and is 287 kilobytes in size, are hereby incorporated by referencein their entirety.

FIELD OF THE INVENTION

This invention describes methods, compounds, and compositions tomodulate an endogenously expressed protein using targeted proteindegradation.

BACKGROUND

Many tools have been developed to manipulate gene expression tointerrogate the function of a gene or protein of interest. For example,techniques such as RNA interference and antisense deoxyoligonucleotidesare commonly used to disrupt protein expression at the RNA and DNAlevel. Homologous recombination or loss-of-function mutations can beaccomplished using site-specific double-strand breaks using zinc-fingernucleases, transcription activator-like effector nucleases (TALENs), orclustered regulatory interspaced short palindromic repeat (CRISPR)-Cas9(Cheng, J. K. and Alper, H. S., “The genome editing toolbox: a spectrumof approaches for targeted modification” Curr. Opin. Biotechnol., 30C,(2014): 87-94; and Graham et al., Gen Biol, (2015): 16:260). TheCRISPR-Cas9 system has been used to modulate endogenous gene expressionby incorporating specific mutations into a gene of interest (see, forexample, Lo et al., Genetics, 2013; 195(2): 331-348; Yu et al., BiologyOpen, 2014; 3:271-280; Park et al., PLOS One, 2013; 9(4):e95101; Lackneret al., Nature Communications, 2015; 17(6): 1-7; U.S. Pat. Nos.8,771,945 and 9,228,208; WO 2014/204729; and U.S. Publication2014/0273235).

For example, the CRISPR-Cas9 system was employed to mutate the humanPCSK9 gene in chimeric liver-humanized mice bearing human hepatocytes(Wang, X., et al. “CRISPR-Cas9 Targeting of PCSK9 in Human HepatocytesIn Vivo.” Arteriosclerosis, Thrombosis, and Vascular Biology, (2016).).PCSK9 was successfully mutated and the CRISPR-Cas9 system has beenproposed to be useful as a way to treat human disorders in vivo.However, the long-term implications of permanent genome modification areunknown and concerns exist over the imperfect precision of genomeediting, the continuous activity of virally-delivered CRISPR-Cas9, andthe impact of direct correction in adults where biological compensationmechanisms may exist (Kormann et al., “Expression of therapeuticproteins after delivery of chemically modified mRNA in mice” Nat.Biotechnol., 29, (2011):154-157; Cho et al., “Analysis of off-targeteffects of CRISPR/Cas-derived RNA-guided endonucleases and nickases.”Genome Res., 24, (2014):132-141). Furthermore, CRISPR knock-outstrategies may be undesirable where the protein expressed, even ifimperfect, is essential for cellular function.

Efforts have been made to modulate gene expression in vitro usinginducible degradation systems. For example, the auxin-inducibledegradation (AID) system in plants has enabled controlled proteindepletion in yeast and cultured vertebrate cells. This system relies onexpression of a plant-specific F-box protein, TIR1, which regulatesdiverse aspects of plant growth and morphogenesis in response to thephytohormone auxin. TIR1 is the substrate recognition component of aSkpl-Cullin-F-box E3 ubiquitin ligase complex, which recognizessubstrates only in the presence of auxin and targets them fordegradation by the proteasome. This system has been manipulated andshown to enable conditional auxin-dependent protein depletion inCaenorhabditis elegans as well as in human HCT116 cells (see, forexample, Zhang et al., Development, 2015; 142: 4374-4384 and Natsume etal., Cell Reports, 2016; 15: 210-218). However, this approach isimpractical as an in vivo modulation system due to the toxicity ofauxin.

An alternative approach to reversibly controlling gene expression hasbeen the use of ligand-dependent destabilization domains and theShield-1 ligand, which allows for reversible stabilization anddestabilization of a tagged protein of interest in a dose-dependentmanner (see, for example, Rakhit et al., Chemistry & Biology, 2014; 21:1238-1252). Fusing the destabilizing domain to a gene of interestresults in the expression a fused protein that is degraded by theproteasome. Shield-1 binds specifically to the destabilization domainand inactivates protein degradation. However, this system is also notviable as an in vivo modulation strategy due to the requirement for thepresence of Sield-1 in the cell cytoplasm in order to avoid degradation.Such an approach would require a constant administration of Shield-1 tomaintain protein stability.

Thus, there remains an unmet need for improved systems that allow forreversible control of endogenous gene expression in vivo while providingimproved treatment modalities in subjects suffering from disorders suchas proteopathies.

It is therefore an object of the present invention to provide methods,compounds, and compositions to modulate gene expression in vivo in amanner that avoids problems associated with CRISPR endogenous proteinknock-out or knock-in strategies.

SUMMARY OF THE INVENTION

The present invention provides a means to modulate gene expression invivo in a manner that avoids problems associated with CRISPR endogenousprotein knock-out or knock-in strategies and strategies that provide forcorrection, or alteration, of single nucleotides. The invention includesinserting into the genome a nucleotide encoding a heterobifunctionalcompound targeting protein (dTAG) in-frame with the nucleotide sequenceof a gene encoding an endogenously expressed protein of interest which,upon expression, produces an endogenous protein-dTAG hybrid protein.This allows for targeted protein degradation of the dTAG and the fusedendogenous protein using a heterobifunctional compound in a controlled,tunable fashion.

A heterobifunctional compound, as contemplated herein, is a compoundthat binds to an ubiquitin ligase through a ubiquitin ligase bindingmoiety and also binds to the dTAG through its dTAG Targeting Ligand invivo, as defined in more detail below. Heterobifunctional compounds arecapable of induced proteasome-mediated degradation of the fusedendogenous proteins via recruitment to E3 ubiquitin ligase andsubsequent ubiquitination. These drug-like molecules offer thepossibility of reversible, dose-responsive, tunable, temporal controlover protein levels.

Compared to CRISPR-Cas9 genome editing that incorporates irreversiblechanges into a gene of interest, the use of a heterobifunctionalcompound to target endogenously expressed proteins with a dTAG allowsfor reversible control of the endogenously expressed protein ofinterest. Accordingly, the heterobifunctional compound can be used as arheostat of protein expression affording the ability to turn endogenousprotein expression on and off upon titration of the heterobifunctionalcompound. Furthermore, by genomically and stably incorporating a nucleicacid sequence encoding a dTAG in frame, either 5′- or 3′- to the gene ofthe endogenous protein, side effects associated with CRISPR-Cas9 such asnegative downstream consequences associated with permanently editing agene can be avoided.

The invention provides a mechanism to control the degradation ofendogenous proteins that mediate a disease by combining genomeengineering with small molecule activation/modulation of degradation.The methods and compositions described herein are particularly usefulfor targeting endogenous proteins associated with disease due to a gainof function, toxic accumulation, overexpression, or downstream enzymaticprocess the protein may be involved in. Applications of this technologyinclude, but are not limited to 1) targeted degradation of proteinswhere pathology is a result of gain of function mutation(s), 2) targeteddegradation of proteins where pathology is a function of amplificationor increased expression, 3) targeted degradation of proteins that aremanifestations of monogenetic disease, 4) targeted degradation ofproteins where genetic predisposition manifests over longer periods andoften after alternative biological compensatory mechanisms are no longeradequate, for example, but not limited to, hypercholesterolemia andproteinopathies.

Therefore, in one embodiment, a method is provided that includes atleast the steps of:

-   -   (i) transforming relevant cells of a subject, typically a human,        with a nucleic acid sequence encoding a dTAG, wherein the        nucleic acid sequence is integrated genomically in-frame with a        nucleic acid sequence of an endogenous protein which is acting        as a mediator of disease, wherein insertion of the nucleic acid        encoding the dTAG into the genomic sequence results in an        endogenous protein-dTAG hybrid or fusion protein upon        expression; and    -   (ii) administering to the subject, as needed, a        heterobifunctional compound which binds to a) the inserted dTAG        and b) a ubiquitin ligase in a manner that brings the dTAG (and        thus the endogenous protein-dTAG hybrid protein) into proximity        of the ubiquitin ligase, such that the endogenous protein-dTAG        hybrid protein is ubiquitinated, and then degraded by the        proteasome.

In one embodiment, the subject's cell is transformed in vivo. In oneembodiment, the subject's cell is transformed ex vivo and administeredback to the subject. In one embodiment, the subject's cell is a livercell.

In one embodiment, a method is provided that includes the steps of:

administering to the subject, as needed, a heterobifunctional compound,wherein the subject has one or more cells which have been transformedwith a nucleic acid sequence encoding a dTAG, wherein the nucleic acidsequence is integrated genomically in-frame in a 5′ or 3′ orientationwith a nucleic acid sequence of an endogenous protein which is acting asa mediator of disease, wherein insertion of the nucleic acid encodingthe dTAG into the genomic sequence results in an endogenous protein-dTAGhybrid or fusion protein upon expression of the protein; and wherein theheterobifunctional compound binds to a) the inserted dTAG and b) aubiquitin ligase in a manner that brings the dTAG (and thus theendogenous protein-dTAG hybrid protein) into proximity of the ubiquitinligase, such that the endogenous protein-dTAG hybrid protein isubiquitinated, and then degraded by the proteasome.

As contemplated herein, the synthetic gene encoding the endogenousprotein of interest-dTAG hybrid is derived in vivo through the targetedinsertion of a nucleic acid encoding the dTAG in-frame either 5′- or 3′-to the nucleic acid encoding the protein of interest. This results in anin-frame gene fusion that is susceptible to proteasome mediateddegradation upon treatment with a heterobifunctional compound that iscapable of binding the dTAG. In a main embodiment, the dTAG does notsubstantially interfere with the function of the endogenously expressedprotein. In one embodiment, the dTAG is a non-endogenous peptide, whichallows for heterobifunctional compound selectivity and minimizes offtarget effects upon administration of the heterobifunctional compound.In one embodiment, the dTAG is an amino acid sequence derived from anendogenous protein which has been modified, for example through a “bump”strategy (see, for example, (see Clackson et al., “Redesigning anFKBP-ligand interface to generate chemical dimerizers with novelspecificity”, PNAS 95 (1998):10437-10442, incorporated herein byreference), so that the heterobifunctional compound binds only to orpreferentially to the modified amino acid sequence of the dTAG and notthe corresponding endogenously expressed protein.

Also contemplated herein is a method for the in vitro allele-specificregulation of an endogenous protein through the targeted insertion of anucleic acid sequence encoding a dTAG in frame either 5′- or 3′- to thegenomic sequence encoding a protein of interest, wherein insertion ofthe nucleic acid encoding the dTAG into the genomic sequence results inan endogenous protein-dTAG hybrid or fusion protein upon expression,wherein the endogenous protein-dTAG is capable of being degraded by aheterobifunctional compound which binds to a) the inserted dTAG and b) aubiquitin ligase in a manner that brings the dTAG (and thus theendogenous protein-dTAG hybrid protein) into proximity of a ubiquitinligase, such that the endogenous protein-dTAG hybrid protein isubiquitinated, and then degraded by the proteasome. By using the methodsdescribed herein to insert a nucleic acid encoding a dTAG in frame witha gene encoding an endogenous protein of interest, the expression of theresultant protein can be tightly controlled through the introduction ofa heterobifunctional compound capable of binding the dTAG, resulting inthe degradation of the endogenous protein. Importantly, by using aheterobifunctional compound, expression of the endogenous protein can bereversibly controlled, allowing for the examination of the effects ofprotein expression on the cell.

Accordingly, by regulating expression of endogenous proteins in thismanner, downstream effects of modulating protein expression can beexamined across a wide variety of proteins and cell types, and invarious physiological conditions. Because the heterobifunctionalcompound concentration within the cell can be titrated, protein-dTAGhybrid protein concentrations within the cell can be finely tuned,allowing for the conditional alteration of protein abundance within thecell and the ability to alter phenotype within the cell on demand. Inone embodiment, provided herein is a method of assessing proteinexpression attenuation in a cell comprising inserting a nucleic acidsequence encoding a dTAG in frame either 5′- or 3′- to a genomicsequence encoding a protein of interest, wherein insertion of thenucleic acid encoding the dTAG into the genomic sequence results in anendogenous protein-dTAG hybrid or fusion protein upon expression,wherein the endogenous protein-dTAG is capable of being degraded by aheterobifunctional compound which binds to a) the inserted dTAG and b) aubiquitin ligase in a manner that brings the dTAG (and thus theendogenous protein-dTAG hybrid protein) into proximity of a ubiquitinligase, such that the endogenous protein-dTAG hybrid protein isubiquitinated, and then degraded by the proteasome. In one embodiment,the heterobifunctional compound is administered to the cell so that theconcentration of the protein-dTAG hybrid protein in the cell ispartially degraded. In one embodiment, the heterobifunctional compoundis administered to the cell so that the concentration of the endogenousprotein-dTAG hybrid protein in the cell is completely degraded.

In one embodiment, provided herein is a method of identifying a proteintarget associated with a disease or disorder comprising inserting anucleic acid sequence encoding a dTAG in frame either 5′- or 3′- to thegenomic sequence encoding a protein of interest, wherein insertion ofthe nucleic acid encoding the dTAG into the genomic sequence results inan endogenous protein-dTAG hybrid or fusion protein upon expression,wherein the endogenous protein-dTAG is capable of being degraded by aheterobifunctional compound which binds to a) the inserted dTAG and b) aubiquitin ligase in a manner that brings the dTAG (and thus theendogenous protein-dTAG hybrid protein) into proximity of a ubiquitinligase, such that the endogenous protein-dTAG hybrid protein isubiquitinated, and then degraded by the proteasome, and measuring theeffects of protein degradation on the disorder or disease state of thecell. By using the methods described herein to insert nucleic acidsencoding dTAGs in frame with a gene encoding an endogenous protein ofinterest, downregulation of various proteins can be examined andpotential targets for treating disorders associated with a particulardisease state can be identified. In addition, the current methods can beutilized to validate a potential protein being targeted as associatedwith a disease state.

In a particular embodiment, the dTAGs for use in the present inventioninclude an amino acid sequence derived from an endogenous proteinkinase. In one embodiment, the endogenous protein kinase amino acidsequence includes a mutation rendering the kinase inactive. In oneembodiment, the mutation in the protein kinase occurs within a conservedkinase catalytic triad amino acid sequence. In one embodiment, theconserved kinase catalytic triad amino acid sequence is TVS. In oneembodiment, the conserved kinase catalytic triad amino acid sequence isHRD. In one embodiment, the conserved kinase catalytic triad amino acidsequence is DFG. In one embodiment, the conserved kinase catalytic triadamino acid sequence is TRD. See Kornev et al., “Surface comparison ofactive and inactive protein kinases identifies a conserved activationmechanism,” PNAS 2006; 103(47):17783-17788, incorporated herein byreference. In one embodiment, at least one of the catalytic triad aminoacids is substituted for an alanine. In one embodiment, at least one ofthe catalytic triad amino acids is substituted for a glycine. In oneembodiment, the heterobifunctional compound contains an allelic-specificligand capable of selectively binding the mutant protein kinasesequence. In one embodiment, the mutant kinase is as described inRoskoski et al., “Classification of small molecule protein kinaseinhibitors based upon the structures of their drug-enzyme complexes,”Pharmacological Research http://dx.doi.org/10.1016/j.phrs.2015.10.021,incorporated herein by reference and/or Roskoski et al., “A historicaloverview of protein kinases and their targeted small moleculeinhibitors,” Pharmaceutical Research (2015),http://dx.doi.org/10.1016/j.phrs.2015.07.10, incorporated herein byreference. In one embodiment, the dTAG is derived from a kinase that isan analog-sensitive kinase. In one embodiment, the mutant kinase is asdescribed in Zhang et al., “Structure-guided inhibitor design expandsthe scope of analog-sensitive kinase technology,” ACS Chem Biol.2013:8(9); 1931-1938, incorporated herein by reference. In alternativeembodiments, the dTAGs for use in the present invention include, but arenot limited to, amino acid sequences derived from proteins selected fromEGFR, BCR-ABL, ALK, JAK2, BRAF, LRRK2, PDGFRα, and RET. In oneembodiment, the proteins contain one or more mutations. In oneembodiment, the one or more mutations render the protein inactive.

In alternative embodiments, the dTAGs for use in the present inventioninclude, but are not limited to, amino acid sequences derived fromproteins selected from Src, Src, Pkd1, Kit, Jak2, Abl, Mek1, HIVintegrase, and HIV reverse transcriptase.

In particular embodiments, the dTAGs for use in the present inventioninclude, but are not limited to, amino acid sequences derived fromendogenously expressed proteins such as FK506 binding protein-12(FKBP12), bromodomain-containing protein 4 (BRD4), CREB binding protein(CREBBP), or transcriptional activator BRG1 (SMARCA4). In otherembodiments, dTAGs for use in the present invention may include, forexample, a hormone receptor e.g. estrogen-receptor protein, androgenreceptor protein, retinoid x receptor (RXR) protein, or dihydrofolatereductase (DHFR), including bacterial DHFR. In other embodiments, thedTAG may include, for example, an amino acid sequence derived from abacterial dehalogenase. In other embodiments, the dTAG, may include,amino acid sequences derived from 7,8-dihydro-8-oxoguanintriphosphatase, AFAD, Arachidonate 5-lipoxygenase activating protein,apolipoprotein, ASH1L, ATAD2, baculoviral IAP repeat-containing protein2, BAZ1A, BAZ1B, BAZ2A, BAZ2B, Bcl-2, Bcl-xL, BRD1, BRD2, BRD3, BRD4,BRD5, BRD6, BRD7, BRD8, BRD9, BRD10, BRDT, BRPF1, BRPF3, BRWD3, CD209,CECR2, CREBBP, E3 ligase XIAP, EP300, FALZ, fatty acid binding proteinfrom adipocytes 4 (FABP4), GCN5L2, GTPase k-RAS, HDAC6, hematopoieticprostaglandin D synthase, KIAA1240, lactoglutathione lyase, LOC93349,Mcl-1, MLL, PA2GA, PB1, PCAF, peptidyl-prolyl cis-trans isomeraseNIMA-interacting 1, PHIP, poly-ADP-ribose polymerase 14, poly-ADP-ribosepolymerase 15, PRKCBP1, prosaposin, prostaglandin E synthase, retinalrod rhodopsin-sensitive cGMP 3′,′5-cyclic phosphodiesterase subunitdelta, S100-A7, SMARCA2, SMARCA4, SP100, SP110, SP140, Src,Sumo-conjugating enzyme UBC9, superoxide dismutase, TAF1, TAF1L,tankyrase 1, tankyrase 2, TIFla, TRIM28, TRIM33, TRIM66, WDR9, ZMYND11,or MLL4. In yet further embodiments, the dTAG may include, for example,an amino acid sequence derived from MDM2.

In a particular embodiment, the dTAG is derived from BRD2, BRD3, BRD4,or BRDT. In certain embodiments, the dTAG is a modified or mutant BRD2,BRD3, BRD4, or BRDT protein. In certain embodiments, the one or moremutations of BRD2 include a mutation of the Tryptophan (W) at amino acidposition 97, a mutation of the Valine (V) at amino acid position 103, amutation of the Leucine (L) at amino acid position 110, a mutation ofthe W at amino acid position 370, a mutation of the V at amino acidposition 376, or a mutation of the L at amino acid position 381.

In certain embodiments, the one or more mutations of BRD3 include amutation of the W at amino acid position 57, a mutation of the V atamino acid position 63, a mutation of the L at amino acid position 70, amutation of the W at amino acid position 332, a mutation of the V atamino acid position 338, or a mutation of the L at amino acid position345. In certain embodiments, the one or more mutations of BRD4 include amutation of the W at amino acid position 81, a mutation of the V atamino acid position 87, a mutation of the L at amino acid position 94, amutation of the W at amino acid position 374, a mutation of the V atamino acid position 380, or a mutation of the L at amino acid position387. In certain embodiments, the one or more mutations of BRDT include amutation of the W at amino acid position 50, a mutation of the V atamino acid position 56, a mutation of the L at amino acid position 63, amutation of the W at amino acid position 293, a mutation of the V atamino acid position 299, or a mutation of the L at amino acid position306.

In a particular embodiment, the dTAG is derived from cytosolic signalingprotein FKBP12. In certain embodiments, the dTAG is a modified or mutantcytosolic signaling protein FKBP12. In certain embodiments, the modifiedor mutant cytosolic signaling protein FKBP12 contains one or moremutations that create an enlarged binding pocket for FKBP12 ligands. Incertain embodiments, the one or more mutations include a mutation of thephenylalanine (F) at amino acid position 36 to valine (V) (F36V)(referred to interchangeably herein as FKBP* or FKBP12*).

In one embodiment, the dTAG is derived from an amino acid sequence, orfragment thereof from any of SEQ. ID. NOs.: 1-44. In a particularembodiment, the dTAG is derived from an amino acid sequence, or fragmentthereof of SEQ. ID. NO.: 1. In a particular embodiment, the dTAG isderived from an amino acid sequence, or fragment thereof of SEQ. ID.NO.: 2. In a particular embodiment, the dTAG is derived from an aminoacid sequence, or fragment thereof of SEQ. ID. NO.: 3. In a particularembodiment, the dTAG is derived from an amino acid sequence, or fragmentthereof of SEQ. ID. NO.: 4. In a particular embodiment, the dTAG isderived from an amino acid sequence, or fragment thereof of SEQ. ID.NO.: 5. In a particular embodiment, the dTAG is derived from an aminoacid sequence, or fragment thereof of SEQ. ID. NO.: 6. In a particularembodiment, the dTAG is derived from an amino acid sequence, or fragmentthereof of SEQ. ID. NO.: 7. In a particular embodiment, the dTAG isderived from an amino acid sequence, or fragment thereof of SEQ. ID.NO.: 8. In a particular embodiment, the dTAG is derived from an aminoacid sequence, or fragment thereof of SEQ. ID. NO.: 9. In a particularembodiment, the dTAG is derived from an amino acid sequence, or fragmentthereof of SEQ. ID. NO.: 10. In a particular embodiment, the dTAG isderived from an amino acid sequence, or fragment thereof of SEQ. ID.NO.: 11. In a particular embodiment, the dTAG is derived from an aminoacid sequence, or fragment thereof of SEQ. ID. NO.: 12. In a particularembodiment, the dTAG is derived from an amino acid sequence, or fragmentthereof of SEQ. ID. NO.: 13. In a particular embodiment, the dTAG isderived from an amino acid sequence, or fragment thereof of SEQ. ID.NO.: 14. In a particular embodiment, the dTAG is derived from an aminoacid sequence, or fragment thereof of SEQ. ID. NO.: 15. In a particularembodiment, the dTAG is derived from an amino acid sequence, or fragmentthereof of SEQ. ID. NO.: 16. In a particular embodiment, the dTAG isderived from an amino acid sequence, or fragment thereof of SEQ. ID.NO.: 17. In a particular embodiment, the dTAG is derived from an aminoacid sequence, or fragment thereof of SEQ. ID. NO.: 18. In a particularembodiment, the dTAG is derived from an amino acid sequence, or fragmentthereof of SEQ. ID. NO.: 19. In a particular embodiment, the dTAG isderived from an amino acid sequence, or fragment thereof of SEQ. ID.NO.: 20. In a particular embodiment, the dTAG is derived from an aminoacid sequence, or fragment thereof of SEQ. ID. NO.: 21. In a particularembodiment, the dTAG is derived from an amino acid sequence, or fragmentthereof of SEQ. ID. NO.: 22. In a particular embodiment, the dTAG isderived from an amino acid sequence, or fragment thereof of SEQ. ID.NO.: 23. In a particular embodiment, the dTAG is derived from an aminoacid sequence, or fragment thereof of SEQ. ID. NO.: 24. In a particularembodiment, the dTAG is derived from an amino acid sequence, or fragmentthereof of SEQ. ID. NO.: 25. In a particular embodiment, the dTAG isderived from an amino acid sequence, or fragment thereof of SEQ. ID.NO.: 26. In a particular embodiment, the dTAG is derived from an aminoacid sequence, or fragment thereof of SEQ. ID. NO.: 27. In a particularembodiment, the dTAG is derived from an amino acid sequence, or fragmentthereof of SEQ. ID. NO.: 28. In a particular embodiment, the dTAG isderived from an amino acid sequence, or fragment thereof of SEQ. ID.NO.: 29. In a particular embodiment, the dTAG is derived from an aminoacid sequence, or fragment thereof of SEQ. ID. NO.: 30. In a particularembodiment, the dTAG is derived from an amino acid sequence, or fragmentthereof of SEQ. ID. NO.: 31. In a particular embodiment, the dTAG isderived from an amino acid sequence, or fragment thereof of SEQ. ID.NO.: 32. In a particular embodiment, the dTAG is derived from an aminoacid sequence, or fragment thereof of SEQ. ID. NO.: 33. In a particularembodiment, the dTAG is derived from an amino acid sequence, or fragmentthereof of SEQ. ID. NO.: 34. In a particular embodiment, the dTAG isderived from an amino acid sequence, or fragment thereof of SEQ. ID.NO.: 35. In a particular embodiment, the dTAG is derived from an aminoacid sequence, or fragment thereof of SEQ. ID. NO.: 36. In a particularembodiment, the dTAG is derived from an amino acid sequence, or fragmentthereof of SEQ. ID. NO.: 37. In a particular embodiment, the dTAG isderived from an amino acid sequence, or fragment thereof of SEQ. ID.NO.: 38. In a particular embodiment, the dTAG is derived from an aminoacid sequence, or fragment thereof of SEQ. ID. NO.: 39. In a particularembodiment, the dTAG is derived from an amino acid sequence, or fragmentthereof of SEQ. ID. NO.: 40. In a particular embodiment, the dTAG isderived from an amino acid sequence, or fragment thereof of SEQ. ID.NO.: 41. In a particular embodiment, the dTAG is derived from an aminoacid sequence, or fragment thereof of SEQ. ID. NO.: 42. In a particularembodiment, the dTAG is derived from an amino acid sequence, or fragmentthereof of SEQ. ID. NO.: 43. In a particular embodiment, the dTAG isderived from an amino acid sequence, or fragment thereof of SEQ. ID.NO.: 44. In a particular embodiment, the fragment thereof refers to theminimum amino acid sequence need to be bound by the heterobifunctionalcompound. In a particular embodiment, the dTAG is derived from an aminoacid sequence, or fragment thereof of SEQ. ID. NO.: 62. In a particularembodiment, the dTAG is derived from an amino acid sequence, or fragmentthereof of SEQ. ID. NO.: 63. In a particular embodiment, the fragmentthereof refers to the minimum amino acid sequence needed to be bound bythe heterobifunctional compound.

In a particular embodiment, the dTAG is derived from an amino acidsequence or fragment thereof of SEQ. ID. NO.: 1 and the dTAG is capableof being bound by a heterobifunctional compound selected from any ofdFKBP-1-dFKBP-5. In a particular embodiment, the dTAG is derived from anamino acid sequence or fragment thereof of SEQ. ID. NO.: 2 and the dTAGis capable of being bound by a heterobifunctional compound selected fromany of dFKBP-6-dFKBP-13. In a particular embodiment, the dTAG is derivedfrom an amino acid sequence or fragment thereof of SEQ. ID. NO.: 3 andthe dTAG is capable of being bound by a heterobifunctional compoundselected from any of dBET1-dBET18. In a particular embodiment, the dTAGis derived from an amino acid sequence or fragment thereof of SEQ. ID.NO.: 3 and the dTAG is capable of being bound by a heterobifunctionalcompound selected from any of dBromo1-dBromo34. In a particularembodiment, the dTAG is derived from an amino acid sequence or fragmentthereof of SEQ. ID. NO.: 9 and the dTAG is capable of being bound by aheterobifunctional compound selected from any of dHalo1-dHalo2.

In one embodiment, the dTAG is derived from any amino acid sequencedescribed herein, or a fragment thereof, and the dTAG is capable ofbeing bound by a corresponding heterobifunctional compound comprising adTAG Targeting Ligand capable of binding the dTAG described herein. Inone embodiment, the dTAG is amino acid sequence capable of being boundby a heterobifunctional compound described in FIG. 29, FIG. 30, FIG. 31,FIG. 32, and FIG. 33, or any other heterobifunctional compound describedherein. In one embodiment, the dTAG is amino acid sequence capable ofbeing bound by a heterobifunctional compound comprising a dTAG TargetingLigand described in Table T. In a particular embodiment, the dTAG isderived from an amino acid sequence or fragment thereof of SEQ. ID. NO.:1 and the dTAG is capable of being bound by a heterobifunctionalcompound selected from any of dFKBP-1-dFKBP-5. In a particularembodiment, the dTAG is derived from an amino acid sequence or fragmentthereof of SEQ. ID. NO.: 2 and the dTAG is capable of being bound by aheterobifunctional compound selected from any of dFKBP-6-dFKBP-13. In aparticular embodiment, the dTAG is derived from an amino acid sequenceor fragment thereof of SEQ. ID. NO.: 3 and the dTAG is capable of beingbound by a heterobifunctional compound selected from any ofdBET1-dBET18.

In a particular embodiment, the dTAG is derived from an amino acidsequence or fragment thereof of SEQ. ID. NO.: 3 and the dTAG is capableof being bound by a heterobifunctional compound selected from any ofdBromo1-dBromo34. In a particular embodiment, the dTAG is derived froman amino acid sequence or fragment thereof of SEQ. ID. NO.: 9 and thedTAG is capable of being bound by a heterobifunctional compound selectedfrom any of dHalo1-dHalo2. In a particular embodiment, the dTAG isderived from CREBBP and the heterobifunctional compound contains aCREBBP dTAG Targeting Ligand selected from Table T. In a particularembodiment, the dTAG is derived from SMARCA4, PB1, or SMARCA2 and theheterobifunctional compound contains a SMARCA4/PB1/SMARCA2 dTAGTargeting Ligand selected from Table T. In a particular embodiment, thedTAG is derived from TRIM24 or BRPF1 and the heterobifunctional compoundcontains a TRIM24/BRPF1 dTAG Targeting Ligand selected from Table T. Ina particular embodiment, the dTAG is derived from a glucocorticoidreceptor and the heterobifunctional compound contains a glucocorticoiddTAG Targeting Ligand selected from Table T. In a particular embodiment,the dTAG is derived from an estrogen or androgen receptor and theheterobifunctional compound contains an estrogen/androgen receptor dTAGTargeting Ligand selected from Table T. In a particular embodiment, thedTAG is derived from DOT1L and the heterobifunctional compound containsa DOT1L dTAG Targeting Ligand selected from Table T. In a particularembodiment, the dTAG is derived from Ras and the heterobifunctionalcompound contains a Ras dTAG Targeting Ligand selected from Table T. Ina particular embodiment, the dTAG is derived from RasG12C and theheterobifunctional compound contains a RasG12C dTAG Targeting Ligandselected from Table T. In a particular embodiment, the dTAG is derivedfrom HER3 and the heterobifunctional compound contains a HER3 dTAGTargeting Ligand selected from Table T. In a particular embodiment, thedTAG is derived from Bcl-2 or Bcl-XL and the heterobifunctional compoundcontains a Bcl-2/Bcl-XL dTAG Targeting Ligand selected from Table T. Ina particular embodiment, the dTAG is derived from HDAC and theheterobifunctional compound contains a HDAC dTAG Targeting Ligandselected from Table T. In a particular embodiment, the dTAG is derivedfrom PPAR and the heterobifunctional compound contains a PPAR dTAGTargeting Ligand selected from Table T. In a particular embodiment, thedTAG is derived from DHFR and the heterobifunctional compound contains aDHFR dTAG Targeting Ligand selected from Table T.

In one aspect, the synthetic gene of the present invention includes agene of interest that is implicated in a genetic disorder. By way of anon-limiting example, a mutated gene, for example, encoding alpha-1antitrypsin (A1AT), may be targeted for dTAG in frame insertion in acell to produce a synthetic gene which encodes a hybrid protein capableof being degraded by a heterobifunctional compound that targets the dTAGof the endogenous A1AT-dTAG hybrid protein. By generating an A1AT-dTAGhybrid, the function of the mutated A1AT can be regulated or modulatedthrough heterobifunctional compound administration, allowing the cell tomaintain some function of the A1AT endogenous protein while reducing theeffects of A1AT overexpression. Other non-limiting examples of proteinsthat may be targeted include β-catenin (CTNNB1), apolipoprotein B(APOB), angiopoietin-like protein 3 (ANGPTL3), proprotein convertasesubtilisin/kexin type 9 (PCSK9), apolipoprotein C3 (APOC3), low densitylipoprotein receptor (LDLR), C-reactive protein (CRP), apolipoprotein a(Apo(a)), Factor VII, Factor XI, antithrombin III (SERPINC1),phosphatidylinositol glycan class A (PIG-A), C5, alpha-1 antitrypsin(SERPINA1), hepcidin regulation (TMPRSS6), (delta-aminolevulinatesynthase 1 (ALAS-1), acylCaA:diacylglycerol acyltransferase (DGAT),miR-122, miR-21, miR-155, miR-34a, prekallikrein (KLKB1), connectivetissue growth factor (CCN2), intercellular adhesion molecule 1 (ICAM-1),glucagon receptor (GCGR), glucorticoid receptor (GCCR), protein tyrosinephosphatase (PTP-1B), c-Raf kinase (RAF1), fibroblast growth factorreceptor 4 (FGFR4), vascular adhesion molecule-1 (VCAM-1), very lateantigen-4 (VLA-4), transthyretin (TTR), survival motor neuron 2 (SMN2),growth hormone receptor (GHR), dystophia myotonic protein kinase (DMPK),cellular nucleic acid-binding protein (CNBP or ZNF9), clusterin (CLU),eukaryotic translation initiation factor 4E (eIF-4e), MDM2, MDM4, heatshock protein 27 (HSP 27), signal transduction and activator oftranscription 3 protein (STAT3), vascular endothelial growth factor(VEGF), kinesin spindle protein (KIF 11), hepatitis B genome, theandrogen receptor (AR), Atonal homolog 1 (ATOH1), vascular endothelialgrowth factor receptor 1 (FLT1), retinoschism 1 (RS1), retinal pigmentepithelium-specific 65 kDa protein (RPE65), Rab escort protein 1 (CHM),and the sodium channel, voltage gated, type X, alpha subunit (PN3 orSCN10A).

The genetic disorders include but are not limited to homozygous familialhypercholesterolemia, AGS1-AGS7, PRAAS/CANDLE, SAVI, ISG15 def, SPENCDI,hemophagocytic lymphohistiocytosis, NLRC4-MAS, CAMPS, DADA2, PLAID,Tyrosinemia type I, BSEP deficiency, MRD3 gene defect, glycogen storagedisease types IV, I, Crigler-Najjar syndrome, Ornithine transcarbamylasedeficiency, primary hyperoxaluria, Wilson disease, Cystic fibrosis, FIC1deficiency, citrullinemia, cystinosis, propionic academia, ADA-SCID,X-linked SCID, lipoprotein lipase deficiency, Leber's congenitalamaurosis, and adrenoleukodystrophy.

Also contemplated herein is the use of heterobifunctional compoundscapable of binding to the dTAG of the endogenous protein-dTAG hybrid ofthe present invention and inducing degradation through ubiquination. Byadministering to a subject a heterobifunctional compound directed to adTAG, the endogenous protein-dTAG hybrid can be modulated in a subjectsuffering from a disease or disorder as a result of the target protein'sexpression. The heterobifunctional compounds for use in the presentinvention are small molecule antagonists capable of disabling thebiological function of the endogenous protein through degradation of theendogenous protein-dTAG hybrid. They provide prompt ligand-dependenttarget protein degradation via chemical conjugation with, for example,derivatized phthalimides that hijack the function of the Cereblon E3ubiquitin ligase complex. Using this approach, the endogenousprotein-dTAG hybrid of the present invention can be degraded rapidlywith a high specificity and efficiency.

The heterobifunctional compounds that can be used in the presentinvention include those that include a small molecule E3 ligase ligandwhich is covalently linked to a dTAG Targeting Ligand through a Linkerof varying length and/or functionality as described in more detailbelow. The heterobifunctional compound is able to bind to the dTAG andrecruit an E3 ligase, for example, by binding to a Cereblon (CRBN)containing ligase or Von Hippel-Lindau tumor suppressor (VHL) to theendogenous-dTAG hybrid for ubiquitination and subsequent proteasomaldegradation.

Moreover, by combining the chemical strategy of protein degradation viathe bifunctional molecules of the present application with theeffectiveness of gene therapy, the activity of the endogenouslyexpressed protein, and thus the side effects, can be regulated in aprecise, temporal manner by rapidly turning on and off ubiquitination,and proteasomal degradation of the endogenous protein-dTAG hybrid.

Examples of heterobifunctional compounds useful in the present inventionare exemplified further below.

In one aspect, the genomic nucleic acid sequence encodes a syntheticgene comprising an endogenous gene of interest having a 5′- or3′-in-frame insertion of a nucleic acid encoding a dTAG which, whenexpressed, results in an endogenous protein-dTAG hybrid protein whereinthe dTAG is capable of being bound by a heterobifunctional compound.Cells and animals, including in particular non-human animals, bearingsuch genetic modifications are part of the invention.

In a particular embodiment, the genomic nucleic acid sequence encodes asynthetic gene comprising an endogenous gene of interest having a 5′- or3′-in-frame insertion of a nucleic acid encoding a dTAG wherein the dTAGis derived from an amino acid sequence or fragment thereof of SEQ. ID.NO.: 1 and the dTAG is capable of being bound by a heterobifunctionalcompound selected from any of dFKBP-1-dFKBP-5. In a particularembodiment, the genomic nucleic acid sequence encodes a synthetic genecomprising an endogenous gene of interest having a 5′- or 3′-in-frameinsertion of a nucleic acid encoding a dTAG wherein the dTAG is derivedfrom an amino acid sequence or fragment thereof of SEQ. ID. NO.: 2 andthe dTAG is capable of being bound by a heterobifunctional compoundselected from any of dFKBP-6-dFKBP-13. In a particular embodiment, thegenomic nucleic acid sequence encodes a synthetic gene comprising anendogenous gene of interest having a 5′- or 3′-in-frame insertion of anucleic acid encoding a dTAG wherein the dTAG is derived from an aminoacid sequence or fragment thereof of SEQ. ID. NO.: 3 and the dTAG iscapable of being bound by a heterobifunctional compound selected fromany of dBET1-dBET18. In a particular embodiment, the genomic nucleicacid sequence encodes a synthetic gene comprising an endogenous gene ofinterest having a 5′- or 3′-in-frame insertion of a nucleic acidencoding a dTAG wherein the dTAG is derived from an amino acid sequenceor fragment thereof of SEQ. ID. NO.: 3 and the dTAG is capable of beingbound by a heterobifunctional compound selected from any ofdBromo1-dBromo34. In a particular embodiment, the genomic nucleic acidsequence encodes a synthetic gene comprising an endogenous gene ofinterest having a 5′- or 3′-in-frame insertion of a nucleic acidencoding a dTAG wherein the dTAG is derived from an amino acid sequenceor fragment thereof of SEQ. ID. NO.: 9 and the dTAG is capable of beingbound by a heterobifunctional compound selected from dHalo1 and dHalo2.

In one aspect, an amino acid encoded by a synthetic gene comprising anendogenous gene of interest having a 5′- or 3′-in-frame insertion of anucleic acid encoding a dTAG is provided, wherein the amino acid beingan endogenous protein-dTAG hybrid protein wherein the dTAG is capable ofbeing bound by a heterobifunctional compound.

In one aspect, provided herein is a transformed cell comprising agenomic nucleic acid sequence encoding a synthetic gene comprising anendogenous gene of interest having a 5′- or 3′-in-frame insertion of anucleic acid encoding a dTAG which, when expressed, results in anendogenous protein-dTAG hybrid protein wherein the dTAG is capable ofbeing bound by a heterobifunctional compound.

In one aspect, provided herein is a cell expressing a synthetic genecomprising an endogenous gene of interest having a 5′- or 3′-in-frameinsertion of a nucleic acid encoding a dTAG which, when expressed,results in an endogenous protein-dTAG hybrid protein wherein the dTAG iscapable of being bound by a heterobifunctional compound.

In a particular aspect, a method of modulating the activity of anendogenous protein by genomically inserting in frame a nucleic acidsequence encoding a dTAG is provided which, when expressed, results inan endogenous protein-dTAG hybrid protein wherein the dTAG is capable ofbeing bound by a heterobifunctional compound, and administering to asubject a heterobifunctional compound capable of binding the dTAG anddegrading the endogenous protein-dTAG hybrid.

In a particular aspect, a method of identifying an endogenous proteinassociated with a disease state is provided wherein the activity of theendogenous protein is modulated by genomically inserting in frame anucleic acid sequence encoding a dTAG which, when expressed, results inan endogenous protein-dTAG hybrid protein wherein the dTAG is capable ofbeing bound by a heterobifunctional compound, and administering aheterobifunctional compound capable of binding the dTAG and degradingthe endogenous protein-dTAG hybrid, wherein degradation of the proteinresults in the alteration of the disease state.

In one embodiment, provided herein is a transformed cell comprising anucleic acid encoding SEQ. ID. NO.: 52 and a nucleic acid encoding adTAG. In one embodiment, provided herein is a transformed cellcomprising a nucleic acid encoding SEQ. ID. NO.: 52 and a nucleic acidencoding dTAG derived from an amino acid sequence, or fragment thereof,selected from SEQ. ID. NOs.: 1-44.

In one embodiment, provided herein is a first nucleic acid encoding SEQ.ID. NO.: 52 and a second nucleic acid encoding a dTAG. In oneembodiment, provided herein is a first nucleic acid encoding SEQ. ID.NO.: 52 and a second nucleic acid encoding a dTAG derived from an aminoacid sequence, or fragment thereof, selected from SEQ. ID. NO.: 1-44.

Other aspects of the invention include polynucleotide sequences,plasmids, and vectors encoding the synthetic genes of the presentinvention, and host cells expressing the synthetic genes of the presentinvention.

In an alternative embodiment, the dTAG for use in the present inventionis an amino acid sequence derived from EGFR. In certain embodiments, thedTAG is a modified or mutant EGFR protein or fragment thereof. Incertain embodiments, the one or more mutations of EGFR include asubstitution of Leucine (L) with Arginine (R) at amino acid position858, a deletion of the amino acid sequence LREA in exon 19, an insertionof amino acids VAIKEL in exon 19, a substitution of Glycine (G) withAlanine (A), Cysteine (C), or Serine (S) at amino acid position 719, asubstitution of Leucine (L) with Alanine (A), Cysteine (C), or Serine(S) at amino acid position 861, a substitution of Valine (V) withAlanine (A) at amino acid position 765, a substitution of Threonine (T)with Alanine (A) at amino acid position 783, a substitution of Serine(S) with Proline (P) at amino acid position 784, a substitution ofThreonine (T) with Methionine (M) at amino acid position 790 M, asubstitution of Threonine (T) with Alanine (A) at amino acid position854, a substitution of Aspartic Acid (D) with Tyrosine (Y) at amino acid761, a substitution of Leucine (L) with Serine (S) at amino acidposition 747, a substitution of Cysteine (C) with Serine (S) or Glycine(G) at amino acid position 797. In one embodiment, the dTAG is an aminoacid sequence derived from, or a fragment thereof, of SEQ. ID. NO.: 53.In one embodiment, the dTAG is an amino acid sequence derived from, or afragment thereof, of SEQ. ID. NO.: 54. In one embodiment, SEQ. ID. NO.:54 has a Leucine at position 163. In one embodiment, the dTAG is anamino acid sequence derived from, or a fragment thereof, of SEQ. ID.NO.: 55. In one embodiment, SEQ. ID. NO.: 55 has a Leucine at position163. In one embodiment, SEQ. ID. No.: 55 has a Threonine at position 95.In one embodiment, SEQ. ID. NO.: 55 has a Leucine at position 163 and aThreonine at position 95. In one embodiment, the dTAG is an amino acidsequence derived from, or a fragment thereof, of SEQ. ID. NO.: 56. Inone embodiment, SEQ. ID. NO.: 56 has a Leucine at position 163. In oneembodiment, SEQ. ID. NO.: 56 has a Threonine at position 95. In oneembodiment, SEQ. ID. NO.: 56 has a Leucine at position 163 and aThreonine at position 95.

In an alternative embodiment, the dTAG for use in the present inventionis an amino acid sequence derived from BCR-ABL. In certain embodiments,the dTAG is a modified or mutant BCR-ABL protein or fragment thereof. Incertain embodiments, the one or more mutations of BCR-ABL include asubstitution of Tyrosine (T) with Isoleucine (I) at amino acid position315. In one embodiment, the dTAG is an amino acid sequence derived from,or a fragment thereof, of SEQ. ID. NO.: 57. In one embodiment, the dTAGis an amino acid sequence derived from, or a fragment thereof, of SEQ.ID. NO.: 58.

In an alternative embodiment, the dTAGs for use in the present inventionis an amino acid sequence derived from ALK. In certain embodiments, thedTAG is a modified or mutant ALK protein or fragment thereof. In certainembodiments, the one or more mutations of ALK include a substitution ofLeucine (L) with Methionine at amino acid position 1196. In oneembodiment, the dTAG is an amino acid sequence derived from, or afragment thereof, of SEQ. ID. NO.: 59.

In an alternative embodiment, the dTAGs for use in the present inventionis an amino acid sequence derived from JAK2. In certain embodiments, thedTAG is a modified or mutant JAK2 protein or fragment thereof. Incertain embodiments, the one or more mutations of JAK2 include asubstitution of Valine (V) with Phenylalanine (F) at amino acid position617. In one embodiment, the dTAG is an amino acid sequence derived from,or a fragment thereof, of SEQ. ID. NO.: 60.

In an alternative embodiment, the dTAGs for use in the present inventionis an amino acid sequence derived from BRAF. In certain embodiments, thedTAG is a modified or mutant BRAF protein or fragment thereof. Incertain embodiments, the one or more mutations of BRAF include asubstitution of Valine (V) with Glutamic Acid (E) at amino acid position600. In one embodiment, the dTAG is an amino acid sequence derived from,or a fragment thereof, of SEQ. ID. NO.: 61.

In alternative embodiments, the dTAGs for use in the present inventioninclude, but are not limited to, amino acid sequences derived fromproteins selected from EGFR, BCR-ABL, ALK, JAK2, and BRAF. In oneembodiment, the proteins contain one or more mutations. In oneembodiment, the one or more mutations render the protein inactive.

In an alternative embodiment, the dTAG for use in the present inventionis an amino acid sequence derived from Src. In certain embodiments, thedTAG is a modified or mutant Src protein or fragment thereof. In certainembodiments, the one or more mutations or modifications of Src include asubstitution of Threonine (T) with Glycine (G) or Alanine (A) at aminoacid position 341. In one embodiment, the dTAG is an amino acid sequencederived from, or a fragment thereof, of SEQ. ID. NO.: 62. In oneembodiment, the dTAG is an amino acid sequence derived from, or afragment thereof, of SEQ. ID. NO.: 63.

In an alternative embodiment, the dTAG for use in the present inventionis an amino acid sequence derived from LKKR2. In certain embodiments,the dTAG is a modified or mutant LKKR2 protein or fragment thereof. Incertain embodiments, the one or more mutations of LKKR2 include asubstitution of Arginine (R) with Cysteine (C) at amino acid 1441, asubstitution of Glycine (G) with Serine (S) at amino acid 2019, asubstitution of Isoleucine (I) with Threonine (T) at amino acid 2020.

In an alternative embodiment, the dTAG for use in the present inventionis an amino acid sequence derived from PDGFRα. In certain embodiments,the dTAG is a modified or mutant PDGFRa protein or fragment thereof. Incertain embodiments, the one or more mutations of PDGFRa include asubstitution of Threonine (T) with Isoleucine (I) at amino acid 674.

In an alternative embodiment, the dTAG for use in the present inventionis an amino acid sequence derived from RET. In certain embodiments, thedTAG is a modified or mutant RET protein or fragment thereof. In certainembodiments, the one or more mutations of RET include a substitution ofGlycine (G) with Serine (S) at amino acid 691. In certain embodiments,the one or more mutations of RET include a substitution of Arginine (R)with Threonine (T) at amino acid 749. In certain embodiments, the one ormore mutations of RET include a substitution of Glutamic acid (E) withGlutamine (Q) at amino acid 762. In certain embodiments, the one or moremutations of RET include a substitution of Tyrosine (Y) withPhenylalanine (F) at amino acid 791. In certain embodiments, the one ormore mutations of RET include a substitution of Valine (V) withMethionine (M) at amino acid 804. In certain embodiments, the one ormore mutations of RET include a substitution of Methionine (M) withThreonine (T) at amino acid 918.

In alternative embodiments, the dTAGs for use in the present inventioninclude, but are not limited to, amino acid sequences derived fromproteins selected from Kit, Jak3, Abl, Mek1, HIV reverse transcriptase,and HIV integrase.

In an alternative embodiment, the dTAG for use in the present inventionis an amino acid sequence derived from LKKR2. In certain embodiments,the dTAG is a modified or mutant LKKR2 protein or fragment thereof. Incertain embodiments, the one or more mutations of LKKR2 include asubstitution of Arginine (R) with Cysteine (C) at amino acid 1441, asubstitution of Glycine (G) with Serine (S) at amino acid 2019, asubstitution of Isoleucine (I) with Threonine (T) at amino acid 2020.

In one embodiment, the dTAG has an amino acid sequence derived from aLRRK2 protein (UniProtKB-Q5S007 (LRKK2_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 1328 to 1511 of Q5S007. In one embodiment, the dTAG isderived from amino acid 1328 to 1511 of Q5S007, wherein amino acid 1441is Cysteine. In one embodiment, the dTAG is derived from amino acid 1328to 1511 of Q5S007 and the dTAG Targeting Ligand in theheterobifunctional compound is selected from a ligand in Table T-U1. Inone embodiment, the dTAG is derived from amino acid 1328 to 1511 ofQ5S007, wherein amino acid 1441 is Cysteine and the dTAG TargetingLigand in the heterobifunctional compound is selected from a ligand inTable T-U1. In one embodiment, the dTAG is derived from amino acid 1879to 2138 of Q5S007. In one embodiment, the dTAG is derived from aminoacid 1879 to 2138 of Q5S007, wherein amino acid 2019 is Serine. In onembodiment, the dTAG is derived from amino acid 1879 to 2138 of Q5S007,wherein amino acid 2020 is Threonine. In one embodiment, the dTAG isderived from amino acid 1879 to 2138 of Q5S007 and the dTAG TargetingLigand in the heterobifunctional compound is selected from a ligand inTable T-U2 or U3. In one embodiment, the dTAG is derived from amino acid1879 to 2138 of Q5S007, wherein amino acid 2019 is Serine and the dTAGTargeting Ligand in the heterobifunctional compound is selected from aligand in Table T-U2. In one embodiment, the dTAG is derived from aminoacid 1879 to 2138 of Q5 S007, wherein amino acid 2020 is Threonine andthe dTAG Targeting Ligand in the heterobifunctional compound is selectedfrom a ligand in Table T-U3.

In an alternative embodiment, the dTAG for use in the present inventionis an amino acid sequence derived from PDGFRα. In certain embodiments,the dTAG is a modified or mutant PDGFRa protein or fragment thereof. Incertain embodiments, the one or more mutations of PDGFRa include asubstitution of Threonine (T) with Isoleucine (I) at amino acid 6741.

In one embodiment, the dTAG has an amino acid sequence derived from aPDGFRa protein (UniProtKB-P09619 (PDGFR_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 600 to 692 of P09619. In one embodiment, the dTAG isderived from amino acid 600 to 692 of P09619, wherein amino acid 660 isAlanine. In one embodiment, the dTAG is derived from amino acid 600 to692 of P09619 and the dTAG Targeting Ligand in the heterobifunctionalcompound is selected from a ligand in Table T-V1. In one embodiment, thedTAG is derived from amino acid 600 to 692 of P09619, wherein amino acid660 is Alanine and the dTAG Targeting Ligand in the heterobifunctionalcompound is selected from a ligand in Table T-V1.

In an alternative embodiment, the dTAG for use in the present inventionis an amino acid sequence derived from RET. In certain embodiments, thedTAG is a modified or mutant RET protein or fragment thereof. In certainembodiments, the one or more mutations of RET include a substitution ofGlycine (G) with Serine (S) at amino acid 691. In certain embodiments,the one or more mutations of RET include a substitution of Arginine (R)with Threonine (T) at amino acid 691. In certain embodiments, the one ormore mutations of RET include a substitution of Glutamic acid (E) withGlutamine (Q) at amino acid 691. In certain embodiments, the one or moremutations of RET include a substitution of Tyrosine (Y) withPhenylalanine (F) at amino acid 691. In certain embodiments, the one ormore mutations of RET include a substitution of Valine (V) withMethionine (M) at amino acid 691. In certain embodiments, the one ormore mutations of RET include a substitution of Methionine (M) withThreonine (T) at amino acid 691.

In one embodiment, the dTAG has an amino acid sequence derived from aPDGFRa protein (UniProtKB-P07949 (RET_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 724 to 1016 of P07949. In one embodiment, the dTAG isderived from amino acid 724 to 1016 of P07949, wherein amino acid 940 isIsoleucine. In one embodiment, the dTAG is derived from amino acid 724to 1016 of P07949 and the dTAG Targeting Ligand in theheterobifunctional compound is selected from a ligand in Table T-W1. Inone embodiment, the dTAG is derived from amino acid 724 to 1016 ofP07949, wherein amino acid 940 is Isoleucine and the dTAG TargetingLigand in the heterobifunctional compound is selected from a ligand inTable T-W1. In one embodiment, the dTAG is derived from amino acid 724to 1016 of P07949 and the dTAG Targeting Ligand in theheterobifunctional compound is selected from a ligand in Table T-W2. Inone embodiment, the dTAG is derived from amino acid 724 to 1016 ofP07949 and the dTAG Targeting Ligand in the heterobifunctional compoundis selected from a ligand in Table T-W3. In one embodiment, the dTAG isderived from amino acid 724 to 1016 of P07949 and the dTAG TargetingLigand in the heterobifunctional compound is selected from a ligand inTable T-W4. In one embodiment, the dTAG is derived from amino acid 724to 1016 of P07949 and the dTAG Targeting Ligand in theheterobifunctional compound is selected from a ligand in Table T-W5. Inone embodiment, the dTAG is derived from amino acid 724 to 1016 ofP07949 and the dTAG Targeting Ligand in the heterobifunctional compoundis selected from a ligand in Table T-W6.

In a particular embodiment, the dTAG is derived from an amino acidsequence or fragment thereof of SEQ. ID. NO.: 53 and the dTAG is capableof being bound by a heterobifunctional compound that contains an EGFRdTAG Targeting Ligand selected from Table T-P1. In a particularembodiment, the dTAG is derived from an amino acid sequence or fragmentthereof of SEQ. ID. NO.: 54 and the dTAG is capable of being bound by aheterobifunctional compound that contains an EGFR dTAG Targeting Ligandselected from Table T-P2. In a particular embodiment, the dTAG isderived from an amino acid sequence or fragment thereof of SEQ. ID. NO.:55 and the dTAG is capable of being bound by a heterobifunctionalcompound that contains an EGFR dTAG Targeting Ligand selected from TableT-P3. In a particular embodiment, the dTAG is derived from an amino acidsequence or fragment thereof of SEQ. ID. NO.: 56 and the dTAG is capableof being bound by a heterobifunctional compound that contains an EGFRdTAG Targeting Ligand selected from Table T-P. In a particularembodiment, the dTAG is derived from an amino acid sequence or fragmentthereof of SEQ. ID. NO.: 57 and the dTAG is capable of being bound by aheterobifunctional compound that contains a BCR-ABL dTAG TargetingLigand selected from Table T-Q1. In a particular embodiment, the dTAG isderived from an amino acid sequence or fragment thereof of SEQ. ID. NO.:58 and the dTAG is capable of being bound by a heterobifunctionalcompound that contains a BCR-ABL dTAG Targeting Ligand selected fromTable T-Q1. In a particular embodiment, the dTAG is derived from anamino acid sequence or fragment thereof of SEQ. ID. NO.: 59 and the dTAGis capable of being bound by a heterobifunctional compound that containsa ALK dTAG Targeting Ligand selected from Table T-R1. In a particularembodiment, the dTAG is derived from an amino acid sequence or fragmentthereof of SEQ. ID. NO.: 60 and the dTAG is capable of being bound by aheterobifunctional compound that contains a JAK2 dTAG Targeting Ligandselected from Table T-S1. In a particular embodiment, the dTAG isderived from an amino acid sequence or fragment thereof of SEQ. ID. NO.:61 and the dTAG is capable of being bound by a heterobifunctionalcompound that contains a BRAF dTAG Targeting Ligand selected from TableT-T1.

In one embodiment, the dTAG is derived from LRRK2 amino acid 1328 to1511 (UnitPro-Q5S007) and the dTAG Targeting Ligand in theheterobifunctional compound is selected from a ligand in Table T-U1. Inone embodiment, the dTAG is derived from LRRK2 amino acid 1328 to 1511(UniProt-Q5S007), wherein amino acid 1441 is Cysteine and the dTAGTargeting Ligand in the heterobifunctional compound is selected from aligand in Table T-U1. In one embodiment, the dTAG is derived from LRRK2amino acid 1879 to 2138 (UniProt-Q5S007. In one embodiment, the dTAG isderived from LRRK2 amino acid 1879 to 2138 (UniProt-Q5S007), whereinamino acid 2019 is Serine. In on embodiment, the dTAG is derived fromamino acid 1879 to 2138 (UniProt-Q5S007), wherein amino acid 2020 isThreonine. In one embodiment, the dTAG is derived from LRRK2 amino acid1879 to 2138 (UniProt-Q5S007) and the dTAG Targeting Ligand in theheterobifunctional compound is selected from a ligand in Table T-U2 orU3. In one embodiment, the dTAG is derived from LRRK2 amino acid 1879 to2138 (UniProt-Q5S007), wherein amino acid 2019 is Serine and the dTAGTargeting Ligand in the heterobifunctional compound is selected from aligand in Table T-U2. In one embodiment, the dTAG is derived from LRRK2amino acid 1879 to 2138 (UniProt-Q5S007), wherein amino acid 2020 isThreonine and the dTAG Targeting Ligand in the heterobifunctionalcompound is selected from a ligand in Table T-U3. In one embodiment, thedTAG is derived from PDGFR amino acid 600 to 692 (UniProt-P09619) andthe dTAG Targeting Ligand in the heterobifunctional compound is selectedfrom a ligand in Table T-V1. In one embodiment, the dTAG is derived fromPDGFR amino acid 600 to 692 (UniProt-P09619), wherein amino acid 674 isIsoleucine and the dTAG Targeting Ligand in the heterobifunctionalcompound is selected from a ligand in Table T-V1. In one embodiment, thedTAG is derived from RET amino acid 724 to 1016 (UniProtKB-P07949), andthe dTAG Targeting Ligand in the heterobifunctional compound is selectedfrom a ligand in Table T-W1-W6. In one embodiment, the dTAG is derivedfrom RET amino acid 724 to 1016 (UniProtKB P07949), wherein amino acid691 is Serine and the dTAG Targeting Ligand in the heterobifunctionalcompound is selected from a ligand in Table T-W1. In one embodiment, thedTAG is derived from RET amino acid 724 to 1016 (UniProtKB-P07949),wherein amino acid 749 is Threonine and the dTAG Targeting Ligand in theheterobifunctional compound is selected from a ligand in Table T-W2. Inone embodiment, the dTAG is derived from RET amino acid 724 to 1016(UniProtKB-P07949), wherein amino acid 762 is Glutamine and the dTAGTargeting Ligand in the heterobifunctional compound is selected from aligand in Table T-W3. In one embodiment, the dTAG is derived from RETamino acid 724 to 1016 (UniProtKB-P07949), wherein amino acid 791 isPhenylalanine and the dTAG Targeting Ligand in the heterobifunctionalcompound is selected from a ligand in Table T-W4. In one embodiment, thedTAG is derived from RET amino acid 724 to 1016 (UniProtKB-P07949),wherein amino acid 804 is Methionine and the dTAG Targeting Ligand inthe heterobifunctional compound is selected from a ligand in Table T-W5.In one embodiment, the dTAG is derived from RET amino acid 724 to 1016(UniProtKB-P07949), wherein amino acid 918 is Threonine and the dTAGTargeting Ligand in the heterobifunctional compound is selected from aligand in Table T-W6. In one embodiment, the dTAG is derived from anJAK2, and the dTAG Targeting Ligand in the heterobifunctional compoundis selected from a ligand in Table T-JJJ1. In one embodiment, the dTAGis derived from an Abl, and the dTAG Targeting Ligand in theheterobifunctional compound is selected from a ligand in Table T-KKK1.In one embodiment, the dTAG is derived from an MEK1, and the dTAGTargeting Ligand in the heterobifunctional compound is selected from aligand in Table T-LLL1. In one embodiment, the dTAG is derived from anKIT, and the dTAG Targeting Ligand in the heterobifunctional compound isselected from a ligand in Table T-MMM1. In one embodiment, the dTAG isderived from an HIV reverse transcriptase, and the dTAG Targeting Ligandin the heterobifunctional compound is selected from a ligand in TableT-NNN1. In one embodiment, the dTAG is derived from an HIV integrase,and the dTAG Targeting Ligand in the heterobifunctional compound isselected from a ligand in Table T-OOO1.

In a particular embodiment, the dTAG is derived from a protein selectedfrom EGFR, ErbB2, ErbB4, VEGFR1, VEGFR2, VEGFR3, Kit, BCR-Abl, Src, Lyn,Hck, RET, c-Met, TrkB, Flt3, Axl, Tie2, ALK, IGF-1R, InsR, ROS1, MST1R,B-Raf, Lck, Yes, Fyn, HER2-breast cancer, PNET, RCC, RAML, SEGA, BTK,FGFR1/2/3/4, DDR1, PDGFRα, PDGFRP3, CDK4, CDK6, Fms, Itk, T315I, Eph2A,JAK1, JAK2, JAK3 CDK8, CSF-1R, FKBP12/mTOR, MEK1, MEK2, Brk, EphR,A-Raf, B-Raf, C-Raf and the heterobifunctional compound contains a dTAGTargeting Ligand selected from Table Z.

Heterobifunctional compounds capable of binding to the amino acidsequences, or a fragment thereof, described above can be generated usingthe dTAG Targeting Ligands described in Table T. In one embodiment, anucleic acid sequence encoding a dTAG derived from an amino acidsequence described above, or a fragment thereof, is genomically insertedinto a gene encoding an endogenous protein of interest which, uponexpression, results in an endogenous protein-dTAG hybrid protein and isdegraded by administering to the subject a heterobifunctional compoundcomprising a dTAG Targeting Ligand described in Table T. In oneembodiment, a nucleic acid sequence encoding a dTAG derived from anamino acid sequence described above, or a fragment thereof, isgenomically inserted into a gene encoding an endogenous protein ofinterest which, upon expression, results in an endogenous protein-dTAGhybrid protein and is degraded by administering to the subject itscorresponding heterobifunctional compound, which is capable of bindingto the dTAG, for example a heterobifunctional compound described in FIG.29, FIG. 30, FIG. 31, FIG. 32, and FIG. 33, or any otherheterobifunctional compound described herein.

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1 is a schematic representing a “bump-hole” approach for selectivedegradation of a dTAG fusion protein. For example, the dTAG fusion canbe a version of the FK506- and Rapamycin-binding protein FKBP12engineered with a cavity forming “hole” via an amino acid mutation(F36V). This mutant FKBP12 (“bumped” FKBP, aka FKBP* or FKBP12* (SEQ.ID. NO.: 2)) can then be selectively targeted by a heterobifunctionalcompound possessing a synthetic “bump” in the FKBP12 binding domain, alinker, and a cereblon targeting domain. This heterobifunctionalcompound does not target native FKBP12 and thus offers selectivityagainst wildtype variants of the tag naturally present in human cells.

FIG. 2 is a schematic representing the genomic integration of a nucleicacid sequence encoding a dTAG into the genomic locus of the endogenousgene encoding PCSK9. Following homologous recombination, the resultantinsertion results in an expression product comprising an N-terminus dTAGin frame with the proprotein convertase subtilisin/kexin type 9 (PCSK9)protein, thus providing a proprotein convertase subtilisin/kexin type 9(PCSK9)-dTAG hybrid capable of being degraded by a heterobifunctionalcompound targeting the dTAG sequence.

FIG. 3 is a schematic representing the genomic integration of a nucleicacid sequence encoding a dTAG into the genomic locus of the endogenousgene encoding 3-catenin (CTNNB1). Following homologous recombination,the resultant insertion results in an expression product comprising anN-terminus dTAG in frame with the β-catenin (CTNNB1) protein, thusproviding a β-catenin (CTNNB1)-dTAG hybrid capable of being degraded bya heterobifunctional compound targeting the dTAG sequence.

FIG. 4 is an immunoblot of cells treated with heterobifunctionalcompounds described in the present invention. 293FT cells (CRBN-WT orCRBN−/−) expressing either HA-tagged FKBP12WT or FKBP* were treated withindicated concentrations of dFKBP7 for 4 hours. CRBN-dependentdegradation of FKBP* and not FKBPWT confirms selective activity ofdFKBP7 for mutant FKBP*.

FIG. 5A and FIG. 5B are graphs measuring the activity of a panel ofdFKBP heterobifunctional compounds in cells expressing FKBP* fused toNluc. Degradation of FKBP* is measured as a signal ration (Nluc/Fluc)between NANOluc and firefly luciferase from the same multicistronictranscript in wild type (FIG. 7A) or CRBN −/− (FIG. 7B) 293FT cellstreated with indicated concentrations of dFKBPs for 4 hours. A decreasein the signal ratio indicates FKBP* (Nluc) degradation.

FIG. 6 is an immunoblot of cells treated with heterobifunctionalcompounds described in the present invention. Isogenic 293FT cells(CRBN-WT or CRBN−/−) expressing either FKBP12WT or FKBP* were treatedwith 100 nM of either dFKBP7 or dFKBP13 for 4 hours. CRBN-dependentdegradation of FKBP* and not FKBP12WT or endogenous FKBP12 confirmsselectivity of dFKBP7 and dFKBP13 for mutant FKBP*

FIG. 7 is an immunoblot of cells treated with heterobifunctionalcompounds described in the present invention. Isogenic 293FT cells(CRBN-WT or CRBN−/−) expressing HA-tagged FKBP* were treated with theindicated dose of dFKBP13 for 4 hours. These data confirm dose- andCRBN-dependent degradation of HA-tagged FKBP* by dFKBP13.

FIG. 8 is an immunoblot of cells treated with heterobifunctionalcompounds described in the present invention. 293FT cells (CRBN-WT)expressing HA-tagged FKBP* were treated with 100 nM dFKBP13 for theindicated times. Cells were harvested and protein lysates immunoblottedto measure the kinetics of HA-tagged FKBP* degradation induced bydFKBP13.

FIG. 9 is an immunoblot of cells treated with heterobifunctionalcompounds described in the present invention. 293FT cells (CRBN-WT)expressing FKBP* were pretreated with 1 uM Carfilzomib (proteasomeinhibitor), 0.5 uM MLN4924 (neddylation inhibitor), and 10 uMLenalidomide (CRBN binding ligand) for two hours prior to a 4 hourtreatment with dFKBP13. Degradation of HA-tagged FKBP* by dFKBP13 wasrescued by the proteasome inhibitor Carfilzomib, establishing arequirement for proteasome function. Pre-treatment with the NAE1inhibitor MLN4924 rescued HA-tagged FKBP* establishing dependence on CRLactivity, as expected for cullin-based ubiquitin ligases that requireneddylation for processive E3 ligase activity. Pre-treatment with excessLenalidomide abolished dFKBP13-dependent FKBP* degradation, confirmingthe requirement of CRBN engagement for degradation.

FIG. 10A and FIG. 10B are immunoblots of cells treated withheterobifunctional compounds described in the present invention.Immunoblots of MV4; 11 leukemia cells expressing indicated proteinsfused to mutant FKBP* with an HA tag. Cells were treated for 16 hourswith indicated concentrations of FKBP* selective heterobifunctionalcompounds, dFKBP7 or dFKBP13 and abundance of fusion proteins measuredby western immunoblot analysis.

FIG. 11 is an immunoblot of NIH3T3 cells expressing KRASG12V allelefused to FKBP* in the N-terminus or C-terminus. Cells were treated with500 nM dFKBP7 for the indicated time. Cells were harvested andimmunoblotted to measure degradation of FKBP*-KRASG12V and downstreamsurrogates of KRAS signaling (e.g. pMEK and pAKT). The data suggestN-terminal FKBP* fusions are active and degraded upon administration ofdFKBP7.

FIG. 12 is an immunoblot of NIH3T3 cells expressing FKBP* fused to theN-terminus of KRASG12V treated with 1 uM of the indicated dFKBPheterobifunctional compounds for 24 hours. Cells were harvested andimmunoblotted to measure degradation of FKBP*-KRASG12V and downstreamsurrogates of KRAS signaling (e.g. pMEK and pAKT). The data suggest thatdFKBP9, dFKBP12, and dFKBP13 induce potent degradation of FKBP*-KRASG12Vand inhibition of downstream signaling.

FIG. 13 is an immunoblot of NIH3T3 cells expressing FKBP* fused to theN-terminus of KRASG12V treated with the indicated concentrations ofdFKBP13 for 24 hours. Cells were harvested and immunoblotted to measuredegradation of FKBP*-KRASG12V and downstream surrogates of KRASsignaling (e.g. pMEK and pAKT). The data suggest that dFKBP13 inducespotent degradation of FKBP*-KRASG12V and inhibits downstream signalingpotently with an IC50>100 nM.

FIG. 14 is an immunoblot of NIH3T3 cells expressing FKBP* fused to theN-terminus of KRASG12V treated with 1 uM dFKBP13 for the indicated time.Cells were harvested and immunoblotted to measure degradation ofFKBP*-KRASG12V and downstream surrogates of KRAS signaling (e.g. pMEKand pAKT). Data suggest that dFKBP13 induces potent degradation ofFKBP*-KRASG12V and inhibition of downstream signaling as early as 1 hourpost treatment.

FIG. 15 is an immunoblot of NIH3 T3 cells expressing dTAG-KRASG12Vpretreated with 1 uM Carfilzomib (proteasome inhibitor), 0.5 uM MLN4924(neddylation inhibitor), and 10 uM Lenalidomide (CRBN binding ligand)for two hours prior to a 4 hour treatment with dFKBP13.

FIG. 16 is an immunoblot of NIH3T3 cells expressing KRAS alleles eitherWT or mutant forms of amino acid glycine 12 (G12C, G12D, and G12V)treated with 1 uM of dFKBP13 for 24 hours.

FIG. 17 is an immunoblot of NIH3T3 cells expressing either WT or mutantKRAS alleles (G13D, Q61L, and Q61R) treated with 1 uM of dFKBP13 for 24hours.

FIG. 18A, FIG. 18B, FIG. 18C, and FIG. 18D are panels of phase contrastimages of control NIH3T3 cells or NIH3T3 expressing FKBP* fused to theN-terminus of KRASG12V treated with DMSO of dFKBP13 for 24 hours. Phasecontrast images highlight the morphological change induced upondFKBP13-dependent degradation of FKBP*-KRASG12V.

FIG. 19A, FIG. 19B, FIG. 19C, and FIG. 19D are proliferation graphs thatmeasure the effect of dFKBP13 on the growth of NIH3T3 control cells ofNIH3T3 expressing FKBP*-KRASG12V. Cells were treated with the indicatedconcentrations if dFKBPs for 72 hours and cell count measured using anATPlite assay. The ATPlite 1step luminescence assay measures cellproliferation and cytotoxicity in cells based on the production of lightcaused by the reaction of ATP with added luciferase and D-luciferin. Adecrease in signal indicates a reduction in cell number.

FIG. 20 is a bar graph illustrating NIH3T3 cells expressingdTAG-KRASG12V treated with dFKBP7 and dFKBP13 for 48 hours to inducetargeted dTAG-KRASG12V degradation. Fixed cells were stained withpropidium iodide and cell cycle analysis was performed.

FIG. 21A, FIG. 21B, FIG. 21C, FIG. 21D, FIG. 21E, FIG. 21F, FIG. 21G,FIG. 21H, and FIG. 21I provide examples of Degron moieties for use inthe present invention, wherein R is the point of attachment for theLinker and X is as defined herein.

FIG. 22 provides additional examples of Degron moieties for use in thepresent invention, wherein R is the point of attachment for the Linkerand X is as defined herein.

FIG. 23 provides additional examples of Degron moieties for use in thepresent invention, wherein R is the point of attachment for the Linkerand X is as defined herein.

FIG. 24 provides examples of Linker moieties for use in the presentinvention.

FIG. 25 provides additional examples of Linker moieties for use in thepresent invention.

FIG. 26 provides examples of heteroaliphatic Linker moieties for use inthe present invention.

FIG. 27 provides examples of aromatic Linker moieties for use in thepresent invention.

FIG. 28A, FIG. 28B, FIG. 28C, FIG. 28D, FIG. 28E, FIG. 28F, and FIG. 28Gprovide dTAG Targeting Ligands for use in the present invention, whereinR is the point at which the Linker is attached.

FIG. 29A, FIG. 29B, FIG. 29C, FIG. 29D, FIG. 29E, FIG. 29F, FIG. 29G,and FIG. 29H provide specific heterobifunctional compounds for use inthe present invention.

FIG. 30A, FIG. 30B, FIG. 30C, FIG. 30D, FIG. 30E, FIG. 30F, FIG. 30G,FIG. 30H, FIG. 30I, FIG. 30J, FIG. 30K, FIG. 30L, FIG. 30M, FIG. 30N,FIG. 30O, and FIG. 30P provides specific heterobifunctional compoundsfor use in the present invention, wherein X in the above structures is ahalogen chosen from F, Cl, Br, and I.

FIG. 31A, FIG. 31B, FIG. 31C, FIG. 31D, FIG. 31E, FIG. 31F, FIG. 31G,FIG. 31H, FIG. 31I, and FIG. 31J provide specific heterobifunctionalcompounds for use in the present invention.

FIG. 32S, FIG. 32B, FIG. 32C, FIG. 32D, FIG. 32E, FIG. 32F, FIG. 32G,FIG. 32H, FIG. 32I, FIG. 32J, FIG. 32K, FIG. 32L, FIG. 32M, FIG. 32N,FIG. 32O, FIG. 32P, FIG. 32Q, FIG. 32R, FIG. 32S, FIG. 32T, FIG. 32U,FIG. 32V, FIG. 32W, FIG. 32X, FIG. 32Y, FIG. 32Z, FIG. 32AA, FIG. 32BB,FIG. 32CC, FIG. 32DD, and FIG. 32EE provide specific heterobifunctionalcompounds for use in the present invention, wherein R^(AR1) and R^(AR2)are described herein.

FIG. 33A, FIG. 33B, FIG. 33C, FIG. 33D, FIG. 33E, FIG. 33F, FIG. 33G,FIG. 33H, FIG. 33I, FIG. 33J, FIG. 33K, FIG. 33L, FIG. 33M, FIG. 33N,FIG. 33O, FIG. 33P, FIG. 33Q, FIG. 33R, FIG. 33S, FIG. 33T, FIG. 33U,FIG. 33V, and FIG. 33W provide additional heterobifunctional compoundsfor use in the present invention.

DETAILED DESCRIPTION OF THE INVENTION

Practice of the methods, as well as preparation and use of thecompositions disclosed herein employ, unless otherwise indicated,conventional techniques in molecular biology, biochemistry, chromatinstructure and analysis, computational chemistry, cell culture,recombinant DNA and related fields as are within the skill of the art.These techniques are fully explained in the literature. See, forexample, Sambrook et al. MOLECULAR CLONING: A LABORATORY MANUAL, Secondedition, Cold Spring Harbor Laboratory Press, 1989 and Third edition,2001; Ausubel et al., CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, John Wiley& Sons, New York, 1987 and periodic updates; the series METHODS INENZYMOLOGY, Academic Press, San Diego; Wolffe, CHROMATIN STRUCTURE ANDFUNCTION, Third edition, Academic Press, San Diego, 1998; METHODS INENZYMOLOGY, Vol. 304, “Chromatin” (P. M. Wassarman and A. P. Wolffe,eds.), Academic Press, San Diego, 1999; and METHODS IN MOLECULARBIOLOGY, Vol. 119, “Chromatin Protocols” (P. B. Becker, ed.) HumanaPress, Totowa, 1999.

Here, we describe a method that takes advantage of both gene and proteindisruption to provide a highly selective and reversible method forpromoting protein degradation. This methodology is of value for precise,temporal, small-molecule controlled target validation and theexploration of cellular and in vivo effects of protein of interestdegradation.

In this method, a region of the target gene of interest is targeted by aguide RNA and Cas9 in order to insert (knock-in) an expression cassettefor dTAG present in a homologous recombination (HR) targeting vector.The HR targeting vector contains homology arms at the 5′ and 3′ end ofthe expression cassette homologous to the genomic DNA surrounding thetargeting gene of interest locus. By fusing dTAG in frame with thetarget gene of interest, the resulting fusion protein upon expressionwill be made susceptible to proteasome mediated degradation upontreatment with a bioinert small molecule heterobifunctional compound.

Genome editing in mammalian cells offers much potential for thetreatment and correction of human disease. By using short single-guideRNAs (sgRNAs) the Cas9 endonuclease can be directed to genomic positionsof interest whereupon it induces DNA double strand breaks. These breaksare repaired by non-homologous end joining, which can be leveraged toproduce insertions or deletions (indels) that inactivate genes. In vivogenome editing can be accomplished with CRISPR/Cas9 delivery byadeno-associated virus (AAV-), lentivirus-, particle-, hydrodynamicinjection- or electroporation-mediated methods, or combinations thereof(see, for example, Kumar et al., Hum. Gene Ther. 12, (2001):1893-1905;Wu et al., Mol. Ther. 18, (2010):80-86; Ran et al., Nature 520, (2015):186-191; Swiech et al., Nat. Biotechnol. 33, (2015):102-105; Zuris etal., Nat. Biotechnol. 33, (2015):73-80; Kauffman et al., Nano. Lett. 15,(2015):7300-7306; Ding et al., Circ. Res. 115, (2014):488-492; Mareschet al., Nat. Commun. 7, (2016):10770; Khorsandi et al., Cancer GeneTher. 15, (2008):225-230; Yin et al., Nat. Rev. Genet. 15,(2014):541-555; Yin et al., Nat. Biotechnol. 34, (2016):328-333; and Xueet al., Nature 514, (2014):380-384, incorporated herein by reference)and somatic genome editing has been applied to mouse organs such as thelung, liver, brain, and pancreas (see, for example, Xue et al., Nature514, (2014):380-384; Sanchez-Rivera et al., Nature 516, (2014):428-431;Platt et al., Cell 159, (2014):440-455; Yin et al., Nat. Biotechnol. 32,(2014):551-553; Zuckermann et al., Nat. Commun. 6, (2015):7391; Chiou etal., Genes Dev. 29, (2015):1576-1585; and Mazur et al., Nat. Med. 21,(2015):1163-1171, incorporated herein by reference). However, thelong-term implications of permanent genome modification are unknown andconcerns exist over the imperfect precision of genome editing and theimpact of direct correction in adults where biological compensationmechanisms may exist (see, for example, Fu et al., Nat. Biotechnol.31(9), (2013):822-826, and Cho et al., Genome Res. 24, (2014):132-141,incorporated herein by reference).

Here we describe a strategy for widespread therapeutic use that is basedon in vivo genome engineering to produce knock-in fusion proteins thatare produced from the endogenous locus and are readily degraded in aligand-dependent, reversible, and dose-responsive, fashion. The fusionprotein contains a dTAG that is targeted by a bi- or polyvalentheterobifunctional compound. The heterobifunctional compound has theability to bind the dTAG and recruit an E3 ligase e.g. thecereblon-containing CRL4A E3 ubiquitin ligase complex. This recruitmentinduces ubiquitination of the fusion protein (on either the dTAG domainor on the cognate protein) and subsequent degradation via the UPP.Through this approach a protein of interest can be targeted for rapidubiquitin mediated degradation with high specificity and highspecificity without requiring the discovery of a de novo ligand for theprotein of interest. In light of the combined use of a small moleculeand genome engineering for in vivo use.

A variety of dTAGs can be used, including, but not limited to,bromodomains e.g. the first bromodomain of BRD4; hormone receptors e.g.ER, AR, RXR; FKBP12; DHFR, esp. bacterial DHFR, and other commonly usedprotein fusion tags that can be bound by a ligand that can be convertedto a heterobifunctional compound. In some cases, there will be anadvantage to using a dTAG that leverages a “bump-hole” strategyconceptually related to that developed to selectively target the ATPbinding site of protein kinases. In such a case, the dTAG fusion is aversion of the FK506- and Rapamycin-binding protein FKBP12 engineeredwith a cavity forming “hole” via an amino acid mutation (F36V). Thismutant FKBP12 (“bumped” FKBP, aka FKBP* (SEQ. ID. NO.: 2) is thentargeted by a heterobifunctional compound (or similar molecule)possessing a synthetic “bump” in the FKBP12 binding domain, a linker,and a cereblon targeting domain (e.g. an IMID derivative). This moleculedoes not target native FKBP12 and thus offers selectivity of theheterobifunctional compound against wildtype variants of the tagnaturally present in human cells. An illustration representing theexemplified “bump-hole” strategy is provided for in FIG. 1.

The invention described herein provides a mechanism to control thedegradation of endogenous proteins of relevance to disease by combininggenome engineering with small molecule activation/modulation ofdegradation. Applications of this technology include, but are notlimited to 1) targeted degradation of proteins where pathology is afunction of gain of function mutation(s), 2) targeted degradation ofproteins where pathology is a function of amplification or increasedexpression, 3) targeted degradation of proteins that are manifestationsof monogenetic disease, 4) targeted degradation of proteins wheregenetic predisposition manifests over longer periods and often afteralternative biological compensatory mechanisms are no longer adequate,e.g. hypercholesterolemia, proteinopathies.

Definitions

The terms “nucleic acid,” “polynucleotide,” and “oligonucleotide” areused interchangeably and refer to a deoxyribonucleotide orribonucleotide polymer, in linear or circular conformation, and ineither single- or double-stranded form. For the purposes of the presentdisclosure, these terms are not to be construed as limiting with respectto the length of a polymer. The terms can encompass known analogues ofnatural nucleotides, as well as nucleotides that are modified in thebase, sugar and/or phosphate moieties (e.g., phosphorothioatebackbones). In general, an analogue of a particular nucleotide has thesame base-pairing specificity; i.e., an analogue of A will base-pairwith T.

The terms “polypeptide,” “peptide,” and “protein” are usedinterchangeably to refer to a polymer of amino acid residues. The termalso applies to amino acid polymers in which one or more amino acids arechemical analogues or modified derivatives of correspondingnaturally-occurring amino acids.

“Binding” refers to a sequence-specific, non-covalent interactionbetween macromolecules (e.g., between a protein and a nucleic acid) or amacromolecule and a small molecule (e.g. between a protein and a drug).Not all components of a binding interaction need be sequence-specific(e.g., contacts with phosphate residues in a DNA backbone), as long asthe interaction as a whole is sequence-specific.

“Recombination” refers to a process of exchange of genetic informationbetween two polynucleotides. For the purposes of this disclosure,“homologous recombination” (HR) refers to the specialized form of suchexchange that takes place, for example, during repair of double-strandbreaks in cells via homology-directed repair mechanisms. This processrequires nucleotide sequence homology, uses a “donor” molecule totemplate repair of a “target” molecule (i.e., the one that experiencedthe double-strand break), and leads to the transfer of geneticinformation from the donor to the target.

One or more targeted nucleases as described herein create adouble-stranded break in the target sequence (e.g., cellular chromatin)at a predetermined site, and a “donor” polynucleotide, encoding a dTAG,having homology to the nucleotide sequence in the region of the break,can be introduced into the cell. The presence of the double-strandedbreak has been shown to facilitate integration of the donor sequence.The donor sequence may be physically integrated, resulting in theintroduction of all or part of the nucleotide sequence as in the donorinto the cellular chromatin. Thus, a first sequence in cellularchromatin can be altered and converted into a sequence present in adonor polynucleotide.

In certain methods for targeted recombination and/or replacement and/oralteration of a sequence in a region of interest in cellular chromatin,a chromosomal sequence is altered by homologous recombination with anexogenous “donor” nucleotide sequence encoding a dTAG. Such homologousrecombination is stimulated by the presence of a double-stranded breakin cellular chromatin, if sequences homologous to the region of thebreak are present.

In any of the methods described herein, the exogenous nucleotidesequence (the “donor sequence” or “transgene”) can contain sequencesthat are homologous, but not identical, to genomic sequences in theregion of interest, thereby stimulating homologous recombination toinsert a non-identical sequence, i.e., the nucleic acid sequenceencoding a dTAG, in the region of interest. Thus portions of the donorsequence that are homologous to sequences in the region of interestexhibit between about 80 to 99% (or any integer there between) sequenceidentity to the genomic sequence that is replaced. In other embodiments,the homology between the donor and genomic sequence is higher than 99%,for example if only 1 nucleotide differs as between donor and genomicsequences of over 100 contiguous base pairs. A non-homologous portion ofthe donor sequence contains nucleic sequences not present in the regionof interest, e.g., a sequence encoding a dTAG, such that new sequencesare introduced into the region of interest. In these instances, thenon-homologous sequence is generally flanked by sequences of 50-1,000base pairs (or any integral value there between) or any number of basepairs greater than 1,000, that are homologous or identical to sequencesin the region of interest. In other embodiments, the donor sequence isnon-homologous to the first sequence, and is inserted into the genome bynon-homologous recombination mechanisms.

“Cleavage” refers to the breakage of the covalent backbone of a DNAmolecule. Cleavage can be initiated by a variety of methods including,but not limited to, enzymatic or chemical hydrolysis of a phosphodiesterbond. Both single-stranded cleavage and double-stranded cleavage arepossible, and double-stranded cleavage can occur as a result of twodistinct single-stranded cleavage events. DNA cleavage can result in theproduction of either blunt ends or staggered ends. In certainembodiments, fusion polypeptides are used for targeted double-strandedDNA cleavage.

“Chromatin” is the nucleoprotein structure comprising the cellulargenome. Cellular chromatin comprises nucleic acid, primarily DNA, andprotein, including histones and non-histone chromosomal proteins. Themajority of eukaryotic cellular chromatin exists in the form ofnucleosomes, wherein a nucleosome core comprises approximately 150 basepairs of DNA associated with an octamer comprising two each of histonesH2A, H2B, H3 and H4; and linker DNA (of variable length depending on theorganism) extends between nucleosome cores. A molecule of histone H1 isgenerally associated with the linker DNA. For the purposes of thepresent disclosure, the term “chromatin” is meant to encompass all typesof cellular nucleoprotein, both prokaryotic and eukaryotic. Cellularchromatin includes both chromosomal and episomal chromatin.

An “exogenous” molecule is a molecule that is not normally present in acell, for example, certain dTAGs but can be introduced into a cell byone or more genetic, biochemical or other methods. An exogenous moleculecan comprise, for example, a synthetic endogenous protein-dTAG hybrid.

An “endogenous” protein is one that is normally present in a particularcell at a particular developmental stage under particular environmentalconditions. For example, an endogenous protein, for example, may be atranscription factor or enzyme or any other type of naturally expressedprotein.

A “fusion” or “hybrid” protein is a protein in which two or morepolypeptides are linked, preferably covalently. Examples of fusionproteins, for example, include a fusion between an endogenous proteinand a dTAG.

A “gene,” for the purposes of the present disclosure, includes a DNAregion encoding a gene product, as well as all DNA regions whichregulate the production of the gene product, whether or not suchregulatory sequences are adjacent to coding and/or transcribedsequences. Accordingly, a gene includes, but is not necessarily limitedto, promoter sequences, terminators, translational regulatory sequencessuch as ribosome binding sites and internal ribosome entry sites,enhancers, silencers, insulators, boundary elements, replicationorigins, matrix attachment sites and locus control regions.

“Gene expression” refers to the conversion of the information, containedin a gene, into a gene product. A gene product can be the directtranscriptional product of a gene (e.g., mRNA, tRNA, rRNA, antisenseRNA, ribozyme, structural RNA or any other type of RNA) or a proteinproduced by translation of an mRNA. Gene products also include RNAswhich are modified, by processes such as capping, polyadenylation,methylation, and editing, and proteins modified by, for example,methylation, acetylation, phosphorylation, ubiquitination,ADP-ribosylation, myristilation, and glycosylation.

“Modulation” of protein expression refers to a change in the activity ofa protein. Modulation of expression can include, but is not limited to,reduced protein activity or increased protein activity. For example, ascontemplated herein, exposing an endogenous protein-dTAG hybrid to aheterobifunctional compound, resulting in the degradation of theendogenous protein-dTAG hybrid, may modulate the activity of theendogenous protein. Thus, protein inactivation may be partial orcomplete.

A “vector” is capable of transferring gene sequences to target cells.Typically, “vector construct,” “expression vector,” and “gene transfervector,” mean any nucleic acid construct capable of directing theexpression of a gene of interest and which can transfer gene sequencesto target cells. Thus, the term includes cloning, and expressionvehicles, as well as integrating vectors.

The terms “subject” and “patient” are used interchangeably and refer tomammals such as human patients and non-human primates, as well asexperimental animals such as rabbits, dogs, cats, rats, mice, rabbitsand other animals. Accordingly, the term “subject” or “patient” as usedherein means any patient or subject (e.g., mammalian) having a disorder.

A. Heterobifunctional Compound Targeting Protein (dTAGs)

The present invention provides method for making knock-in fusionproteins that are produced from the endogenous locus and are readilydegraded in a ligand-dependent, reversible, and dose-responsive,fashion. Specifically, a nucleic acid encoding a dTAG is inserted inframe with a target gene of interest, wherein upon expression, theresulting fusion protein contains a dTAG that is targeted by a bi- orpolyvalent heterobifunctional compound. The heterobifunctional compoundhas the ability to bind the target protein and recruit an E3 ligase e.g.the cereblon-containing CRL4A E3 ubiquitin ligase complex. Thisrecruitment induces ubiquitination of the fusion protein (on either thedTAG or on the cognate protein) and subsequent degradation via theubiquitin proteasome pathway (UPP). Through this approach a protein ofinterest can be targeted for rapid ubiquitin mediated degradation withhigh specificity without requiring the discovery of a de novo ligand forthe POI.

The heterobifunctional compound targeting protein of the synthetic geneis any amino acid sequence to which a heterobifunctional compound can bebound, leading to the ubiquitination and degradation of the expressedendogenous protein-dTAG hybrid protein when in contact with theheterobifunctional compound. Preferably, the dTAG should not interferewith the function of the endogenously expressed protein. In oneembodiment, the dTAG is a non-endogenous peptide, leading toheterobifunctional compound selectivity and allowing for the avoidanceof off target effects upon administration of the heterobifunctionalcompound. In one embodiment, the dTAG is an amino acid sequence derivedfrom an endogenous protein or fragment thereof which has been modifiedso that the heterobifunctional compound binds only to the modified aminoacid sequence and not the endogenously expressed protein. In oneembodiment, the dTAG is an endogenously expressed protein or a fragmentof an endogenously expressed protein. Any amino acid sequence domainthat can be bound by a ligand for use in a heterobifunctional compoundcan be used as a dTAG as contemplated herewith. In certain embodiments,it is preferred that the smallest amino acid sequence capable of beingbound by a particular heterobifunctional compound be utilized as a dTAG.

In particular embodiments, the dTAG for use in the present inventioninclude, but are not limited to, an amino acid sequence derived from anendogenously expressed protein such as FK506 binding protein-12(FKBP12), bromodomain-containing protein 4 (BRD4), CREB binding protein(CREBBP), and transcriptional activator BRG1 (SMARCA4), or a variantthereof. As contemplated herein, “variant” means any variant comprisinga substitution, deletion, or addition of one or a few to plural aminoacids, provided that the variant substantially retains the same functionas the original sequence, which in this case is providing a ligand for aheterobifunctional compound. In other embodiments, a dTAG for use in thepresent invention may include, for example, a hormone receptor e.g.estrogen-receptor protein, androgen receptor protein, retinoid xreceptor (RXR) protein, and dihydrofolate reductase (DHFR), includingbacterial DHFR, bacterial dehydrogenase, and variants.

Some embodiments of dTAGs can be, but are not limited to, those derivedfrom Hsp90 inhibitors, kinase inhibitors, MDM2 inhibitors, compoundstargeting Human BET Bromodomain-containing proteins, compounds targetingcytosolic signaling protein FKBP12, HDAC inhibitors, human lysinemethyltransferase inhibitors, angiogenesis inhibitors, immunosuppressivecompounds, and compounds targeting the aryl hydrocarbon receptor (AHR).

In certain embodiments, the dTAG is derived from, a kinase, a BETbromodomain-containing protein, a cytosolic signaling protein (e.g.,FKBP12), a nuclear protein, a histone deacetylase, a lysinemethyltransferase, a protein regulating angiogenesis, a proteinregulating immune response, an aryl hydrocarbon receptor (AHR), anestrogen receptor, an androgen receptor, a glucocorticoid receptor, or atranscription factor (e.g., SMARCA4, SMARCA2, TRIM24).

In certain embodiments, the dTAG is derived from a kinase, for example,but not limited to, a tyrosine kinase (e.g., AATK, ABL, ABL2, ALK, AXL,BLK, BMX, BTK, CSF1R, CSK, DDR1, DDR2, EGFR, EPHA1, EPHA2, EPHA3, EPHA4,EPHA5, EPHA6, EPHA7, EPHA8, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB6,ERBB2, ERBB3, ERBB4, FER, FES, FGFR1, FGFR2, FGFR3, FGFR4, FGR, FLT1,FLT3, FLT4, FRK, FYN, GSG2, HCK, IGF1R, ILK, INSR, INSRR, IRAK4, ITK,JAK1, JAK2, JAK3, KDR, KIT, KSR1, LCK, LMTK2, LMTK3, LTK, LYN, MATK,MERTK, MET, MLTK, MST1R, MUSK, NPR1, NTRK1, NTRK2, NTRK3, PDGFRA,PDGFRB, PLK4, PTK2, PTK2B, PTK6, PTK7, RET, ROR1, ROR2, ROS1, RYK,SGK493, SRC, SRMS, STYK1, SYK, TEC, TEK, TEX14, TIE1, TNK1, TNK2,TNNI3K, TXK, TYK2, TYRO3, YES 1, or ZAP70), a serine/threonine kinase(e.g., casein kinase 2, protein kinase A, protein kinase B, proteinkinase C, Raf kinases, CaM kinases, AKT 1, AKT2, AKT3, ALK1, ALK2, ALK3,ALK4, Aurora A, Aurora B, Aurora C, CHK1, CHK2, CLK1, CLK2, CLK3, DAPK1,DAPK2, DAPK3, DMPK, ERK1, ERK2, ERK5, GCK, GSK3, HIPK, KHS1, LKB1, LOK,MAPKAPK2, MAPKAPK, MNK1, MSSK1, MST1, MST2, MST4, NDR, NEK2, NEK3, NEK6,NEK7, NEK9, NEK11, PAK1, PAK2, PAK3, PAK4, PAK5, PAK6, PIM1, PIM2, PLK1,RIP2, RIP5, RSK1, RSK2, SGK2, SGK3, SIK1, STK33, TAO1, TAO2, TGF-beta,TLK2, TSSK1, TSSK2, ULK1, or ULK2), a cyclin dependent kinase (e.g.,Cdk1-Cdk11), and a leucine-rich repeat kinase (e.g., LRRK2).

In certain embodiments, the dTAG is derived from a BETbromodomain-containing protein, for example, but not limited to, ASH1L,ATAD2, BAZ1A, BAZ1B, BAZ2A, BAZ2B, BRD1, BRD2, BRD3, BRD4, BRD5, BRD6,BRD7, BRD8, BRD9, BRD10, BRDT, BRPF1, BRPF3, BRWD3, CECR2, CREBBP,EP300, FALZ, GCN5L2, KIAA1240, LOC93349, MLL, PB1, PCAF, PHIP, PRKCBP1,SMARCA2, SMARCA4, SP100, SP110, SP140, TAF1, TAF1L, TIFla, TRIM28,TRIM33, TRIM66, WDR9, ZMYND11, and MLL4. In certain embodiments, a BETbromodomain-containing protein is BRD4.

In certain embodiments, the dTAG is derived from, but not limited to,7,8-dihydro-8-oxoguanin triphosphatase, AFAD, Arachidonate5-lipoxygenase activating protein, apolipoprotein, baculoviral IAPrepeat-containing protein 2, Bcl-2, Bcl-xL, E3 ligase XIAP, fatty acidbinding protein from adipocytes 4 (FABP4), GTPase k-RAS, HDAC6,hematopoietic prostaglandin D synthase, lactoglutathione lyase, Mcl-1,PA2GA, peptidyl-prolyl cis-trans isomerase NIMA-interacting 1,poly-ADP-ribose polymerase 14, poly-ADP-ribose polymerase 15,prosaposin, prostaglandin E synthase, retinal rod rhodopsin-sensitivecGMP 3′,′5-cyclic phosphodiesterase subunit delta, S100-A7, Src,Sumo-conjugating enzyme UBC9, superoxide dismutase, tankyrase 1, ortankyrase 2.

In certain embodiments, the dTAG is derived from a nuclear proteinincluding, but not limited to, BRD2, BRD3, BRD4, AntennapediaHomeodomain Protein, BRCA1, BRCA2, CCAAT-Enhanced-Binding Proteins,histones, Polycomb-group proteins, High Mobility Group Proteins,Telomere Binding Proteins, FANCA, FANCD2, FANCE, FANCF, hepatocytenuclear factors, Mad2, NF-kappa B, Nuclear Receptor Coactivators,CREB-binding protein, p55, p107, p130, Rb proteins, p53, c-fos, c-jun,c-mdm2, c-myc, and c-rel.

In a particular embodiment, the dTAG has an amino acid sequence derivedfrom BRD2 ((Universal Protein Resource Knowledge Base (UniProtKB)-P25440(BRD2_HUMAN) incorporated herein by reference), BRD3 (UniProtKB-Q15059(BRD3_HUMAN) incorporated herein by reference), BRD4 (UniProtKB-060885(BRD4_HUMAN) incorporated herein by reference), or BRDT(UniProtKB-Q58F21 (BRDT_HUMAN) incorporated herein by reference) (seeBaud et al., “A bump-and-hole approach to engineer controlledselectivity of BET bromodomains chemical probes”, Science 346(6209)(2014):638-641; and Baud et al., “New Synthetic Routes toTriazolo-benzodiazepine Analogues: Expanding the Scope of theBump-and-Hole Approach for Selective Bromo and Extra-Terminal (BET)Bromodomain Inhibition”, JMC 59 (2016): 1492-1500, both incorporatedherein by reference). In certain embodiments, the dTAG is a modified ormutant BRD2, BRD3, BRD4, or BRDT protein (see Baud et al., “Abump-and-hole approach to engineer controlled selectivity of BETbromodomains chemical probes”, Science 346(6209) (2014):638-641; andBaud et al., “New Synthetic Routes to Triazolo-benzodiazepine Analogues:Expanding the Scope of the Bump-and-Hole Approach for Selective Bromoand Extra-Terminal (BET) Bromodomain Inhibition”, JMC 59(2016):1492-1500, both incorporated herein by reference). In certainembodiments, the one or more mutations of BRD2 include a mutation of theTryptophan (W) at amino acid position 97, a mutation of the Valine (V)at amino acid position 103, a mutation of the Leucine (L) at amino acidposition 110, a mutation of the W at amino acid position 370, a mutationof the V at amino acid position 376, or a mutation of the L at aminoacid position 381. In certain embodiments, the one or more mutations ofBRD3 include a mutation of the W at amino acid position 57, a mutationof the V at amino acid position 63, a mutation of the L at amino acidposition 70, a mutation of the W at amino acid position 332, a mutationof the V at amino acid position 338, or a mutation of the L at aminoacid position 345. In certain embodiments, the one or more mutations ofBRD4 include a mutation of the W at amino acid position 81, a mutationof the V at amino acid position 87, a mutation of the L at amino acidposition 94, a mutation of the W at amino acid position 374, a mutationof the V at amino acid position 380, or a mutation of the L at aminoacid position 387. In certain embodiments, the one or more mutations ofBRDT include a mutation of the W at amino acid position 50, a mutationof the V at amino acid position 56, a mutation of the L at amino acidposition 63, a mutation of the W at amino acid position 293, a mutationof the V at amino acid position 299, or a mutation of the L at aminoacid position 306.

In certain embodiments, the dTAG is derived from a kinase inhibitor, aBET bromodomain-containing protein inhibitor, cytosolic signalingprotein FKBP12 ligand, an HDAC inhibitor, a lysine methyltransferaseinhibitor, an angiogenesis inhibitor, an immunosuppressive compound, andan aryl hydrocarbon receptor (AHR) inhibitor.

In a particular embodiment, the dTAG is derived from cytosolic signalingprotein FKBP12. In certain embodiments, the dTAG is a modified or mutantcytosolic signaling protein FKBP12. In certain embodiments, the modifiedor mutant cytosolic signaling protein FKBP12 contains one or moremutations that create an enlarged binding pocket for FKBP12 ligands. Incertain embodiments, the one or more mutations include a mutation of thephenylalanine (F) at amino acid position 36 to valine (V) (F36V) (ascounted without the methionine start codon) (referred to interchangeablyherein as FKBP* or FKBP12*) (see Clackson et al., “Redesigning anFKBP-ligand interface to generate chemical dimerizers with novelspecificity”, PNAS 95 (1998):10437-10442) (incorporated herein byreference).

In a particular embodiment, the dTAG has an amino acid sequence derivedfrom an FKBP12 protein (UniProtKB-P62942 (FKB1A_HUMAN) incorporatedherein by reference), or variant thereof. In one embodiment, the dTAG isderived from the amino acid sequence: (SEQ. ID. NO.: 1) GVQVETISPGDGRTFPKRG QTCVVHYTGM LEDGKKFDSS RDRNKPFKFM LGKQEVIRGW EEGVAQMSVGQRAKLTISPD YAYGATGHPG IIPPHATLVF DVELLKLE.

In one embodiment, the dTAG is a FKBP12 derived amino acid sequence witha mutation of the phenylalanine (F) at amino acid position 36 (ascounted without the methionine) to valine (V) (F36V) (FKBP*) having theamino acid sequence: (SEQ. ID. NO.: 2)GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLE.

In one embodiment, the dTAG has an amino acid sequence derived from aBRD4 protein (UniProtKB-060885 (BRD4_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom the amino acid sequence:

(SEQ. ID. NO.: 3) MSAESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQPANAASTNPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGTAKPGVSTVPNTTQASTPPQTQTPQPNPPPVQATPFIPFPAVTPDLIVQTPVMTVVPPQPLQTPPPVPPQPQPPPAPAPQPVQSHPPIIAATPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLPPEPKTTKLGQRRESSRPVKPPKKDVPDSQQHPAPEKSSKVSEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLEAREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVAVSSPAVPPPTKVVAPPSSSDSSSDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKRKEEVEENKKSKAKEPPPKKTKKNNSSNSNVSKKEPAPMKSKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESSSESSSSDSEDSETEMAPKSKKKGHPGREQKKHHHHHHQQMQQAPAPVPQQPPPPPQQPPPPPPPQQQQQPPPPPPPPSMPQQAAPAMKSSPPPFIATQWVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPARPPAVSPALTQTPLLPQPPMAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQLQQQPPPPPPPQPQPPPQQQHQPPPRPVHLQPMQFSTHIQQPPPPQGQQPPHPPPGQQPPPPQPAKPQQVIQHHHSPRHHKSDPYSTGHLREAPSPLMIHSPQMSQFQSLTHQSPPQQNVQPKKQELRAASVVQPQPLVVVKEEKIHSPIIRSEPFSPSLRPEPPKHPESIKAPVHLPQRPEMKPVDVGRPVIRPPEQNAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSSSDSFEQFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQRQEQQQQQQQQAAAVAAAATPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQ SDLLSIFEENLF.

In one embodiment, the dTAG is derived from amino acid 75-147 of SEQ.ID. NO.: 3.

In one embodiment, the dTAG has an amino acid sequence derived from aASH1L protein (UniProtKB-Q9NR48 (ASH1L_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 2463-2533 of Q9NR48.

In one embodiment, the dTAG has an amino acid sequence derived from aATAD2 protein (UniProtKB-Q6PL 18 (ATAD2_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 1001-1071 of Q6PL18.

In one embodiment, the dTAG has an amino acid sequence derived from aBAZ1A protein (UniProtKB-Q9NRL2 (BAZ1A_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 1446-1516 of Q9NRL2.

In one embodiment, the dTAG has an amino acid sequence derived from aBAZ1B protein (UniProtKB-Q9UIG0 (BAZ1B_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 1356-1426 of Q9UIG0.

In one embodiment, the dTAG has an amino acid sequence derived from aBAZ2A protein (UniProtKB-Q9UIF9 (BAZ2A_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 1810-1880 of Q9UIF9.

In one embodiment, the dTAG has an amino acid sequence derived from aBAZ2B protein (UniProtKB-Q9UIF8 (BAZ2B_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 2077-2147 of Q9UIF8.

In one embodiment, the dTAG has an amino acid sequence derived from aBRD1 protein (UniProtKB-095696 (BRD1_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 579-649 of 095696.

In one embodiment, the dTAG has an amino acid sequence derived from aBRD2 protein (UniProtKB-P25440 (BRD2_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom the amino acid sequence:

(SEQ. ID. NO.: 13) MLQNVTPHNKLPGEGNAGLLGLGPEAAAPGKRIRKPSLLYEGFESPTMASVPALQLTPANPPPPEVSNPKKPGRVTNQLQYLHKVVMKALWKHQFAWPFRQPVDAVKLGLPDYHKIIKQPMDMGTIKRRLENNYYWAASECMQDFNTMTTNCYIYNKPTDDIVLMAQTLEKIFLQKVASMPQEEQELVVTIPKNSHKKGAKLAALQGSVTSAHQVPAVSSVSHTALYTPPPEIPTTVLNIPHPSVISSPLLKSLHSAGPPLLAVTAAPPAQPLAKKKGVKRKADTTTPTPTAILAPGSPASPPGSLEPKAARLPPMRRESGRPIKPPRKDLPDSQQQHQSSKKGKLSEQLKHCNGILKELLSKKHAAYAWPFYKPVDASALGLHDYHDIIKHPMDLSTVKRKMENRDYRDAQEFAADVRLMFSNCYKYNPPDHDVVAMARKLQDVFEFRYAKMPDEPLEPGPLPVSTAMPPGLAKSSSESSSEESSSESSSEEEEEEDEEDEEEEESESSDSEEERAHRLAELQEQLRAVEMQLAALSQGPISKPKRKREKKEKKKKRKAEKHRGRAGADEDDKGPRAPRPPQPKKSKKASGSGGGSAALGPSGFGPSGGSGTKLPKKATKTAPPALPTGYDSEEEEESRPMSYDEKRQLSLDINKLPGEKLGRVVHIIQAREPSLRDSNPEEIEIDFETLKPSTLRELERYVLSCLRKKPRKPYTIKKPVGKTKEELALEKKRELEKRLQDVSGQLNSTKKPPKKANEKTESSSAQQVAVSRLSASSSSSDSSSSSSSSSSSDTSDSDS G.

In one embodiment, the dTAG is derived from amino acid 91-163 or 364-436of SEQ. ID. NO.: 13.

In one embodiment, the dTAG has an amino acid sequence derived from aBRD3 protein (UniProtKB-Q15059 (BRD3_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom the amino acid sequence:

(SEQ. ID. NO.: 14) MSTATTVAPAGIPATPGPVNPPPPEVSNPSKPGRKTNQLQYMQNVVVKTLWKHQFAWPFYQPVDAIKLNLPDYHKIIKNPMDMGTIKKRLENNVSASECMQDFNTMFTNCYIYNKPTDDIVLMAQALEKIFLQKVAQMPQEEVELLPPAPKGKGRKPAAGAQSAGTQQVAAVSSVSPATPFQSVPPTVSQTPVIAATPVPTITANVTSVPVPPAAAPPPPATPIVPVVPPTPPVVKKKGVKRKADTTTPTTSAITASRSESPPPLSDPKQAKVVARRESGGRPIKPPKKDLEDGEVPQHAGKKGKLSEHLRYCDSILREMLSKKHAAYAWPFYKPVDAEALELEIDYHDIIKHPMDLSTVKRKMDGREYPDAQGFAADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPVEAPALPAPAAPMVSKGAESSRSSEESSSDSGSSDSEEERATRLAELQEQLKAVHEQLAALSQAPVNKPKKKKEKKEKEKKKKDKEKEKEKHKVKAEEEKKAKVAPPAKQAQQKKAPAKKANSTTTAGRQLKKGGKQASASYDSEEEEEGLPMSYDEKRQLSLDINRLPGEKLGRVVHIIQSREPSLRDSNPDEIEIDFETLKPTTLRELERYVKSCLQKKQRKPFSASGKKQAAKSKEELAQEKKKELEKRLQDVSGQLSSSKKPARKEKPGSAPSGGPSRLSSSSSSESGSSSSSGSSSDSSDSE.

In one embodiment, the dTAG is derived from amino acid 51-123 or 326-398of SEQ. ID. NO.: 14.

In one embodiment, the dTAG has an amino acid sequence derived from aBRD7 protein (UniProtKB-Q9NPI1 (BRD7_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 148-218 of Q9NPI1.

In one embodiment, the dTAG has an amino acid sequence derived from aBRD8 protein (UniProtKB-Q9HOE9 (BRD8_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 724-794 or 1120-1190 of Q9HOE9.

In one embodiment, the dTAG has an amino acid sequence derived from aBRD9 protein (UniProtKB-Q9H8M2 (BRD9_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 153-223 of Q9H8M2.

In one embodiment, the dTAG has an amino acid sequence derived from aBRDT protein (UniProtKB-Q58F21 (BRDT_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom the amino acid sequence:

(SEQ. ID. NO.: 15) MSLPSRQTAIIVNPPPPEYINTKKNGRLTNQLQYLQKVVLKDLWKHSFSWPFQRPVDAVKLQLPDYYTIIKNPMDLNTIKKRLENKYYAKASECIEDFNTMFSNCYLYNKPGDDIVLMAQALEKLFMQKLSQMPQEEQVVGVKERIKKGTQQNIAVSSAKEKSSPSATEKVFKQQEIPSVFPKTSISPLNVVQGASVNSSSQTAAQVTKGVKRKADTTTPATSAVKASSEFSPTFTEKSVALPPIKENMPKNVLPDSQQQYNVVKTVKVTEQLRHCSEILKEMLAKKHFSYAWPFYNPVDVNALGLHNYYDVVKNPMDLGTIKEKMDNQEYKDAYKFAADVRLMFMNCYKYNPPDHEVVTMARMLQDVFETHFSKIPIEPVESMPLCYIKTDITETTGRENTNEASSEGNSSDDSEDERVKRLAKLQEQLKAVHQQLQVLSQVPFRKLNKKKEKSKKEKKKEKVNNSNENPRKMCEQMRLKEKSKRNQPKKRKQQFIGLKSEDEDNAKPMNYDEKRQLSLNINKLPGDKLGRVVHIIQSREPSLSNSNPDEIEIDFETLKASTLRELEKYVSACLRKRPLKPPAKKIMINISKEELHSQKKQELEKRLLDVNNQLNSRKRQTKSDKTQPSKAVENVSRLSESSSSSSSSSESESSSSDLSSSDSSDSESEMFPKFTEVKPNDSPSKENVKKMKNECIPPEGRTGVTQIGYCVQDTTSANTTLVHQTTPSHVMPPNHHQLAFNYQELEHLQTVKNISPLQILPPSGDSEQLSNGITVMHPSGDSDTTMLESECQAPVQKDIKIKNADSWKSLGKPVKPSGVMKSSDELFNQFRKAAIEKEVKARTQELIRKHLEQNTKELKASQENQRDLGNGLTVESFSNKIQNKCSGEEQKEHQQSSEAQDKSKLWLLKDRDLARQKEQERRRREAMVGTIDMTLQSDIMTMFENNFD.

In one embodiment, the dTAG is derived from amino acid 44-116 or 287-359of SEQ. ID. NO.: 15.

In one embodiment, the dTAG has an amino acid sequence derived from aBRPF1 protein (UniProtKB-P55201 (BRPF1_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 645-715 of P55201.

In one embodiment, the dTAG has an amino acid sequence derived from aBRPF3 protein (UniProtKB-Q9ULD4 (BRPF3_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 606-676 of Q9ULD4.

In one embodiment, the dTAG has an amino acid sequence derived from aBRWD3 protein (UniProtKB-Q6RI45 (BRWD3_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 1158-1228 or 1317-1412 of Q6RI45.

In one embodiment, the dTAG has an amino acid sequence derived from aCECR2 protein (UniProtKB-Q9BXF3 (CECR2_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 451-521 of Q9BXF3.

In one embodiment, the dTAG has an amino acid sequence derived from aCREBBP protein (UniProtKB-Q92793 (CBP_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 1103-1175 of Q92793.

In one embodiment, the dTAG has an amino acid sequence derived from aEP300 protein (UniProtKB-Q09472 (EP300_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 1067-1139 of Q09472.

In one embodiment, the dTAG has an amino acid sequence derived from aFALZ protein (UniProtKB-Q12830 (BPTF_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 2944-3014 of Q12830.

In one embodiment, the dTAG has an amino acid sequence derived from aGCN5L2 protein (UniProtKB-Q92830 (KAT2A_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 745-815 of Q92830.

In one embodiment, the dTAG has an amino acid sequence derived from aKIAA1240 protein (UniProtKB-Q9ULI0 (ATD2B_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 975-1045 of Q9ULI0.

In one embodiment, the dTAG has an amino acid sequence derived from aLOC93349 protein (UniProtKB-Q13342 (SP140_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 796-829 of Q13342.

In one embodiment, the dTAG has an amino acid sequence derived from aMLL protein (UniProtKB-Q03164 (KMT2A_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 1703-1748 of Q03164.

In one embodiment, the dTAG has an amino acid sequence derived from aPB1 protein (UniProtKB-Q86U86 (PB_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 63-134, 200-270, 400-470, 538-608, 676-746, or 792-862of Q86U86.

In one embodiment, the dTAG has an amino acid sequence derived from aPCAF protein (UniProtKB-Q92831 (KAT2B_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 740-810 of Q92831.

In one embodiment, the dTAG has an amino acid sequence derived from aPHIP protein (UniProtKB-Q8WWQ0 (PHIP_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 1176-1246 or 1333-1403 of Q8WWQ0.

In one embodiment, the dTAG has an amino acid sequence derived from aPRKCBP1 protein (UniProtKB-Q9ULU4 (PKCB1_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 165-235 of Q9ULU4.

In one embodiment, the dTAG has an amino acid sequence derived from aSMARCA2 protein (UniProtKB-P51531 (SMCA2_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 1419-1489 of P51531.

In one embodiment, the dTAG has an amino acid sequence derived from aSMARCA4 protein (UniProtKB-P51532 (SMCA4_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 1477-1547 of P51532.

In one embodiment, the dTAG has an amino acid sequence derived from aSP100 protein (UniProtKB-P23497 (SP100_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 761-876 of P23497.

In one embodiment, the dTAG has an amino acid sequence derived from aSP110 protein (UniProtKB-Q9HB58 (SP110_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 581-676 of Q9HB58.

In one embodiment, the dTAG has an amino acid sequence derived from aSP140 protein (UniProtKB-Q13342 (SP140_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 796-829 of Q13342.

In one embodiment, the dTAG has an amino acid sequence derived from aTAF1 protein (UniProtKB-P21675 (TAF1_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 1397-1467 or 1520-1590 of P21675.

In one embodiment, the dTAG has an amino acid sequence derived from aTAF1L protein (UniProtKB-Q8IZX4 (TAF1L_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 1416-1486 or 1539-1609 of Q8IZX4.

In one embodiment, the dTAG has an amino acid sequence derived from aTIF1A protein (UniProtKB-O15164 (TIF1A_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 932-987 of O15164.

In one embodiment, the dTAG has an amino acid sequence derived from aTRIM28 protein (UniProtKB-Q13263 (TIF1B_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 697-801 of Q13263.

In one embodiment, the dTAG has an amino acid sequence derived from aTRIM33 protein (UniProtKB-Q9UPN9 (TRI33_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 974-1046 of Q9UPN9.

In one embodiment, the dTAG has an amino acid sequence derived from aTRIM66 protein (UniProtKB-015016 (TRI66_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 1056-1128 of O15016.

In one embodiment, the dTAG has an amino acid sequence derived from aWDR9 protein (UniProtKB-Q9NSI6 (BRWD1_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 1177-1247 or 1330-1400 of Q9NSI6.

In one embodiment, the dTAG has an amino acid sequence derived from aZMYND11 protein (UniProtKB-Q15326 (ZMY1_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 168-238 of Q15326.

In one embodiment, the dTAG has an amino acid sequence derived from aMLL4 protein (UniProtKB-Q9UMN6 (KMT2B_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 1395-1509 of Q9UMN6.

In one embodiment, the dTAG has an amino acid sequence derived from anestrogen receptor, human (UniProtKB-P03372-1) (incorporated herein byreference), or a variant thereof. In one embodiment, the dTAG is derivedfrom the amino acid sequence:

(SEQ. ID. NO.: 4) MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV.

In one embodiment, the dTAG has an amino acid sequence derived from anestrogen receptor ligand-binding domain, or a variant thereof. In oneembodiment, the dTAG is derived from the amino acid sequence:

(SEQ. ID. NO.: 5) SLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHIVISNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRL.

In one embodiment, the dTAG has an amino acid sequence derived from anandrogen receptor, UniProtKB-P10275 (ANDR_HUMAN) (incorporated herein byreference), or a variant thereof. In one embodiment, the dTAG is derivedfrom the amino acid sequence:

(SEQ. ID. NO.: 6) MEVQLGLGRVYPRPPSKTYRGAFQNLFQSVREVIQNPGPRHPEAASAAPPGASLLLLQQQQQQQQQQQQQQQQQQQQQQQETSPRQQQQQQGEDGSPQAHRRGPTGYLVLDEEQQPSQPQSALECHPERGCVPEPGAAVAASKGLPQQLPAPPDEDDSAAPSTLSLLGPTFPGLSSCSADLKDILSEASTMQLLQQQQQEAVSEGSSSGRAREASGAPTSSKDNYLGGTSTISDNAKELCKAVSVSMGLGVEALEHLSPGEQLRGDCMYAPLLGVPPAVRPTPCAPLAECKGSLLDDSAGKSTEDTAEYSPFKGGYTKGLEGESLGCSGSAAAGSSGTLELPSTLSLYKSGALDEAAAYQSRDYYNFPLALAGPPPPPPPPHPHARIKLENPLDYGSAWAAAAAQCRYGDLASLHGAGAAGPGSGSPSAAASSSWHTLFTAEEGQLYGPCGGGGGGGGGGGGGGGGGGGGGGGEAGAVAPYGYTRPPQGLAGQESDFTAPDVWYPGGMVSRVPYPSPTCVKSEMGPWMDSYSGPYGDMRLETARDHVLPIDYYFPPQKTCLICGDEASGCHYGALTCGSCKVFFKRAAEGKQKYLCASRNDCTIDKFRRKNCPSCRLRKCYEAGMTLGARKLKKLGNLKLQEEGEASSTTSPTEETTQKLTVSHIEGYECQPIFLNVLEAIEPGVVCAGHDNNQPDSFAALLSSLNELGERQLVHVVKWAKALPGFRNLHVDDQMAVIQYSWMGLMVFAMGWRSFTNVNSRMLYFAPDLVFNEYRMHKSRMYSQCVRMRHLSQEFGWLQITPQEFLCMKALLLFSIIPVDGLKNQKFFDELRMNYIKELDRIIACKRKNPTSCSRRFYQLTKLLDSVQPIARELHQFTFDLLIKSHMVSVDFPEMMAEII SVQVPKILSGKVKPIYFHTQ.

In one embodiment, the dTAG has an amino acid sequence derived from anandrogen receptor ligand-binding domain, or a variant thereof. In oneembodiment, the dTAG is derived from the amino acid sequence:

(SEQ. ID. NO.: 10) DNNQPDSFAALLSSLNELGERQLVHVVKWAKALPGFRNLHVDDQMAVIQYSWMGLMVFAMGWRSFTNVNSRMLYFAPDLVFNEYRMHKSRMYSQCVRMRHLSQEFGWLQITPQEFLCMKALLLFSIIPVDGLKNQKFFDELRMNYIKELDRIIACKRKNPTSCSRRFYQLTKLLDSVQPIARELHQFTFDLLIKSHMVSVDFPEMMAEIISVQVPKILSGKVKPIYFHT.

In one embodiment, the dTAG has an amino acid sequence derived from aRetinoic Receptor, (UniProtKB-P19793) (RXRA_HUMAN) (incorporated hereinby reference), or a variant thereof. In one embodiment, the dTAG isderived from the amino acid sequence:

(SEQ. ID. NO.: 7) MDTKHFLPLDFSTQVNSSLTSPTGRGSMAAPSLHPSLGPGIGSPGQLHSPISTLSSPINGMGPPFSVISSPMGPHSMSVPTTPTLGFSTGSPQLSSPMNPVSSSEDIKPPLGLNGVLKVPAHPSGNMASFTKHICAICGDRSSGKHYGVYSCEGCKGFFKRTVRKDLTYTCRDNKDCLIDKRQRNRCQYCRYQKCLAMGMKREAVQEERQRGKDRNENEVESTSSANEDMPVERILEAELAVEPKTETYVEANMGLNPSSPNDPVTNICQAADKQLFTLVEWAKRIPHFSELPLDDQVILLRAGWNELLIASFSHRSIAVKDGILLATGLHVHRNSAHSAGVGAIFDRVLTELVSKMRDMQMDKTELGCLRAIVLFNPDSKGLSNPAEVEALREKVYASLEAYCKHKYPEQPGRFAKLLLRLPALRSIGLKCLEHLFFFKLIGDTPIDTFLMEMLEAPHQMT.

In one embodiment, the dTAG has an amino acid sequence derived from aRetinoic Receptor ligand-binding domain, or a variant thereof. In oneembodiment, the dTAG is derived from the amino acid sequence:

(SEQ. ID. NO.: 11) SANEDMPVERILEAELAVEPKTETYVEANMGLNPSSPNDPVTNICQAADKQLFTLVEWAKRIPHFSELPLDDQVILLRAGWNELLIASFSHRSIAVKDGILLATGLHVHRNSAHSAGVGAIFDRVLTELVSKMRDMQMDKTELGCLRAIVLFNPDSKGLSNPAEVEALREKVYASLEAYCKHKYPEQPGRFAKLLLRLPALRSIGLKCLEHLFFFKLIGDTPIDTFLMEMLEAPHQMT.

In one embodiment, the dTAG has an amino acid sequence derived from aDHFR, E. coli, UniProtKB-Q79DQ2 (Q79DQ2_ECOLX) (incorporated herein byreference), or a variant thereof. In one embodiment, the dTAG is derivedfrom the amino acid sequence:

(SEQ. ID. NO.: 8) MNSESVRIYLVAAMGANRVIGNGPNIPWKIPGEQKIFRRLTEGKVVVMGRKTFESIGKPLPNRHTLVISRQANYRATGCVVVSTLSHAIALASELGNELYVAGGAEIYTLALPHAHGVFLSEVHQTFEGDAFFPMLNETEFELV STETIQAVIPYTHSVYARRNG.

In one embodiment, the dTAG has an amino acid sequence derived from abacterial dehalogenase, or variant thereof. In one embodiment, the dTAGis derived from the amino acid sequence:

(SEQ. ID. NO.: 9) MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIAR WLSTLEISG.

In one embodiment, the dTAG has an amino acid sequence derived from theN-terminus of MDM2, or variants thereof. In one embodiment, the dTAG isderived from the amino acid sequence:

(SEQ. ID. NO.: 12) MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEH RKIYTMIYRNLVVV.

In one embodiment, the dTAG has an amino acid sequence derived fromapoptosis regulator Bcl-xL protein, UniProtKB-Q07817 (B2CL1_HUMAN)(incorporated herein by reference), or a variant thereof. In oneembodiment, the dTAG is derived from the amino acid sequence:

(SEQ. ID. NO.: 16) MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIAAWMATYLNDHLEPWIQENGGWDTFVELYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK.

In one embodiment, the dTAG has an amino acid sequence derived from theCD209 antigen, UniProtKB-Q9NNX6 (CD209_HUMAN) (incorporated herein byreference), or a variant thereof. In one embodiment, the dTAG is derivedfrom the amino acid sequence:

(SEQ. ID. NO.: 17) MSDSKEPRLQQLGLLEEEQLRGLGFRQTRGYKSLAGCLGHGPLVLQLLSFTLLAGLLVQVSKVPSSISQEQSRQDAIYQNLTQLKAAVGELSEKSKLQEIYQELTQLKAAVGELPEKSKLQEIYQELTRLKAAVGELPEKSKLQEIYQELTWLKAAVGELPEKSKMQEIYQELTRLKAAVGELPEKSKQQEIYQELTRLKAAVGELPEKSKQQEIYQELTRLKAAVGELPEKSKQQEIYQELTQLKAAVERLCHPCPWEWTFFQGNCYFMSNSQRNWHDSITACKEVGAQLVVIKSAEEQNFLQLQSSRSNRFTWMGLSDLNQEGTWQWVDGSPLLPSFKQYWNRGEPNNVGEEDCAEFSGNGWNDDKCNLAKFWICKKSAASC SRDEEQFLSPAPATPNPPPA.

In one embodiment, the dTAG has an amino acid sequence derived from E3ligase XIAP, UniProtKB-P98170 (XIAP_HUMAN) (incorporated herein byreference), or a variant thereof. In one embodiment, the dTAG is derivedfrom the amino acid sequence:

(SEQ. ID. NO.: 18) MTFNSFEGSKTCVPADINKEEEFVEEFNRLKTFANFPSGSPVSASTLARAGFLYTGEGDTVRCFSCHAAVDRWQYGDSAVGRHRKVSPNCRFINGFYLENSATQSTNSGIQNGQYKVENYLGSRDHFALDRPSETHADYLLRTGQVVDISDTIYPRNPAMYSEEARLKSFQNWPDYAHLTPRELASAGLYYTGIGDQVQCFCCGGKLKNWEPCDRAWSEHRRHFPNCFFVLGRNLNIRSESDAVSSDRNFPNSTNLPRNPSMADYEARIFTFGTWIYSVNKEQLARAGFYALGEGDKVKCFHCGGGLTDWKPSEDPWEQHAKWYPGCKYLLEQKGQEYINNIHLTHSLEECLVRTTEKTPSLTRRIDDTIFQNPMVQEAIRMGFSFKDIKKIMEEKIQISGSNYKSLEVLVADLVNAQKDSMQDESSQTSLQKEISTEEQLRRLQEEKLCKICMDRNIAIVFVPCGHLVTCKQCAEAVDK CPMCYTVITFKQKIFMS.

In one embodiment, the dTAG has an amino acid sequence derived frombaculoviral IAP repeat-containing protein 2, UniProtKB-Q13490(BIRC2_HUMAN) (incorporated herein by reference) or a variant thereof.In one embodiment, the dTAG is derived from the amino acid sequence:

(SEQ. ID. NO.: 19) MHKTASQRLFPGPSYQNIKSIMEDSTILSDWTNSNKQKMKYDFSCELYRMSTYSTFPAGVPVSERSLARAGFYYTGVNDKVKCFCCGLMLDNWKLGDSPIQKEIKQLYPSCSFIQNLVSASLGSTSKNTSPMRNSFAHSLSPTLEHSSLFSGSYSSLSPNPLNSRAVEDISSSRTNPYSYAMSTEEARFLTYHMWPLTFLSPSELARAGFYYIGPGDRVACFACGGKLSNWEPKDDAMSEHRRHFPNCPFLENSLETLRFSISNLSMQTHAARMRTFMYWPSSVPVQPEQLASAGFYYVGRNDDVKCFCCDGGLRCWESGDDPWVEHAKWFPRCEFLIRMKGQEFVDEIQGRYPHLLEQLLSTSDTTGEENADPPIIHFGPGESSSEDAVMMNTPVVKSALEMGFNRDLVKQTVQSKILTTGENYKTVNDIVSALLNAEDEKREEEKEKQAEEMASDDLSLIRKNRMALFQQLTCVLPILDNLLKANVINKQEHDIIKQKTQIPLQARELIDTILVKGNAAANIFKNCLKEIDSTLYKNLFVDKNMKYIPTEDVSGLSLEEQLRRLQEERTCKVCMDKEVSVVFIPCGHLVVCQECAPSLRKCPICRGIIKGTVRTFLS.

In one embodiment, the dTAG has an amino acid sequence derived fromhematopoietic prostaglandin D synthase, UniProtKB-O60760 (HPGDS_HUMAN)(incorporated herein by reference), or a variant thereof. In oneembodiment, the dTAG is derived from the amino acid sequence:

(SEQ. ID. NO.: 20) MPNYKLTYFNMRGRAEIIRYIFAYLDIQYEDHRIEQADWPEIKSTLPFGKIPILEVDGLTLHQSLAIARYLTKNTDLAGNTEMEQCHVDAIVDTLDDFMSCFPWAEKKQDVKEQMFNELLTYNAPHLMQDLDTYLGGREWLIGNSVTWADFYWEICSTTLLVFKPDLLDNHPRLVTLRKKVQAIPAVANWIK RRPQTKL.

In one embodiment, the dTAG has an amino acid sequence derived fromGTPase k-RAS, UniProtKB-P01116 (RASK_HUMAN) (incorporated herein byreference), or a variant thereof. In one embodiment, the dTAG is derivedfrom the amino acid sequence:

(SEQ. ID. NO.: 21) MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQRVEDAFYTLVREIRQYRLKKISKEEKTPGCVKIKKCIIM.

In one embodiment, the dTAG has an amino acid sequence derived fromPoly-ADP-ribose polymerase 15, UniProtKB-Q460N3 (PAR15_HUMAN)(incorporated herein by reference), or a variant thereof. In oneembodiment, the dTAG is derived from the amino acid sequence:

(SEQ. ID. NO.: 22) MAAPGPLPAAALSPGAPTPRELMHGVAGVTSRAGRDREAGSVLPAGNRGARKASRRSSSRSMSRDNKFSKKDCLSIRNVVASIQTKEGLNLKLISGDVLYIWADVIVNSVPMNLQLGGGPLSRAFLQKAGPMLQKELDDRRRETEEKVGNIFMTSGCNLDCKAVLHAVAPYWNNGAETSWQIMANIIKKCLTTVEVLSFSSITFPMIGTGSLQFPKAVFAKLILSEVFEYSSSTRPITSPLQEVHFLVYTNDDEGCQAFLDEFTNWSRINPNKARIPMAGDTQGVVGTVSKPCFTAYEMKIGAITFQVATGDIATEQVDVIVNSTARTFNRKSGVSRAILEGAGQAVESECAVLAAQPHRDFIITPGGCLKCKIIIHVPGGKDVRKTVTSVLEECEQRKYTSVSLPAIGTGNAGKNPITVADNIIDAIVDFSSQHSTPSLKTVKVVIFQPELLNIFYDSMKKRDLSASLNFQSTFSMTTCNLPEHWTDMNHQLFCMVQLEPGQSEYNTIKDKFTRTCSSYAIEKIERIQNAFLWQSYQVKKRQMDIKNDHKNNERLLFHGTDADSVPYVNQHGFNRSCAGKNAVSYGKGTYFAVDASYSAKDTYSKPDSNGRKHMYVVRVLTGVFTKGRAGLVTPPPKNPHNPTDLFDSVTNNTRSPKLFVVFFDNQAYPEY LITFTA.

In one embodiment, the dTAG has an amino acid sequence derived fromPoly-ADP-ribose polymerase 14, UniProtKB Q460N5 (PAR14_HUMAN)(incorporated herein by reference), or a variant thereof. In oneembodiment, the dTAG is derived from the amino acid sequence:

(SEQ. ID. NO.: 23) MAVPGSFPLLVEGSWGPDPPKNLNTKLQMYFQSPKRSGGGECEVRQDPRSPSRFLVFFYPEDVRQKVLERKNHELVWQGKGTFKLTVQLPATPDEIDHVFEEELLTKESKTKEDVKEPDVSEELDTKLPLDGGLDKMEDIPEECENISSLVAFENLKANVTDIMLILLVENISGLSNDDFQVEIIRDFDVAVVTFQKHIDTIRFVDDCTKHHSIKQLQLSPRLLEVTNTIRVENLPPGADDYSLKLFFENPYNGGGRVANVEYFPEESSALIEFFDRKVLDTIMATKLDFNKMPLSVFPYYASLGTALYGKEKPLIKLPAPFEESLDLPLWKFLQKKNHLIEEINDEMRRCHCELTWSQLSGKVTIRPAATLVNEGRPRIKTWQADTSTTLSSIRSKYKVNPIKVDPTMWDTIKNDVKDDRILIEFDTLKEMVILAGKSEDVQSIEVQVRELIESTTQKIKREEQSLKEKMIISPGRYFLLCHSSLLDHLLTECPEIEICYDRVTQHLCLKGPSADVYKAKCEIQEKVYTMAQKNIQVSPEIFQFLQQVNWKEFSKCLFIAQKILALYELEGTTVLLTSCSSEALLEAEKQMLSALNYKRIEVENKEVLHGKKWKGLTHNLLKKQNSSPNTVIINELTSETTAEVIITGCVKEVNETYKLLFNFVEQNMKIERLVEVKPSLVIDYLKTEKKLFWPKIKKVNVQVSFNPENKQKGILLTGSKTEVLKAVDIVKQVWDSVCVKSVHTDKPGAKQFFQDKARFYQSEIKRLFGCYIELQENEVMKEGGSPAGQKCFSRTVLAPGVVLIVQQGDLARLPVDVVVNASNEDLKHYGGLAAALSKAAGPELQADCDQIVKREGRLLPGNATISKAGKLPYHHVIHAVGPRWSGYEAPRCVYLLRRAVQLSLCLAEKYKYRSIAIPAISSGVFGFPLGRCVETIVSAIKENFQFKKDGHCLKEIYLVDVSEKTVEAFAEAVKTVFKATLPDTAAPPGLPPAAAGPGKTSWEKGSLVSPGGLQMLLVKEGVQNAKTDVVVNSVPLDLVLSRGPLSKSLLEKAGPELQEELDTVGQGVAVSMGTVLKTSSWNLDCRYVLHVVAPEWRNGSTSSLKIMEDIIRECMEITESLSLKSIAFPAIGTGNLGFPKNIFAELIISEVFKFSSKNQLKTLQEVHFLLHPSDHENIQAFSDEFARRANGNLVSDKIPKAKDTQGFYGTVSSPDSGVYEMKIGSIIFQVASGDITKEEADVIVNSTSNSFNLKAGVSKAILECAGQNVERECSQQAQQRKNDYIITGGGFLRCKNIIHVIGGNDVKSSVSSVLQECEKKNYSSICLPAIGTGNAKQHPDKVAEAIIDAIEDFVQKGSAQSVKKVKVVIFLPQVLDVFYANMKKREGTQLSSQQSVMSKLASFLGFSKQSPQKKNHLVLEKKTESATFRVCGENVTCVEYAISWLQDLIEKEQCPYTSEDECIKDFDEKEYQELNELQKKLNINISLDHKRPLIKVLGISRDVMQARDEIEAMIKRVRLAKEQESRADCISEFIEWQYNDNNTSHCFNKMTNLKLEDARREKKKTVDVKINHRHYTVNLNTYTATDTKGHSLSVQRLTKSKVDIPAHWSDMKQQNFCVVELLPSDPEYNTVASKFNQTCSHFRIEKIERIQNPDLWNSYQAKKKTMDAKNGQTMNEKQLFHGTDAGSVPHVNRNGFNRSYAGKNAVAYGKGTYFAVNANYSANDTYSRPDANGRKHVYYVRVLTGIYTHGNHSLIVPPSKNPQNPTDLYDTVTDNVHHPSLFVAFYDYQAYPEYLITFRK.

In one embodiment, the dTAG has an amino acid sequence derived fromsuperoxide dismutase, UniProtKB-P00441 (SODC_HUMAN) (incorporated hereinby reference), or a variant thereof. In one embodiment, the dTAG isderived from the amino acid sequence:

(SEQ. ID. NO.: 24) MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSR LACGVIGIAQ.

In one embodiment, the dTAG has an amino acid sequence derived fromretinal rod rhodopsin-sensitive cGMP 3′,5′-cyclic phosphodiesterasesubunit delta, UniProtKB-043924 (PDE6D HUMAN) (incorporated herein byreference), or a variant thereof. In one embodiment, the dTAG is derivedfrom the amino acid sequence:

(SEQ. ID. NO.: 25) MSAKDERAREILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVSRELNFSSTEQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPASVLTGNVIIETKFFDDDLLVSTSRVRLFYV.

In one embodiment, the dTAG has an amino acid sequence derived frominduced myeloid leukemia cell differentiation protein Mcl-1,UniProtKB-Q07820 (MCL1_HUMAN) (incorporated herein by reference), or avariant thereof. In one embodiment, the dTAG is derived from the aminoacid sequence:

(SEQ. ID. NO.: 26) MFGLKRNAVIGLNLYCGGAGLGAGSGGATRPGGRLLATEKEASARREIGGGEAGAVIGGSAGASPPSTLTPDSRRVARPPPIGAEVPDVTATPARLLFFAPTRRAAPLEEMEAPAADAIMSPEEELDGYEPEPLGKRPAVLPLLELVGESGNNTSTDGSLPSTPPPAEEEEDELYRQSLEIISRYLREQATGAKDTKPMGRSGATSRKALETLRRVGDGVQRNEHETAFQGMLRKLDIKNEDDVKSLSRVMIHVFSDGVTNWGRIVTLISFGAFVAKHLKTINQESCIEPLAESITDVLVRTKRDWLVKQRGWDGFVEFFHVEDLEGGIRNVLLAFAGVAGVGAGLAYL IR.

In one embodiment, the dTAG has an amino acid sequence derived fromapoptosis regulator Bcl-2, UniProtKB-Q07820 (BCL2_HUMAN) (incorporatedherein by reference), or a variant thereof. In one embodiment, the dTAGis derived from the amino acid sequence:

(SEQ. ID. NO. : 27) MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAPAPGIFSSQPGHTPHPAASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDNIALWMTEYLNRHLHTWIQDNGGWDAFVELYGPSMRPLFDFSWLSLKTLLSLALVGACITLGAYLGHK.

In one embodiment, the dTAG has an amino acid sequence derived frompeptidyl-prolyl cis-trans isomerase NIMA-interacting 1, UniProtKB-Q13526(PIN1_HUMAN) (incorporated herein by reference), or a variant thereof.In one embodiment, the dTAG is derived from the amino acid sequence:

(SEQ. ID. NO.: 28) MADEEKLPPGWEKRMSRSSGRVYYFNHITNASQWERPSGNSSSGGKNGQGEPARVRCSHLLVKHSQSRRPSSWRQEKITRTKEEALELINGYIQKIKSGEEDFESLASQFSDCSSAKARGDLGAFSRGQMQKPFEDASFALRTGEMSGPV FTDSGIHIILRTE.

In one embodiment, the dTAG has an amino acid sequence derived fromtankyrase 1, UniProtKB-095271 (TNKS1_HUMAN) (incorporated herein byreference), or a variant thereof. In one embodiment, the dTAG is derivedfrom the amino acid sequence:

(SEQ. ID. NO.: 29) MAASRRSQHHHHHHQQQLQPAPGASAPPPPPPPPLSPGLAPGTTPASPTASGLAPFASPRHGLALPEGDGSRDPPDRPRSPDPVDGTSCCSTTSTICTVAAAPVVPAVSTSSAAGVAPNPAGSGSNNSPSSSSSPTSSSSSSPSSPGSSLAESPEAAGVSSTAPLGPGAAGPGTGVPAVSGALRELLEACRNGDVSRVKRLVDAANVNAKDMAGRKSSPLHFAAGFGRKDVVEHLLQMGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAKAVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLVNCHGKSAVDMAPTPELRERLTYEFKGHSLLQAAREADLAKVKKTLALEIINFKQPQSHETALHCAVASLHPKRKQVTELLLRKGANVNEKNKDFMTPLHVAAERAHNDVMEVLHKHGAKMNALDTLGQTALHRAALAGHLQTCRLLLSYGSDPSIISLQGFTAAQMGNEAVQQILSESTPIRTSDVDYRLLEASKAGDLETVKQLCSSQNVNCRDLEGRHSTPLHFAAGYNRVSVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPTKKNRDGNTPLDLVKEGDTDIQDLLRGDAALLDAAKKGCLARVQKLCTPENINCRDTQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTPLDLATADDIRALLIDAMPPEALPTCFKPQATVVSASLISPASTPSCLSAASSIDNLTGPLAELAVGGASNAGDGAAGTERKEGEVAGLDMNISQFLKSLGLEHLRDIFETEQITLDVLADMGHEELKEIGINAYGHRHKLIKGVERLLGGQQGTNPYLTFHCVNQGTILLDLAPEDKEYQSVEEEMQSTIREHRDGGNAGGIFNRYNVIRIQKVVNKKLRERFCHRQKEVSEENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYICHRQMLFCRVTLGKSFLQFSTMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQIMKPEAPSQTATAAEQKT.

In one embodiment, the dTAG has an amino acid sequence derived fromtankyrase 2, UniProtKB-09H2K2 (TNKS2_HUMAN) (incorporated herein byreference), or a variant thereof. In one embodiment, the dTAG is derivedfrom the amino acid sequence:

(SEQ. ID. NO.: 30) MSGRRCAGGGAACASAAAEAVEPAARELFEACRNGDVERVKRLVTPEKVNSRDTAGRKSTPLHFAAGFGRKDVVEYLLQNGANVQARDDGGLIPLHNACSFGHAEVVNLLLRHGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGAEPTIRNTDGRTALDLADPSAKAVLTGEYKKDELLESARSGNEEKMMALLTPLNVNCHASDGRKSTPLHLAAGYNRVKIVQLLLQHGADVHAKDKGDLVPLHNACSYGHYEVTELLVKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSYGADPTLLNCHNKSAIDLAPTPQLKERLAYEFKGHSLLQAAREADVTRIKKHLSLEMVNFKHPQTHETALHCAAASPYPKRKQICELLLRKGANINEKTKEFLTPLHVASEKAHNDVVEVVVKHEAKVNALDNLGQTSLHRAAYCGHLQTCRLLLSYGCDPNIISLQGFTALQMGNENVQQLLQEGISLGNSEADRQLLEAAKAGDVETVKKLCTVQSVNCRDIEGRQSTPLHFAAGYNRVSVVEYLLQHGADVHAKDKGGLVPLHNACSYGHYEVAELLVKHGAVVNVADLWKFTPLHEAAAKGKYEICKLLLQHGADPTKKNRDGNTPLDLVKDGDTDIQDLLRGDAALLDAAKKGCLARVKKLSSPDNVNCRDTQGRHSTPLHLAAGYNNLEVAEYLLQHGADVNAQDKGGLIPLHNAASYGHVDVAALLIKYNACVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTLKNQEGQTPLDLVSADDVSALLTAAMPPSALPSCYKPQVLNGVRSPGATADALSSGPSSPSSLSAASSLDNLSGSFSELSSVVSSSGTEGASSLEKKEVPGVDFSITQFVRNLGLEHLMDIFEREQITLDVLVEMGHKELKEIGINAYGHRHKLIKGVERLISGQQGLNPYLTLNTSGSGTILIDLSPDDKEFQSVEEEMQSTVREHRDGGHAGGIFNRYNILKIQKVCNKKLWERYTHRRKEVSEENHNHANERMLFHGSPFVNAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPVHKDRSCYICHRQLLFCRVTLGKSFLQFSAMKMAHSPPGHHSVTGRPSVNGLALAEYVIYRGEQAYPE YLITYQIMRPEGMVDG.

In one embodiment, the dTAG has an amino acid sequence derived from7,8-dihydro-8-oxoguanin triphosphatase, UniProtKB-P36639 (8ODP_HUMAN)(incorporated herein by reference), or a variant thereof. In oneembodiment, the dTAG is derived from the amino acid sequence:

(SEQ. ID. NO.: 31) MYWSNQITRRLGERVQGFMSGISPQQMGEPEGSWSGKNPGTMGASRLYTLVLVLQPQRVLLGMKKRGFGAGRWNGFGGKVQEGETIEDGARRELQEESGLTVDALHKVGQIVFEFVGEPELMDVHVFCTDSIQGTPVESDEMRPCWFQLDQIPFKDMWPDDSWFPLLLQKKKFHGYFKFQGQDTILDYTLREVDTV.

In one embodiment, the dTAG has an amino acid sequence derived fromProto-oncogene tyrosine protein kinase Src, UniProtKB-P12931 (SRC_HUMAN)(incorporated herein by reference), or a variant thereof. In oneembodiment, the dTAG is derived from the amino acid sequence:

(SEQ. ID. NO.: 32) MGSNKSKPKDASQRRRSLEPAENVHGAGGGAFPASQTPSKPASADGHRGPSAAFAPAAAEPKLFGGFNSSDTVTSPQRAGPLAGGVTTFVALYDYESRTETDLSFKKGERLQIVNNTEGDWWLAHSLSTGQTGYIPSNYVAPSDSIQAEEWYFGKITRRESERLLLNAENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFNSLQQLVAYYSKHADGLCHRLTTVCPTSKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMSKGSLLDFLKGETGKYLRLPQLVDMAAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPPECPESLHDLMCQCWRKEPEERPTFEYLQAFLEDYFTSTEPQYQPGENL.

In one embodiment, the dTAG includes a substitution of Threonine (T)with Glycine (G) or Alanine (A) at amino acid position 341. In oneembodiment, the dTAG is an amino acid sequence derived from, or afragment thereof, of SEQ. ID. NO.: 62.

LRLEVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYGSKGSLLDFLKGETGKYLRLPQLVDMAAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPPECPESLHDLMCQCWRKEPEERPTFEYLQAFLEDYF.

In one embodiment, the dTAG is an amino acid sequence derived from, or afragment thereof, of SEQ. ID. NO.: 63.

LRLEVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYASKGSLLDFLKGETGKYLRLPQLVDMAAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPPECPESLHDLMCQCWRKEPEERPTFEYLQAFLEDYF

In one embodiment, the dTAG has an amino acid sequence derived fromprostaglandin E synthase, UniProtKB-014684 (PTGES_HUMAN) (incorporatedherein by reference), or a variant thereof. In one embodiment, the dTAGis derived from the amino acid sequence:

(SEQ. ID. NO.: 33) MPAHSLVMSSPALPAFLLCSTLLVIKMYVVAIITGQVRLRKKAFANPEDALRHGGPQYCRSDPDVERCLRAHRNDMETIYPFLFLGFVYSFLGPNPFVAWMHFLVFLVGRVAHTVAYLGKLRAPIRSVTYTLAQLPCASMALQILWEAAR HL.

In one embodiment, the dTAG has an amino acid sequence derived fromArachidonate 5-lipoxygenase activating protein, UniProtKB-P20292(AL5AP_HUMAN) (incorporated herein by reference), or a variant thereof.In one embodiment, the dTAG is derived from the amino acid sequence:

(SEQ. ID. NO.: 34) MDQETVGNVVLLAIVTLISVVQNGFFAHKVEHESRTQNGRSFQRTGTLAFERVYTANQNCVDAYPTFLAVLWSAGLLCSQVPAAFAGLMYLFVRQKYFVGYLGERTQSTPGYIFGKRIILFLFLMSVAGIFNYYLIFFFGSDFENYIKTI STTISPLLLIP.

In one embodiment, the dTAG has an amino acid sequence derived fromfatty acid binding protein from adipocyte, UniProtKB-P15090(FABP4_HUMAN) (incorporated herein by reference), or a variant thereof.In one embodiment, the dTAG is derived from the amino acid sequence:

(SEQ. ID. NO.: 35) MCDAFVGTWKLVSSENFDDYMKEVGVGFATRKVAGMAKPNMIISVNGDVITIKSESTFKNTEISFILGQEFDEVTADDRKVKSTITLDGGVLVHVQKWDGKSTTIKRKREDDKLVVECVMKGVTSTRVYERA.

In one embodiment, the dTAG has an amino acid sequence derived fromPH-interacting protein, UniProtKB Q8WWQ0 (PHIP HUMAN) (incorporatedherein by reference), or a variant thereof. In one embodiment, the dTAGis derived from the amino acid sequence:

(SEQ. ID. NO.: 36) MSCERKGLSELRSELYFLIARFLEDGPCQQAAQVLIREVAEKELLPRRTDWTGKEHPRTYQNLVKYYRHLAPDHLLQICHRLGPLLEQEIPQSVPGVQTLLGAGRQSLLRTNKSCKHVVWKGSALAALHCGRPPESPVNYGSPPSIADTLFSRKLNGKYRLERLVPTAVYQHMKMHKRILGHLSSVYCVTFDRTGRRIFTGSDDCLVKIWATDDGRLLATLRGHAAEISDMAVNYENTMIAAGSCDKMIRVWCLRTCAPLAVLQGHSASITSLQFSPLCSGSKRYLSSTGADGTICFWLWDAGTLKINPRPAKFTERPRPGVQMICSSFSAGGMFLATGSTDHIIRVYFFGSGQPEKISELEFHTDKVDSIQFSNTSNRFVSGSRDGTARIWQFKRREWKSILLDMATRPAGQNLQGIEDKITKMKVTMVAWDRHDNTVITAVNNMTLKVWNSYTGQLIHVLMGHEDEVFVLEPHPFDPRVLFSAGHDGNVIVWDLARGVKIRSYFNMIEGQGHGAVFDCKCSPDGQHFACTDSHGHLLIFGFGSSSKYDKIADQMFFHSDYRPLIRDANNFVLDEQTQQAPHLMPPPFLVDVDGNPHPSRYQRLVPGRENCREEQLIPQMGVTSSGLNQVLSQQANQEISPLDSMIQRLQQEQDLRRSGEAVISNTSRLSRGSISSTSEVHSPPNVGLRRSGQIEGVRQMHSNAPRSEIATERDLVAWSRRVVVPELSAGVASRQEEWRTAKGEEEIKTYRSEEKRKHLTVPKENKIPTVSKNHAHEHFLDLGESKKQQTNQHNYRTRSALEETPRPSEEIENGSSSSDEGEVVAVSGGTSEEEERAWHSDGSSSDYSSDYSDWTADAGINLQPPKKVPKNKTKKAESSSDEEEESEKQKQKQIKKEKKKVNEEKDGPISPKKKKPKERKQKRLAVGELTENGLTLEEWLPSTWITDTIPRRCPFVPQMGDEVYYFRQGHEAYVEMARKNKIYSINPKKQPWHKMELREQELMKIVGIKYEVGLPTLCCLKLAFLDPDTGKLTGGSFTMKYHDMPDVIDFLVLRQQFDDAKYRRWNIGDRFRSVIDDAWWFGTIESQEPLQLEYPDSLFQCYNVCWDNGDTEKMSPWDMELIPNNAVFPEELGTSVPLTDGECRSLIYKPLDGEWGTNPRDEECERIVAGINQLMTLDIASAFVAPVDLQAYPMYCTVVAYPTDLSTIKQRLENRFYRRVSSLMWEVRYIEHNTRTFNEPGSPIVKSAKFVTDLLLHFIKDQTCYNIIPLYNSMKKKVLSDSEDEEKDADVPGTSTRKRKDHQPRRRLRNRAQSYDIQAWKKQCEELLNLIFQCEDSEPFRQPVDLLEYPDYRDIIDTPMDFATVRETLEAGNYESPMELCKDVRLIFSNSKAYTPSKRSRIYSMSLRLSAFFEEHISSVLSDYKSALRFHKRNTITKRRKKRNRSSSVSSSAASSPERKKRILKPQLKSESSTSAFSTPTRSIPPRHNAAQINGKTESSSVVRTRSNRVVVDPVVTEQPSTSSAAKTFITKANASAIPGKTILENSVKHSKALNTLSSPGQSSFSHGTRNNSAKENMEKEKPVKRKMKSSVLPKASTLSKSSAVIEQGDCKNNALVPGTIQVNGHGGQPSKLVKRGPGRKPKVEVNTNSGEIIHKKRGRKPKKLQYAKPEDLEQNNVHPIRDEVLPSSTCNFLSETNNVKEDLLQKKNRGGRKPKRKMKTQKLDADLLVPASVKVLRRSNRKKIDDPIDEEEEFEELKGSEPHMRTRNQGRRTAFYNEDDSEEEQRQLLFEDTSLTFGTSSRGRVRKLTEKAKANLIGW.

In one embodiment, the dTAG has an amino acid sequence derived fromSUMO-conjugating enzyme UBC9, UniProtKB-P63279 (UBC9_HUMAN)(incorporated herein by reference), or a variant thereof. In oneembodiment, the dTAG is derived from the amino acid sequence:

(SEQ. ID. NO.: 37) MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRA QAKKFAPS.

In one embodiment, the dTAG has an amino acid sequence derived fromProtein S100-A7, UniProtKB-P31151 (S10A7_HUMAN) (incorporated herein byreference), or a variant thereof. In one embodiment, the dTAG is derivedfrom the amino acid sequence:

(SEQ. ID. NO.: 38) MSNTQAERSIIGMIDMFHKYTRRDDKIEKPSLLTMMKENFPNFLSACDKKGTNYLADVFEKKDKNEDKKIDFSEFLSLLGDIATDYHKQSHGAAPCSGG SQ.

In one embodiment, the dTAG has an amino acid sequence derived fromphospholipase A2, membrane associated, UniProtKB-P14555 (PA2GA HUMAN)(incorporated herein by reference), or a variant thereof. In oneembodiment, the dTAG is derived from the amino acid sequence:

(SEQ. ID. NO.: 39) MKTLLLLAVIMIFGLLQAHGNLVNFHRMIKLTTGKEAALSYGFYGCHCGVGGRGSPKDATDRCCVTHDCCYKRLEKRGCGTKFLSYKFSNSGSRITCAKQDSCRSQLCECDKAAATCFARNKTTYNKKYQYYSNKHCRGSTPRC.

In one embodiment, the dTAG has an amino acid sequence derived fromhistone deacetylase 6, UniProtKB-Q9UBN7 (HDAC6_HUMAN) (incorporatedherein by reference), or a variant thereof. In one embodiment, the dTAGis derived from the amino acid sequence:

(SEQ. ID. NO.: 40) MTSTGQDSTTTRQRRSRQNPQSPPQDSSVTSKRNIKKGAVPRSIPNLAEVKKKGKMKKLGQAMEEDLIVGLQGMDLNLEAEALAGTGLVLDEQLNEFHCLWDDSFPEGPERLHAIKEQLIQEGLLDRCVSFQARFAEKEELMLVHSLEYIDLMETTQYMNEGELRVLADTYDSVYLHPNSYSCACLASGSVLRLVDAVLGAEIRNGMAIIRPPGHHAQHSLMDGYCMFNHVAVAARYAQQKHRIRRVLIVDWDVHHGQGTQFTFDQDPSVLYFSIHRYEQGRFWPHLKASNWSTTGFGQGQGYTINVPWNQVGMRDADYIAAFLHVLLPVALEFQPQLVLVAAGFDALQGDPKGEMAATPAGFAQLTHLLMGLAGGKLILSLEGGYNLRALAEGVSASLHTLLGDPCPMILESPGAPCRSAQASVSCALEALEPFWEVLVRSTETVERDNMEEDNVEESEEEGPWEPPVLPILTWPVLQSRTGLVYDQNMMNHCNLWDSHHPEVPQRILRIMCRLEELGLAGRCLTLTPRPATEAELLTCHSAEYVGHLRATEKMKTRELHRESSNFDSIYICPSTFACAQLATGAACRLVEAVLSGEVLNGAAVVRPPGHHAEQDAACGFCFFNSVAVAARHAQTISGHALRILIVDWDVHHGNGTQHMFEDDPSVLYVSLHRYDHGTFFPMGDEGASSQIGRAAGTGFTVNVAWNGPRMGDADYLAAWHRLVLPIAYEFNPELVLVSAGFDAARGDPLGGCQVSPEGYAHLTHLLMGLASGRIILILEGGYNLTSISESMAACTRSLLGDPPPLLTLPRPPLSGALASITETIQVHRRYWRSLRVMKVEDREGPSSSKLVTKKAPQPAKPRLAERMTTREKKVLEAGMGKVTSASFGEESTPGQTNSETAVVALTQDQPSEAATGGATLAQTISEAAIGGAMLGQTTSEEAVGGATPDQTTSEETVGGAILDQTTSEDAVGGATLGQTTSEEAVGGATLAQTTSEAAMEGATLDQTTSEEAPGGTELIQTPLASSTDHQTPPTSPVQGTTPQISPSTLIGSLRTLELGSESQGASESQAPGEENLLGEAAGGQDMADSMLMQGSRGLTDQAIFYAVTPLPWCNILVAVCPIPAAGLDVTQPCGDCGTIQENWVCLSCYQVYCGRYINGHMLQHHGNSGHPLVLSYIDLSAWCYYCQAYVHHQALLDVK NIAHQNKFGEDMPHPH.

In one embodiment, the dTAG has an amino acid sequence derived fromprosaposin, UniProtKB-P07602 (SAP HUMAN) (incorporated herein byreference), or a variant thereof. In one embodiment, the dTAG is derivedfrom the amino acid sequence:

(SEQ. ID. NO.: 41) MYALFLLASLLGAALAGPVLGLKECTRGSAVWCQNVKTASDCGAVKHCLQTVWNKPTVKSLPCDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNMSASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLCESLQKHLAELNHQKQLESNKIPELDMTEVVAPFMANIPLLLYPQDGPRSKPQPKDNGDVCQDCIQMVTDIQTAVRTNSTFVQALVEHVKEECDRLGPGMADICKNYISQYSEIAIQMMMHMQPKEICALVGFCDEVKEMPMQTLVPAKVASKNVIPALELVEPIKKHEVPAKSDVYCEVCEFLVKEVTKLIDNNKTEKEILDAFDKMCSKLPKSLSEECQEVVDTYGSSILSILLEEVSPELVCSMLHLCSGTRLPALTVHVTQPKDGGFCEVCKKLVGYLDRNLEKNSTKQEILAALEKGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDPSFVCLKIGACPSAHKPLLGTEKCIWGPSYWCQNTETAAQCNAVEHCKRHVWN.

In one embodiment, the dTAG has an amino acid sequence derived fromapolipoprotein a, UniProtKB-P08519 (APOA_HUMAN) (incorporated herein byreference), or a variant thereof. In one embodiment, the dTAG is derivedfrom the amino acid sequence:

(SEQ. ID. NO.: 42) MEHKEVVLLLLLFLKSAAPEQSHVVQDCYHGDGQSYRGTYSTTVTGRTCQAWSSMTPHQHNRTTENYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDAVAAPYCYTRDPGVRWEYCNLTQCSDAEGTAVAPPTVTPVPSLEAPSEQAPTEQRPGVQECYHGNGQSYRGTYSTTVTGRTCQAWSSMTPHSHSRTPEYYPNAGLIMNYCRNPDPVAAPYCYTRDPSVRWEYCNLTQCSDAEGTAVAPPTITPIPSLEAPSEQAPTEQRPGVQECYHGNGQSYQGTYFITVTGRTCQAWSSMTPHSHSRTPAYYPNAGLIKNYCRNPDPVAAPWCYTTDPSVRWEYCNLTRCSDAEWTAFVPPNVILAPSLEAFFEQALTEETPGVQDCYYHYGQSYRGTYSTTVTGRTCQAWSSMTPHQHSRTPENYPNAGLTRNYCRNPDAEIRPWCYTMDPSVRWEYCNLTQCLVTESSVLATLTVVPDPSTEASSEEAPTEQSPGVQDCYHGDGQSYRGSFSTTVTGRTCQSWSSMTPHWHQRTTEYYPNGGLTRNYCRNPDAEISPWCYTMDPNVRWEYCNLTQCPVTESSVLATSTAVSEQAPTEQSPTVQDCYHGDGQSYRGSFSTTVTGRTCQSWSSMTPHWHQRTTEYYPNGGLTRNYCRNPDAEIRPWCYTMDPSVRWEYCNLTQCPVMESTLLTTPTVVPVPSTELPSEEAPTENSTGVQDCYRGDGQSYRGTLSTTITGRTCQSWSSMTPHWHRRIPLYYPNAGLTRNYCRNPDAEIRPWCYTMDPSVRWEYCNLTRCPVTESSVLTTPTVAPVPSTEAPSEQAPPEKSPVVQDCYHGDGRSYRGISSTTVTGRTCQSWSSMIPHWHQRTPENYPNAGLTENYCRNPDSGKQPWCYTTDPCVRWEYCNLTQCSETESGVLETPTVVPVPSMEAHSEAAPTEQTPVVRQCYHGNGQSYRGTFSTTVTGRTCQSWSSMTPHRHQRTPENYPNDGLTMNYCRNPDADTGPWCFTMDPSIRWEYCNLTRCSDTEGTVVAPPTVIQVPSLGPPSEQDCMFGNGKGYRGKKATTVTGTPCQEWAAQEPHRHSTFIPGTNKWAGLEKNYCRNPDGDINGPWCYTMNPRKLFDYCDIPLCASSSFDCGKPQVEPKKCPGSIVGGCVAHPHSWPWQVSLRTRFGKEIFCGGTLISPEWVLTAAHCLKKSSRPSSYKVILGAHQEVNLESHVQEIEVSRLFLEPTQADIALLKLSRPAVITDKVMPACLPSPDYMVTARTECYITGWGETQGTFGTGLLKEAQLLVIENEVCNHYKYICAEHLARGTDSCQGDSGGPLVCFEKDKYILQGVTSWGLGCARPNKPGVYARVSRFVTWIEGMMRNN

In one embodiment, the dTAG has an amino acid sequence derived fromlactoglutathione lyase, UniProtKB-Q04760 (LGUL_HUMAN) (incorporatedherein by reference), or a variant thereof. In one embodiment, the dTAGis derived from the amino acid sequence:

(SEQ. ID. NO.: 43) MAEPQPPSGGLTDEAALSCCSDADPSTKDFLLQQTMLRVKDPKKSLDFYTRVLGMTLIQKCDFPIMKFSLYFLAYEDKNDIPKEKDEKIAWALSRKATLELTHNWGTEDDETQSYHNGNSDPRGFGHIGIAVPDVYSACKRFEELGVKFVKKPDDGKMKGLAFIQDPDGYWIEILNPNKMATLM.

In one embodiment, the dTAG has an amino acid sequence derived fromprotein afadin, UniProtKB-P55196 (AFAD_HUMAN) (incorporated herein byreference), or a variant thereof. In one embodiment, the dTAG is derivedfrom the amino acid sequence:

(SEQ. ID. NO.: 44) MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESEILLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLEVIQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK.

In one embodiment, the dTAG has an amino acid sequence derived fromepidermal growth factor receptor (EGFR, UniProtKB P00533(EGFR HUMAN)incorporated herein by reference, or a variant thereof. In oneembodiment, the dTAG is derived from, includes, or is the amino acidsequence:

(SEQ. ID. NO.: 53): (L858R)GEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGRAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQG.

In one embodiment, the dTAG is derived from, includes, or is the aminoacid sequence:

(SEQ. ID. NO.: 54): (T790M)GEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLIMQLMPFGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGRAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQG.In one embodiment, SEQ. ID. NO.: 54 has a Leucine at position 163.

In one embodiment, the dTAG is derived from, includes, or is the aminoacid sequence:

(SEQ. ID. NO.: 55): (C797S)GEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLIMQLMPFGSLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGRAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPS.In one embodiment, SEQ. ID. NO.: 55 has a Leucine at position 163. Inone embodiment, SEQ. ID. NO.: 55 has a Threonine at position 95. In oneembodiment, SEQ. II). NO.: 55 has a Leucine at position 163 and aThreonine at position 95. In one embodiment, the dTAG is derived from,includes, or is the amino acid sequence:

(SEQ. ID. NO.: 56): (C790G)GEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLIMQLMPFGCGLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGRAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQG.In one embodiment, SEQ. ID. NO.: 56 has a Leucine at position 163. Inone embodiment, SEQ. ID. NO.: 56 has a Threonine at position 95. In oneembodiment, SEQ. ID. NO.: 56 has a Leucine at position 163 and aThreonine at position 95.

In one embodiment, the dTAG has an amino acid sequence derived fromepidermal growth factor receptor (BCR-ABL, or a variant thereof. In oneembodiment, the dTAG is derived from, includes, or is the amino acidsequence:

(SEQ. ID. NO.: 57): (T315I)SPNYDKWEMERTDITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIIIEFMTYGNLLDYLRECNRQEVNAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAFIHQAFETMFQES.In one embodiment, SEQ. ID. NO.: 57 has a Threonine at position 87.

In one embodiment, the dTAG has an amino acid sequence derived fromBCR-ABL (BCR-ABL) or a variant thereof. In one embodiment, the dTAG isderived from, includes, or is the amino acid sequence:

(SEQ. ID. NO.: 58): SPNYDKWEMERTDITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECNRQEVNAWLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQAFETMFQES.

In one embodiment, the dTAG has an amino acid sequence derived from ALK(ALK, UniProtKB Q9UM73 (ALK_HUMAN) incorporated herein by reference, ora variant thereof. In one embodiment, the dTAG is derived from,includes, or is the amino acid sequence:

(SEQ ID NO.: 59) (L1196M):ELQSPEYKLSKLRTSTIMTDYNPNYCFAGKTSSISDLKEVPRKNITLIRGLGHGAFGEVYEGQVSGMPNDPSPLQVAVKTLPEVCSEQDELDFLMEALIISKFNHQNIVRCIGVSLQSLPRFIMLELMAGGDLKSFLRETRPRPSQPSSLAMLDLLHVARDIACGCQYLEENHFIHRDIAARNCLLTCPGPGRVAKIGDFGMARDIYRAGYYRKGGCAVILPVKWMPPEAFMEGIFTSKTDTWSFGVLLWEIFSLGYMPYPSKSNQEVLEFVTSGGRMDPPKNCPGPVYRIMTQCVVQHQPEDRPNFAIILERIEYCTQDPDVINTALPIEYGPLVEEEEK.In one embodiment, SEQ. ID. NO.: 59 has a Leucine at position 136.

In one embodiment, the dTAG has an amino acid sequence derived from JAK2(JAK2, UniProtKB O60674 (JAK2_HUMAN) incorporated herein by reference,or a variant thereof. In one embodiment, the dTAG is derived from,includes, or is the amino acid sequence:

(SEQ. ID. NO.: 60) (V617F):VFHKIRNEDLIFNESLGQGTFTKIFKGVRREVGDYGQLHETEVLLKVLDKAHRNYSESFFEAASMNISKLSHKHLVLNYGVCFCGDENILVQEFVKFGSLDTYLKKNKNCINILWKLEVAKQLAWAMHFLEENTLIHGNVCAKNILLIREEDRKTGNPPFIKLSDPGISITVLPKDILQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDSQRKLQFYEDRHQLPAPKAAELANLINNCMDYEPDEIRPSFRAIIRDLNSLFTPD.In one embodiment, SEQ. ID. NO.: 60 has a valine at position 82.

In one embodiment, the dTAG has an amino acid sequence derived from BRAF(BRAF, UniProtKB P15056 (BRAF HUMAN) incorporated herein by reference,or a variant thereof. In one embodiment, the dTAG is derived from,includes, or is the amino acid sequence:

(SEQ. ID. NO 61) (V600E)DWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTAPQLAIVTQWCEGSSLYHHLHASETKFEMKKLIDIARQTARGMDYLHAKSIIHRDLKSNNIFLHEDNTVKIGDFGLATEKSRWSGSHQFEQLSGSILWMAPEVIRMQDSNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIEMVGRGSLSPDLSKVRSNCPKRMKRLMAECLKKKRDERPSFPRILAEIEELARE.In one embodiment, SEQ. ID. NO.: 61 has a Valine at position 152. In oneembodiment, SEQ. ID. NO. 61 has a Tyrosine at position 153. In oneembodiment, SEQ. ID. NO.: 61 has a Valine at position 152. In oneembodiment, SEQ. ID. NO. 61 has a Lysine at position 153. In oneembodiment, SEQ. ID. NO.: 61 has a Valine at position 152 and a Lysineat position 153.

In one embodiment, the dTAG has an amino acid sequence derived from aLRRK2 protein (UniProtKB Q5S007 (LRRK2_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom LRRK2 amino acid 1328 to 1511. In one embodiment, the dTAG isderived from LRRK2 amino acid 1328 to 1511, wherein amino acid 1441 isCysteine

In one embodiment, the dTAG has an amino acid sequence derived from aPDGFRa protein (UniProtKB-P09619 (PDGFRHUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 600 to 692 of P09619. In one embodiment, the dTAG isderived from amino acid 600 to 692 of P09619, wherein amino acid 674 isIsoleucine.

In one embodiment, the dTAG has an amino acid sequence derived from aRET protein (UniProtKB-P07949 (RET_HUMAN) incorporated herein byreference), or variant thereof. In one embodiment, the dTAG is derivedfrom amino acid 724 to 1016 of P07949. In one embodiment, the dTAG isderived from amino acid 724 to 1016 of P07949, wherein amino acid 691 isSerine. In one embodiment, the dTAG is derived from amino acid 724 to1016 of P07949, wherein amino acid 749 is Threonine. In one embodiment,the dTAG is derived from amino acid 724 to 1016 of P07949, wherein aminoacid 762 is Glutamine. In one embodiment, the dTAG is derived from aminoacid 724 to 1016 of P07949, wherein amino acid 791 is Phenylalanine. Inone embodiment, the dTAG is derived from amino acid 724 to 1016 ofP07949, wherein amino acid 804 is Methionine. In one embodiment, thedTAG is derived from amino acid 724 to 1016 of P07949, wherein aminoacid 918 is Threonine.

In one embodiment, the dTAG has an amino acid sequence derived from aJAK3 protein (UniProtKB-P52333 (JAK3_HUMAN) incorporated herein byreference), or variant thereof.

In one embodiment, the dTAG has an amino acid sequence derived from aABL protein (UniProtKB-P00519 (ABL_HUMAN) incorporated herein byreference), or variant thereof.

In one embodiment, the dTAG has an amino acid sequence derived from aMEK1 protein (UniProtKB-Q02750 (MP2K1_HUMAN) incorporated herein byreference), or variant thereof.

In one embodiment, the dTAG has an amino acid sequence derived from aKIT protein (UniProtKB-P10721 (KIT_HUMAN) incorporated herein byreference), or variant thereof.

In one embodiment, the dTAG has an amino acid sequence derived from aKIT protein (UniProtKB-P10721 (KIT_HUMAN) incorporated herein byreference), or variant thereof.

In one embodiment, the dTAG has an amino acid sequence derived from aHIV reverse transcriptase protein (UniProtKB-P04585 (POL_HV1H2)incorporated herein by reference), or variant thereof.

In one embodiment, the dTAG has an amino acid sequence derived from aHIV integrase protein (UniProtKB-Q76353 (Q76353_9HIV1)) incorporatedherein by reference), or variant thereof.

B. Proteins of Interest

As contemplated herein, the dTAG strategy can be utilized to produce astably expressed, endogenous protein-dTAG hybrid in vivo, or as the casemay be ex vivo or in vitro, by genomic insertion of the dTAG nucleicacid sequence either 5′- or 3′ in-frame with the nucleic acid sequenceencoding the protein of interest. Following the insertion of thein-frame dTAG nucleic acid sequence, the cell expresses the endogenousprotein-dTAG hybrid, allowing for the modulation of the activity of theendogenous protein-dTAG hybrid through the administration of aheterobifunctional compound that is capable of binding the dTAG and thusdegrading the endogenous protein-dTAG hybrid. In one embodiment, theactivity of the endogenous protein-dTAG hybrid is reduced.

In certain embodiments, a nucleic acid encoding a dTAG can begenomically inserted in-frame with a gene encoding a protein that isinvolved in a disorder. Non-limiting examples of particular genesinvolved in disorders that may be targeted for dTAG insertion include byway of non-limiting example, alpha-1 antitrypsin (A1AT), apolipoproteinB (APOB), angiopoietin-like protein 3 (ANGPTL3), proprotein convertasesubtilisin/kexin type 9 (PCSK9), apolipoprotein C3 (APOC3), catenin(CTNNB1), low density lipoprotein receptor (LDLR), C-reactive protein(CRP), apolipoprotein a (Apo(a)), Factor VII, Factor XI, antithrombinIII (SERPINC1), phosphatidylinositol glycan class A (PIG-A), C5, alpha-1antitrypsin (SERPINA1), hepcidin regulation (TMPRSS6),(delta-aminolevulinate synthase 1 (ALAS-1), acylCaA:diacylglycerolacyltransferase (DGAT), miR-122, miR-21, miR-155, miR-34a, prekallikrein(KLKB1), connective tissue growth factor (CCN2), intercellular adhesionmolecule 1 (ICAM-1), glucagon receptor (GCGR), glucorticoid receptor(GCCR), protein tyrosine phosphatase (PTP-1B), c-Raf kinase (RAF1),fibroblast growth factor receptor 4 (FGFR4), vascular adhesionmolecule-1 (VCAM-1), very late antigen-4 (VLA-4), transthyretin (TTR),survival motor neuron 2 (SMN2), growth hormone receptor (GHR), dystophiamyotonic protein kinase (DMPK), cellular nucleic acid-binding protein(CNBP or ZNF9), clusterin (CLU), eukaryotic translation initiationfactor 4E (eIF-4e), MDM2, MDM4, heat shock protein 27 (HSP 27), signaltransduction and activator of transcription 3 protein (STAT3), vascularendothelial growth factor (VEGF), kinesin spindle protein (KIF11),hepatitis B genome, the androgen receptor (AR), Atonal homolog 1(ATOH1), vascular endothelial growth factor receptor 1 (FLT1),retinoschism 1 (RS1), retinal pigment epithelium-specific 65 kDa protein(RPE65), Rab escort protein 1 (CHM), and the sodium channel, voltagegated, type X, alpha subunit (PN3 or SCN10A). Additional proteins ofinterest that may be targeted by dTAG insertion include proteinsassociated with gain of function mutations, for example, cancer causingproteins.

In particular embodiments, the protein of interest for targeting isapoB-100, ANGPTL3, PCSK9, APOC3, CRP, ApoA, Factor XI, Factor VII,antithrombin III, phosphatidylinositol glycan class A (PIG-A), the C5component of complement, Alpha-1-antitrypsin (A1AT), TMPRSS6, ALAS-1,DGAT-2, KLB1, CCN2, ICAM, glucagon receptor, glucocorticoid receptor,PTP-1B, FGFR4, VCAM-1, VLA-4, GCCR, TTR, SMN1, GHR, DMPK, or NAV1.8.

In one embodiment, the dTAG is genomically integrated in-frame, either5′ or 3′, into the gene encoding for an endogenous protein associatedwith a proteopathy. In one embodiment the dTAG is genomically integratedin-frame, either 5′ or 3′, into the gene encoding for an endogenousprotein associated with a disorder selected from is genomically insertedin-frame, either 5′ or 3′, into the gene encoding for an endogenousprotein associated with Alzheimer's disease (Amyloid β peptide (Aβ); Tauprotein), Cerebral β-amyloid angiopathy (Amyloid β peptide (A13)),Retinal ganglion cell degeneration in glaucoma (Amyloid β peptide (Aβ)),Prion diseases (Prion protein), Parkinson's disease and othersynucleinopathies (α-Synuclein), Tauopathies (Microtubule-associatedprotein tau (Tau protein)), Frontotemporal lobar degeneration (FTLD)(Ubi+, Tau-) (TDP-43), FTLD-FUS (Fused in sarcoma (FUS) protein),Amyotrophic lateral sclerosis (ALS) (Superoxide dismutase, TDP-43, FUS),Huntington's disease and other triplet repeat disorders (Proteins withtandem glutamine expansions), Familial British dementia (ABri), FamilialDanish dementia (Adan), Hereditary cerebral hemorrhage with amyloidosis(Icelandic) (HCHWA-I) (Cystatin C), CADASIL (Notch3), Alexander disease(Glial fibrillary acidic protein (GFAP)), Seipinopathies (Seipin),Familial amyloidotic neuropathy, Senile systemic amyloidosis(Transthyretin), Serpinopathies (Serpins), AL (light chain) amyloidosis(primary systemic amyloidosis) (Monoclonal immunoglobulin light chains),AH (heavy chain) amyloidosis (Immunoglobulin heavy chains), AA(secondary) amyloidosis (Amyloid A protein), Type II diabetes (Isletamyloid polypeptide (IAPP; amylin)), Aortic medial amyloidosis (Medin(lactadherin)), ApoAI amyloidosis (Apolipoprotein AI), ApoAIIamyloidosis (Apolipoprotein AII), ApoAIV amyloidosis (ApolipoproteinAIV), Familial amyloidosis of the Finnish type (FAF) (Gelsolin),Lysozyme amyloidosis (Lysozyme), Fibrinogen amyloidosis (Fibrinogen),Dialysis amyloidosis (Beta-2 microglobulin), Inclusion bodymyositis/myopathy (Amyloid β peptide (Aβ)), Cataracts (Crystallins),Retinitis pigmentosa with rhodopsin mutations (rhodopsin), Medullarythyroid carcinoma (Calcitonin), Cardiac atrial amyloidosis (Atrialnatriuretic factor), Pituitary prolactinoma (Prolactin), Hereditarylattice corneal dystrophy (Keratoepithelin), Cutaneous lichenamyloidosis (Keratins), Mallory bodies (Keratin intermediate filamentproteins), Corneal lactoferrin amyloidosis (Lactoferrin), Pulmonaryalveolar proteinosis (Surfactant protein C (SP-C)), Odontogenic(Pindborg) tumor amyloid (Odontogenic ameloblast-associated protein),Seminal vesicle amyloid (Semenogelin I), Cystic Fibrosis (cysticfibrosis transmembrane conductance regulator (CFTR) protein), Sicklecell disease (Hemoglobin), and Critical illness myopathy (CIM)(Hyperproteolytic state of myosin ubiquitination).

As contemplated herein, by genomically inserting a nucleic acid encodinga dTAG in frame with particular proteins of interest, modulation of theprotein of interest can be achieved by administering aheterobifunctional compound specific for the dTAG, which binds to theprotein-dTAG hybrid, leading to its degradation. Because of the abilityto modulate a particular protein of interest in this manner, such astrategy can be used to treat disorders wherein expression of a proteinabove certain threshold levels within the cell leads to a diseasedstate. Other applications of this technology include, but are notlimited to 1.) targeted degradation of proteins where pathology is afunction of gain of function mutation(s), 2) targeted degradation ofproteins where pathology is a function of amplification or increasedexpression, 3) targeted degradation of proteins that are manifestationsof monogenetic disease, 4) targeted degradation of proteins wheregenetic predisposition manifests over longer periods and often afteralternative biological compensatory mechanisms are no longer adequate,for example, but not limited to, hypercholesterolemia andproteinopathies.

By controlled degradation of the endogenous protein-dTAG hybrid, afavorable change in protein expression or activity kinetics may resultin prevention and/or treatment of a disorder in a subject in needthereof.

Exemplary diseases and disorders capable of being treated by thecurrently contemplated methods are described, for example, in U.S.Application No. 20150329875 titled “Methods and Compositions forPrevention of Treatment of a Disease,” incorporated herein by reference.

In certain embodiments, the target proteins are involved in lipidmetabolism. For example, hypercholesterolemia is a conditioncharacterized by very high levels of cholesterol in the blood which isknown to increase the risk of coronary artery disease. Familialhypercholesterolemia, hyperlipidemia, and familial chylomicronemia aregenetic conditions passed through families where an aberrant gene causesthe observed symptomology. Mutations in genes encoding the LDL receptor(LDLR), Apoliprotein B (APOB), angiopoietin-like protein 3 (ANGPTL3) andproprotein convertase subtilisin/kexin type 9 (PCSK9) are involved inthese diseases. The LDLR serves to remove LDL from the plasma forinternalization into the cell. The LDLR is a transmembrane protein thatlocalizes to clathrin-coated pits where it forms a complex with ApoB-100(the longer gene product of APOB) and apoE enriched lipoproteins.Following endocytosis of this complex, it moves to the endosome wherethe lipoproteins are released from the complex for eventual degradationby the lysosome. The LDLR can then be recycled back to the cell surface.

Patients with defective apoB-100, termed ‘Familial defectiveapolipoprotein B’ (FDB), frequently carry a R3500Q mutation in APOBwhich makes LDL with reduced ability to bind to the LDLR, reducingplasma clearance, thus raising plasma levels of fatty acids (Innerarityet al, (1987) PNAS USA 84:6919). FDB is generally recognized as anautosomal dominant condition, and occurs in approximately 1:700 peopleof European descent (Ginsburg and Willard (2012) Genomic andPersonalized Medicine, volumes 1 and 2. Academic Press, London. p. 507).Thus, in FDB patients that are heterozygous for the mutation atapoB-100, specific degradation of the defective apoB-100 allele byinserting a dTAG in-frame in the allele in liver cells and administeringa heterobifunctional compound, resulting in the gene product of anapo-100 defective protein-dTAG hybrid, can cause correction of thedisease.

Similarly, angiopoietin-like protein 3 (ANGPTL3) overexpressionmutations that cause elevated levels of ANGPTL3 can cause hyperlipidemiain subjects. ANGPTL3 also acts as dual inhibitor of lipoprotein lipase(LPL) and endothelial lipase (EL), and increases plasma triglyceride andHDL cholesterol in rodents. ANGPTL3 is expressed primarily in the liverand secreted, and normally acts to increase plasma levels oftriglycerides, LDL cholesterol and HDL cholesterol where it actsdirectly on the liver to regulate hepatocellular lipoprotein secretionand clearance (Musunuru et at (2010) N Engl J Med 363:23 p. 2220). Thus,the method of the invention can be used to treat hyperlipidemia relatedto ANGPTL3 overexpression through the targeted degradation of theprotein using the dTAG insertion strategy described herein.

PCSK9 is another gene encoding a protein that plays a major regulatoryrole in cholesterol homeostasis. PCSK9 binds to the epidermal growthfactor-like repeat A (EGF-A) domain of LDLR, and induces LDLRdegradation. Autosomal dominant, toxic gain of function mutations inPCSK9 (e.g. S127R, P216L, D374Y and N157K) have been described and areassociated with hyperlipidemia and Familial hypercholesterolemia (FH) asa result of an increased rate of LDLR degradation leading to acorresponding increase in plasma LDL cholesterol (Abifadel et at (2003)Nat Gen 34(2):154). In addition, loss of function PCSK9 mutations havebeen identified (e.g. Y142X, C679X and R46L) that cause an increase inhepatic LDLR levels, with an associated substantial decrease in theamount of plasma LDL cholesterol, leading to an 88% reduction in theincidence of coronary heart disease (Cohen et at (2003) New Eng J Med354(12): 1264). Thus the methods and compositions of the invention canbe used to treat or prevent hyperlipidemia and/or FH through thetargeted degradation of the PCSK9 protein using the dTAG insertionstrategy described herein.

Familial chylomicronemia syndrome, or FCS, is characterized by extremelyhigh levels of plasma triglycerides and lead to a number of healthproblems such as abdominal pain, enlargement of the liver and spleen andrecurrent acute pancreatitis. In addition, there are subjects with hightriglyceride levels that do not have FCS, but, due to the elevatedtriglycerides, have similar health issues. Apolipoprotein C3, orapo-CIII, encoded by the APOC3 gene, is a component of very lowlipoprotein (VLDL), LDL, HDL and chylomicrons, and normally inhibitslipolysis by inhibiting lipoprotein lipase and hepatic lipase. Apo-CIIIinhibits hepatic uptake of triglyceride-rich particles and can beelevated in patients with hyperlipidemia (Bobik, (2008) Circulation118:702) and is an independent cardiovascular disease risk factor.Knocking out the APOC3 agene in mice results in animals with reducedplasma triglyceride levels as compared to normal (Maeda et al (1994) JBiol Chem 269(38):23610). Thus, the methods and compositions of theinvention can be used to prevent or treat a subject with lipidmetabolism disorders (e.g., familial hypercholesterolemia,hyperlipidemia, and familial chylomicronemia) by targeted degradation ofthe APOC3 protein through use of the dTAG insertion strategy describedherein.

In other embodiments, the target protein(s) are involved in vasculardiseases such as cardiovascular disease and coronary artery disease.Similar to the lipid metabolism disorders discussed above, coronaryartery diseases can also be caused by specific genes. For example,C-reactive protein (CRP) is a protein produced in the liver that hasbeen associated with inflammatory disease. It is an acute phase proteinthat binds to phosphocholine expressed on the surface of dead or dyingcells where its job is to activate the complement system to help clearthe cell. In chronic inflammatory disease, increased levels of CRP mayexacerbate disease symptoms by contributing and amplifying an overallchronic inflammatory state. In addition, it has been shown in rat modelsthat CRP increases myocardial and cerebral infarct size, which, whentranslated into human patients, maybe predicative of a more negativeprognosis following heart attack. When inhibitors of CRP are introducedinto these rat models, infarct size and cardiac dysfunction aredecreased (Pepys et at (2005) Nature 440(27):1217). Inhibition of CRPthus may be beneficial both in inflammatory diseases and in coronaryartery disease. The methods and compositions of the invention may beused to cause modulation of CRP expression by targeted degradation ofthe CRP protein through use of the dTAG insertion strategy describedherein.

Plasma lipoprotein (Lp(a)) is a low density lipoprotein particlecomprising Apolipoprotein(a) (apo(a)), and is also an independent riskfactor for cardiovascular disease including atherosclerosis. Apo(a)contacts the surface of LDL through apoB-100, linked by a disulfidebond, and it has been reported that genetic polymorphisms associatedwith elevated Apo(a) levels are associated with an excessive rate ofmyocardial infarction (Chasman et at (2009) Atherosclerosis 203(2):371).Lp(a) concentration in the plasma varies widely in concentration betweenindividuals, where these concentration differences appear to begenetically determined. The apo(a) gene comprises a number ofplasminogen kringle 4-like repeats, and the number of these kringlerepeats is inversely related to plasma concentration of Lp(a). ADNA-vaccine approach, designed to mount an immune response to apo(a) andcause antibody-mediated clearance of Lp(a), demonstrated a reduction inthe proatherosclerotic activity of Lp(a) in mice (Kyutoku et at (2013)Sci Rep 3 doi: 10.1038/srep1600). Thus the methods and compositions ofthe invention can be used to reduce the expression of the ApoA protein,resulting in a decrease in plasma concentration of Lp(a), by targeteddegradation of the ApoA protein through use of the dTAG insertionstrategy described herein.

Clotting disorders, often referred to as thrombophilia, can haveramifications in vascular diseases. The complex network of biochemicalevents regulating mammalian coagulation comprises 5 proteases (factorsII, VII, IX, and X and protein C) that interface with 5 cofactors(tissue factor, factor V, factor VIII, thrombomodulin, and surfacemembrane proteins) to generate fibrin, which is the main component of aclot. A delicate balance exists between powerful endogenous procoagulantand thromboresistant forces to ensure the fluidity of blood and maintainthe readiness of these factors to induce a blood clot if an injuryoccurs. High plasma activity of both Factor XI and Factor VII areassociated with hypercoagulation and thrombotic disease (coronaryinfarcts, stroke, deep vein thrombosis, pulmonary embolism) and withpoor patient prognosis. It has been demonstrated that people that withsevere Factor XI deficiency are protected from ischemic brain injury andstroke (Saloman et at (2008) Blood 111:4113). At the same time, it hasbeen shown that high levels of FXI are associated with higher rates ofstroke incidents in patients (Yang et at (2006) Am J Clin Path 126:411). Similarly, high Factor VII levels are also associated withcoronary artery disease although this is complicated by otherconsiderations such as how the Factor VII is measured, and which form ofthe protein is analyzed (Chan et at (2008) Circulation 118:2286). Thus,the methods and compositions of the invention can be used to prevent ortreat subjects with hyperthrombotic disease through selectivedegradation of clotting factors associated with the disease (forexample, Factor VII and Factor XI) by targeted degradation of Factor XIand/or Factor VII through use of the dTAG insertion strategy describedherein.

As described above, the balance of the clotting cascade is crucial.Thus, in addition to the importance of the clotting factors, theinhibitors of these factors are also critical. Patients with hemophiliasare deficient in one or more components of the clotting cascade, andhave a reduced clotting capacity as a consequence. In one of the laststeps of this cascade, thrombin acts on fibrinogen to create fibrinwhich is the main component of the clot. The cascade leads up to theproduction of active thrombin to allow this to occur. To keep the systembalanced, antithrombin (also known as antithrombin III, encoded by theSERPINC1 gene) acts upon thrombin to inhibit its action. In manyhemophilias, the factor deficiency is not absolute and there is somedegree of clotting that occurs. Thus an approach based on degradation ofantithrombin could allow the clotting cascade to produce sufficientclotting when the upstream factors are limited, potentially regardlessof which factor is deficient. This has been demonstrated using bloodderived from hemophilia A patients (see Di Micco et at (2000) Eur JPharmacol. March 10; 391(1-2): 1-9.). The methods and compositions ofthe invention can be used to treat patients with hemophilias such asHemophilia A and Hemophilia B by targeted degradation of theantithrombin III protein through use of the dTAG insertion strategydescribed herein.

The target protein(s) may also be involved in blood disorders(hematological conditions). The complement system is a pivotal player inmultiple hematological conditions. Paroxysmal nocturnal hemoglobinuria(PNH) is a hemolytic disease caused by a defect in the PIG-A gene (seeBrodsky (2008) Blood Rev 22(2):65). The PIG-A gene productphosphatidylinositol glycan class A is required for the first step inthe synthesis of GPI-anchored proteins. PIG-A is found on the Xchromosome and mutations in PIG-A result in red blood cells that aresensitive to hemolysis by complement. Notably, these mutant cells lackthe GPI-anchored proteins CD55 and CD59. CD59 interacts directly withthe complement related membrane attack complex (or MAC) to prevent lyticpore formation by blocking the aggregation of C9, a vital step in theassembly of the pore. CD55 functions to accelerate the destruction ofthe C3 convertase, so in the absence of CD55, there is more of the C3convertase enzyme, leading to more MAC formation. Thus, the lack of bothof these proteins leads to increases lysis of the mutant red bloodcells. For patients with PNH, complications due to increased thrombosisare the greatest concern (Brodsky (2008) Blood Rev 22(2):65). 40% of PNHpatients have ongoing thrombosis which can lead to stroke and acutecardiovascular disease. Thus, the methods and compositions of theinventions can be used to treat and/or prevent PHN in a subject bytargeted degradation of the phosphatidylinositol glycan class A (PIG A)through use of the dTAG insertion strategy described herein.

Inhibition of the C5 component of complement has been approved as atreatment for both PNH and atypical hemolytic-uremic syndrome (aHUS),validating C5 as an important therapeutic target. The hemolysis of redblood cells associated with aHUS occurs when the cells are targeted fordestruction by the alternative pathway due to a dysregulation of thecomplement system (part of innate immunity). Normally the destructiveC3bBb complex is formed on the surface of an invading cell (e.g. abacterium) to hasten its destruction as part of the alternative pathwayin the complement system. The C3bBb complex can bind another C3b to forma C3bBbC3b complex which then acts as a C5 convertase. C5 convertasecleaves C5 to C5a and C5b, and C5b recruits C6, C7, C8 and C9 to formthe MAC. A set of complement regulatory proteins (e.g. CD35, CD46, CD55and CD59) are located on the body's own cells to inhibit the activity ofthese proteins and thus protect them. However, when there is animbalance of these regulatory proteins, the C3bBb complex can forminappropriately (de Jorge et at (2011) J Am Soc Nephrol 22:137). Thissyndrome, in addition to the premature destruction of red blood cellscan also lead to kidney disease as a result of the damaging and cloggingof the glomerular filtering apparatus. C5 negative mice were shown to beprotected when crossed with mice with complement regulator proteinmutations, data that has been used to validate the idea of C5 as atarget in aHUS (de Jorge, ibid) and other diseases related to complementdysregulation. The C5b-specific monoclonal antibody eculizamab has beensuccessfully used to treat aHUS (Gruppo and Rother, (2009) N Engl J Med360; 5 p 544) and other complement-mediated diseases (e.g. ParoxysmalNocturnal Haemoglobinuria (PNH) (Hillmen et al, (2013) Br. J Haem162:62)). Thus, the methods and compositions of the invention can beused to modulate the expression of C5 and so prevent or treat diseasesassociated with complement dysregulation by targeted degradation of C5through use of the dTAG insertion strategy described herein.

Alpha-1-antitrypsin (A1AT) deficiency occurs in about 1 in 1500-3000people of European ancestry but is rare in individuals of Asian descent.The alpha-1-antitrypsin protein is a protease inhibitor that is encodedby the SERPINA1 gene and serves to protect cells from the activity ofproteases released by inflammatory cells, including neutrophil elastase,trypsin and proteinase-3 (PR-3). Deficiency is an autosomal co-dominantor a recessive disorder caused by mutant SERPINA1 genes in heterozygousindividuals where reduced expression from the mutant allele or theexpression of a mutant A1AT protein with poor inhibitory activity leadsto chronic lack of inhibition of neutrophil elastase resulting in tissuedamage. The most common SERPINA1 mutation comprises a Glu342Lyssubstitution (also referred to as the Z allele) that causes the proteinto form ordered polymers in the endoplasmic reticulum of patienthepatocytes. These inclusions ultimately cause liver cirrhosis which canonly be treated by liver transplantation (Yusa et at (2011) Nature 478p. 391). The polymerization within the hepatocytes results in a severedecrease in plasma A1AT levels, leading to increased risk of thisinflammatory disease. In addition, A1AT deficiency is linked topulmonary diseases including chronic obstructive pulmonary disease(COPD), emphysema and chronic bronchitis (Tuder et at (2010) Proc AmThorac Soc 7(6): p. 381) and potentially may have a far broader reachinto the inhibition of the progression of other diseases including type1 and type 2 diabetes, acute myocardial infarction, rheumatoidarthritis, inflammatory bowel disease, cystic fibrosis, transplantrejection, graft versus host disease and multiple sclerosis (Lewis(2012) Mol Med 18(1) p. 957). Population studies have suggested aminimum ATA1 plasma threshold of approximately 0.5 mg/mL (normal plasmalevels are approximately 0.9-1.75 mg/ML in a non-inflammatory state) toavoid these diseases, and current therapies mostly act to reducesymptoms through the use of bronchodilators and the like, although theuse of weekly infusions of A1AT (Zemaira®) is also an option foremphysema patients with a demonstrated severe lack of plasma A1AT.Severe lung disease associated with A1AT also is ultimately treated bytransplant. Clinical trials for the treatment of A1AT deficiency involvea variety of approaches including the delivery of concentrated A1ATprotein, use of an AAV construct comprising an A1AT gene by IMinjection, and the use of A1AT in HIV, to list just a few. Thus, thecompositions and methods of the invention can be used to treat orprevent diseases related to A1AT deficiency by targeted degradation ofalpha-1-antitrypsin protein through use of the dTAG insertion strategydescribed herein, thereby eliminating the hepatic aggregates that canlead to cirrhosis.

Another liver target of interest includes any protein(s) that is(are)involved in the regulation of iron content in the body. Iron isessential for the hemoglobin production, but in excess can result in theproduction of reactive oxygen species. In patients that are dependent onblood transfusions (e.g. certain hemophilias, hemoglobinopathies),secondary iron overload is common. The iron-regulatory hormone hepcidin,and its receptor and iron channel ferroportin control the dietaryabsorption, storage, and tissue distribution of iron by promoting itscellular uptake. The regulation of hepcidin is done at a transcriptionallevel, and is sensitive to iron concentrations in the plasma whereincreased hepcidin expression leads to lower plasma iron concentrations.Through a series of receptor-ligand interactions, involving a receptorknown as hemojuvelin, the hepcidin gene is upregulated by a SMADtranscription factor. Iron-related hepcidin down regulation in turn isregulated by a protease known as TMPRSS6, which cleaves hemojuvelin andprevents the upregulation of hepcidin (Ganz (2011) Blood 117:4425). Downregulation of TMPSS6 expression by use of an inhibitory RNA targetingthe TMRSS6 mRNA has been shown to cause a decrease in iron overload inmouse models (Schmidt et al (2013) Blood 121:1200). Thus, the methodsand compositions of the invention can be used to target TMPRSS6 fordegradation through use of the dTAG insertion strategy described herein.

Other conditions related to iron utilization pathways in the body areporphyrias. These disorders result from a number of deficiencies in theenzymes involved in heme synthesis. Acute intermittent porphyia (AIP) isan autosomal dominant disorder and is the second most common porphyria,with an incidence of approximately 5-10 in 100,000 people. AIP is causedby a deficiency in hydroxymethylbilane synthase (HMB synthase (HMBS),also called porphobilinogen-deaminase), where the mutations in the HMBSgene are very heterogeneous, comprising missense and point mutations(Solis et al (1999) Mol Med 5:664). The potentially life-threatening AIPattacks can have gastrointestinal, neuropsychiatric, cardiovascular andnervous system manifestations. Attacks have several triggers, can lastfor several days, and often require hospitalization and can beprecipitated by several seemingly unrelated factors including certaindrugs, infection, caloric restriction, smoking, alcohol and hormonalfluctuations relating to the menstrual cycle (Yasuda et al (2010) MolTher 18(1):17). HMB synthase is part of the heme synthesis pathway,where glycine and succinyl-CoA are joined by delta-aminolaevulinatesynthase 1 (ALAS-1) to make aminolevulinic acid, which is then actedupon by aminolevulinic acid dehydratase (ALAD) to make phosphobilinogen.Phosphobilinogen is the converted to hydroxymethylbilane by HMBsynthase. The pathway continues on from there, ultimately producing theheme (Ajioka et at (2006) Biochim Biophys Acta 1762:723). Regardless ofthe trigger, all attacks result in an elevation of the enzymedelta-aminolevulinate synthase 1 (ALAS-1). This enzyme is the firstenzyme in the hepatic heme synthesis pathway and when induced, thedeficiency in HMB synthase becomes rate-limiting and the aminolevulinicacid and phosphobilinogen precursors accumulate (Yasuda, ibid). Livertransplant in AIP patients can stop the attacks, indicating thattargeting the liver may be therapeutically beneficial. Additionally, inmouse models of AIP, where the mice have only 30% of normal HMB synthaselevels, insertion of the transgene HMBS, encoding HMB synthase, resultedin a decrease in aminolevulinic acid and phosphobilinogen accumulationwhen the mice were given phenobarbital (Yasuda, ibid). Double strandedRNAs designed for the inhibition of ALAS-1 have also been shown toreduce ALAS-1 expression in vivo in a mouse AIP model and to reducephosphobilinogen accumulation in response to phenobarbital treatment(see U.S. Patent Publication 20130281511). Thus the methods andcompositions of the invention may be used to prevent and treat AIP bytargeted degradation of ALAS-1 using the dTAG insertion strategydescribed herein.

Non-alcoholic fatty liver disease (NAFLD) is the most common form ofliver disease worldwide, with a prevalence of 15%-30% in Westernpopulations and is caused by triglyceride accumulation within the liver.However, the prevalence increases to 58% in overweight populations and98% in obese populations. Nonalcoholic steatohepatitis (NASH) is a moreadvanced form of NAFLD where liver injury has occurred, and can lead toliver failure, portal hypertension, hepatocarcinoma and cirrhosis(Schwenger and Allard (2014) World J Gastronen 20(7): 1712). Evidenceappears to suggest that the hepatic triglyceride accumulation observedin NALFD is strongly associated with hepatic insulin resistance, oftenas a part of type 2 diabetes and metabolic syndrome (Choi et at (2017, JBiol Chem 282 (31): 22678). Acyl-CaA:diacylglycerol acyltransferase(DGAT) catalyzes the final step in triglyceride synthesis byfacilitating the linkage of sn-1,2 diacylglygerol (DAG) with a longchain acyl CoA. There are two primary isoforms of DGAT, DGAT-1 andDGAT-2. DGAT-1 is primarily expressed in the small intestine whileDGAT-2 exhibits primarily hepatic expression where its expression isinsulin responsive. Knock down of expression of DGAT-1 or DGAT-2 usingantisense oligonucleotides in rats with diet-induced NALFD significantlyimproved hepatic steatosis in the DGAT-2 knockdowns but not the DGAT-1knockdowns (Choi, ibid). Thus, the materials and methods of theinvention can be used to alter expression of DGAT-2 for the treatment ofNASH and NALFD, and to reduce hepatic insulin resistance by targeteddegradation of DGAT-2 using the dTAG insertion strategy describedherein.

Further vascular targets include those involved in hereditary angioedema(HAE). HAE is an autosomal dominant disease that affects 1 in 50,000people and is a result of decreased levels of the C1 inhibitor. Patientsexperience recurrent episodes of swelling in any part of the body whereswelling localized to the oropharynx, larynx or abdomen carry thehighest risk of morbidity and death (see Tse and Zuraw, (2013) Clev ClinJ of Med 80(5):297). The disease occurs from extravasation of plasmainto tissues as a result of the over production of bradykinin. Themechanism seems to involve the cleavage of prekallikrein (also known asPKK) by activate factor XII, releasing active plasma kallikrein (whichactivates more factor XII). Plasma kallikrein then cleaves kininogen,releasing bradykinin. The bradykinin then binds to the B2 bradykininreceptor on endothelial cells, increasing the permeability of theendothelium. Normally, the C1 inhibitor (encoded by SERPING1) controlsbradykinin production by inhibiting plasma kallikrein and the activationof factor XII. HAE occurs in three types, Type I and II that aredistinguished by the amount and type of C1 inhibitor present, and TypeIII which is tied to a Thr309Lys mutation in factor XII (Prieto et at(2009) Allergy 64(2):284). Type I HAE has low levels of C1 inhibitorthat appear to be a result of poor expression and destruction of thesmall amount of C1 inhibitor protein that is made. Type 1 accounts forapproximately 85% of HAE patients. Type II patients have normal levelsof C1 inhibitor, but the C1 inhibitor protein is ineffectual due tomutations (Tse and Zuraw, ibid). More than 250 mutations in SERPING1have been characterized that lead to Type I HAE including small andlarge insertions and deletions as well as duplications (Rijavec et at(2013) PLoS One 8(2): e56712). Due to this high variability in thegenetic basis of HAE, the methods and compositions of the invention canbe used to prevent or treat HAE by targeting downstream players in themanifestation of HAE. For example, targeting prekallikrein (KLKB1,expressed in hepatocytes) to effect a decrease in prekallikrein(abbreviated PKK) expression can result in a decrease in bradykininproduction without regard to the type of mutation upstream that iscausing the HAE, and thus result in a decrease in plasma extravasation.Thus, the methods and compositions of the invention may be used to causea decrease in the expression of KLKB1 to prevent or treat HAE bytargeted degradation of KLKB1 using the dTAG insertion strategydescribed herein.

Target(s) may also be involved in a fibrotic disease. Fibrotic diseasein various organs is the leading cause of organ dysfunction and canoccur either as a reaction to another underlying disease or as theresult of a predisposition towards fibrosis in an afflicted individual.The hallmark of fibrosis is the inappropriate deposition ofextracellular matrix compounds such as collagens and relatedglycoproteins. TGF-β plays a major role in the fibrotic process,inducing fibroblasts to synthesize extracellular matrix (ECM) proteins,and it also inhibits the expression of proteins with ECM break downactivity (Leask (2011) J Cell Commun Signal 5:125). There is a class ofECM regulatory proteins known as the CNN proteins (so-called because thefirst three members are described, namely CYR61 (cysteine-rich 61/CCN1),CTGF (connective tissue growth factor/CCN2), and NOV (nephroblastomaoverexpressed/CCN3). These proteins regulate a variety of cellularfunctions including cell adhesion, migration, apoptosis, survival andgene expression. TGF-β strongly upregulates the CCN2 expression whichacts synergistically as a co-factor with TGF-β and seems to be involvedin pericyte activation, a process which appears to be essential infibrosis (Leask ibid). CCN2 is overexpressed in fibrotic tissue,including pulmonary tissue and is also found in the plasma of patientswith systemic sclerosis (scleroderma). Also, knock down of CCN2expression through use of antisense oligonucleotides (ASO) reducedchemical-induced liver fibrosis, ureteral obstruction-induced renalfibrosis, fibrotic scarring in cutaneous wounds, and renal interstitialfibrogenesis following partial nephrectomy (Jun and Lau (2013) Nat RevDrug Discov. 10(12): 945-963). In addition to its pro-fibrotic role,CCN2 may be important in cancer, especially in metastasis. It maypromote tumor growth by inducing angiogenesis, and high levels of CCN2in breast cancer cells is a marker of bone metastasis potential (Jun andLau, ibid). Experimental models that knock down CCN2 expression invarious models of fibrosis, cancer, cardiovascular disease andretinopathy through the use of CCN2 modulating compounds such asmonoclonal antibodies or inhibitory RNAs have shown impact of clinicalprogression of a number of these diseases. (Jun and Lau ibid). Thus, themethods and compositions of the invention can be used to prevent ortreat fibrosis, cancer, vascular disease and retinopathy by decreasingexpression of CCN2 by targeted degradation of CCN2 using the dTAGinsertion strategy described herein.

In other embodiments, the target(s) are involved in an autoimmunedisease. Autoimmune diseases as a class are common, and affect more than23 million people in the United States alone. There are severaldifferent kinds with many different levels of severity and prognoses.Generally, they are characterized by the production of auto-antibodiesagainst various self-antigens leading to an immune response againstone's own body. Autoimmune disease of the gut can lead to conditionssuch as ulcerative colitis and inflammatory/irritable bowel disease(e.g., Crohn's disease). The cell surface glycoprotein intercellularadhesion molecule 1 (ICAM-1) is expressed on endothelial cells andupregulated in inflammatory states, serving as a binding protein forleukocytes during transmigration into tissues. Specific ICAM-1 alleleshave been found to be associated with Crohn's disease (e.g. K469Eallele, exon 6) or with ulcerative colitis (e.g. G241R, exon 4) and maypreferentially participate in the chronic inflammatory induction foundin these diseases (Braun et at (2001) Clin Immunol. 101(3):357-60).Knock out of ICAM in mouse models of vascular and diabetic disease havedemonstrated the usefulness of this therapeutic approach (see Bourdillonet at (2000) Ather Throm Vasc Bio 20:2630 and Okada et at (2003)Diabetes 52:2586, respectively). Thus, the methods and compositions ofthis invention may be used for the general reduction of ICAM expressionin inflammatory diseases by targeted degradation of ICAM using the dTAGinsertion strategy described herein.

Another common disease that has been more recently recognized as anautoimmune disease is diabetes. Glucagon, a peptide hormone released bythe α-cell of pancreatic islets, plays a key role in regulating hepaticglucose production and has a profound hyperglycemic effect. In addition,glucagon activates multiple enzymes required for gluconeogenesis,especially the enzyme system for converting pyruvate tophosphoenolpyruvate, the rate-limiting step in gluconeogenesis. It hasbeen proposed that hyperglucagonemia is a causal factor in thepathogenesis of diabetes based on the following observations: 1)diabetic hyperglycemia, from animal to human studies, is consistentlyaccompanied by relative or absolute hyperglucagonemia; 2) infusion ofsomatostatin inhibits endogenous glucagon release, which in turn reducesblood glucose levels in dogs with diabetes induced by alloxan ordiazoxide; and 3) chronic glucagon infusion leads to hepatic insulinresistance in humans (see Liang et at (2004) Diabetes 53(2):410). Theglucagon receptor (encoded by the GCGR gene) is expressed predominantlyin the liver, and treatment of diabetic (db/db) mice with antisense RNAtargeting the glucagon receptor causes a significant reduction in serumglucose levels, triglycerides and fatty acids in comparison withcontrols (Liang et al, ibid). Similarly, glucocorticoids (GCs) increasehepatic gluconeogenesis and play an important role in the regulation ofhepatic glucose output. In db/db mice, a reduction in glucocorticoidreceptor (GCCR) expression through the use of targeted antisense RNAscaused ˜40% decrease in fed and fasted glucose levels and 50% reductionin plasma triglycerides (see Watts et at (2005) Diabetes 54(6):1846).Thus, the methods and compositions of the invention may be used toprevent or treat diabetes through targeting the glucagon receptor and/orthe glucocorticoid receptor by decreasing expression of the glucagonreceptor and/or glucocorticoid receptor by targeted degradation usingthe dTAG insertion strategy described herein.

Another potential target in type 2, insulin resistant diabetes isprotein tyrosine phosphatase 1B (PTP-1B). Insulin resistance is definedas the diminished ability of cells to respond to insulin in terms ofglucose uptake and utilization in tissues. One of the most importantphosphatases regulating insulin signaling is the PTP-1B which inhibitsinsulin receptor and insulin receptor substrate 1 by directdephosphorylation. Mice that are PTP-1B −/− (mutated at both alleles)are hypersensitive to insulin and resistant to weight gain on high fatdiets (see Fernandez-Ruiz et at (2014) PLoS One 9(2):e90344). Thus thistarget may be useful for both diabetes treatment and obesity. Developinginhibitory small molecules specific for this enzyme is problematicbecause of the highly conserved active site pocket, but antisenseoligonucleotides directed PTP-1B has been shown to reduce PTP-1B mRNAexpression in liver and adipose tissues by about 50% and to produceglucose lowering effects in hyperglycemic, insulin-resistant ob/ob anddb/db mice, experiments that were repeated in non-human primates (seeSwarbrick et at (2009) Endocrin 150:1670). Thus, the methods andcompositions of the invention can be used to target the PTP-1B bytargeted degradation of PTP-1B using the dTAG insertion strategydescribed herein, leading to increased insulin sensitivity.

A high risk factor for developing type diabetes insulin resistantdiabetes is obesity. Worldwide, more than 1 billion people are estimatedto be overweight (body mass index (BMI)≥25 kg/m2, and more than 300million of these are considered obese (BMI≥30 kg/m2), meaning thatobesity is one of the greatest threats to public health today (Lagerrosand Rossner (2013) Ther Adv Gastroenterol 6(1):77). Obesity is highlyassociated with co-morbidities such as insulin resistant type IIdiabetes, dyslipidemia, hypertension and cardiovascular disease.Treatment of obesity typically starts with modification of diet andexercise, but often with a decrease in caloric consumption, a paralleland confounding decrease in energy expenditure by the body is observed(Yu et al, (2013) PLoS One 8(7):e66923). Fibroblast growth factorreceptor 4 (FGFR4) has been shown to have an anti-obesity effect inmouse obesity models. FGFR4 is mainly expressed in the liver, and it andits ligand FGF19 (in humans) regulate bile acid metabolism. FGFR4/FGF19regulate the expression of cholesterol 7 alpha-hydroxylase and itsactivity. In addition, FGFR4 and FGF19 seem to be involved in lipid,carbohydrate or energy metabolism. Hepatic FGFR4 expression is decreasedby fasting, and increased by insulin. FGFR4 null mice also show changesin lipid profiles in comparison with wild type mice in response todifferent nutritional conditions. Treatment of obese mice with FGF 19increased metabolic rate and improved adiposity, liver steatosis,insulin sensitivity and plasma lipid levels, and also inhibited hepaticfatty acid synthesis and gluconeogenesis while increasing glycogensynthesis. Anti-sense reduction of FGFR4 in obese mice also lead toreduced body weight and adiposity, improvement in insulin sensitivityand liver steatosis, and increased plasma FGF15 (the mouse equivalent ofFGF19) levels without any overt toxicity (Yu et al, ibid). Thus, themethods and compositions of the invention can be used to treat obesityby reducing the expression of FGFR4 by targeted degradation using thedTAG insertion strategy described herein.

Multiple sclerosis (MS) is a chronic, disabling, autoimmune disease ofthe central nervous system that is characterized by inflammation,demyelination and axonal destruction. The flare ups associated withrelapsing MS (occurring in 85-95 percent of patients) are thought to betied to the entry of activated lymphocytes into the brain. Currentlyavailable treatments are only able to inhibit the rate of relapses byabout 30%. Inflammatory responses induce the expression of vascularadhesion molecule-1 (VCAM-1) on the endothelium of the vasculature, andthe adhesion of the lymphocytes to VCAM-1 is a necessary step that thenallows the activated cells to pass through into the brain. VCAM-1adherence by the lymphocytes is mediated by binding of very lateantigen-4 (VLA-4, also known as 4p131 integrin) on the surface of theactivated lymphocyte (Wolf et at (2013) PLos One 8(3): e58438).Disruption of this interaction has been the idea behind the therapeuticuse of anti-VLA-4 specific antibodies and small molecule antagonists(Wolf et al, ibid). Thus, the materials and methods of the invention canbe used to target VCAM-1 or VLA-4 expression by targeted degradationusing the dTAG insertion strategy described herein.

Another disease of interest is Cushing's disease/syndrome (CS). In thisdisease, patients have elevated serum levels of glucocorticoid due toincreased expression by the adrenal gland. CS is an uncommon conditionwith an incidence rate between 1.8 and 2.4 patients/million per year.The most common cause of endogenous CS is an ACTH-producing pituitaryadenoma, seen in 70% of patients with CS. Cortisol-producing adrenaladenomas and ectopic ACTH-producing tumors are less common, eachaccounting for ^(˜)10-15% of cases. The first-line treatment forpatients with pituitary derived CS is transsphenoidal pituitary surgery(TSS) and unilateral adrenalectomy for cortisol-producing adrenaladenoma. Unilateral adrenalectomy is curative in almost all patientswith cortisol-producing adrenal adenoma and permanent adrenalinsufficiency is rare. Conversely, hypopituitarism is common after TSS,with a range between 13 and 81% (see Ragnarsson and Johannsson (2013)Eur J Endocrin 169:139). In some patients however, surgical resection isnot successful and so pharmacological treatment is indicated. Oneapproach is to inhibit the activity of the hypercortisolemia bytargeting the glucocorticoid receptor (GCCR), for example, usingMifepristone (also known as RU 486), a GCCR antagonist (see Johanssenand Allolio (2007) Eur J Endocrin 157:561). However, RU 486 has severalother activities (most notably, induction of an abortion in pregnantpatients). Thus, the methods and compositions of the invention may beused to target the GCCR by decreasing expression by targeted degradationusing the dTAG insertion strategy described herein.

Transthyretin Amyloidoses (TTRA) is one of several degenerative diseasessuspected to be linked to misfolded and aggregated protein (amyloids).Transthyretin (TTR) is a tetramer produced in the liver and secretedinto the bloodstream that serves to transport holo-retinal bindingprotein. However, upon conformational changes, it becomes amyloidogenic.Partial unfolding exposes stretches of hydrophobic residues in anextended conformation that efficiently misassemble into largelyunstructured spherical aggregates that ultimately before cross-β sheetamyloid structures (see Johnson et at (2012) J Mol Biol 421(2-3): 183).TTRA can occur in patients in both sporadic and autosomal dominantinherited forms which include familial amyloid polyneuropathy (FAP) andfamilial amyloid cardiomyopathy (FAC). These inherited forms are usuallyearlier onset and relate to over 100 point mutations described in theTTR gene. Generally, the more destabilizing of the protein that themutation is, the more likely it is to have some amount of amyloidpathology. The amyloid formed causes selective destruction of cardiactissue in FAC or peripheral and central nervous tissue in FAP. Some newtherapeutic strategies for treating these diseases such as inhibitoryRNA strategies center on trying to decrease the amount of TTR todecrease the aggregation potential of the protein (Johnson et al, ibid).Thus the methods and compositions of the invention can be used to targetTTR in an effort to reduce the quantity of the pathological forms of theTTR protein and/or to decrease TTR concentration in general by targeteddegradation using the dTAG insertion strategy described herein.

Muscular diseases can also be approached using the methods of theinvention. Spinal muscular atrophy is an autosomal recessive diseasecaused by a mutation in the SMN1 gene which encodes the ‘survival ofmotor neuron’ (SMN) protein and is characterized by general musclewasting and movement impairment. The SMN protein is involved in theassembly of components of the spliceosome machinery, and several defectsin the SMN1 gene are associated with splicing defects that cause exon 7of the mature mRNA to be specifically excluded. These defects areespecially prevalent in spinal motor neurons, and can cause spinalmuscular atrophy. The severity of SMN1 defects can be modified by aparalogue of SMN1 known as SMN2. The SMN2 gene sequence differs fromSMN1 in only a few single nucleotide polymorphisms in exons 7 and 8 andseveral others in the intronic sequences. Thus the methods andcompositions of the invention can be used to target SMN1 in an effort toreduce the quantity of the pathological forms of the SMN1 protein and/orto decrease SMN1 concentration in general by targeted degradation usingthe dTAG insertion strategy described herein.

Dysregulation of the secretion of growth hormone (GH) can lead to acondition known as acromegaly, a disorder of disproportionate skeletal,tissue, and organ growth which first becomes evident at about 40 yearsof age (Roberts and Katznelson (2006) US Endocrine Disease: 71). Itoccurs an annual incidence of approximately 5 cases per million, anddiagnosis requires a determination of dysregulation of GH secretion andelevated IGF 1 levels. The inability to suppress GH secretion during the2 hours post an oral glucose load is generally used for diagnosis ofacromegaly. Normal regulation of GH secretion is carried out by thepituitary gland. Hypothalamic GH-releasing hormone (GHRH), ghrelin andsomatostatin regulate GH production by anterior pituitary somatotrophcells. The gene encoding the GH receptor or GHR is widely expressed andwhen a GH molecule interacts with a GHR dimer, signal proceeds viaJAK2-dependent and independent intracellular signal transductionpathways (see Melmed (2009) J Clin Invest 119(11):3189). Circulating GHstimulates hepatic secretion of insulin-like growth factor-1 (IGF-1).Acromegaly occurs when benign pituitary tumors cause an increase in GHsecretion and thus in IGF-1 secretion. One GHR mutation that is tied toacromegaly has an in-frame deletion in exon 3 that causes a deletion of22 amino acids in the protein. This mutated receptor, known as d3-GHR,results in enhanced GH responsiveness. Current therapies focus on thenormalization of GH and IGF-1 levels, often through surgical removal ofthe pituitary tumors. Since secretion of IGF-1 is induced by GH,targeting of the GHR is an attractive target for the methods andcompositions of the invention. Thus, the methods and compositions of theinvention may be used to target GHR by decreasing expression by targeteddegradation using the dTAG insertion strategy described herein.

Another disease associated with muscle wasting is myotonic dystrophy,which is a chronic disease characterized by muscle wasting, cataracts,heart conduction defects, endocrine changes, multiorgan damage andmyotonia (prolonged muscle contraction following voluntary contraction).Myotonic dystrophy occurs at an incidence rate of approximately 13 per100,000 people, and there are two forms of the disease, MyotonicDystrophy Type 1 (also called Steinert's disease, MMD1 or DM1, and isthe most common) and Myotonic Dystrophy Type 2 (MMD2 or DM2). Both areinherited autosomal dominant diseases caused by abnormal expansions inthe 3′ non-coding regions of two genes (CTG in the DMPK gene (encodingdystrophia myotonica protein kinase) for type 1, and CCTG in the ZNF9gene (encoding cellular nucleic acid-binding protein) in type 2) and DM1is the most common form of muscular dystrophy in adults. These mutationsresult in toxic intranuclear accumulation of the mutant transcripts inRNA inclusions or foci (see Caillet-Boudin et al, (2014) Front. Mol.Neurosci doi: 10.3389). Type 1 patients have CTG copy numbers greaterthan 50 and have variable phenotypes, ranging from asymptomatic tosevere. Antisense RNA techniques have been used to cause the specificdestruction of the mutant DMPK transcripts in vitro which caused noeffect on the proliferation rate of DM1 myoblasts but restored theirdifferentiation (Furling et at (2003) Gene Therapy 10:795). Thus, themethods and compositions of the invention can be used to target thedystrophia myotonica protein kinas or cellular nucleic acid bindingprotein by targeted degradation using the dTAG insertion strategydescribed herein.

Chronic pain is a major health concern affecting 80 million Americans atsome time in their lives with significant associated morbidity andeffects on individual quality of life. Chronic pain can result from avariety of inflammatory and nerve damaging events that include cancer,infectious diseases, autoimmune-related syndromes and surgery.Voltage-gated sodium channels (VGSCs) are fundamental in regulating theexcitability of neurons and overexpression of these channels can produceabnormal spontaneous firing patterns which underpin chronic pain. Thereare at least nine different VGSC subtypes in the nervous system, andeach subtype can be functionally classified as eithertetrodotoxin-sensitive or tetrodotoxin-resistant. Neuronal sodiumchannel subtypes including Nav1.3, Nav1.7, Nav1.8, and Nav1.9 have beenimplicated in the processing of nociceptive information. The VGSC Nav1.8is a tetrodotoxin-resistant sodium channel with a distributionrestricted to primary afferent neurons and the majority ofNav1.8-containing afferents transmit nociceptive signals to painprocessing areas of the spinal cord. Changes in the expression,trafficking and redistribution of Nav1.8 (encoded by PN3) followinginflammation or nerve injury are thought to be a major contributor tothe sensitization of afferent nerves and the generation of pain (seeSchuelert and McDougall (2012) Arthritis Res Ther 14:R5). Rodent modelsof osteoarthritis have demonstrated that inhibition of Nav1.8 channelson peripheral nerves, with synaptic connections in the spinal cord, is apromising treatment of nociceptive sensory processing and could behelpful to achieve more pronounced and longer lasting analgesia. Thus,the methods and compositions of the invention can be used to treatchronic pain by decreasing localized expression of NAV1.8 by targeteddegradation using the dTAG insertion strategy described herein.

Cancer may also be targeted as described herein. Cancer is a genericterm used to describe a number of specific diseases that are united by alack of cellular growth regulation. Since there are so many forms,involving a myriad of different cell types, there are also numerousspecific gene targets that are involved in cancer. For example, theclusterin protein (also known as apolipoprotein J), encoded by the CLUgene, is a heterodimeric protein assembled following the proteolyticcleavage into the two chains of the primary polypeptide CLU geneproduct. In recent years, it has been found that there are two forms ofclusterin, a secretory and heavily glycosylated form (sCLU) and anuclear form (nCLU), where nCLU is first synthesized as a pre nuclearform (pnCLU) that is found in the cell cytoplasm. The differencesbetween the two CLU forms are tied to alternative splicing of the CLUmessage and the selection of the starting ATG during messagetranslation. The translation of sCLU utilized the first AUG in the fulllength CLU mRNA whereas the translation of pnCLU is initiated from asecond in-frame AUG following the splice-dependent removal of thetranscribed leader section and Exon 1 from the full length mRNA. ThesCLU form appears to promote cell survival while the nCLU form isassociated with apoptosis. Overexpression of the sCLU form of theprotein has been found in many tumor types, including prostate, skin,pancreatic, breast, lung, and colon tumors, as well as oesophagealsquamous cell carcinoma and neuroblastoma. In addition, the progressionof some cancer types towards high grade and metastatic forms leads to anelevation of sCLU levels (Shannan et at (2006) Cell Death Dif 13: 12).Use of specific antisense oligonucleotides (ASO) designed to causesilencing sCLU expression in combination with standard treatments hasbeen carried out in Phase I studies of breast and prostate cancer, withan increase in apoptosis observed only in the patients that receivedboth the ASO and the standard therapeutic agent (Shannan ibid). Thus,the methods and compositions of the invention can be used to treatcancers marked with an increase in sCLU expression by targeteddegradation using the dTAG insertion strategy described herein.

Another protein that appears to have an oncogenic role is eukaryotictranslation initiation factor 4E (eIF-4E). eIF3-4E binds to the M7GpppNcap (where N is any nucleotide) of a eukaryotic mRNA and is the ratelimiting member for the formation of the eIF-4F complex. elF-4E normallycomplexes with eIF-4G in the eIF-4F complex, and under normalphysiologic conditions, the availability of eIF-4E is negativelyregulated by the binding of a family of inhibitory proteins known as4E-BPs which act to sequester eIF-4E from eIF-4G. Since eIF-4E isexpressed normally at low levels, mRNAs compete for the available eIF-4Eto be translated. mRNAs with short, unstructured 5′ UTRs are thought tobe more competitive for translation since they are less dependent on theunwinding activity found in the eIF-4F complex. mRNAs that are highlystructural then are more dependent on eIF-4E binding for translation,and thus when eIF3-4E is overexpressed, these mRNAs are more easilytranslated. Growth-promoting gene products such as cyclin Dl, VEGF,c-myc, FGF2, heparanase, ODC and MMP9 have these complex 5′ UTRs (Mamaneet at (2004) Oncogene 23:3172, Fischer (2009) Cell Cycle 8(16):2535).Additionally, eIF-4E may serve a role in modification of the nuclearpore complex and cause an increase in translocation of these same mRNAsinto the cytoplasm (Culjikovic-Kraljacic et at (2012) Cell Reports 2 p.207). eIF-4E has been implicated in oncogenic cellular transformationand is overexpressed in several cancer types including acute myeloidleukemia, colon, breast, bladder, lung, prostate, gastrointestinaltract, head and neck cancers, Hodgkin's lymphoma and neuroblastoma andelevated levels are associated with increasing grade of disease.Targeting of eIF-4E has been attempted by several different approaches,including overexpression of 4E-BPs and peptides derived there from, thedevelopment of small molecule inhibitors to prevent eIF-4E:eIFGinteraction, and antisense oligos (ASO) specific for eIF-4E (Jia et at(2012) Med Res Rev 00, No. 00:1-29). ASO administration has demonstrateda knock down of eIF-4E expression in tumor cells in vitro, and inxenograft tumors in mouse models in vivo. Expression levels of eIF-4Ewere decreased by 80% in these mouse models without any decrease inoverall protein translation and without any obvious toxicity, whileincreasing chemosensitivity to chemotherapeutic agents, increasingcancer cell apoptosis and suppressing tumor growth (Jia ibid). Thus, themethods and compositions of the invention may be used for the treatmentor prevention of various cancers. Expression of eIF-4F can be modulatedby degradation using the dTAG insertion strategy described herein.

Vascular endothelial receptor (VEGF), acting via its receptor VEGFR hasa role in normal development, and also in the development ofpathological angiogenesis in cancer. In humans, there are five distinctVEGF family members: VEGF-A (also known as VEGF); placenta growth factor(PIGF), VEGF-B, VEGF-C and VEGF-D. VEGF-A also has three commonsubtypes: VEGF-121. VEGF-165 and VEGF-189. The various VEGFs havediffering roles in angiogenesis with VEGF-A primarily being involved innormal angiogenesis and also in tumor growth and metastasis, whileVEGF-C and VEGF-D are involved in normal lymphangiogenesis and inmalignant lymph node metastasis. In addition, the VEGF-A subtypes mayalso have specific growth promoting activity in hormone responsivetumors. Based on this knowledge, a number of antibodies and smallmolecule kinase inhibitors which suppress the VEGF-VEGFR interactiondirectly or the signal transduction pathways activated by theinteraction. However, these therapeutics often have significant andpotentially troublesome side effect profiles, such that active researchis occurring to develop inhibitors with increased specificity (Shibuya,(2014) Biomol Ther 11(1): 1-9). Thus, the methods and compositions ofthe invention may be used to prevent or treat cancer in a subject bytargeting specific VEGF proteins by degradation using the dTAG insertionstrategy described herein.

Another protein that plays a role in several cancers is kinesin spindleprotein (KSP), encoded by the KIF11 gene. The most successfulanti-cancer therapies currently in use target microtubules where theseagents have been used for the treatment of breast, lung, ovarian,bladder, and head and neck cancers. Microtubules are part of the mitoticspindle, and thus targeting them is successful in inhibiting rapidlydividing cancer cells, but microtubules are also part of thecytoskeleton, such that treatment with these agents also is associatedwith serious side effects. Kinesin, specifically kinesin spindleprotein, is a motor protein that binds to spindle fibers and serves toforce the spindle fibers apart during chromosome segregation in celldivision. Thus, targeting KSP using a KSP-specific anti-mitotic agentwill only target dividing cells, and might have fewer side effects.Agents that deplete KSP selectively lead to cell cycle arrest inmitosis, which after a prolonged period, leads to apoptosis. KSP is alsoabundant in dividing tissues, and is highly expressed in tumors of thebreast, colon, lung, ovary and uterus (Sarli and Giannis, (2008) ClinCancer Res 14:7583). In addition, clinical trials are underway using RNAinterference targeted to KSP and VEGF simultaneously in cancer patientswith liver involvement (Tabernero et al, (2013) Cancer Discovery 3:406).Thus, the methods and compositions of the invention may be used to treator prevent cancers by targeted degradation of the kinesin spindleprotein (KSP) using the dTAG insertion strategy described herein.

Heat shock protein 27 (HSP 27, also known as heat shock protein beta-1or HSPB1) is another protein that is implicated in cancer. HSP 27,encoded by the HSPB1 gene, is a heat shock protein that was initiallycharacterized in response to heat shock as a small chaperonin thatfacilitates proper refolding of damaged proteins. However, ongoinginvestigation revealed that it also is involved in responses to cellularstress conditions such as oxidative stress, and chemical stress, appearsto have anti-apoptotic activity, and is able to regulate actincytoskeletal dynamics during heat shock and other stress conditions(Vidyasagar et at (2012) Fibrogen Tis Rep 5(7)). In addition,suppression of HSP 27 may play a role in long term dormancy of cancersas research has revealed that HSP 27 is upregulated in angiogenic breastcancer cells, and suppression of HSP 27 in vivo leads to long term tumordormancy (Straume et at (2012) Proc Natl Acad Sci USA 109(22):8699-8704). Increased expression of heat shock proteins in tumor cellsis related to loss of p53 functions and to the upregulation ofproto-oncogenes such as c-myc. HSP 27's anti-apoptotic activity protectstumor cells and also has been shown to be associated with chemotherapyresistance in breast cancer and leukemia (Vidysagar ibid). Thus, HSP 27may be a suitable target for cancer therapeutics, where inhibitors ofthe protein may be used in combination with known chemotherapies toenhance their activities. The HSP 27 inhibitor quercetin has been shownto significantly reduce tumor volumes in vivo when combined withtraditional chemotherapeutic agents in comparison with the agents alone.In addition, HSP 27 inhibitory ASOs are currently be evaluated inclinical studies in lung, ovarian, breast and pancreatic cancers(Vidyasagar, ibid). Thus, the methods and compositions of the inventionmay be used to treat cancers by inhibition of HSP 27 expression throughtargeted degradation of HSP 27 using the dTAG insertion strategydescribed herein.

Several kinases have been the target of research into anti-cancertherapeutics since they are often key regulators of cell growth.However, downstream in the signaling pathway, the effect of mutantkinases is often seen in the upregulation of the Signal Transduction andActivator of Transcription 3 protein, or Stat3, encoded by the STAT3gene. Additionally, it appears that both Hepatitis B and C activateStat3, and both are associated with the development of hepatic cancer.Thus it may be that the HepB and HepC viruses subvert Stat3 signalingpathways and promote hepatocyte transformation (Li et al, (2006) ClinCancer Res 12(23):7140).

RAS proteins are a family of proteins that play a role in celldifferentiation, proliferation, and survival. Various members of the RASprotein family have been implicated in cancer as aberrant RAS signalinghas been found to play a role in approximately 30% of all cancers. TheKRAS protein (also known as V-Ki-ras2 Kirsten rat sarcoma viral oncogenehomolog) is a GTPase that performs an essential finction in normaltissue signaling KRAS is an attractive cancer target, as frequent pointmutations in the KRAS gene render the protein constitutively active.Thus, KRAS may be a suitable target for cancer therapeutics, where smallmolecules targeting the function of the KRAS protein may be used fortherapeutic advantages, including in combination with knownchemotherapies to enhance their activities. In one embodiment, themethods and compositions of the invention may be used to treat cancersby modulation of KRAS expression through targeted degradation of KRASusing the dTAG insertion strategy described herein.

All the various Stat proteins are transcription factors that primarilymediate signaling from cytokine and growth factor receptors. Forexample, IL6 and IL11 bind to their respective receptor subunits andtrigger homodimerization of gp130, the transmembrane receptor thattriggers Stat3 activation. Following activation via phosphorylation ofthe growth factor receptors, Stat3 proteins dimerize and traverse intothe nucleus and bind to DNA in a sequence specific manner, up regulatingmany genes that are involved in cell proliferation. Tumor cells ofvarious types often have kinase mutations that lead to overexpression ofStat3 so a decrease in Stat3 expression has the potential to bebeneficial in cancers of multiple origins without regard to eachspecific mutant kinase (Jarnicki et at (2010) Cell Div 5:14). Stat3contributes to malignancy by several mechanisms. It inhibits apoptosisby upregulating the pro-survival/anti-apototic Bcl2 proteins andpromotes proliferation primarily by stimulating expression of cyclinB1,cdc2, c-myc, VEGF, H1F1α and cyclin D1 as well as through its repressionof the cell cycle inhibitor p21. Stat3 also promotes tumor metastasisthrough the induction of extracellular matrix-degradingmetalloproteinases including MMP-2 and MMP-9. In normal physiologicalstates, Stat3 functioning is inhibited by the transcriptional inhibitorSocs3, which is normally induced by Stat3 to maintain growth balance inthe cell. However, in a malignant cell, Stat3 overexpression canovercome Socs3 inhibition. Thus, the methods and compositions of theinvention can be used to inhibit Stat3 functioning and prevent or treatcancer by targeted degradation of Stat3 using the dTAG insertionstrategy described herein.

Prostate cancer (PCa) is an androgen-dependent disease that remains oneof the leading causes of death in the United States, and is the leadingcause of death from cancer in men. While several studies have been donethat suggest that up to 42% of prostate cancer cases have a genetic link(Mazaris and Tsiotras (2013) Nephro Urol Mon 5(3):792-800), severaltypes of inheritance patterns have been observed (e.g. X-linked,autosomal dominant, autosomal recessive) suggesting that there is notone sole gene or gene mutation that leads to inheritance of PCa. Thiscancer is dependent upon the activity of the androgen receptor forgrowth and progression (Mahmoud et at (2013) PLoS One 8(10): e78479).Typically, PCa can be a slow to progress disease that can be treatedusing fairly conservative approaches, but in about 25-30% of the cases,the cancer can be an aggressive one leading to patient death. In thecase of metastatic disease 70-80% of patients respond initially toandrogen-deprivation therapy but in later stages, the tumor becomeshormone refractory and more aggressive, leading to a worsening prognosis(Mazaris and Tsiotras ibid). Hormone refractory PCa is not dependent oncirculating androgen, but rather is driven by inappropriate activationof the androgen receptor (AR, encoded by the AR gene) through suchmechanisms as AR amplification, deregulation of growth factors, andco-amplification of AR cofactors. Additionally, mutations in the ARligand binding domain can cause the AR to be supersensitive to very lowcirculating androgen levels or to be sensitive to an expanded set ofligands such as estrogens, progestins, adrenyl steroids andantiandrogens. Tumor cells that have undergone these types of mutationsin the AR ligand binding domain may no longer be sensitive toanti-androgen therapies despite the reliance of the cancer on theactivity of the AR. Normally the AR is present in the cytoplasm and isbound by heat shock proteins to prevent its activation. Upon exposure toandrogen, the receptor is able to dimerize and travel into the cellnucleus to promote expression of several growth related genes. Thus themethods and compositions of the invention may be used to treat PCa atall stages by targeting degradation of the androgen receptor using thedTAG insertion strategy described herein.

C. Genomic In-Frame Insertion of dTAGs

As described above, the methods of the present invention are based onthe genomic insertion of a dTAG in-frame with a gene expressing anendogenous protein of interest. As contemplated herein, the 5′- or 3′in-frame insertion of a nucleic acid sequence encoding a dTAG results,upon expression of the resultant nucleic acid sequence, in an endogenousprotein-dTAG hybrid protein that can be targeted for degradation by theadministration of a specific heterobifunctional compound.

In-frame insertion of the nucleic acid sequence encoding the dTAG can beperformed or achieved by any known and effective genomic editingprocesses. In one aspect, the present invention utilizes the CRISPR-Cas9system to produce knock-in endogenous protein-dTAG fusion proteins thatare produced from the endogenous locus and are readily degraded in aligand-dependent, reversible, and dose-responsive, fashion. In certainembodiments, the CRISPR-Cas9 system is employed in order to insert anexpression cassette for dTAG present in a homologous recombination (HR)“donor” sequence with the dTAG nucleic acid sequence serving as a“donor” sequence inserted into the genomic locus of a protein ofinterest during homologous recombination following CRISPR-Casendonucleation. The HR targeting vector contains homology arms at the 5′and 3′end of the expression cassette homologous to the genomic DNAsurrounding the targeting gene of interest locus. By fusing the nucleicacid sequence encoding the dTAG in frame with the target gene ofinterest, the resulting fusion protein contains a dTAG that is targetedby a heterobifunctional compound.

The present invention provides for insertion of an exogenous dTAGsequence (also called a “donor sequence” or “donor” or “transgene”) inframe with the target gene of interest, and the resulting fusion proteincontains a dTAG that is targeted by a heterobifunctional compound. Itwill be readily apparent that the donor sequence need not be identicalto the genomic sequence where it is placed. A donor sequence can containa non-homologous sequence flanked by two regions of homology to allowfor efficient HR at the location of interest. Additionally, donorsequences can comprise a vector molecule containing sequences that arenot homologous to the region of interest in cellular chromatin. A donormolecule can contain several, discontinuous regions of homology tocellular chromatin. For example, for targeted insertion of sequences notnormally present in a region of interest, for example, the dTAGs of thepresent invention, said sequences can be present in a donor nucleic acidmolecule and flanked by regions of homology to sequence in the region ofinterest. Alternatively, a donor molecule may be integrated into acleaved target locus via non-homologous end joining (NHEJ) mechanisms.See, e.g., U.S. 2011/0207221 and U.S. 2013/0326645, incorporated hereinby reference.

The donor dTAG encoding sequence for insertion can be DNA or RNA,single-stranded and/or double-stranded and can be introduced into a cellin linear or circular form. See, e.g., U.S. 2010/0047805, U.S.2011/0281361, and 2011/0207221, incorporated herein by reference. Thedonor sequence may be introduced into the cell in circular or linearform. If introduced in linear form, the ends of the donor sequence canbe protected (e.g., from exonucleolytic degradation) by methods known tothose of skill in the art. For example, one or more dideoxynucleotideresidues are added to the 3′ terminus of a linear molecule and/orself-complementary oligonucleotides are ligated to one or both ends.See, for example, Chang et al. Proc. Natl. Acad. Sci. 84,(1987):4959-4963 and Nehls et al. Science, 272, (1996):886-889.Additional methods for protecting exogenous polynucleotides fromdegradation include, but are not limited to, addition of terminal aminogroup(s) and the use of modified internucleotide linkages such as, forexample, phosphorothioates, phosphoramidates, and O-methyl ribose ordeoxyribose residues.

The donor polynucleotide encoding a dTAG can be introduced into a cellas part of a vector molecule having additional sequences such as, forexample, CRISPR-Cas sequences, replication origins, promoters and genesencoding antibiotic resistance. Moreover, donor polynucleotides can beintroduced as naked nucleic acid, as nucleic acid complexed with anagent such as a liposome or poloxamer, or can be delivered by viruses(e.g., adenovirus, AAV, herpesvirus, retrovirus, lentivirus andintegrase defective lentivirus (IDLV)).

The present invention takes advantage of well-characterized insertionstrategies, for example the CRISPR-Cas9 system. In general, the “CRISPRsystem” refers collectively to transcripts and other elements involvedin the expression of or directing the activity of CRISPR-associated(“Cas”) genes, including sequences encoding a Cas gene, a tracr(trans-activating CRISPR) sequence (e.g. tracrRNA or an active partialtracrRNA), a tracr-mate sequence (encompassing a “direct repeat” and atracrRNA-processed partial direct repeat in the context of an endogenousCRISPR system), a guide sequence (also referred to as a “spacer” in thecontext of an endogenous CRISPR system), and/or other sequences andtranscripts from a CRISPR locus. (See, e.g., Ruan, J. et al. “Highlyefficient CRISPR/Cas9-mediated transgene knockin at the H11 locus inpigs.” Sci. Rep. 5, (2015):14253; and Park A, Won S T, Pentecost M,Bartkowski W, and Lee B “CRISPR/Cas9 Allows Efficient and CompleteKnock-In of a Destabilization Domain-Tagged Essential Protein in a HumanCell Line, Allowing Rapid Knockdown of Protein Function.” PLoS ONE 9(4),(2014): e95101, both incorporated herein by reference).

The Cas nuclease is a well-known molecule. For example, the proteinsequence encoded by the Cas-9 nuclease gene may be found in theSwissProt database under accession number

Q99ZW2-(SEQ. ID. NO.: 52):MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFEIRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIH QSITGLYETRIDLSQLGGD.

In some embodiments, the CRISPR/Cas nuclease or CRISPR/Cas nucleasesystem includes a non-coding RNA molecule (guide) RNA, whichsequence-specifically binds to DNA, and a Cas protein (e.g., Cas9), withnuclease functionality (e.g., two nuclease domains). Further included isthe donor nucleotide encoding a dTAG for in-frame insertion into thegenomic locus of the protein of interest.

In some embodiments, one or more elements of a CRISPR system is derivedfrom a type I, type II, or type III CRISPR system. In some embodiments,one or more elements of a CRISPR system is derived from a particularorganism comprising an endogenous CRISPR system, such as Streptococcuspyogenes.

In some embodiments, a Cas nuclease and gRNA (including a fusion ofcrRNA specific for the target sequence and fixed tracrRNA), and a donorsequence encoding a dTAG are introduced into the cell. In general,target sites at the 5′ end of the gRNA target the Cas nuclease to thetarget site, e.g., the gene, using complementary base pairing. In someembodiments, the target site is selected based on its locationimmediately 5′ of a protospacer adjacent motif (PAM) sequence, such astypically NGG, or NAG. In this respect, the gRNA is targeted to thedesired sequence by modifying the first 20 nucleotides of the guide RNAto correspond to the target DNA sequence.

In some embodiments, the CRISPR system induces DSBs at the target site,followed by homologous recombination of the donor sequence encoding adTAG into the genomic locus of a protein of interest, as discussedherein. In other embodiments, Cas9 variants, deemed “nickases” are usedto nick a single strand at the target site. In some aspects, pairednickases are used, e.g., to improve specificity, each directed by a pairof different gRNAs targeting sequences such that upon introduction ofthe nicks simultaneously, a 5′ overhang is introduced.

In general, a CRISPR system is characterized by elements that promotethe formation of a CRISPR complex at the site of a target sequence.Typically, in the context of formation of a CRISPR complex, “targetsequence” generally refers to a sequence to which a guide sequence isdesigned to have complementarity, where hybridization between the targetsequence and a guide sequence promotes the formation of a CRISPRcomplex, and wherein insertion of the donor sequence encoding a dTAG isto take place. Full complementarity is not necessarily required,provided there is sufficient complementarity to cause hybridization andpromote formation of a CRISPR complex.

Typically, in the context of an endogenous CRISPR system, formation ofthe CRISPR complex (comprising the guide sequence hybridized to thetarget sequence and complexed with one or more Cas proteins) results incleavage of one or both strands in or near (e.g. within 1, 2, 3, 4, 5,6, 7, 8, 9, 10, 20, 50, or more base pairs from) the target sequence.Without wishing to be bound by theory, the tracr sequence, which maycomprise or consist of all or a portion of a wildtype tracr sequence(e.g. about or more than about 20, 26, 32, 45, 48, 54, 63, 67, 85, ormore nucleotides of a wild-type tracr sequence), may also form part ofthe CRISPR complex, such as by hybridization along at least a portion ofthe tracr sequence to all or a portion of a tracr mate sequence that isoperably linked to the guide sequence. In some embodiments, the tracrsequence has sufficient complementarity to a tracr mate sequence tohybridize and participate in formation of the CRISPR complex.

As with the target sequence, in some embodiments, completecomplementarity is not necessarily needed. In some embodiments, thetracr sequence has at least 50%, 60%, 70%, 80%, 90%, 95% or 99% ofsequence complementarity along the length of the tracr mate sequencewhen optimally aligned. In some embodiments, one or more vectors drivingexpression of one or more elements of the CRISPR system are introducedinto the cell such that expression of the elements of the CRISPR systemdirect formation of the CRISPR complex at one or more target sites. Forexample, a Cas enzyme, a guide sequence linked to a tracr-mate sequence,and a tracr sequence could each be operably linked to separateregulatory elements on separate vectors. Alternatively, two or more ofthe elements expressed from the same or different regulatory elements,may be combined in a single vector, with one or more additional vectorsproviding any components of the CRISPR system not included in the firstvector. In some embodiments, CRISPR system elements that are combined ina single vector may be arranged in any suitable orientation, such as oneelement located 5′ with respect to (“upstream” of) or 3′ with respect to(“downstream” of) a second element. The coding sequence of one elementmay be located on the same or opposite strand of the coding sequence ofa second element, and oriented in the same or opposite direction. Insome embodiments, a single promoter drives expression of a transcriptencoding a CRISPR enzyme and one or more of the guide sequence, tracrmate sequence (optionally operably linked to the guide sequence), and atracr sequence embedded within one or more intron sequences (e.g. eachin a different intron, two or more in at least one intron, or all in asingle intron). In some embodiments, the CRISPR enzyme, guide sequence,tracr mate sequence, and tracr sequence are operably linked to andexpressed from the same promoter.

In some embodiments, a vector comprises a regulatory element operablylinked to an enzyme-coding sequence encoding a CRISPR RNA-guidedendonuclease. In some embodiments, a vector comprises a regulatoryelement operably linked to an enzyme-coding sequence encoding the CRISPRenzyme, such as a Cas protein. Non-limiting examples of Cas proteinsinclude Cas1, Cas IB, Cas2, Cas3, Cas4, Cas5, Cas6, Cas7, Cas8, Cas9(also known as Csn1 and Csx12), Cas10, Csy1, Csy2, Csy3, Cse1, Cse2,Csc1, Csc2, Csa5, Csn2, Csm2, Csm3, Csm4, Csm5, Csm6, Cmr1, Cmr3, Cmr4,Cmr5, Cmr6, Csb1, Csb2, Csb3, Csx17, Csx14, Csx10, Csx16, CsaX, Csx3,Csx1, Csxl5, Csf1, Csf2, Csf3, Csf4, Cpf1, homologs thereof, or modifiedversions thereof. (see WO 2015/200334, incorporated herein byreference). These enzymes are known; for example, the amino acidsequence of S. pyogenes Cas9 protein may be found in the SwissProtdatabase under accession number Q99ZW2 (incorporated herein byreference).

Cas proteins generally comprise at least one RNA recognition or bindingdomain. Such domains can interact with guide RNAs (gRNAs, described inmore detail below). Cas proteins can also comprise nuclease domains, forexample endonuclease domains (e.g., DNase or RNase domains), DNA bindingdomains, helicase domains, protein-protein interaction domains,dimerization domains, and other domains. A nuclease domain possessescatalytic activity for nucleic acid cleavage. Cleavage includes thebreakage of the covalent bonds of a nucleic acid molecule. Cleavage canproduce blunt ends or staggered ends, and it can be single-stranded ordouble-stranded.

Examples of Cas proteins include Cas1, Cas IB, Cas2, Cas3, Cas4, Cas5,Cas5e (CasD), Cas6, Cas6e, Cas6f, Cas7, Cas8a1, Cas8a2, Cas8b, Cas8c,Cas9 (Csn1 or Csx12), Cas10, Cas10d, CasF, CasG, CasH, Csy1, Csy2, Csy3,Cse1 (CasA), Cse2 (CasB), Cse3 (CasE), Cse4 (CasC), Csc1, Csc2, Csa5,Csn2, Csm2, Csm3, Csm4, Csm5, Csm6, Cmr1, Cmr3, Cmr4, Cmr5, Cmr6, Csb1,Csb2, Csb3, Csx17, Csx14, Csx1O, Csx16, CsaX, Csx3, Csx1, Csx15, Csf1,Csf2, Csf3, Csf4, and Cul966, and homologs or modified versions thereof(see WO 2015/200334, incorporated herein by reference).

Any Cas protein that induces a nick or double-strand break into adesired recognition site can be used in the methods and compositionsdisclosed herein.

In general, a guide sequence is any polynucleotide sequence havingsufficient complementarity with a target polynucleotide sequence tohybridize with the target sequence and direct sequence-specific bindingof the CRISPR complex to the target sequence. In some embodiments, thedegree of complementarity between a guide sequence and its correspondingtarget sequence, when optimally aligned using a suitable alignmentalgorithm, is about or more than about 50%, 60%, 75%, 80%, 85%, 90%,95%, 97.5%, 99%, or more.

Optimal alignment may be determined with the use of any suitablealgorithm for aligning sequences, non-limiting example of which includethe Smith-Waterman algorithm, the Needleman-Wunsch algorithm, algorithmsbased on the Burrows-Wheeler Transform (e.g. the Burrows WheelerAligner), ClustalW, Clustal X, BLAT, Novoalign (Novocraft Technologies,ELAND (Illumina, San Diego, Calif.), SOAP (available atsoap.genomics.org.cn), and Maq (available at maq.sourceforge.net). Insome embodiments, a guide sequence is about or more than about 5, 10,11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28,29, 30, 35, 40, 45, 50, 75, or more nucleotides in length. In someembodiments, a guide sequence is less than about 75, 50, 45, 40, 35, 30,25, 20, 15, 12, or fewer nucleotides in length. The ability of a guidesequence to direct sequence-specific binding of the CRISPR complex to atarget sequence may be assessed by any suitable assay. For example, thecomponents of the CRISPR system sufficient to form the CRISPR complex,including the guide sequence to be tested, may be provided to the cellhaving the corresponding target sequence, such as by transfection withvectors encoding the components of the CRISPR sequence, followed by anassessment of preferential cleavage within the target sequence, such asby Surveyor assay as described herein. Similarly, cleavage of a targetpolynucleotide sequence may be evaluated in a test tube by providing thetarget sequence, components of the CRISPR complex, including the guidesequence to be tested and a control guide sequence different from thetest guide sequence, and comparing binding or rate of cleavage at thetarget sequence between the test and control guide sequence reactions.

A guide sequence may be selected to target any target sequence. In someembodiments, the target sequence is a sequence within a genome of acell, and in particular, a protein of interest targeted for controlleddegradation through the engineering of an endogenous protein-dTAGhybrid. Exemplary target sequences include those that are unique in thetarget genome which provide for insertion of the dTAG donor nucleic acidin an in-frame orientation. In some embodiments, a guide sequence isselected to reduce the degree of secondary structure within the guidesequence. Secondary structure may be determined by any suitablepolynucleotide folding algorithm.

In general, a tracr mate sequence includes any sequence that hassufficient complementarity with a tracr sequence to promote one or moreof: (1) excision of a guide sequence flanked by tracr mate sequences ina cell containing the corresponding tracr sequence; and (2) formation ofa CRISPR complex at a target sequence, wherein the CRISPR complexcomprises the tracr mate sequence hybridized to the tracr sequence. Ingeneral, degree of complementarity is with reference to the optimalalignment of the tracr mate sequence and tracr sequence, along thelength of the shorter of the two sequences.

As contemplated herein, the CRISPR-Cas system is used to insert anucleic acid sequence encoding a dTAG in-frame with the genomic sequenceencoding a protein of interest in a eukaryotic, for example, human cell.In some embodiments, the method comprises allowing the CRISPR complex tobind to the genomic sequence of the targeted protein of interest toeffect cleavage of the genomic sequence, wherein the CRISPR complexcomprises the CRISPR enzyme complexed with a guide sequence hybridizedto a target sequence within said target polynucleotide, wherein saidguide sequence is linked to a tracr mate sequence which in turnhybridizes to a tracr sequence.

In some aspects, the methods include modifying expression of apolynucleotide in a eukaryotic cell by introducing a nucleic acidencoding a dTAG.

In some aspects, the polypeptides of the CRISPR-Cas system and donorsequence are administered or introduced to the cell. The nucleic acidstypically are administered in the form of an expression vector, such asa viral expression vector. In some aspects, the expression vector is aretroviral expression vector, an adenoviral expression vector, a DNAplasmid expression vector, or an AAV expression vector. In some aspects,one or more polynucleotides encoding CRISPR-Cas system and donorsequence delivered to the cell. In some aspects, the delivery is bydelivery of more than one vectors.

Methods of delivering nucleic acid sequences to cells as describedherein are described, for example, in U.S. Pat. Nos. 8,586,526;6,453,242; 6,503,717; 6,534,261; 6,599,692; 6,607,882; 6,689,558;6,824,978; 6,933,113; 6,979,539; 7,013,219; and 7,163,824, thedisclosures of all of which are incorporated by reference herein intheir entireties.

The various polynucleotides as described herein may also be deliveredusing vectors containing sequences encoding one or more of compositionsdescribed herein. Any vector systems may be used including, but notlimited to, plasmid vectors, retroviral vectors, lentiviral vectors,adenovirus vectors, poxvirus vectors; herpesvirus vectors andadeno-associated virus vectors, etc. See, also, U.S. Pat. Nos.6,534,261; 6,607,882; 6,824,978; 6,933,113; 6,979,539; 7,013,219; and7,163,824, incorporated by reference herein in their entireties.

Methods of non-viral delivery of nucleic acids include lipofection,nucleofection, microinjection, biolistics, virosomes, liposomes,immunoliposomes, polycation or lipid:nucleic acid conjugates, naked DNA,artificial virions, and agent-enhanced uptake of DNA. Lipofection isdescribed in e.g., U.S. Pat. Nos. 5,049,386, 4,946,787; and 4,897,355)and lipofection reagents are sold commercially (e.g., Transfectam™ andLipofectin™). Cationic and neutral lipids that are suitable forefficient receptor-recognition lipofection of polynucleotides includethose of Feigner, WO 1991/17424 and WO 1991/16024. Delivery can be tocells (e.g. in vitro or ex vivo administration) or target tissues (e.g.in vivo administration).

In some embodiments, delivery is via the use of RNA or DNA viral basedsystems for the delivery of nucleic acids. Viral vectors in some aspectsmay be administered directly to patients (in vivo) or they can be usedto treat cells in vitro or ex vivo, and then administered to patients.Viral-based systems in some embodiments include retroviral, lentivirus,adenoviral, adeno-associated and herpes simplex virus vectors for genetransfer. The tropism of a retrovirus can be altered by incorporatingforeign envelope proteins, expanding the potential target population oftarget cells. Lentiviral vectors are retroviral vectors that are able totransduce or infect non-dividing cells and typically produce high viraltiters. Selection of a retroviral gene transfer system depends on thetarget tissue. Retroviral vectors are comprised of cis-acting longterminal repeats with packaging capacity for up to 6-10 kb of foreignsequence. The minimum cis-acting LTRs are sufficient for replication andpackaging of the vectors, which are then used to integrate thetherapeutic gene into the target cell to provide permanent transgeneexpression. Widely used retroviral vectors include those based uponmurine leukemia virus (MuLV), gibbon ape leukemia virus (GaLV), SimianImmunodeficiency virus (SIV), human immunodeficiency virus (HIV), andcombinations thereof (see, e.g., Buchscher et al., J. Virol. 66,(1992):2731-2739; Johann et al., J. Virol. 66, (1992):1635-1640;Sommerfelt et al., J. Virol. 176, (1990):58-69; Wilson et al., J. Virol.63, (1989):2374-2378; Miller et al., J. Virol. 65, (1991):2220-2224; andPCT/US94/05700).

In applications in which transient expression is preferred, adenoviralbased systems can be used. Adenoviral based vectors are capable of veryhigh transduction efficiency in many cell types and do not require celldivision. With such vectors, high titer and high levels of expressionhave been obtained. This vector can be produced in large quantities in arelatively simple system. Adeno-associated virus (“AAV”) vectors arealso used to transduce cells with target nucleic acids, e.g., in the invitro production of nucleic acids and peptides, and for in vivo and exvivo gene therapy procedures (see, e.g., West et al., Virology 160,(1987):38-47; U.S. Pat. No. 4,797,368; WO 1993/24641; Kotin, Human GeneTherapy 5, (1994):793-801; Muzyczka, J. Clin. Invest. 94, (1994): 1351.Construction of recombinant AAV vectors is described in a number ofpublications, including U.S. Pat. No. 5,173,414; Tratschin et al., Mol.Cell. Biol. 5, (1985):3251-3260; Tratschin, et al., Mol. Cell. Biol. 4,(1984):2072-2081; Hermonat & Muzyczka, PNAS 81, (1984):6466-6470; andSamulski et al., J. Virol. 63, (1989):3822-3828.

At least six viral vector approaches are currently available for genetransfer in clinical trials, which utilize approaches that involvecomplementation of defective vectors by genes inserted into helper celllines to generate the transducing agent.

pLASN and MFG-S are examples of retroviral vectors that have been usedin clinical trials (Dunbar et al., Blood 85, (1995):3048-305; Kohn etal., Nat. Med. 1, (1995):1017-1023; Malech et al., PNAS 94(22),(1997):12133-12138). PA317/pLASN was the first therapeutic vector usedin a gene therapy trial. (Blaese et al., Science 270, (1995):475-480).Transduction efficiencies of 50% or greater have been observed for MFG-Spackaged vectors. (Ellem et al., Immunol Immunother. 44(1),(1997):10-20; and Dranoff et al., Hum. Gene Ther. 1, (1997):111-112).

Vectors suitable for introduction of polynucleotides described hereinalso include non-integrating lentivirus vectors (IDLV). See, forexample, Naldini et al. Proc. Natl. Acad. Sci. 93, (1996):11382-11388;Dull et al. J Virol. 72, (1998):8463-8471; Zuffery et al. J. Virol. 72,(1998):9873-9880; Follenzi et al. Nature Genetics 25, (2000):217-222;and U.S. 2009/0117617.

Recombinant adeno-associated virus vectors (rAAV) may also be used todeliver the compositions described herein. All vectors are derived froma plasmid that retains only the AAV inverted terminal repeats flankingthe transgene expression cassette. Efficient gene transfer and stabletransgene delivery are key features for this vector system. (Wagner etal., Lancet 351, (1998):9117 1702-3, and Kearns et al., Gene Ther. 9,(1996):748-55). Other AAV serotypes, including AAV1, AAV3, AAV4, AAV5,AAV6, AAV8, AAV9 and AAVrh10, pseudotyped AAV such as AAV2/8, AAV2/5 andAAV2/6 and all variants thereof, can also be used in accordance with thepresent invention.

Replication-deficient recombinant adenoviral vectors (Ad) can beproduced at high titer and readily infect a number of different celltypes. Most adenovirus vectors are engineered such that a transgenereplaces the Ad E1a, E1b, and/or E3 genes; subsequently the replicationdefective vector is propagated in human 293 cells that supply deletedgene function in trans. Ad vectors can transduce multiple types oftissues in vivo, including non-dividing, differentiated cells such asthose found in liver, kidney and muscle. Conventional Ad vectors have alarge carrying capacity. An example of the use of an Ad vector in aclinical trial involved polynucleotide therapy for antitumorimmunization with intramuscular injection (Sterman et al., Hum. GeneTher. 7, (1998):1083-1089). Additional examples of the use of adenovirusvectors for gene transfer in clinical trials include Rosenecker et al.,Infection 24(1), (1996):5-10; Sterman et al., Hum. Gene Ther. 9(7),(1998):1083-1089; Welsh et al., Hum. Gene Ther. 2, (1995):205-218;Alvarez et al., Hum. Gene Ther. 5, (1997):597-613; Topf et al., GeneTher. 5, (1998):507-513; Sterman et al., Hum. Gene Ther. 7,(1998):1083-1089.

Packaging cells are used to form virus particles that are capable ofinfecting a host cell. Such cells include 293 cells, which packageadenovirus, and ψ² cells or PA317 cells, which package retrovirus. Viralvectors used in gene therapy are usually generated by a producer cellline that packages a nucleic acid vector into a viral particle. Thevectors typically contain the minimal viral sequences required forpackaging and subsequent integration into a host (if applicable), otherviral sequences being replaced by an expression cassette encoding theprotein to be expressed. The missing viral functions are supplied intrans by the packaging cell line. For example, AAV vectors used in genetherapy typically only possess inverted terminal repeat (ITR) sequencesfrom the AAV genome which are required for packaging and integrationinto the host genome. Viral DNA is packaged in a cell line, whichcontains a helper plasmid encoding the other AAV genes, namely rep andcap, but lacking ITR sequences. The cell line is also infected withadenovirus as a helper. The helper virus promotes replication of the AAVvector and expression of AAV genes from the helper plasmid. The helperplasmid is not packaged in significant amounts due to a lack of ITRsequences. Contamination with adenovirus can be reduced by, e.g., heattreatment to which adenovirus is more sensitive than AAV.

The vector can be delivered with a high degree of specificity to aparticular tissue type. Accordingly, a viral vector can be modified tohave specificity for a given cell type by expressing a ligand as afusion protein with a viral coat protein on the outer surface of thevirus. The ligand is chosen to have affinity for a receptor known to bepresent on the cell type of interest. For example, Han et al., Proc.Natl. Acad. Sci. 92, (1995):9747-9751, reported that Moloney murineleukemia virus can be modified to express human heregulin fused to gp70,and the recombinant virus infects certain human breast cancer cellsexpressing human epidermal growth factor receptor. This principle can beextended to other virus-target cell pairs, in which the target cellexpresses a receptor and the virus expresses a fusion protein comprisinga ligand for the cell-surface receptor. For example, filamentous phagecan be engineered to display antibody fragments (e.g., FAB or Fv) havingspecific binding affinity for virtually any chosen cellular receptor.Although the above description applies primarily to viral vectors, thesame principles can be applied to nonviral vectors. Such vectors can beengineered to contain specific uptake sequences which favor uptake byspecific target cells.

Vectors can be delivered in vivo by administration to an individualsubject, typically by systemic administration (e.g., intravenous,intraperitoneal, intramuscular, intrathecal, intratracheal, subdermal,or intracranial infusion) or topical application, as described below.Alternatively, vectors can be delivered to cells ex vivo, such as cellsexplanted from an individual patient (e.g., lymphocytes, bone marrowaspirates, tissue biopsy) or universal donor hematopoietic stem cells,followed by reimplantation of the cells into a patient, usually afterselection for cells which have incorporated the vector.

Vectors (e.g., retroviruses, adenoviruses, liposomes, etc.) containingnucleases and/or donor constructs can also be administered directly toan organism for transduction of cells in vivo. Alternatively, naked DNAcan be administered. Administration is by any of the routes normallyused for introducing a molecule into ultimate contact with blood ortissue cells including, but not limited to, injection, infusion, topicalapplication and electroporation. Suitable methods of administering suchnucleic acids are available and well known to those of skill in the art,and, although more than one route can be used to administer a particularcomposition, a particular route can often provide a more immediate andmore effective reaction than another route.

In some embodiments, the polypeptides of the CRISPR-Cas system aresynthesized in situ in the cell as a result of the introduction ofpolynucleotides encoding the polypeptides into the cell. In someaspects, the polypeptides of the CRISP-Cas system could be producedoutside the cell and then introduced thereto. Methods for introducing aCRISPR-Cas polynucleotide construct into animal cells are known andinclude, as non-limiting examples stable transformation methods whereinthe polynucleotide construct is integrated into the genome of the cell,transient transformation methods wherein the polynucleotide construct isnot integrated into the genome of the cell, and virus mediated methods,as described herein. Preferably, the CRISPR-Cas polynucleotide istransiently expressed and not integrated into the genome of the cell. Insome embodiments, the CRISPR-Cas polynucleotides may be introduced intothe cell by for example, recombinant viral vectors (e.g. retroviruses,adenoviruses), liposome and the like. For example, in some aspects,transient transformation methods include microinjection,electroporation, or particle bombardment. In some embodiments, theCRISPR-Cas polynucleotides may be included in vectors, more particularlyplasmids or virus, in view of being expressed in the cells.

In some embodiments, non-CRISPR-CAS viral and non-viral based genetransfer methods can be used to insert nucleic acids encoding a dTAG inframe in the genomic locus of a protein of interest in mammalian cellsor target tissues. Such methods can be used to administer nucleic acidsencoding components of a ZFP, ZFN, TALE, and/or TALEN system to cells inculture, or in a host organism including a donor sequence encoding adTAG for in-frame insertion into the genomic locus of a protein ofinterest.

Non-viral vector delivery systems include DNA plasmids, RNA (e.g. atranscript of a vector described herein), naked nucleic acid, andnucleic acid complexed with a delivery vehicle, such as a liposome.Viral vector delivery systems include DNA and RNA viruses, which haveeither episomal or integrated genomes after delivery to the cell. For areview of gene therapy procedures, see Anderson, Science 256,(1992):808-813; Nabel & Feigner, TIBTECH 11, (1993):211-217; Mitani &Caskey, TIBTECH 11, (1993): 162-166; Dillon. TIBTECH 11, (1993):167-173; Miller, Nature 357, (1992):455-460; Van Brunt, Biotechnology6(10), (1988): 1149-1154; Vigne, Restorative Neurology and Neuroscience8, (1995):35-36; Kremer & Perricaudet, British Medical Bulletin 51(1),(1995):31-44; and Yu et al., Gene Therapy 1, (1994): 13-26.

The preparation of lipid:nucleic acid complexes, including targetedliposomes such as immunolipid complexes, is well known to one of skillin the art (see, e.g., Crystal, Science 270, (1995):404-410; Blaese etal., Cancer Gene Ther. 2, (1995):291-297; Behr et al., BioconjugateChem. 5, (1994):382-389; Remy et al., Bioconjugate Chem. 5,(1994):647-654; Gao et al., Gene Therapy 2, (1995):710-722; Ahmad etal., Cancer Res. 52, (1992):4817-4820; and U.S. Pat. Nos. 4,186,183,4,217,344, 4,235,871, 4,261,975, 4,485,054, 4,501,728, 4,774,085,4,837,028, and 4,946,787).

Additional methods of delivery include the use of packaging the nucleicacids to be delivered into EnGeneIC delivery vehicles (EDVs). These EDVsare specifically delivered to target tissues using bispecific antibodieswhere one arm of the antibody has specificity for the target tissue andthe other has specificity for the EDV. The antibody brings the EDVs tothe target cell surface and then the EDV is brought into the cell byendocytosis. Once in the cell, the contents are released (see MacDiarmidet al Nature Biotechnology 27(7), (2009):643).

D. Heterobifunctional Compounds

The present application includes the use of a heterobifunctionalcompound which has (i) a moiety that binds to a ubiquitin ligase and(ii) a targeting moiety which binds to a dTAG which has been fused to anendogenous protein intended for ubiquitination and proteasomaldegradation. In one embodiment the heterobifunctional compound binds toa dTAG that is mutated to have selectivity over the correspondingendogenous protein (i.e. the dTAG Targeting Ligand binds dTAG but doesnot significantly bind to the naturally occurring (and in someembodiments, will not significantly bind to a mutant or variant proteinexpressed by the host)).

Strategies harnessing the ubiquitin proteasome pathway (UPP) toselectively target and degrade proteins have been employed forpost-translational control of protein function. Heterobifunctionalcompounds, are composed of a target protein-binding ligand and an E3ubiquitin ligase ligand. Heterobifunctional compounds, are capable ofinduced proteasome-mediated degradation of selected proteins via theirrecruitment to E3 ubiquitin ligase and subsequent ubiquitination. Thesedrug-like molecules offer the possibility of reversible,dose-responsive, tunable, temporal control over protein levels. An earlydescription of such compounds was provided in U.S. Pat. No. 7,041,298,titled “Proteolysis Targeting Chimeric Pharmaceutical,” filed inSeptember 2000 by Deshales et al. and granted in May 2006. Thepublication by Sakamoto et al. (PNAS 98(15) (2001): 8554-8559), titled“PROTACS: Chimeric Molecules that Target Proteins to the Skp1-Cullin FBox Complex for Ubiquitination and Degradation,” describes aheterobifunctional compound consisting of a small molecule binder ofMAP-AP-2 linked to a peptide capable of binding the F-box proteinβ-TRCP, the disclosure of which is also provided in U.S. Pat. No.7,041,298. The publication by Sakamoto et al. (Molecular and CellularProteomics 2 (2003):1350-1358), titled “Development of PROTACS to TargetCancer-promoting Proteins for Ubiquitination and Degradation,” describesan analogous heterobifunctional compound (PROTAC2) that instead ofdegrading MAP-AP-2 degrades estrogen and androgen receptors. Thepublication by Schneekloth et al. (JACS 126 (2004):3748-3754), titled“Chemical Genetic Control of Protein Levels: Selective in vivo TargetedDegradation,” describes an analogous heterobifunctional compound(PROTAC3) that targets the FK506 binding protein (FKBP12) and shows bothPROTAC2 and PROTAC3 hit their respective targets with green fluorescentprotein (GFP) imaging. The publication by Schneekloth et al.(ChemBioChem 6 (2005)40-46) titled “Chemical Approaches to ControllingIntracellular Protein Degradation” described the state of the field atthe time, using the technology. The publication by Schneekloth et al.(BMCL 18(22) (2008):5904-5908), titled “Targeted Intracellular ProteinDegradation Induced by a Small Molecule: En Route to ChemicalProteomics,” describes a heterobifunctional compound that consist of twosmall molecules linked by PEG that in vivo degrades the androgenreceptor by concurrently binding the androgen receptor and Ubiquitin E3ligase. WO 2013/170147 to Crews et al., titled “Compounds Useful forPromoting Protein Degradation and Methods Using Same,” describescompounds comprising a protein degradation moiety covalently bound to alinker, wherein the ClogP of the compound is equal to or higher than1.5. A review of the foregoing publications by Buckley et al. (Angew.Chem. Int. Ed. 53 (2014):2312-2330) is titled “Small-Molecule Control ofIntraceullular Protein Levels through Modulation of the UbiquitinProteasome System.” WO 2015/160845 assigned to Arvinas Inc., titled“Imide Based Modulators of Proteolysis and Associated methods of Use,”describes the use of Degron technology with thalidomide to utilizecereblon as the E3 ligase protein. The following publication by J. Lu etal. (Chemistry and Biol. 22(6) (2015):755-763), titled “Hijacking the E3Ubiquitin Ligase Cereblon to efficiently Target BDR4,” similarlydescribes thalidomide based compounds useful for degrading BDR4.Additional publications describing this technology include Bondeson etal. (Nature Chemical Biology 11 (2015):611-617), Gustafson et al.(Angew. Chem. Int. Ed. 54 (2015):9659-9662), Buckley et al. (ACS Chem.Bio. 10 (2015):1831-1837), U.S. 2016/0058872 assigned to Arvinas Inc.titled “Imide Based Modulators of Proteolysis and Associated Methods ofUse”, U.S. 2016/0045607 assigned to Arvinas Inc. titled“Estrogen-related Receptor Alpha Based PROTAC Compounds and AssociatedMethods of Use”, U.S. 2014/0356322 assigned to Yale University,GlaxoSmithKline, and Cambridge Enterprise Limited University ofCambridge titled “Compounds and Methods for the Enhanced Degradation ofTargeted Proteins & Other Polypeptides by an E3 Ubiquitin Ligase”, Laiet al. (Angew. Chem. Int. Ed. 55 (2016):807-810), Toure et al. (Angew.Chem. Int. Ed. 55 (2016):1966-1973), and US 2016/0176916 assigned toDana Farber Cancer Institute titled “Methods to Induce Targeted ProteinDegradation Through Bifunctional Molecules.”

Other descriptions of targeted protein degradation technology includeItoh et al. (JACS 132(16) (2010):5820-5826), titled “Protein KnockdownUsing Methyl Bestatin-Ligand Hybrid Molecules: Design and Synthesis ofInducers of Ubiquitination-Mediated Degradation of Cellular RetinoicAcid-Binding Proteins,” which describes a small molecule linked to apeptide that utilizes E3 ubiquitin ligase to degraded retinoicacid-binding proteins, and Winter et al. (Science 348 (2015):1376-1381),titled “Phthalimide Conjugation as a Strategy for in vivo Target ProteinDegradation,” describes thalidomide based targeted protein degradationtechnology.

Heterobifunctional compounds useful for present invention may be anyheterobifunctional compound capable of binding to a dTAG to inducedegradation. Heterobifunctional compounds are generally known in theart, for example, see U.S. Pat. No. 7,041,298; Sakamoto et al. (PNAS,2001, 98(15): 8554-8559); Sakamoto et al. (Molecular and CellularProteomics 2 (2003)1350-1358); Schneekloth et al. (JACS 126(2004):3748-3754); Schneekloth et al. (ChemBioChem 6 (2005):40-46);Schneekloth et al. (BMCL 18(22) (2008):5904-5908); WO 2013/170147;Buckley et al. (Angew. Chem. Int. Ed. 53 (2014):2312-2330); WO2015/160845; Lu et al. (Chemistry and Biol. 22(6) (2015):755-763);Bondeson et al. (Nature Chemical Biology 11 (2015):611-617); Gustafsonet al. (Angew. Chem. Int. Ed. 54 (2015):9659-9662); Buckley et al. (ACSChem. Bio. 10 (2015):1831-1837); U.S. 2016/0058872 assigned to ArvinasInc. titled “Imide Based Modulators of Proteolysis and AssociatedMethods of Use”, U.S. 2016/0045607 assigned to Arvinas Inc. titled“Estrogen-related Receptor Alpha Based PROTAC Compounds and AssociatedMethods of Use”, U.S. 2014/0356322 assigned to Yale University,GlaxoSmithKline, and Cambridge Enterprise Limited University ofCambridge titled “Compounds and Methods for the Enhanced Degradation ofTargeted Proteins & Other Polypeptides by an E3 Ubiquitin Ligase”, U.S.2016/0176916 assigned to Dana-Farber Cancer Institute, Inc. titled“Methods to Induce Targeted Protein Degradation Through BifunctionalMolecules”, Lai et al. (Angew. Chem. Int. Ed. 55 (2016):807-810); Toureet al. (Angew. Chem. Int. Ed. 55 (2016):1966-1973); Itoh et al. (JACS132(16) (2010):5820-5826); and Winter et al. (Science 348(2015):1376-1381), each of which is incorporated herein by reference.

In general, heterobifunctional compounds suitable for use in the presentapplication have the general structure:

Degron-Linker-dTAG Targeting Ligand

wherein the Linker is covalently bound to a Degron and a dTAG TargetingLigand, the Degron is a compound capable of binding to a ubiquitinligase such as an E3 Ubiquitin Ligase (e.g., cereblon), and the dTAGTargeting Ligand is capable of binding to the dTAG on the endogenousprotein-dTAG hybrid protein.

In certain embodiments, the present application utilizes a compound ofFormula I or Formula II:

wherein:

the Linker is a group that covalently binds to the dTAG Targeting Ligandand Y; and

the dTAG Targeting Ligand is capable of binding to a dTAG target orbeing bound by a dTAG target that allows tagging to occur.

In certain embodiments, the present application provides a compound ofFormula (I), or an enantiomer, diastereomer, stereoisomer, orpharmaceutically acceptable salt thereof,

wherein:

the Linker (L) is a group that covalently binds to the dTAG TargetingLigand and Y; and

the dTAG Targeting Ligand is capable of binding to or binds to a dTAG;

and wherein X1, X2, Y, R₁, R₂, R₂′, R₃, R₃′, R₄, R₅, m and n are each asdefined herein.

In certain embodiments, the present application provides a compound ofFormula (II), or an enantiomer, diastereomer, stereoisomer, orpharmaceutically acceptable salt thereof,

wherein:

the Linker is a group that covalently binds to the dTAG Targeting Ligandand Y; and

the dTAG Targeting Ligand is capable of binding to or binds to a dTAG;

and wherein X₁, X₂, Y, R₁, R₂, R₂′, R₃, R₃′, R₄, R₅, m and n are each asdefined herein.

In certain embodiments, the present invention uses a compound of FormulaIII, Formula IV, Formula V, Formula VI, Formula VII, Formula VIII, andFormula IX:

wherein:

the Linker (L) is a group that covalently binds to the dTAG TargetingLigand and Z₂;

the dTAG Targeting Ligand is capable of binding to a target dTAG orbeing bound by a target dTAG;

Z₂ is a bond, alkyl, —O, —C(O)NR₂, —NR⁶C(O), —NH, or —NR⁶;

R⁶ is H, alkyl, —C(O)alkyl, or —C(O)H;

X₃ is independently selected from O, S, and CH₂;

W₂ is independently selected from the group CH₂, CHR, C═O, SO₂, NH, andN-alkyl;

Y₂ is independently selected from the group NH, N-alkyl, N-aryl,N-hetaryl, N-cycloalkyl, N-heterocyclyl, O, and S;

G and G′ are independently selected from the group H, alkyl, OH,CH₂-heterocyclyl optionally substituted with R′, and benzyl optionallysubstituted with R′;

Q₁, Q₂, Q₃, and Q₄ are independently selected from CH, N, CR′, andN-oxide.

A₂ is independently selected from the group alkyl, cycloalkyl, Cl and F;

R⁷ is selected from: CONR′R″, OR′, NR′R″, SR′, SO₂R′, SO₂NR′R″, CR′R″—,—CR′NR′R″—, -aryl, -hetaryl, -alkyl, -cycloalkyl, -heterocyclyl,—P(O)(OR′)R″, P(O)R′R″, —OP(O)(OR′)R″, —OP(O)R′R″, —Cl, —F, —Br, —I,—CF₃, —CN, NR′SO₂NR′R″, —NR′CONR′R″, —CONR′COR″, —NR′C(═N—CN)NR′R″,—C(═N—CN)NR′R″, —NR′C(═N—CN)R″, —NR′C(═C—NO₂)NR′R″, —SO₂NR′COR″, —NO₂,CO₂R′, —C(C═N—OR′)R″, —CR′═CR′R″, —CCR′, —S(C═O)(C═N—R′)R″, —SF₅ and—OCF₃

R′ and R″ are independently selected from a bond, H, alkyl, cycloalkyl,aryl, heteroaryl, heterocyclyl

Non-limiting examples of dTAG Targeting Ligands for use in the presentinvention include:

In some embodiments the dTAG Targeting Ligand targets a mutatedendogenous target or a non-endogenous target.

Degron

The Degron is a compound moiety that links a dTAG, through the Linkerand dTAG Targeting Ligand, to a ubiquitin ligase for proteosomaldegradation. In certain embodiments, the Degron is a compound that iscapable of binding to or binds to a ubiquitin ligase. In furtherembodiments, the Degron is a compound that is capable of binding to orbinds to a E3 Ubiquitin Ligase. In further embodiments, the Degron is acompound that is capable of binding to or binds to cereblon. In furtherembodiments, the Degron is a thalidomide or a derivative or analogthereof.

In certain embodiments, the Degron is a moiety of Formula D, Formula DO,or Formula D′:

or an enantiomer, diastereomer, or stereoisomer thereof, wherein:

Y is a bond, (CH₂)₁₋₆, (CH₂)₀₋₆—O, (CH₂)₀₋₆—C(O)NR₂′, (CH₂)₀₋₆—NR₂′C(O),(CH₂)₀₋₆—NH, or (CH₂)₀₋₆—NR₂;

X is C(O) or C(R₃)₂;

X₁—X₂ is C(R₃)═N or C(R₃)₂—C(R₃)₂;

each R₁ is independently halogen, OH, C₁-C₆ alkyl, or C₁-C₆ alkoxy;

R₂ is C₁-C₆ alkyl, C(O)—C₁-C₆ alkyl, or C(O)—C₃-C₆ cycloalkyl;

R₂′ is H or C₁-C₆ alkyl;

each R₃ is independently H or C₁-C₃ alkyl;

each R₃′ is independently C₁-C₃ alkyl;

each R₄ is independently H or C₁-C₃ alkyl; or two R₄, together with thecarbon atom to which they are attached, form C(O), a C₃-C₆ carbocycle,or a 4-, 5-, or 6-membered heterocycle comprising 1 or 2 heteroatomsselected from N and O;

R₅ is H, deuterium, C₁-C₃ alkyl, F, or C;

m is 0, 1, 2 or 3; and

n is 0, 1 or 2;

wherein the compound is covalently bonded to another moiety (e.g., acompound, or a Linker) via

In certain embodiments, the Degron is a moiety of Formula D, wherein

In certain embodiments, the Degron is a moiety of Formula D, wherein

In certain embodiments, the Degron is a moiety of Formula D, wherein Xis C(O).

In certain embodiments, the Degron is a moiety of Formula D, wherein Xis C(R₃)₂; and each R₃ is H. In certain embodiments, X is C(R₃)₂; andone of R₃ is H, and the other is C₁-C₃ alkyl selected from methyl,ethyl, and propyl. In certain embodiments, X is C(R₃)₂; and each R₃ isindependently selected from methyl, ethyl, and propyl.

In certain embodiments, the Degron is a moiety of Formula D, whereinX₁—X₂ is C(R₃)═N.

In certain embodiments, X₁—X₂ is CH═N. In certain embodiments, X₁—X₂ isC(R₃)═N; and R₃ is C₁-C₃ alkyl selected from methyl, ethyl, and propyl.In certain embodiments, X₁—X₂ is C(CH₃)═N.

In certain embodiments, the Degron is a moiety of Formula D, whereinX₁—X₂ is C(R₃)₂—C(R₃)₂; and each R₃ is H. In certain embodiments, X₁—X₂is C(R₃)₂—C(R₃)₂; and one of R₃ is H, and the other three R₃ areindependently C₁-C₃ alkyl selected from methyl, ethyl, and propyl. Incertain embodiments, X₁—X₂ is C(R₃)₂—C(R₃)₂; and two of the R₃ are H,and the other two R₃ are independently C₁-C₃ alkyl selected from methyl,ethyl, and propyl. In certain embodiments, X₁—X₂ is C(R₃)₂—C(R₃)₂; andthree of the R₃ are H, and the remaining R₃ is C₁-C₃ alkyl selected frommethyl, ethyl, and propyl.

In certain embodiments, the Degron is a moiety of Formula D, wherein Yis a bond.

In certain embodiments, the Degron is a moiety of Formula D, wherein Yis (CH₂)₁, (CH₂)₂, (CH₂)₃, (CH₂)₄, (CH₂)₅, or (CH₂)₆. In certainembodiments, Y is (CH₂)₁, (CH₂)₂, or (CH₂)₃. In certain embodiments, Yis (CH₂)₁ or (CH₂)₂.

In certain embodiments, the Degron is a moiety of Formula D, wherein Yis O, CH₂—O, (CH₂)₂—O, (CH₂)₃—O, (CH₂)₄—O, (CH₂)₅—O, or (CH₂)₆—O. Incertain embodiments, Y is O, CH₂—O, (CH₂)₂—O, or (CH₂)₃—O. In certainembodiments, Y is O or CH₂—O. In certain embodiments, Y is O.

In certain embodiments, the Degron is a moiety of Formula D, wherein Yis C(O)NR₂′, CH₂—C(O)NR₂′, (CH₂)₂—C(O)NR₂′, (CH₂)₃—C(O)NR₂′,(CH₂)₄—C(O)NR₂′, (CH₂)₅—C(O)NR₂′, or (CH₂)₆—C(O)NR₂′. In certainembodiments, Y is C(O)NR₂′, CH₂—C(O)NR₂′, (CH₂)₂—C(O)NR₂′, or(CH₂)₃—C(O)NR₂′. In certain embodiments, Y is C(O)NR₂′ or CH₂—C(O)NR₂′.In certain embodiments, Y is C(O)NR₂′.

In certain embodiments, the Degron is a moiety of Formula D, wherein Yis NR₂′C(O), CH₂—NR₂′C(O), (CH₂)₂—NR₂′C(O), (CH₂)₃—NR₂′C(O),(CH₂)₄—NR₂′C(O), (CH₂)₅—NR₂′C(O), or (CH₂)₆—NR₂′C(O). In certainembodiments, Y is NR₂′C(O), CH₂—NR₂′C(O), (CH₂)₂—NR₂′C(O), or(CH₂)₃—NR₂′C(O). In certain embodiments, Y is NR₂′C(O) or CH₂—NR₂′C(O).In certain embodiments, Y is NR₂′C(O).

In certain embodiments, the Degron is a moiety of Formula D, wherein R₂′is H. In certain embodiments, the Degron is a moiety of Formula D,wherein R₂′ is selected from methyl, ethyl, propyl, butyl, i-butyl,t-butyl, pentyl, i-pentyl, and hexyl. In certain embodiments, R₂′ isC₁-C₃ alkyl selected from methyl, ethyl, and propyl.

In certain embodiments, the Degron is a moiety of Formula D, wherein Yis NH, CH₂—NH, (CH₂)₂—NH, (CH₂)₃—NH, (CH₂)₄—NH, (CH₂)₅—NH, or (CH₂)₆—NH.In certain embodiments, Y is NH, CH₂—NH, (CH₂)₂—NH, or (CH₂)₃—NH. Incertain embodiments, Y is NH or CH₂—NH. In certain embodiments, Y is NH.

In certain embodiments, the Degron is a moiety of Formula D, wherein Yis NR₂, CH₂—NR₂, (CH₂)₂—NR₂, (CH₂)₃—NR₂, (CH₂)₄—NR₂, (CH₂)₅—NR₂, or(CH₂)₆—NR₂. In certain embodiments, Y is NR₂, CH₂—NR₂, (CH₂)₂—NR₂, or(CH₂)₃—NR₂. In certain embodiments, Y is NR₂ or CH₂—NR₂.

In certain embodiments, Y is NR₂.

In certain embodiments, the Degron is a moiety of Formula D, wherein R₂is selected from methyl, ethyl, propyl, butyl, i-butyl, t-butyl, pentyl,i-pentyl, and hexyl. In certain embodiments, R₂ is C₁-C₃ alkyl selectedfrom methyl, ethyl, and propyl.

In certain embodiments, the Degron is a moiety of Formula D, wherein R₂is selected from C(O)-methyl, C(O)-ethyl, C(O)-propyl, C(O)-butyl,C(O)-i-butyl, C(O)-t-butyl, C(O)-pentyl, C(O)-i-pentyl, and C(O)-hexyl.In certain embodiments, R₂ is C(O)—C₁-C₃ alkyl selected fromC(O)-methyl, C(O)-ethyl, and C(O)-propyl.

In certain embodiments, the Degron is a moiety of Formula D, wherein R₂is selected from C(O)-cyclopropyl, C(O)-cyclobutyl, C(O)-cyclopentyl,and C(O)-cyclohexyl. In certain embodiments, R₂ is C(O)-cyclopropyl.

In certain embodiments, the Degron is a moiety of Formula D, wherein R₃is H.

In certain embodiments, the Degron is a moiety of Formula D, wherein R₃is C₁-C₃ alkyl selected from methyl, ethyl, and propyl. In certainembodiments, R₃ is methyl.

In certain embodiments, the Degron is a moiety of Formula D, wherein nis 0.

In certain embodiments, the Degron is a moiety of Formula D, wherein nis 1.

In certain embodiments, the Degron is a moiety of Formula D, wherein nis 2.

In certain embodiments, the Degron is a moiety of Formula D, whereineach R₃′ is independently C₁-C₃ alkyl selected from methyl, ethyl, andpropyl.

In certain embodiments, the Degron is a moiety of Formula D, wherein mis 0.

In certain embodiments, the Degron is a moiety of Formula D, wherein mis 1.

In certain embodiments, the Degron is a moiety of Formula D, wherein mis 2.

In certain embodiments, the Degron is a moiety of Formula D, wherein mis 3.

In certain embodiments, the Degron is a moiety of Formula D, whereineach R₁ is independently selected from halogen (e.g., F, Cl, Br, and I),OH, C₁-C₆ alkyl (e.g., methyl, ethyl, propyl, butyl, i-butyl, t-butyl,pentyl, i-pentyl, and hexyl), and C₁-C₆ alkoxy (e.g., methoxy, ethoxy,propoxy, butoxy, i-butoxy, t-butoxy, and pentoxy). In furtherembodiments, the Degron is a moiety of Formula D, wherein each R₁ isindependently selected from F, Cl, OH, methyl, ethyl, propyl, butyl,i-butyl, t-butyl, methoxy, and ethoxy.

In certain embodiments, the Degron is a moiety of Formula D, whereineach R₄ is H.

In certain embodiments, the Degron is a moiety of Formula D, wherein oneof R₄ is H, and the other R₄ is C₁-C₃ alkyl selected from methyl, ethyl,and propyl.

In certain embodiments, the Degron is a moiety of Formula D, whereineach R₄ is independently C₁-C₃ alkyl selected from methyl, ethyl, andpropyl.

In certain embodiments, the Degron is a moiety of Formula D, wherein twoR₄, together with the carbon atom to which they are attached, form C(O).

In certain embodiments, the Degron is a moiety of Formula D, wherein twoR₄, together with the carbon atom to which they are attached, formcyclopropyl, cyclobutyl, cyclopentyl, or cyclohexyl.

In certain embodiments, the Degron is a moiety of Formula D, wherein twoR₄, together with the carbon atom to which they are attached, form a 4-,5-, or 6-membered heterocycle selected from oxetane, azetidine,tetrahydrofuran, pyrrolidine, piperidine, piperazine, and morpholine. Incertain embodiments, two R₄, together with the carbon atom to which theyare attached, form oxetane.

In certain embodiments, the Degron is a moiety of Formula D, wherein R₅is H, deuterium, or C₁-C₃ alkyl. In further embodiments, R₅ is in the(S) or (R) configuration. In further embodiments, R₅ is in the (S)configuration. In certain embodiments, the Degron is a moiety of FormulaD, wherein the compound comprises a racemic mixture of (S)—R₅ and(R)—R₅.

In certain embodiments, the Degron is a moiety of Formula D, wherein R₅is H.

In certain embodiments, the Degron is a moiety of Formula D, wherein R₅is deuterium.

In certain embodiments, the Degron is a moiety of Formula D, wherein R₅is C₁-C₃ alkyl selected from methyl, ethyl, and propyl. In certainembodiments, R₅ is methyl.

In certain embodiments, the Degron is a moiety of Formula D, wherein R₅is F or C₁. In further embodiments, R₅ is in the (S) or (R)configuration. In further embodiments, R₅ is in the (R) configuration.In certain embodiments, the Degron is a moiety of Formula D, wherein thecompound comprises a racemic mixture of (S)—R₅ and (R)—R₅. In certainembodiments, R₅ is F.

In certain embodiments, the Degron is selected from the structures inFIG. 21, wherein X is H, deuterium, C₁-C₃ alkyl, or halogen; and R isthe attachment point for the Linker.

In certain embodiments, the Degron is selected from the structures inFIG. 22.

In certain embodiments, the Degron is selected from the structures inFIG. 23.

Linker

The Linker is a bond or a chemical group that links a dTAG TargetingLigand with a Degron. In certain embodiments the Linker is a carbonchain. In certain embodiments, the carbon chain optionally includes one,two, three, or more heteroatoms selected from N, O, and S. In certainembodiments, the carbon chain comprises only saturated chain carbonatoms. In certain embodiments, the carbon chain optionally comprises twoor more unsaturated chain carbon atoms (e.g., C═C or C≡C). In certainembodiments, one or more chain carbon atoms in the carbon chain areoptionally substituted with one or more substituents (e.g., oxo, C₁-C₆alkyl, C₂-C₆ alkenyl, C₂-C₆ alkynyl, C₁-C₃ alkoxy, OH, halogen, NH₂,NH(C₁-C₃ alkyl), N(C₁-C₃ alkyl)₂, CN, C₃-C₈ cycloalkyl, heterocyclyl,phenyl, and heteroaryl).

In certain embodiments, the Linker includes at least 5 chain atoms(e.g., C, O, N, and S).

In certain embodiments, the Linker comprises less than 20 chain atoms(e.g., C, O, N, and S). In certain embodiments, the Linker comprises 5,6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, or 19 chain atoms (e.g.,C, O, N, and S). In certain embodiments, the Linker comprises 5, 7, 9,11, 13, 15, 17, or 19 chain atoms (e.g., C, O, N, and S). In certainembodiments, the Linker comprises 5, 7, 9, or 11 chain atoms (e.g., C,O, N, and S). In certain embodiments, the Linker comprises 6, 8, 10, 12,14, 16, or 18 chain atoms (e.g., C, O, N, and S). In certainembodiments, the Linker comprises 6, 8, 10, or 12 chain atoms (e.g., C,O, N, and S).

In certain embodiments, the Linker is a carbon chain optionallysubstituted with non-bulky substituents (e.g., oxo, C₁-C₆ alkyl, C₂-C₆alkenyl, C₂-C₆ alkynyl, C₁-C₃ alkoxy, OH, halogen, NH₂, NH(C₁-C₃ alkyl),N(C₁-C₃ alkyl)₂, and CN). In certain embodiments, the non-bulkysubstitution is located on the chain carbon atom proximal to the Degron(i.e., the carbon atom is separated from the carbon atom to which theDegron is bonded by at least 3, 4, or 5 chain atoms in the Linker).

In certain embodiments, the Linker is of Formula LO:

or an enantiomer, diastereomer, or stereoisomer thereof, wherein

p1 is an integer selected from 0 to 12;

p2 is an integer selected from 0 to 12;

p3 is an integer selected from 1 to 6;

each W is independently absent, CH₂, O, S, NH or NR₅;

Z is absent, CH₂, O, NH or NR₅;

each R₅ is independently C₁-C₃ alkyl; and

Q is absent or —CH₂C(O)NH—,

wherein the Linker is covalently bonded to the Degron with the

next to Q, and covalently bonded to the dTAG Targeting Ligand with the

next to Z, and wherein the total number of chain atoms in the Linker isless than 20.

In certain embodiments, the Linker-dTAG Targeting Ligand (TL) has thestructure of Formula L1 or L2:

or an enantiomer, diastereomer, or stereoisomer thereof, wherein:

p1 is an integer selected from 0 to 12;

p2 is an integer selected from 0 to 12;

p3 is an integer selected from 1 to 6;

each W is independently absent, CH₂, O, S, NH or NR₅;

Z is absent, CH₂, O, NH or NR₅;

each R₅ is independently C₁-C₃ alkyl; and

TL is a dTAG Targeting Ligand,

wherein the Linker is covalently bonded to the Degron with

In certain embodiments, p1 is an integer selected from 0 to 10.

In certain embodiments, p1 is an integer selected from 2 to 10.

In certain embodiments, p1 is selected from 1, 2, 3, 4, 5, and 6.

In certain embodiments, p1 is selected from 1, 3, and 5.

In certain embodiments, p1 is selected from 1, 2, and 3.

In certain embodiments, p1 is 3.

In certain embodiments, p2 is an integer selected from 0 to 10.

In certain embodiments, p2 is selected from 0, 1, 2, 3, 4, 5, and 6.

In certain embodiments, p2 is an integer selected from 0 and 1.

In certain embodiments, p3 is an integer selected from 1 to 5.

In certain embodiments, p3 is selected from 2, 3, 4, and 5.

In certain embodiments, p3 is selected from 1, 2, and 3.

In certain embodiments, p3 is selected from 2 and 3.

In certain embodiments, at least one W is CH₂.

In certain embodiments, at least one W is O.

In certain embodiments, at least one W is S.

In certain embodiments, at least one W is NH.

In certain embodiments, at least one W is NR₅; and R₅ is C₁-C₃ alkylselected from methyl, ethyl, and propyl.

In certain embodiments, W is O.

In certain embodiments, Z is absent.

In certain embodiments, Z is CH₂.

In certain embodiments, Z is O.

In certain embodiments, Z is NH.

In certain embodiments, Z is NR₅; and R₅ is C₁-C₃ alkyl selected frommethyl, ethyl, and propyl.

In certain embodiments, Z is part of the dTAG Targeting Ligand that isbonded to the Linker, namely, Z is formed from reacting a functionalgroup of the dTAG Targeting Ligand with the Linker.

In certain embodiments, W is CH₂, and Z is CH₂.

In certain embodiments, W is O, and Z is CH₂.

In certain embodiments, W is CH₂, and Z is O.

In certain embodiments, W is O, and Z is O.

In certain embodiments, the Linker-dTAG Targeting Ligand has thestructure selected from Table L:

TABLE L

wherein Z, TL, and p1 are each as described above.

Any one of the Degrons described herein can be covalently bound to anyone of the Linkers described herein.

In certain embodiments, the present application includes theDegron-Linker (DL) having the following structure:

wherein each of the variables is as described above in Formula DO andFormula LO, and a dTAG Targeting Ligand is covalently bonded to the DLwith the

next to Z.

In certain embodiments, the present application includes to theDegron-Linker (DL) having the following structure:

wherein each of the variables is as described above in Formula D andFormula LO, and a dTAG Targeting Ligand is covalently bonded to the DLwith the

next to Z.

Some embodiments of the present application relate to a bifunctionalcompound having the following structure:

or an enantiomer, diastereomer, or stereoisomer thereof, wherein each ofthe variables is as described above in Formula D and Formula LO, and thedTAG Targeting Ligand is described herein below.

Further embodiments of the present application relate to a bifunctionalcompound having the following structure:

or an enantiomer, diastereomer, or stereoisomer thereof, wherein each ofthe variables is as described above in Formula D and Formula LO, and thedTAG Targeting Ligand is described herein below.

Certain embodiments of the present application relate to bifunctionalcompounds having one of the following structures:

In certain embodiments, the Linker may be a polyethylene glycol groupranging in size from about 1 to about 12 ethylene glycol units, between1 and about 10 ethylene glycol units, about 2 about 6 ethylene glycolunits, between about 2 and 5 ethylene glycol units, between about 2 and4 ethylene glycol units.

In certain embodiments, the Linker is designed and optimized based onSAR (structure-activity relationship) and X-ray crystallography of thedTAG Targeting Ligand with regard to the location of attachment for theLinker.

In certain embodiments, the optimal Linker length and composition varyby target and can be estimated based upon X-ray structures of theoriginal dTAG Targeting Ligand bound to its target. Linker length andcomposition can be also modified to modulate metabolic stability andpharmacokinetic (PK) and pharmacodynamics (PD) parameters.

In certain embodiments, where the dTAG Targeting Ligand binds multipletargets, selectivity may be achieved by varying Linker length where theligand binds some of its targets in different binding pockets, e.g.,deeper or shallower binding pockets than others.

In an additional embodiment, the heterobifunctional compounds for use inthe present invention include a chemical Linker (L). In certainembodiments, the Linker group L is a group comprising one or morecovalently connected structural units of A (e.g., -A₁ . . . A_(q)-),wherein A₁ is a group coupled to at least one of a Degron, a dTAGTargeting Ligand, or a combination thereof.

In certain embodiments, A₁ links a Degron, a dTAG Targeting Ligand, or acombination thereof directly to another Degron, Targeting Ligand, orcombination thereof. In other embodiments, A₁ links a Degron, a dTAGTargeting Ligand, or a combination thereof indirectly to another Degron,dTAG Targeting Ligand or combination thereof through A_(q).

In certain embodiments, A₁ to A_(q) are, each independently, a bond,CR^(L1)R^(L2), O, S, SO, SO₂, NR^(L3), SO₂NR^(L3), SONR^(L3), CONR^(L3),NR^(L3)CONR^(L4), NR^(L3)SO₂NR^(L4), CO, CR^(L1)═CR^(L2), C≡C,SiR^(L1)R^(L2), P(O)R^(L1), P(O)OR^(L1), —NR^(L3)C(═NCN)NR^(L4),NR^(L3)C(═NCN), NR^(L3)C(═CNO₂)NR^(L4), C₃₋₁₁cycloalkyl optionallysubstituted with 0-6 R^(L1) and/or R^(L2) groups, C₃₋₁₁heteocyclyloptionally substituted with 0-6 R^(L1) and/or R^(L2) groups, aryloptionally substituted with 0-6 R^(L1) and/or R^(L2) groups, heteroaryloptionally substituted with 0-6 R^(L1) and/or R^(L2) groups, whereR^(L1) or R^(L2), each independently, can be linked to other A groups toform a cycloalkyl and/or heterocyclyl moiety which can be furthersubstituted with 0-4 R^(L5) groups; wherein

-   -   R^(L1), R^(L2), R^(L3), R^(L4) and R^(L5) are, each        independently, H, halo, C₁₋₈alkyl, OC₁₋₈alkyl, SC₁₋₈alkyl,        NHC₁₋₈alkyl, N(C₁₋₈alkyl)₂, C₃₋₁₁cycloalkyl, aryl, heteroaryl,        C₃₋₁₁heterocyclyl, OC₁₋₈cycloalkyl, SC₁₋₈cycloalkyl,        NHC₁₋₈cycloalkyl, N(C₁₋₈cycloalkyl)₂,        N(C₁₋₈cycloalkyl)(C₁₋₈alkyl), OH, NH₂, SH, SO₂C₁₋₈alkyl,        P(O)(OC₁₋₈alkyl)(C₁₋₈alkyl), P(O)(OC₁₋₈alkyl)₂, CC—C₁₋₈alkyl,        CCH, CH═CH(C₁₋₈alkyl), C(C₁₋₈alkyl)═CH(C₁₋₈alkyl),        C(C₁₋₈alkyl)═C(C₁₋₈alkyl)₂, Si(OH)₃, Si(C₁₋₈alkyl)₃,        Si(OH)(C₁₋₈alkyl)₂, COC₁₋₈alkyl, CO₂H, halogen, CN, CF₃, CHF₂,        CH₂F, NO₂, SF₅, SO₂NHC₁₋₈alkyl, SO₂N(C₁₋₈alkyl)₂, SONHC₁₋₈alkyl,        SON(C₁₋₈alkyl)₂, CONHC₁₋₈alkyl, CON(C₁₋₈alkyl)₂,        N(C₁₋₈alkyl)CONH(C₁₋₈alkyl), N(C₁₋₈alkyl)CON(C₁₋₈alkyl)₂,        NHCONH(C₁₋₈alkyl), NHCON(C₁₋₈alkyl)₂, NHCONH₂,        N(C₁₋₈alkyl)SO₂NH(C₁₋₈alkyl), N(C₁₋₈alkyl) SO₂N(C₁₋₈alkyl)₂, NH        SO₂NH(C₁₋₈alkyl), NH SO₂N(C₁₋₈alkyl)₂, NH SO₂NH₂.

In certain embodiments, q is an integer greater than or equal to 0. Incertain embodiments, q is an integer greater than or equal to 1.

In certain embodiments, e.g., where q is greater than 2, A_(q) is agroup which is connected to a Degron, and A₁ and A_(q) are connected viastructural units of A (number of such structural units of A: q−2).

In certain embodiments, e.g., where q is 2, A_(q) is a group which isconnected to A₁ and to a Degron moiety.

In certain embodiments, e.g., where q is 1, the structure of the Linkergroup L is -A₁-, and A₁ is a group which is connected to a Degron moietyand a dTAG Targeting Ligand moiety.

In additional embodiments, q is an integer from 1 to 100, 1 to 90, 1 to80, 1 to 70, 1 to 60, 1 to 50, 1 to 40, 1 to 30, 1 to 20, or 1 to 10.

In certain embodiments, the Linker (L) is selected from the structuresin FIG. 24.

In other embodiments the Linker (L) is selected from the structures inFIG. 25, wherein

In additional embodiments, the Linker group is optionally substituted(poly)ethyleneglycol having between 1 and about 100 ethylene glycolunits, between about 1 and about 50 ethylene glycol units, between 1 andabout 25 ethylene glycol units, between about 1 and 10 ethylene glycolunits, between 1 and about 8 ethylene glycol units and 1 and 6 ethyleneglycol units, between 2 and 4 ethylene glycol units, or optionallysubstituted alkyl groups interspersed with optionally substituted, O, N,S, P or Si atoms. In certain embodiments, the Linker is substituted withan aryl, phenyl, benzyl, alkyl, alkylene, or heterocycle group. Incertain embodiments, the Linker may be asymmetric or symmetrical.

In any of the embodiments of the compounds described herein, the Linkergroup may be any suitable moiety as described herein. In one embodiment,the Linker is a substituted or unsubstituted polyethylene glycol groupranging in size from about 1 to about 12 ethylene glycol units, between1 and about 10 ethylene glycol units, about 2 about 6 ethylene glycolunits, between about 2 and 5 ethylene glycol units, between about 2 and4 ethylene glycol units.

Although the Degron group and dTAG Targeting Ligand group may becovalently linked to the Linker group through any group which isappropriate and stable to the chemistry of the Linker, the Linker isindependently covalently bonded to the Degron group and the dTAGTargeting Ligand group preferably through an amide, ester, thioester,keto group, carbamate (urethane), carbon or ether, each of which groupsmay be inserted anywhere on the Degron group and dTAG Targeting Ligandgroup to provide maximum binding of the Degron group on the ubiquitinligase and the dTAG Targeting Ligand group on the target dTAG. (It isnoted that in certain aspects where the Degron group targets UbiquitinLigase, the target protein for degradation may be the ubiquitin ligaseitself). The Linker may be linked to an optionally substituted alkyl,alkylene, alkene or alkyne group, an aryl group or a heterocyclic groupon the Degron and/or dTAG Targeting Ligand groups.

In certain embodiments, “L” can be linear chains with linear atoms from4 to 24, the carbon atom in the linear chain can be substituted withoxygen, nitrogen, amide, fluorinated carbon, etc., such as thestructures in FIG. 26.

In certain embodiments, “L” can be nonlinear chains, and can bealiphatic or aromatic or heteroaromatic cyclic moieties, some examplesof “L” include but not be limited to the structures of FIG. 27, whereinX and Y are independently selected from a bond, CR^(L1)R^(L2), O, S, SO,SO₂, NR^(L3), SO₂NR^(L3), SONR^(L3), CONR^(L3), NR^(L3)CONR^(L4),NR^(L3)SO₂NR^(L4), CO, CR^(L1)═CR^(L2), C≡C, SiR^(L1)R^(L2), P(O)R^(L1),P(O)OR^(L1),-NR^(L3)C(═NCN)NR^(L4), NR^(L3)C(═NCN),NR^(L3)C(═CNO₂)NR^(L4), C₃₋₁₁cycloalkyl optionally substituted with 0-6R^(L1) and/or R^(L2) groups, C₃₋₁₁heteocyclyl optionally substitutedwith 0-6 R^(L1) and/or R^(L2) groups, aryl optionally substituted with0-6 R^(L1) and/or R^(L2) groups, heteroaryl optionally substituted with0-6 RL and/or R^(L2) groups, where R^(L1) or R^(L2), each independently,can be linked to other A groups to form a cycloalkyl and/or heterocyclylmoiety which can be further substituted with 0-4 R^(L5) groups.

dTAG Targeting Ligand

The dTAG Targeting Ligand (TL) is capable of binding to a dTAG or beingbound by a dTAG target that allows tagging with ubiquitin to occur;

As contemplated herein, the genomes of the present invention include aheterobifunctional compound targeted protein (dTAG) which locates in thecytoplasm. The heterobifunctional compound targeted protein of thegenome is any amino acid sequence to which a heterobifunctional compoundcan be bound, leading to the degradation of the protein-dTAG hybridprotein when in contact with the heterobifunctional compound.Preferably, the dTAG should not interfere with the function of the CAR.In one embodiment, the dTAG is a non-endogenous peptide, leading toheterobifunctional compound selectivity and allowing for the avoidanceof off target effects upon administration of the heterobifunctionalcompound. In one embodiment, the dTAG is an amino acid sequence derivedfrom an endogenous protein which has been modified so that theheterobifunctional compound binds only to the modified amino acidsequence and not the endogenously expressed protein. In one embodiment,the dTAG is an endogenously expressed protein. Any amino acid sequencedomain that can be bound by a ligand for use in a heterobifunctionalcompound can be used as a dTAG as contemplated herewith.

In particular embodiments, the dTAGs for use in the present inventioninclude, but are not limited to, amino acid sequences derived fromendogenously expressed proteins such as FK506 binding protein-12(FKBP12), bromodomain-containing protein 4 (BRD4), CREB binding protein(CREBBP), and transcriptional activator BRG1 (SMARCA4), or a variantthereof. As contemplated herein, “variant” means any variant such as asubstitution, deletion, or addition of one or a few to plural aminoacids, provided that the variant substantially retains the same functionas the original sequence, which in this case is providing ligand bindingfor a heterobifunctional compound. In other embodiments, dTAGs for us inthe present invention may include, for example, hormone receptors e.g.estrogen-receptor proteins, androgen receptor proteins, retinoid xreceptor (RXR) protein, and dihydrofolate reductase (DHFR), includingbacterial DHFR, bacterial dehydrogenase, and variants.

In one embodiment the dTAG is a portion of any of the proteinsidentified herein. For example, the dTAG can be the BD1 domain of BRD4or the BD2 domain of BRD4. In one embodiment that Targeting Ligandsidentified herein to target the parent dTAG are instead used to targetportion. In one embodiment, the BRD4 Targeting Ligands in Table T can beused to target the BD1 dTAG. In another embodiment, the BRD4 TargetingLigands in Table T can be used to target the BD2 dTAG.

Some embodiments of the present application include TLs which targetdTAGs including, but not limited to, those derived from Hsp90inhibitors, kinase inhibitors, MDM2 inhibitors, compounds targetingHuman BET bromodomain-containing proteins, compounds targeting cytosolicsignaling protein FKBP12, HDAC inhibitors, human lysinemethyltransferase inhibitors, angiogenesis inhibitors, immunosuppressivecompounds, and compounds targeting the aryl hydrocarbon receptor (AHR).

In certain embodiments, the dTAG Targeting Ligand is a compound that iscapable of binding to or binds to a dTAG derived from a kinase, a BETbromodomain-containing protein, a cytosolic signaling protein (e.g.,FKBP12), a nuclear protein, a histone deacetylase, a lysinemethyltransferase, a protein regulating angiogenesis, a proteinregulating immune response, an aryl hydrocarbon receptor (AHR), anestrogen receptor, an androgen receptor, a glucocorticoid receptor, or atranscription factor (e.g., SMARCA4, SMARCA2, TRIM24).

In certain embodiments, the dTAG is derived from a kinase to which thedTAG Targeting Ligand is capable of binding or binds including, but notlimited to, a tyrosine kinase (e.g., AATK, ABL, ABL2, ALK, AXL, BLK,BMX, BTK, CSF1R, CSK, DDR1, DDR2, EGFR, EPHA1, EPHA2, EPHA3, EPHA4,EPHA5, EPHA6, EPHA7, EPHA8, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB6,ERBB2, ERBB3, ERBB4, FER, FES, FGFR1, FGFR2, FGFR3, FGFR4, FGR, FLT1,FLT3, FLT4, FRK, FYN, GSG2, HCK, IGF1R, ILK, INSR, INSRR, IRAK4, ITK,JAK1, JAK2, JAK3, KDR, KIT, KSR1, LCK, LMTK2, LMTK3, LTK, LYN, MATK,MERTK, MET, MLTK, MST1R, MUSK, NPR1, NTRK1, NTRK2, NTRK3, PDGFRA,PDGFRB, PLK4, PTK2, PTK2B, PTK6, PTK7, RET, ROR1, ROR2, ROS1, RYK,SGK493, SRC, SRMS, STYK1, SYK, TEC, TEK, TEX14, TIE1, TNK1, TNK2,TNNI3K, TXK, TYK2, TYRO3, YES1, or ZAP70), a serine/threonine kinase(e.g., casein kinase 2, protein kinase A, protein kinase B, proteinkinase C, Raf kinases, CaM kinases, AKT1, AKT2, AKT3, ALK1, ALK2, ALK3,ALK4, Aurora A, Aurora B, Aurora C, CHK1, CHK2, CLK1, CLK2, CLK3, DAPK1,DAPK2, DAPK3, DMPK, ERK1, ERK2, ERK5, GCK, GSK3, HIPK, KHS1, LKB1, LOK,MAPKAPK2, MAPKAPK, MNK1, MSSK1, MST1, MST2, MST4, NDR, NEK2, NEK3, NEK6,NEK7, NEK9, NEK11, PAK1, PAK2, PAK3, PAK4, PAK5, PAK6, PIM1, PIM2, PLK1,RIP2, RIPS, RSK1, RSK2, SGK2, SGK3, SIK1, STK33, TAO1, TAO2, TGF-beta,TLK2, TSSK1, TSSK2, ULK1, or ULK2), a cyclin dependent kinase (e.g.,Cdk1-Cdk11), and a leucine-rich repeat kinase (e.g., LRRK2).

In certain embodiments, the dTAG is derived from a BETbromodomain-containing protein to which the dTAG Targeting Ligand iscapable of binding or binds including, but not limited to, ASH1L, ATAD2,BAZ1A, BAZ1B, BAZ2A, BAZ2B, BRD1, BRD2, BRD3, BRD4, BRD5, BRD6, BRD7,BRD8, BRD9, BRD10, BRDT, BRPF1, BRPF3, BRWD3, CECR2, CREBBP, EP300,FALZ, GCN5L2, KIAA1240, LOC93349, MLL, PB1, PCAF, PHIP, PRKCBP1,SMARCA2, SMARCA4, SP100, SP110, SP140, TAF1, TAF1L, TIFla, TRIM28,TRIM33, TRIM66, WDR9, ZMYND11, and MLL4. In certain embodiments, a BETbromodomain-containing protein is BRD4.

In certain embodiments, the dTAG is derived from a nuclear protein towhich the dTAG Targeting Ligand is capable of binding or bindsincluding, but not limited to, BRD2, BRD3, BRD4, AntennapediaHomeodomain Protein, BRCA1, BRCA2, CCAAT-Enhanced-Binding Proteins,histones, Polycomb-group proteins, High Mobility Group Proteins,Telomere Binding Proteins, FANCA, FANCD2, FANCE, FANCF, hepatocytenuclear factors, Mad2, NF-kappa B, Nuclear Receptor Coactivators,CREB-binding protein, p55, p107, p130, Rb proteins, p53, c-fos, c-jun,c-mdm2, c-myc, and c-rel.

In certain embodiments, the dTAG Targeting Ligand is selected from akinase inhibitor, a BET bromodomain-containing protein inhibitor,cytosolic signaling protein FKBP12 ligand, an HDAC inhibitor, a lysinemethyltransferase inhibitor, an angiogenesis inhibitor, animmunosuppressive compound, and an aryl hydrocarbon receptor (AHR)inhibitor.

In certain embodiments, the dTAG Targeting Ligand is a SERM (selectiveestrogen receptor modulator) or SERD (selective estrogen receptordegrader). Non-limiting examples of SERMs and SERDs are provided in WO2014/191726 assigned to Astra Zeneca, WO2013/090921, WO 2014/203129, WO2014/203132, and US2013/0178445 assigned to Olema Pharmaceuticals, andU.S. Pat. Nos. 9,078,871, 8,853,423, and 8,703,810, as well as US2015/0005286, WO 2014/205136, and WO 2014/205138 assigned to SeragonPharmaceuticals.

Additional dTAG Targeting Ligands include, for example, any moiety whichbinds to an endogenous protein (binds to a target dTAG). IllustrativedTAG Targeting Ligands includes the small molecule dTAG TargetingLigand: Hsp90 inhibitors, kinase inhibitors, HDM2 and MDM2 inhibitors,compounds targeting Human BET bromodomain-containing proteins, HDACinhibitors, human lysine methyltransferase inhibitors, angiogenesisinhibitors, nuclear hormone receptor compounds, immunosuppressivecompounds, and compounds targeting the aryl hydrocarbon receptor (AHR),among numerous others. Such small molecule target dTAG binding moietiesalso include pharmaceutically acceptable salts, enantiomers, solvatesand polymorphs of these compositions, as well as other small moleculesthat may target a dTAG of interest.

In some embodiments the dTAG Targeting Ligand is an Ubc9 SUMO E2 ligase5F6D targeting ligand including but not limited to those described in“Insights Into the Allosteric Inhibition of the SUMO E2 Enzyme Ubc9.” byHewitt, W. M., et. al. (2016) Angew. Chem. Int. Ed. Engl. 55: 5703-5707

In another embodiment the dTAG Targeting Ligand is a Tank1 targetingligand including but not limited to those described in “Structure ofhuman tankyrase 1 in complex with small-molecule inhibitors PJ34 andXAV939.” Kirby, C. A., Cheung, A., Fazal, A., Shultz, M. D., Stams, T,(2012) Acta Crystallogr., Sect. F 68: 115-118; and “Structure-EfficiencyRelationship of [1,2,4]Triazol-3-ylamines as Novel NicotinamideIsosteres that Inhibit Tankyrases.” Shultz, M. D., et al. (2013) J. Med.Chem. 56: 7049-7059.

In another embodiment the dTAG Targeting Ligand is a SH2 domain of pp60Src targeting ligand including but not limited to those described in“Requirements for Specific Binding of Low Affinity Inhibitor Fragmentsto the SH2 Domain of pp60Src Are Identical to Those for High AffinityBinding of Full Length Inhibitors” Gudrun Lange, et al., J. Med. Chem.2003, 46, 5184-5195.

In another embodiment the dTAG Targeting Ligand is a Sec7 domaintargeting ligand including but not limited to those described in “TheLysosomal Protein Saposin B Binds Chloroquine.” Huta, B. P., et al.,(2016) Chemmedchem 11: 277.

In another embodiment the dTAG Targeting Ligand is a Saposin-B targetingligand including but not limited to those described in “The structure ofcytomegalovirus immune modulator UL141 highlights structural Ig-foldversatility for receptor binding” I. Nemcovicova and D. M. Zajonc ActaCryst. (2014). D70, 851-862.

In another embodiment the dTAG Targeting Ligand is a Protein S100-A72OWS targeting ligand including but not limited to those described in“2WOS STRUCTURE OF HUMAN S100A7 IN COMPLEX WITH 2,6 ANS” DOI:10.2210/pdb2wos/pdb; and “Identification and Characterization of BindingSites on S100A7, a Participant in Cancer and Inflammation Pathways.”Leon, R., Murray, et al., (2009) Biochemistry 48: 10591-10600.

In another embodiment the dTAG Targeting Ligand is a Phospholipase A2targeting ligand including but not limited to those described in“Structure-based design of the first potent and selective inhibitor ofhuman non-pancreatic secretory phospholipase A2” Schevitz, R. W., etal., Nat. Struct. Biol. 1995, 2, 458-465.

In another embodiment the dTAG Targeting Ligand is a PHIP targetingligand including but not limited to those described in “A PoisedFragment Library Enables Rapid Synthetic Expansion Yielding the FirstReported Inhibitors of PHIP(2), an Atypical Bromodomain” Krojer, T.; etal. Chem. Sci. 2016, 7, 2322-2330.

In another embodiment the dTAG Targeting Ligand is a PDZ targetingligand including but not limited to those described in “Discovery ofLow-Molecular-Weight Ligands for the AF6 PDZ Domain” Mangesh Joshi, etal. Angew. Chem. Int. Ed. 2006, 45, 3790-3795.

In another embodiment the dTAG Targeting Ligand is a PARP15 targetingligand including but not limited to those described in “Structural Basisfor Lack of ADP-ribosyltransferase Activity in Poly(ADP-ribose)Polymerase-13/Zinc Finger Antiviral Protein.” Karlberg, T., et al.,(2015) J. Biol. Chem. 290: 7336-7344.

In another embodiment the dTAG Targeting Ligand is a PARP14 targetingligand including but not limited to those described in “Discovery ofLigands for ADP-Ribosyltransferases via Docking-Based VirtualScreening.” Andersson, C. D., et al., (2012) J. Med. Chem. 55:7706-7718.; “Family-wide chemical profiling and structural analysis ofPARP and tankyrase inhibitors.” Wahlberg, E., et al. (2012) Nat.Biotechnol. 30: 283-288.; “Discovery of Ligands forADP-Ribosyltransferases via Docking-Based Virtual Screening.” Andersson,C. D., et al. (2012) J. Med. Chem. 55: 7706-7718.

In another embodiment the dTAG Targeting Ligand is a MTH1 targetingligand including but not limited to those described in “MTH1 inhibitioneradicates cancer by preventing sanitation of the dNTP pool” Helge Gad,et. al. Nature, 2014, 508, 215-221.

In another embodiment the dTAG Targeting Ligand is a mPGES-1 targetingligand including but not limited to those described in “CrystalStructures of mPGES-1 Inhibitor Complexes Form a Basis for the RationalDesign of Potent Analgesic and Anti-Inflammatory Therapeutics.” Luz, J.G., et al., (2015) J. Med. Chem. 58: 4727-4737.

In another embodiment the dTAG Targeting Ligand is aFLAP-5-lipoxygenase-activating protein targeting ligand including butnot limited to those described in “Crystal structure of inhibitor-boundhuman 5-lipoxygenase-activating protein.” Ferguson, A. D., McKeever, B.M., Xu, S., Wisniewski, D., Miller, D. K., Yamin, T. T., Spencer, R. H.,Chu, L., Ujjainwalla, F., Cunningham, B. R., Evans, J. F., Becker, J. W.(2007) Science 317: 510-512.

In another embodiment the dTAG Targeting Ligand is a FA Binding Proteintargeting ligand including but not limited to those described in “AReal-World Perspective on Molecular Design.” Kuhn, B.; et al. J. Med.Chem. 2016, 59, 4087-4102.

In another embodiment the dTAG Targeting Ligand is a BCL2 targetingligand including but not limited to those described in “ABT-199, apotent and selective BCL-2 inhibitor, achieves antitumor activity whilesparing platelets.” Souers, A. J., et al. (2013) NAT. MED. (N.Y.) 19:202-208.

In another embodiment the dTAG Targeting Ligand is an EGFR targetingligand. In one embodiment the dTAG Targeting Ligand is selected fromerlotinib (Tarceva), gefitinib (Iressa), afatinib (Gilotrif),rociletinib (CO-1686), osimertinib (Tagrisso), olmutinib (Olita),naquotinib (ASP8273), nazartinib (EGF816), PF-06747775 (Pfizer),icotinib (BPI-2009), neratinib (HKI-272; PB272); avitinib (AC0010),EAI045, tarloxotinib (TH-4000; PR-610), PF-06459988 (Pfizer),tesevatinib (XL647; EXEL-7647; KD-019), transtinib, WZ-3146, WZ8040,CNX-2006, and dacomitinib (PF-00299804; Pfizer). The linker can beplaced on these Targeting Ligands in any location that does notinterfere with the Ligands binding to EGFR. Non-limiting examples ofLinker binding locations are provided in Table T below. In oneembodiment the EGFR targeting ligand binds the L858R mutant of EGFR. Inanother embodiment the EGFR targeting ligand binds the T790M mutant ofEGFR. In another embodiment the EGFR targeting ligand binds the C797G orC797S mutant of EGFR. In one embodiment the EGFR targeting ligand isselected from erlotinib, gefitinib, afatinib, neratinib, and dacomitiniband binds the L858R mutant of EGFR. In another embodiment the EGFRtargeting ligand is selected from osimertinib, rociletinib, olmutinib,naquotinib, nazartinib, PF-06747775, Icotinib, Neratinib, Avitinib,Tarloxotinib, PF-0645998, Tesevatinib, Transtinib, WZ-3146, WZ8040, andCNX-2006 and binds the T790M mutant of EGFR. In another embodiment theEGFR targeting ligand is EA1045 and binds the C797G or C797S mutant ofEGFR.

Any protein which can bind to a dTAG Targeting Ligand group and acted onor degraded by a ubiquitin ligase is a target protein according to thepresent invention. In general, an endogenous target proteins for use asdTAGs may include, for example, structural proteins, receptors, enzymes,cell surface proteins, proteins pertinent to the integrated function ofa cell, including proteins involved in catalytic activity, aromataseactivity, motor activity, helicase activity, metabolic processes(anabolism and catabolism), antioxidant activity, proteolysis,biosynthesis, proteins with kinase activity, oxidoreductase activity,transferase activity, hydrolase activity, lyase activity, isomeraseactivity, ligase activity, enzyme regulator activity, signal transduceractivity, structural molecule activity, binding activity (protein, lipidcarbohydrate), receptor activity, cell motility, membrane fusion, cellcommunication, regulation of biological processes, development, celldifferentiation, response to stimulus, behavioral proteins, celladhesion proteins, proteins involved in cell death, proteins involved intransport (including protein transporter activity, nuclear transport,ion transporter activity, channel transporter activity, carrieractivity, permease activity, secretion activity, electron transporteractivity, pathogenesis, chaperone regulator activity, nucleic acidbinding activity, transcription regulator activity, extracellularorganization and biogenesis activity, translation regulator activity.

More specifically, a number of drug targets for human therapeuticsrepresent dTAG targets to which protein target or dTAG Targeting Ligandmay be bound and incorporated into compounds according to the presentinvention. These include proteins which may be used to restore functionin numerous polygenic diseases, including for example B7.1 and B7,TINFR1m, TNFR2, NADPH oxidase, BclIBax and other partners in theapoptosis pathway, C5a receptor, HMG-CoA reductase, PDE Vphosphodiesterase type, PDE IV phosphodiesterase type 4, PDE I, PDEII,PDEIII, squalene cyclase inhibitor, CXCR1, CXCR2, nitric oxide (NO)synthase, cyclo-oxygenase 1, cyclo-oxygenase 2, 5HT receptors, dopaminereceptors, G Proteins, i.e., Gq, histamine receptors, 5-lipoxygenase,tryptase serine protease, thymidylate synthase, purine nucleosidephosphorylase, GAPDH trypanosomal, glycogen phosphorylase, Carbonicanhydrase, chemokine receptors, JAW STAT, RXR and similar, HIV 1protease, HIV 1 integrase, influenza, neuramimidase, hepatitis B reversetranscriptase, sodium channel, multi drug resistance (MDR), proteinP-glycoprotein (and MRP), tyrosine kinases, CD23, CD124, tyrosine kinasep56 lck, CD4, CD5, IL-2 receptor, IL-1 receptor, TNF-alphaR, ICAM1, Cat+channels, VCAM, VLA-4 integrin, selectins, CD40/CD40L, newokinins andreceptors, inosine monophosphate dehydrogenase, p38 MAP Kinase,RaslRaflMEWERK pathway, interleukin-1 converting enzyme, caspase, HCV,NS3 protease, HCV NS3 RNA helicase, glycinamide ribonucleotide formyltransferase, rhinovirus 3C protease, herpes simplex virus-1 (HSV-I),protease, cytomegalovirus (CMV) protease, poly (ADP-ribose) polymerase,cyclin dependent kinases, vascular endothelial growth factor, oxytocinreceptor, microsomal transfer protein inhibitor, bile acid transportinhibitor, 5 alpha reductase inhibitors, angiotensin 11, glycinereceptor, noradrenaline reuptake receptor, endothelin receptors,neuropeptide Y and receptor, estrogen receptors, androgen receptors,adenosine receptors, adenosine kinase and AMP deaminase, purinergicreceptors (P2Y1, P2Y2, P2Y4, P2Y6, P2X1-7), farnesyltransferases,geranylgeranyl transferase, TrkA a receptor for NGF, beta-amyloid,tyrosine kinase Flk-IIKDR, vitronectin receptor, integrin receptor,Her-21 neu, telomerase inhibition, cytosolic phospholipaseA2 and EGFreceptor tyrosine kinase. Additional protein targets useful as dTAGsinclude, for example, ecdysone 20-monooxygenase, ion channel of the GABAgated chloride channel, acetylcholinesterase, voltage-sensitive sodiumchannel protein, calcium release channel, and chloride channels. Stillfurther target proteins for use as dTAGs include Acetyl-CoA carboxylase,adenylosuccinate synthetase, protoporphyrinogen oxidase, andenolpyruvylshikimate-phosphate synthase.

In one embodiment the dTAG and dTAG Targeting Ligand pair are chosen byscreening a library of ligands. Such a screening is exemplified in“Kinase Inhibitor Profiling Reveals Unexpected Opportunities to InhibitDisease-Associated Mutant Kinases” by Duong-Ly et al.; Cell Reports 14,772-781 Feb. 2, 2016.

Haloalkane dehalogenase enzymes are another target of specific compoundsaccording to the present invention which may be used as dTAGs. Compoundsaccording to the present invention which contain chloroalkane peptidebinding moieties (C1-C12 often about C2-C10 alkyl halo groups) may beused to inhibit and/or degrade haloalkane dehalogenase enzymes which areused in fusion proteins or related diagnostic proteins as described inPCT/US2012/063401 filed Dec. 6, 2011 and published as WO 2012/078559 onJun. 14, 2012, the contents of which is incorporated by referenceherein.

Non-limiting examples of dTAG Targeting Ligands are shown below in TableT and represent dTAG Targeting Ligands capable of targeting proteins oramino acid sequence useful as dTAGs.

TABLE T A. BRD dTAG Targeting Ligands: BRD dTAG Targeting Ligands asused herein include, but are not limited to:

wherein: R is the point at which the Linker is attached; and R′: ismethyl or ethyl. B. CREBBP dTAG Targeting Ligands: CREBBP dTAG TargetingLigands as used herein include, but are not limited to:

wherein: R is the point at which the Linker is attached; A is N or CH;and m is 0, 1, 2, 3, 4, 5, 6, 7, or 8. C. SMARCA4, PB1, and/or SMARCA2dTAG Targeting Ligands: SMARCA4, PB1, and/or SMARCA2 dTAG TargetingLigands as used herein include, but are not limited to:

wherein: R is the point at which the Linker is attached; A is N or CH;and m is 0, 1, 2, 3, 4, 5, 6, 7, or 8. D. TRIM24 and/or BRPF1 dTAGTargeting Ligands: TRIM24 and/or BRPF1 dTAG Targeting Ligands as usedherein include, but are not limited to:

wherein: R is the point at which the Linker is attached; and m is 0, 1,2, 3, 4, 5, 6, 7, or 8. E. Glucocorticoid Receptor dTAG TargetingLigand: Glucocorticoid dTAG Targeting Ligands as used herein include,but are not limited to:

wherein: R is the point at which the Linker is attached. F. Estrogenand/or Androgen Receptor dTAG Targeting Ligands: Estrogen and/orAndrogen dTAG Targeting Ligands as used herein include, but are notlimited to:

wherein: R is the point at which the Linker is attached. G. DOT1L dTAGTargeting Ligands: DOT1L dTAG Targeting Ligands as used herein include,but are not limited to:

wherein: R is the point at which the Linker is attached; A is N or CH;and m is 0, 1, 2, 3, 4, 5, 6, 7, or 8. H. Ras dTAG Targeting Ligands:Ras dTAG Targeting Ligands as used herein include, but are not limitedto:

wherein: R is the point at which the Linker is attached. I. RasG12C dTAGTargeting Ligands: RasG12C dTAG Targeting Ligands as used hereininclude, but are not limited to:

wherein: R is the point at which the Linker is attached. J. Her3 dTAGTargeting Ligands: Her3 dTAG Targeting Ligands as used herein include,but are not limited to:

wherein: R is the point at which the Linker is attached; and

K. Bcl-2 or Bcl-XL dTAG Targeting Ligands: Bcl-2 or Bcl-XL dTAGTargeting Ligands as used herein include, but are not limited to:

wherein: R is the point at which the Linker is attached. L. HDAC dTAGTargeting Ligands: HDAC dTAG Targeting Ligands as used herein include,but are not limited to:

wherein: R is the point at which the Linker is attached. M. PPAR-gammadTAG Targeting Ligands: PPAR-gamma dTAG Targeting Ligands as used hereininclude, but are not limited to:

wherein: R is the point at which the Linker is attached. N. RXR dTAGTargeting Ligands: RXR dTAG Targeting Ligands as used herein include,but are not limited to:

wherein: R is the point at which the Linker is attached. O. DHFR dTAGTargeting Ligands: DHFR dTAG Targeting Ligands as used herein include,but are not limited to:

wherein: R is the point at which the Linker is attached. P. EGFR dTAGTargeting Ligands: EGFR dTAG Targeting Ligands as used herein include,but are not limited to: 1. Targeting Ligands that target L858R mutantEGFR, including erlotinib, gefitnib, afatinib, neratinib, anddacomitinib.

wherein: R is the point at which the Linker is attached. 2. TargetingLigands that target T790M mutant EGFR, including osimertinib,rociletinib, olmutinib, naquotinib, nazartinib, PF-06747775, Icotinib,Neratinib, Avitinib, Tarloxotinib, PF- 0645998, Tesevatinib, Transtinib,WZ-3146, WZ8040, and CNX-2006:

wherein: R is the point at which the Linker is attached. 3. TargetingLigands that target C797S mutant EGFR, including EAI045:

wherein: R is the point at which the Linker is attached. Q. BCR-ABL dTAGTargeting Ligands: BCR-ABL dTAG Targeting Ligands as used hereininclude, but are not limited to: 1. Targeting Ligands that target T315Imutant BCR-ABL (PDB #3CS9), including Nilotinib and Dasatinib:

wherein: R is the point at which the Linker is attached. 2. TargetingLigands that target BCR-ABL, including Nilotinib, Dasatinib, Ponatinib,and Bosutinib:

wherein: R is the point at which the Linker is attached. R. ALK dTAGTargeting Ligands: ALK dTAG Targeting Ligands as used herein include,but are not limited to: 1. Targeting Ligands that target L1196M mutantALK (PDB #4MKC), including Ceritinib:

wherein: R is the point at which the Linker is attached. S. JAK2 dTAGTargeting Ligands: JAK2 dTAG Targeting Ligands as used herein include,but are not limited to: 1. Targeting Ligands that target V617F mutantJAK2, including Ruxolitinib:

wherein: R is the point at which the Linker is attached. T. BRAF dTAGTargeting Ligands: BRAF dTAG Targeting Ligands as used herein include,but are not limited to: 1. Targeting Ligands that target V600E mutantBRAF (PBD #3OG7), including Vemurafenib:

wherein: R is the point at which the Linker is attached. 2. TargetingLigands that target BRAF, including Dabrafenib:

wherein: R is the point at which the Linker is attached. U. LRRK2 dTAGTargeting Ligands: LRRK2 dTAG Targeting Ligands as used herein include,but are not limited to: 1. Targeting Ligands that target R1441C mutantLRRK2, including:

wherein: R is the point at which the Linker is attached. 2. TargetingLigands that target G2019S mutant LRRK2, including:

wherein: R is the point at which the Linker is attached. 3. TargetingLigands that target I2020T mutant LRRK2, including:

wherein: R is the point at which the Linker is attached. V. PDGFRα dTAGTargeting Ligands: PDGFRα dTAG Targeting Ligands as used herein include,but are not limited to: 1. Targeting Ligands that target T674I mutantPDGFRα, including AG-1478, CHEMBL94431, Dovitinib, erlotinib, gefitinib,imatinib, Janex 1, Pazopanib, PD153035, Sorafenib, Sunitinib, WHI-P180:

wherein: R is the point at which the Linker is attached. W. RET dTAGTargeting Ligands: RET dTAG Targeting Ligands as used herein include,but are not limited to: 1. Targeting Ligands that target G691S mutantRET, including tozasertib

wherein: R is the point at which the Linker is attached. 2. TargetingLigands that target R749T mutant RET, including tozasertib

wherein: R is the point at which the Linker is attached. 3. TargetingLigands that target E762Q mutant RET, including tozasertib

wherein: R is the point at which the Linker is attached. 4. TargetingLigands that target Y791F mutant RET, including tozasertib

wherein: R is the point at which the Linker is attached. 5. TargetingLigands that target V804M mutant RET, including tozasertib

wherein: R is the point at which the Linker is attached. 6. TargetingLigands that target M918T mutant RET, including tozasertib

wherein: R is the point at which the Linker is attached. X. Heat ShockProtein 90 (HSP90) dTAG Targeting Ligands: Heat Shock Protein 90 (HSP90)dTAG Targeting Ligands as used herein include, but are not limitedto: 1. The HSP90 inhibitors identified in Vallee, et al., “TricyclicSeries of Heat Shock Protein 90 (HSP90) Inhibitors Part I: Discovery ofTricyclic Imidazo[4,5-C]Pyridines as Potent Inhibitors of the HSP90Molecular Chaperone (2011) J. Med. Chem. 54: 7206, including YKB(N-[4-(3H- imidazo[4,5-C]Pyridin-2-yl)-9H-Fluoren-9-yl]-succinamide):

derivatized where a Linker group L or a —(L-DEGRON) group is attached,for example, via the terminal amide group; 2. The HSP90 inhibitor p54(modified) (8-[(2,4-dimethylphenyl)sulfanyl]-3]pent-4-yn-1-yl-3H-purin-6-amine):

derivatized where a Linker group L or a —(L-DEGRON) group is attached,for example, via the terminal acetylene group; 3. The HSP90 inhibitors(modified) identified in Brough, et al., “4,5-Diarylisoxazole HSP90Chaperone Inhibitors: Potential Therapeutic Agents for the Treatment ofCancer”, J. MED. CHEM. vol: 51, page: 196 (2008), including the compound2GJ (5-[2,4-dihydroxy-5-(1-methylethyl)phenyl]-n-ethyl-4-[4-(morpholin-4-ylmethyl)phenyl]isoxazole-3-carboxamide)having the structure:

derivatized, where a Linker group L or a —(L-DEGRON) group is attached,for example, via the amide group (at the amine or at the alkyl group onthe amine); 4. The HSP90 inhibitors (modified) identified in Wright, etal., Structure-Activity Relationships in Purine-Based Inhibitor Bindingto HSP90 Isoforms, Chem Biol. 2004 June; 11(6):775-85, including theHSP90 inhibitor PU3 having the structure:

derivatized where a Linker group L or —(L-DEGRON) is attached, forexample, via the butyl group; and 5. The HSP90 inhibitor geldanamycin((4E,6Z,8S,9S,10E,12S,13R,14S,16R)-13-hydroxy-8,14,19-trimethoxy-4,10,12,16-tetramethyl-3,20,22-trioxo-2-azabicyclo[16.3.1](derivatized)or any of its derivatives (e.g. 17-alkylamino-17-desmethoxygeldanamycin(“17-AAG”) or 17-(2- dimethylaminoethyl)amino-17-desmethoxygeldanamycin(“17-DMAG”)) (derivatized, where a Linker group L or a —(L-DEGRON) groupis attached, for example, via the amide group). Y. Kinase andPhosphatase dTAG Targeting Ligands: Kinase and Phosphatase dTAGTargeting Ligands as used herein include, but are not limited to: 1.Erlotinib Derivative Tyrosine Kinase Inhibitor:

where R is a Linker group L or a —(L-DEGRON) group attached, forexample, via the ether group; 2. The kinase inhibitor sunitinib(derivatized):

derivatized where R is a Linker group L or a —(L-DEGRON) group attached,for example, to the pyrrole moiety; 3. Kinase Inhibitor sorafenib(derivatized):

derivatized where R is a Linker group L or a —(L-DEGRON) group attached,for example, to the amide moiety; 4. The kinase inhibitor desatinib(derivatized):

derivatized where R is a Linker group L or a —(L-DEGRON) attached, forexample, to the pyrimidine; 5. The kinase inhibitor lapatinib(derivatized):

derivatized where a Linker group L or a —(L-DEGRON) group is attached,for example, via the terminal methyl of the sulfonyl methyl group; 6.The kinase inhibitor U09-CX-5279 (derivatized):

derivatized where a Linker group L or a —(L-DEGRON) group is attached,for example, via the amine (aniline), carboxylic acid or amine alpha tocyclopropyl group, or cyclopropyl group; 7. The kinase inhibitorsidentified in Millan, et al., Design and Synthesis of Inhaled P38Inhibitors for the Treatment of Chronic Obstructive Pulmonary Disease,J. MED. CHEM. vol:54, page: 7797 (2011), including the kinase inhibitorsY1W and Y1X (Derivatized) having the structures:

YIX(1-ethyl-3-(2-{[3-(1-methylethyl)[1,2,4]triazolo[4,3-a]pyridine-6-yl]sulfanyl}benzyl)urea,derivatized where a Linker group L or a —(L-DEGRON) group is attached,for example, via the i- propyl group;

YIW1-(3-tert-butyl-1-phenyl-1H-pyrazol-5-yl)-3-(2-{[3-(1-methylethyl)[1,2,4]triazolo[4,3-a]pyridin-6-yl]sulfanyl}benzyl)urea derivatized where a Linker group L or a—(L-DEGRON) group is attached, for example, preferably via either thei-propyl group or the t-butyl group; 8. The kinase inhibitors identifiedin Schenkel, et al., Discovery of Potent and Highly SelectiveThienopyridine Janus Kinase 2 Inhibitors J. Med. Chem., 2011, 54 (24),pp 8440-8450, including the compounds 6TP and 0TP (Derivatized) havingthe structures:

6TP4-amino-2-[4-(tert-butylsulfamoyl)phenyl]-N-methylthieno[3,2-c]pyridine-7-carboxamideThienopyridine 19 derivatized where a Linker group L or a —(L-DEGRON)group is attached, for example, via the terminal methyl group bound toamide moiety;

0TP4-amino-N-methyl-2-[4-(morpholin-4-yl)phenyl]thieno[3,2-c]pyridine-7-carboxamideThienopyridine 8 derivatized where a Linker group L or a —(L-DEGRON)group is attached, for example, via the terminal methyl group bound tothe amide moiety; 9. The kinase inhibitors identified in Van Eis, etal., “2,6-Naphthyridines as potent and selective inhibitors of the novelprotein kinase C isozymes”, Biorg. Med. Chem. Lett. 2011 Dec. 15;21(24):7367-72, including the kinase inhibitor 07U having the structure:

07U 2-methyl-N^(~)1^(~)[3-(pyridin-4-yl)-2,6-naphthyridin-1-yl]propane-1,2-diaminederivatized where a Linker group L or a —(L-DEGRON) group is attached,for example, via the secondary amine or terminal amino group; 10. Thekinase inhibitors identified in Lountos, et al., “StructuralCharacterization of Inhibitor Complexes with Checkpoint Kinase 2 (Chk2),a Drug Target for Cancer Therapy”, J. STRUCT. BIOL. vol:176, pag: 292(2011), including the kinase inhibitor YCF having the structure:

derivatized where a Linker group L or a —(L-DEGRON) group is attached,for example, via either of the terminal hydroxyl groups; 11. The kinaseinhibitors identified in Lountos, et al., “Structural Characterizationof Inhibitor Complexes with Checkpoint Kinase 2 (Chk2), a Drug Targetfor Cancer Therapy”, J. STRUCT. BIOL. vol:176, pag: 292 (2011),including the kinase inhibitors XK9 and NXP (derivatized) having thestructures:

XK9N-{4-[(1E)-N-(N-hydroxycarbamimidoyl)ethanehydrazonoyl]phenyl}-7-nitro-1H-indole-2-carboxamide

NXP N-{4-[(1E)-N-CARBAMIMIDOYLETHANEHYDRAZONOYL]PHENYL}-1H-INDOLE-3-CARBOXAMIDE derivatized where a Linker group L or a —(L-DEGRON) group isattached, for example, via the terminal hydroxyl group (XK9) or thehydrazone group (NXP); 12. The kinase inhibitor afatinib (derivatized)(N-[4-[(3-chloro-4-fluorophenyl)amino]-7-[[(3S)-tetrahydro-3-furanyl]oxy]-6-quinazolinyl]-4(dimethylamino)-2-butenamide)(Derivatized where a Linker group L or a —(L-DEGRON) group is attached,for example, via the aliphatic amine group); 13. The kinase inhibitorfostamatinib (derivatized) ([6-({5-fluoro-2-[(3,4,5-trimethoxyphenyl)amino]pyrimidin-4-yl}amino)-2,2-dimethyl-3-oxo-2,3-dihydro-4H-pyrido[3,2-b]-1,4-oxazin-4-yl]methyl disodium phosphate hexahydrate)(Derivatized where a Linker group L or a —(L-DEGRON) group is attached,for example, via a methoxy group); 14. The kinase inhibitor gefitinib(derivatized) (N-(3-chloro-4-fluoro-phenyl)-7-methoxy-6-(3-morpholin-4-ylpropoxy)quinazolin-4-amine):

derivatized where a Linker group L or a —(L-DEGRON) group is attached,for example, via a methoxy or ether group; 15. The kinase inhibitorlenvatinib (derivatized) (4-[3-chloro-4-(cyclopropylcarbamoylamino)phenoxy]-7-methoxy-quinoline-6-carboxamide)(derivatized where a Linker group L or a —(L-DEGRON) group is attached,for example, via the cyclopropyl group); 16. The kinase inhibitorvandetanib (derivatized) (N-(4-bromo-2-fluorophenyl)-6-methoxy-7-[1-methylpiperidin-4-yl)methoxy]quinazolin-4-amine) (derivatized where aLinker group L or a —(L- DEGRON) group is attached, for example, via themethoxy or hydroxyl group); 17. The kinase inhibitor vemurafenib(derivatized) (propane-1-sulfonic acid {3-[5-(4-chlorophenyl)-1H-pyrrolo[2,3-b]pyridine-3-carbonyl]-2,4-difluoro-phenyl}-amide),derivatized where a Linker group L or a —(L-DEGRON) group is attached,for example, via the sulfonyl propyl group; 18. The kinase inhibitorGleevec (derivatized):

derivatized where R as a Linker group L or a —(L-DEGRON) group isattached, for example, via the amide group or via the aniline aminegroup; 19. The kinase inhibitor pazopanib (derivatized) (VEGFR3inhibitor):

derivatized where R is a Linker group L or a —(L-DEGRON) group attached,for example, to the phenyl moiety or via the aniline amine group; 20.The kinase inhibitor AT-9283 (Derivatized) Aurora Kinase Inhibitor

where R is a Linker group L or a —(L-DEGRON) group attached, forexample, to the phenyl moiety); 21. The kinase inhibitor TAE684(derivatized) ALK inhibitor

where R is a Linker group L or a —(L-DEGRON) group attached, forexample, to the phenyl moiety); 22. The kinase inhibitor nilotanib(derivatized) Abl inhibitor:

derivatized where R is a Linker group L or a —(L-DEGRON) group attached,for example, to the phenyl moiety or the aniline amine group; 23. KinaseInhibitor NVP-BSK805 (derivatized) JAK Inhibitor

derivatized where R is a Linker group L or a —(L-DEGRON) group attached,for example, to the phenyl moiety or the diazole group; 24. KinaseInhibitor crizotinib Derivatized Alk Inhibitor

derivatized where R is a Linker group L or a —(L-DEGRON) group attached,for example, to the phenyl moiety or the diazole group; 25. KinaseInhibitor JNJ FMS (derivatized) Inhibitor

derivatized where R is a Linker group L or a —(L-DEGRON) group attached,for example, to the phenyl moiety; 26. The kinase inhibitor foretinib(derivatized) Met Inhibitor

derivatized where R is a Linker group L or a —(L-DEGRON) group attached,for example, to the phenyl moiety or a hydroxyl or ether group on thequinoline moiety; 27. The allosteric Protein Tyrosine PhosphataseInhibitor PTP1B (derivatized):

derivatized where a Linker group L or a —(L-DEGRON) group is attached,for example, at R, as indicated; 28. The inhibitor of SHP-2 Domain ofTyrosine Phosphatase (derivatized):

derivatized where a Linker group L or a —(L-DEGRON) group is attached,for example, at R; 29. The inhibitor (derivatized) of BRAF(BRAFV600E)/MEK:

derivatized where a Linker group L or a —(L-DEGRON) group is attached,for example, at R; 30. Inhibitor (derivatized) of Tyrosine Kinase ABL

derivatized where where a Linker group L or a —(L-DEGRON) group isattached, for example, at R; 31. The kinase inhibitor OSI-027(derivatized) mTORC1/2 inhibitor

derivatized where a Linker group L or a —(L-DEGRON) group is attached,for example, at R; 32. The kinase inhibitor OSI-930 (derivatized)c-Kit/KDR inhibitor

derivatized where a Linker group L or a —(L-DEGRON) group is attached,for example, at R; and 33. The kinase inhibitor OSI-906 (derivatized)IGF1R/IR inhibitor

derivatized where a Linker group L or a —(L-DEGRON) group is attached,for example, at R. Wherein, in any of the embodiments described insections I-XVII, “R” designates a site for attachment of a Linker groupL or a —(L-DEGRON) group on the piperazine moiety. Z. HDM2 and/or MDM2dTAG Targeting Ligands: HDM2 and/or MDM2 dTAG Targeting Ligands as usedherein include, but are not limited to: 1. The HDM2/MDM2 inhibitorsidentified in Vassilev, et al., In vivo activation of the p53 pathway bysmall-molecule antagonists of MDM2, SCIENCE vol:303, pag: 844-848(2004), and Schneekloth, et al., Targeted intracellular proteindegradation induced by a small molecule: En route to chemicalproteomics, Bioorg. Med. Chem. Lett. 18 (2008) 5904-5908, including (oradditionally) the compounds nutlin-3, nutlin-2, and nutlin-1(derivatized) as described below, as well as all derivatives and analogsthereof:

(derivatized where a Linker group L or a —(L-DEGRON) group is attached,for example, at the methoxy group or as a hydroxyl group);

(derivatized where a Linker group L or a —(L-DEGRON) group is attached,for example, at the methoxy group or hydroxyl group);

(derivatized where a Linker group L or a —(L-DEGRON) group is attached,for example, via the methoxy group or as a hydroxyl group); and 2.Trans-4-Iodo-4′-Boranyl-Chalcone

(derivatized where a Linker group L or a Linker group L or a —(L-DEGRON)group is attached, for example, via a hydroxy group). AA. Human BETBromodomain-Containing Proteins dTAG Targeting Ligands: In certainembodiments, “dTAG Targeting Ligand” can be ligands binding to Bromo-and Extra-terminal (BET) proteins BRD2, BRD3 and BRD4. Compoundstargeting Human BET Bromodomain-containing proteins include, but are notlimited to the compounds associated with the targets as described below,where “R” or “Linker” designates a site for Linker group L or a—(L-DEGRON) group attachment, for example: 1. JQ1, Filippakopoulos etal. Selective inhibition of BET bromodomains. Nature (2010):

2. I-BET, Nicodeme et al. Suppression of Inflammation by a SyntheticHistone Mimic. Nature (2010). Chung et al. Discovery andCharacterization of Small Molecule Inhibitors of the BET FamilyBromodomains. J. Med Chem. (2011):

3. Compounds described in Hewings et al. 3,5-Dimethylisoxazoles Act asAcetyl-lysine Bromodomain Ligands. J Med. Chem (2011) 54 6761-6770.

4. I-BET151, Dawson et al. Inhibition of BET Recruitment to Chromatin asan Effective Treatment for MLL-fusion Leukemia. Nature (2011):

5. Carbazole type (US 2015/0256700)

6. Pyrrolopyridone type (US 2015/0148342)

7. Tetrahydroquinoline type (WO 2015/074064)

8. Triazolopyrazine type (WO 2015/067770)

9. Pyridone type (WO 2015/022332)

10. Quinazolinone type (WO 2015/015318)

11. Dihydropyridopyrazinone type (WO 2015/011084)

(Where R or L or Linker, in each instance, designates a site forattachment, for example, of a Linker group L or a —(L-DEGRON) group).BB. HDAC dTAG Targeting Ligands: HDAC dTAG Targeting Ligands as usedherein include, but are not limited to: 1. Finnin, M. S. et al.Structures of Histone Deacetylase Homologue Bound to the TSA and SAHAInhibitors. Nature 40, 188-193 (1999).

(Derivatized where “R” designates a site for attachment, for example, ofa Linker group L or a —(L-DEGRON) group); and 2. Compounds as defined byformula (I) of PCT WO0222577 (“DEACETYLASE INHIBITORS”) (Derivatizedwhere a Linker group L or a —(L-DEGRON) group is attached, for example,via the hydroxyl group); CC. Human Lysine Methyltransferase dTAGTargeting Ligands: Human Lysine Methyltransferase dTAG Targeting Ligandsas used herein include, but are not limited to: 1. Chang et al.Structural Basis for G9a-Like protein Lysine MethyltransferaseInhibition by BIX- 1294. Nat. Struct. Biol. (2009) 16(3) 312.

(Derivatized where “R” designates a site for attachment, for example, ofa Linker group L or a —(L-DEGRON) group); 2. Liu, F. et al Discovery ofa 2,4-Diamino-7-aminoalkoxyquinazoline as a Potent and SelectiveInhibitor of Histone Methyltransferase G9a. J. Med. Chem. (2009) 52(24)7950

(Derivatized where “R” designates a potential site for attachment, forexample, of a Linker group L or a —(L-DEGRON) group); 3. Azacitidine(derivatized) (4-amino-1-(3-D-ribofuranosyl-1,3,5-triazin-2(1H)-one)(Derivatized where a Linker group L or a —(L-DEGRON) group is attached,for example, via the hydroxy or amino groups); and 4. Decitabine(derivatized)(4-amino-1-(2-deoxy-b-D-erythro-pentofuranosyl)-1,3,5-triazin-2(1H)-one) (Derivatized where a Linker group L or a —(L-DEGRON) group isattached, for example, via either of the hydroxy groups or at the aminogroup). DD. dTAG targeting ligands organized by functionalityAngiogenesis Inhibitors: Angiogenesis inhibitors include, but are notlimited to: 1. GA-1 (derivatized) and derivatives and analogs thereof,having the structure(s) and binding to Linkers as described in Sakamoto,et al., Development of Protacs to target cancer-promoting proteins forubiquitination and degradation, Mol Cell Proteomics 2003 December;2(12):1350-8; 2. Estradiol (derivatized), which may be bound to a Linkergroup L or a —(L-DEGRON) group as is generally described inRodriguez-Gonzalez, et al., Targeting steroid hormone receptors forubiquitination and degradation in breast and prostate cancer, Oncogene(2008) 27, 7201-7211; 3. Estradiol, testosterone (derivatized) andrelated derivatives, including but not limited to DHT and derivativesand analogs thereof, having the structure(s) and binding to a Linkergroup L or a —(L-DEGRON) group as generally described in Sakamoto, etal., Development of Protacs to target cancer-promoting proteins forubiquitination and degradation, Mol Cell Proteomics 2003 December;2(12):1350-8; and 4. Ovalicin, fumagillin (derivatized), and derivativesand analogs thereof, having the structure(s) and binding to a Linkergroup L or a —(L-DEGRON) group as is generally described in Sakamoto, etal., Protacs: chimeric molecules that target proteins to theSkpl-Cullin-F box complex for ubiquitination and degradation Proc NatlAcad Sci USA. 2001 Jul. 17; 98(15):8554-9 and U.S. Pat. No. 7,208,157.Immunosuppressive Compounds: Immunosuppressive compounds include, butare not limited to: 1. AP21998 (derivatized), having the structure(s)and binding to a Linker group L or a —(L- DEGRON) group as is generallydescribed in Schneekloth, et al., Chemical Genetic Control of ProteinLevels: Selective in Vivo Targeted Degradation, J. AM. CHEM. SOC. 2004,126, 3748- 3754; 2. Glucocorticoids (e.g., hydrocortisone, prednisone,prednisolone, and methylprednisolone) (Derivatized where a Linker groupL or a —(L-DEGRON) group is to bound, e.g. to any of the hydroxyls) andbeclometasone dipropionate (Derivatized where a Linker group or a —(L-DEGRON) is bound, e.g. to a proprionate); 3. Methotrexate (Derivatizedwhere a Linker group or a —(L-DEGRON) group can be bound, e.g. to eitherof the terminal hydroxyls); 4. Ciclosporin (Derivatized where a Linkergroup or a —(L-DEGRON) group can be bound, e.g. at any of the butylgroups); 5. Tacrolimus (FK-506) and rapamycin (Derivatized where aLinker group L or a —(L-DEGRON) group can be bound, e.g. at one of themethoxy groups); and 6. Actinomycins (Derivatized where a Linker group Lor a —(L-DEGRON) group can be bound, e.g. at one of the isopropylgroups). EE. Aryl Hydrocarbon Receptor (AHR) dTAG Targeting Ligands: AHRdTAG Targeting Ligands as used herein include, but are not limitedto: 1. Apigenin (Derivatized in a way which binds to a Linker group L ora —(L-DEGRON) group as is generally illustrated in Lee, et al., TargetedDegradation of the Aryl Hydrocarbon Receptor by the PROTAC Approach: AUseful Chemical Genetic Tool, Chem Bio Chem Volume 8, Issue 17, pages2058-2062, Nov. 23, 2007); and 2. SR1 and LGC006 (derivatized such thata Linker group L or a —(L-DEGRON) is bound), as described in Boitano, etal., Aryl Hydrocarbon Receptor Antagonists Promote the Expansion ofHuman Hematopoietic Stem Cells, Science 10 Sep. 2010: Vol. 329 no. 5997pp. 1345-1348. FF. RAF dTAG Targeting Ligands: RAF dTAG TargetingLigands as used herein include, but are not limited to:

PLX4032 (Derivatized where “R” designates a site for Linker group L or—(L-DEGRON) group attachment, for example). GG. FKBP dTAG TargetingLigands: FKBP dTAG Targeting Ligands as used herein include, but are notlimited to:

(Derivatized where “R” designates a site for a Linker group L or a—(L-DEGRON) group attachment, for example). HH. Androgen Receptor (AR)dTAG Targeting Ligands: AR dTAG Targeting Ligands as used hereininclude, but are not limited to: 1. RU59063 Ligand (derivatized) ofAndrogen Receptor

(Derivatized where “R” designates a site for a Linker group L or a—(L-DEGRON) group attachment, for example). 2. SARM Ligand (derivatized)of Androgen Receptor

(Derivatized where “R” designates a site for a Linker group L or a—(L-DEGRON) group attachment, for example). 3. Androgen Receptor LigandDHT (derivatized)

(Derivatized where “R” designates a site for a Linker group L or—(L-DEGRON) group attachment, for example). 4. MDV3100 Ligand(derivatized)

5. ARN-509 Ligand (derivatized)

6. Hexahydrobenzisoxazoles

7. Tetramethylcyclobutanes

II. Estrogen Receptor (ER) dTAG Targeting Ligands: ER dTAG TargetingLigands as used herein include, but are not limited to: 1. EstrogenReceptor Ligand

(Derivatized where “R” designates a site for Linker group L or—(L-DEGRON) group attachment). JJ. Thyroid Hormone Receptor (TR) dTAGTargeting Ligands: TR dTAG Targeting Ligands as used herein include, butare not limited to: 1. Thyroid Hormone Receptor Ligand (derivatized)

(Derivatized where “R” designates a site for Linker group L or—(L-DEGRON) group attachment and MOMO indicates a methoxymethoxy group).KK. HIV Protease dTAG Targeting Ligands: HIV Protease dTAG TargetingLigands as used herein include, but are not limited to: 1. Inhibitor ofHIV Protease (derivatized)

(Derivatized where “R” designates a site for Linker group L or—(L-DEGRON) group attachment). See, J. Med. Chem. 2010, 53, 521-538. 2.Inhibitor of HIV Protease

(Derivatized where “R” designates a potential site for Linker group L or—(L-DEGRON) group attachment). See, J. Med. Chem. 2010, 53, 521-538. LL.HIV Integrase dTAG Targeting Ligands: HIV Integrase dTAG TargetingLigands as used herein include, but are not limited to: 1. Inhibitor ofHIV Integrase (derivatized)

(Derivatized where “R” designates a site for Linker group L or—(L-DEGRON) group attachment). See, J. Med. Chem. 2010, 53, 6466. 2.Inhibitor of HIV Integrase (derivatized)

3. Inhibitor of HIV integrase (derivatized)

(Derivatized where “R” designates a site for Linker group L or—(L-DEGRON) group attachment). See, J. Med. Chem. 2010, 53, 6466. MM.HCV Protease dTAG Targeting Ligands: HCV Protease dTAG Targeting Ligandsas used herein include, but are not limited to: 1. Inhibitors of HCVProtease (Derivatized)

(Derivatized where “R” designates a site for Linker group L or—(L-DEGRON) group attachment). NN. Acyl-Protein Thioesterase-1 and -2(APT1 and APT2) dTAG Targeting Ligands: Acyl-Protein Thioesterase-1 and-2 (APT1 and APT2) dTAG Targeting Ligands as used herein include, butare not limited to: 1. Inhibitor of APT1 and APT2 (Derivatized)

(Derivatized where “R” designates a site for Linker group L or—(L-DEGRON) group attachment). See, Angew. Chem. Int. Ed. 2011, 50,9838-9842, where L is a Linker group as otherwise described herein andsaid Degron group is as otherwise described herein such that the Linkerbinds the Degron group to a dTAG Targeting Ligand group as otherwisedescribed herein. OO. BCL2 dTAG Targeting Ligands: BCL2 dTAG TargetingLigands as used herein include, but are not limited to:

wherein: R is the point at which the Linker is attached. PP. BCL-XL dTAGTargeting Ligands: BCL-XL dTAG Targeting Ligands as used herein include,but are not limited to:

wherein: R is the point at which the Linker is attached. QQ. FA BindingProtein dTAG Targeting Ligands: FA dTAG Targeting Ligands as used hereininclude, but are not limited to:

wherein: R is the point at which the Linker is attached. RR.FLAP-5-Lipoxygenase Activating Protein dTAG Targeting Ligands:FLAP-5-Lipoxygenase Activating Protein dTAG Targeting Ligands as usedherein include, but are not limited to:

wherein: R is the point at which the Linker is attached. SS. HDAC6 ZnFinger Domain dTAG Targeting Ligands: HDAC6 Zn Finger Domain dTAGTargeting Ligands as used herein include, but are not limited to:

wherein: R is the point at which the Linker is attached. TT. KringleDomain V 4BVV dTAG Targeting Ligands: Kringle Domain V 4BVV dTAGTargeting Ligands as used herein include, but are not limited to:

wherein: R is the point at which the Linker is attached. UU.Lactoylglutathione Lyase dTAG Targeting Ligands: LactoylglutathioneLyase dTAG Targeting Ligands as used herein include, but are not limitedto:

wherein: R is the point at which the Linker is attached. VV. mPGES-1dTAG Targeting Ligands: mPGES-1 dTAG Targeting Ligands as used hereininclude, but are not limited to:

wherein: R is the point at which the Linker is attached. WW. MTH1 dTAGTargeting Ligands: MTH1 dTAG Targeting Ligands as used herein include,but are not limited to:

wherein: R is the point at which the Linker is attached. XX. PARP14 dTAGTargeting Ligands: PARP14 dTAG Targeting Ligands as used herein include,but are not limited to:

wherein: R is the point at which the Linker is attached. YY. PARP15 dTAGTargeting Ligands: PARP15 dTAG Targeting Ligands as used herein include,but are not limited to:

wherein: R is the point at which the Linker is attached. ZZ. PDZ domaindTAG Targeting Ligands: PDZ domain dTAG Targeting Ligands as used hereininclude, but are not limited to:

wherein: R and R′ are points at which the Linker(s) are attached. AAA.PHIP dTAG Targeting Ligands: PHIP dTAG Targeting Ligands as used hereininclude, but are not limited to:

wherein: R is the point at which the Linker is attached. BBB.Phospholipase A2 domain dTAG Targeting Ligands: Phospholipase A2 domaindTAG Targeting Ligands as used herein include, but are not limited to:

wherein: R is the point at which the Linker is attached. CCC. ProteinS100-A7 2WOS dTAG Targeting Ligands: Protein S100-A7 2WOS dTAG TargetingLigands as used herein include, but are not limited to:

wherein: R is the point at which the Linker is attached. DDD. Saposin-BdTAG Targeting Ligands: Saposin-B dTAG Targeting Ligands as used hereininclude, but are not limited to:

wherein: R is the point at which the Linker is attached. EEE. Sec7 dTAGTargeting Ligands: Sec7 dTAG Targeting Ligands as used herein include,but are not limited to:

wherein: R is the point at which the Linker is attached. FFF. SH2 domainof pp60 Src dTAG Targeting Ligands: SH2 domain of pp60 Src dTAGTargeting Ligands as used herein include, but are not limited to:

wherein: R is the point at which the Linker is attached. GGG. Tank1 dTAGTargeting Ligands: Tank1 dTAG Targeting Ligands as used herein include,but are not limited to

wherein: R is the point at which the Linker is attached. HHH. Ubc9 SUMOE2 ligase SF6D dTAG Targeting Ligands: Ubc9 SUMO E2 ligase SF6D dTAGTargeting Ligands as used herein include, but are not limited to:

wherein: R is the point at which the Linker is attached. III. Src(c-Src) dTAG Targeting Ligands: Src dTAG Targeting Ligands as usedherein include, but are not limited to: 1. Src Targenting Ligandsincluding AP23464:

wherein: R is the point at which the Linker is attached. 2. Src-AS1and/or Src AS2 Targeting Ligands including:

wherein: R is the point at which the Linker is attached. JJJ. JAK3 dTAGTargeting Ligands: JAK3 dTAG Targeting Ligands as used herein include,but are not limited to: 1. Targeting Ligands that target JAK3, includingTofacitinib:

wherein: R is the point at which the Linker is attached. KKK. Abl dTAGTargeting Ligands: Abl dTAG Targeting Ligands as used herein include,but are not limited to: 1. Targeting Ligands that target Abl, includingTofacitinib and Ponatinib:

wherein: R is the point at which the Linker is attached. LLL. MEK1 dTAGTargeting Ligands: MEK1 dTAG Targeting Ligands as used herein include,but are not limited to: 1. Targeting Ligands that target MEK1, includingPD318088, Trametinib, and G-573:

wherein: R is the point at which the Linker is attached. MMM. KIT dTAGTargeting Ligands: KIT dTAG Targeting Ligands as used herein include,but are not limited to: 1. Targeting Ligands that target KIT, includingRegorafenib:

wherein: R is the point at which the Linker is attached. NNN. HIVReverse Transcriptase dTAG Targeting Ligands: HIV Reverse TranscriptasedTAG Targeting Ligands as used herein include, but are not limitedto: 1. Targeting Ligands that target HIV Reverse Transcriptase,including Efavirenz, Tenofovir, Emtricitabine, Ritonavir, Raltegravir,and Atazanavir:

wherein: R is the point at which the Linker is attached. OOO. HIVProtease dTAG Targeting Ligands: HIV Protease dTAG Targeting Ligands asused herein include, but are not limited to: 1. Targeting Ligands thattarget HIV Protease, including Ritonavir, Raltegravir, and Atazanavir:

wherein: R is the point at which the Linker is attached.

In one embodiment any of the above dTAG Targeting Ligands of Table T isused to target any dTAG described herein.

Many dTag targeting ligands are capable of binding to more than onedTAG, for example. Afatinib binds to the EGFR, the ErbB2, and the ErbB4protein This allows for dual attack of many proteins, as exemplified inTable Z. In one embodiment, the dTAG targeting ligand is selected fromthe “dTAG targeting ligand” column in Table Z and the dTAG is selectedfrom the corresponding “dTAG” row.

TABLE Z dTAG Targeting Ligands and corresponding dTAG dTAG TargetingLigand dTAG Afatinib EGFR, ErbB2/4

Axitinib  

VEGFR1/2/3, PDGFRβ, Kit Bosutinib  

BCR-Abl, Src, Lyn, Hck

Cabozantinib  

RET, c-Met, VEGFR1/2/3, Kit, TrkB, Flt3, Axl, Tie2

Ceritinib  

ALK, IGF-1R, InsR, ROS1

Crizotinib  

ALK, c-Met (HGFR), ROS1, MST1R

Dabrafenib B-Raf

Dasatinib  

BCR-Abl, Src, Lck, Lyn, Yes, Fyn, Kit, EphA2, PDGFRβ

Erlotinib EGFR

Everolimus  

HER2— breast cancer, PNET, RCC, RAML, SEGA

Gefitinib EGFR, PDGFR

Ibrutinib BTK

Imatinib  

BCR-Abl, Kit, PDGFR

Lapatinib EGFR, ErbB2

Lenvatinib  

VEGFR1/2/3, FGFR1/2/3/4, PDGFRα, Kit, RET

Nilotinib  

BCR-Abl, PDGFR, DDR1

Nintedanib  

FGFR1/2/3, Flt3, Lck, PDGFRα/β, VEGFR1/2/3

Palbociclib CDK4/6

Pazopanib  

VEGFR1/2/3, PDGFRα/β, FGFR1/3, Kit, Lck, Fms, Itk

Ponatinib  

BCR-Abl, BCR- Abl T315I, VEGFR, PDGFR, FGFR, EphR, Src family kinases,Kit, RET, Tie2, Flt3 Regorafenib  

VEGFR1/2/3, BCR-Abl, B- Raf, B-Raf (V600E), Kit, PDGFRα/β, RET, FGFR1/2,Tie2, and Eph2A

Ruxolitinib JAK1/2

Sirolimus FKBP12/mTOR

Sorafenib  

B-Raf, CDK8, Kit, Flt3, RET, VEGFR1/2/3, PDGFR

Sunitinib  

PDGFRα/β, VEGFR1/2/3, Kit, Flt3, CSF-1R, RET

Temsirolimus FKBP12/mTOR

Tofacitinib JAK3

Trametinib  

MEK1/2

Vandetanib  

EGFR, VEGFR, RET, Tie2, Brk, EphR

Vemurafenib  

A/B/C-Raf and B-Raf (V600E)

In certain embodiments, the present application includes compoundscontaining the dTAG Targeting Ligands shown in Table 1.

TABLE 1 dTAG Targeting Ligands 1-6 Compound Structure TL1

Ang. Chem. Intl Ed. 50, 9378 (2011) TL2

TL5

JACS 115, 9925 (1993) TL7

TL3

TL4

TL6

In certain embodiments, the dTAG Targeting Ligand is a compound ofFormula TL-I:

or a pharmaceutically acceptable salt thereof, wherein:

A¹ is S or C═C;

A² is NRa⁵ or O;

nn1 is 0, 1, or 2;

each Ra¹ is independently C₁-C₃ alkyl, (CH₂)₀₋₃—CN, (CH₂)₀₋₃-halogen,(CH₂)₀₋₃—OH, (CH₂)₀₋₃—C₁-C₃ alkoxy, C(O)NRa⁵L, OL, NRa⁵L, or L;

Ra² is H, C₁-C₆ alkyl, (CH₂)₀₋₃-heterocyclyl, (CH₂)₀₋₃-phenyl, or L,wherein the heterocyclyl comprises one saturated 5- or 6-membered ringand 1-2 heteroatoms selected from N, O, and S and is optionallysubstituted with C₁-C₃ alkyl, L, or C(O)L, and wherein the phenyl isoptionally substituted with C₁-C₃ alkyl, CN, halogen, OH, C₁-C₃ alkoxy,or L;

nn2 is 0, 1, 2, or 3;

each Ra³ is independently C₁-C₃ alkyl, (CH₂)₀₋₃—CN, (CH₂)₀₋₃-halogen, L,or C(O)NRa⁵L;

Ra⁴ is C₁-C₃ alkyl;

Ra⁵ is H or C₁-C₃ alkyl; and

L is a Linker,

provided that the compound of Formula TL-I is substituted with only oneL.

In certain embodiments, is

In certain embodiments, is

In certain embodiments, A¹ is S.

In certain embodiments, A¹ is C═C.

In certain embodiments, A² is NRa⁵. In further embodiments, Ra⁵ is H. Inother embodiments, Ra⁵ is C₁-C₃ alkyl (e.g., methyl, ethyl, propyl, ori-propyl). In further embodiments, Ra⁵ is methyl.

In certain embodiments, A² is O.

In certain embodiments, nn1 is 0.

In certain embodiments, nn1 is 1.

In certain embodiments, nn1 is 2.

In certain embodiments, at least one Ra¹ is C₁-C₃ alkyl (e.g., methyl,ethyl, propyl, or i-propyl). In further embodiments, at least one Ra¹ ismethyl. In further embodiments, two Ra¹ are methyl.

In certain embodiments, at least one Ra¹ is CN, (CH₂)—CN, (CH₂)₂—CN, or(CH₂)₃—CN. In further embodiments, at least one Ra¹ is (CH₂)—CN.

In certain embodiments, at least one Ra¹ is halogen (e.g., F, Cl, orBr), (CH₂)-halogen, (CH₂)₂-halogen, or (CH₂)₃-halogen. In furtherembodiments, at least one Ra¹ is C₁, (CH₂)—Cl, (CH₂)₂—Cl, or (CH₂)₃—Cl.

In certain embodiments, at least one Ra¹ is OH, (CH₂)—OH, (CH₂)₂—OH, or(CH₂)₃—OH.

In certain embodiments, at least one Ra¹ is C₁-C₃ alkoxy (e.g., methoxy,ethoxy, or propoxy), (CH₂)—C₁-C₃ alkoxy, (CH₂)₂—C₁-C₃ alkoxy, or(CH₂)₃—C₁-C₃ alkoxy. In certain embodiments, at least one Ra^(t) ismethoxy.

In certain embodiments, one Ra¹ is C(O)NRa⁵L. In further embodiments,Ra⁵ is H. In other embodiments, Ra⁵ is C₁-C₃ alkyl (e.g., methyl, ethyl,propyl, or i-propyl).

In certain embodiments, one Ra¹ is OL.

In certain embodiments, one Ra¹ is NRa⁵L. In further embodiments, Ra⁵ isH. In other embodiments, Ra⁵ is C₁-C₃ alkyl (e.g., methyl, ethyl,propyl, or i-propyl). In other embodiments, Ra⁵ is methyl.

In certain embodiments, one Ra¹ is L.

In certain embodiments, Ra² is H.

In certain embodiments, Ra² is straight-chain C₁-C₆ or branched C₃-C₆alkyl (e.g., methyl, ethyl, propyl, i-propyl, butyl, i-butyl, t-butyl,pentyl, or hexyl). In further embodiments, Ra² is methyl, ethyl, ort-butyl.

In certain embodiments, Ra² is heterocyclyl, (CH₂)-heterocyclyl,(CH₂)₂-heterocyclyl, or (CH₂)₃-heterocyclyl. In further embodiments, Ra²is (CH₂)₃-heterocyclyl. In further embodiments, the heterocyclyl isselected from pyrrolidinyl, pyrazolidinyl, imidazolidinyl, oxazolidinyl,isoxazolidinyl, thiazolidinyl, isothiazolidinyl, piperidinyl,piperazinyl, hexahydropyrimidinyl, morpholinyl, and thiomorpholinyl. Infurther embodiments, the heterocyclyl is piperazinyl.

In certain embodiments, the heterocyclyl is substituted with C₁-C₃ alkyl(e.g., methyl, ethyl, propyl, or i-propyl).

In certain embodiments, the heterocyclyl is substituted with C(O)L.

In certain embodiments, the heterocyclyl is substituted with L.

In certain embodiments, Ra² is phenyl, (CH₂)-phenyl, (CH₂)₂-phenyl, or(CH₂)₃-phenyl. In further embodiments, Ra² is phenyl.

In certain embodiments, the phenyl is substituted with C₁-C₃ alkyl(e.g., methyl, ethyl, propyl, or i-propyl). In certain embodiments, thephenyl is substituted with CN. In certain embodiments, the phenyl issubstituted with halogen (e.g., F, Cl, or Br). In certain embodiments,the phenyl is substituted with OH. In certain embodiments, the phenyl issubstituted with C₁-C₃ alkoxy (e.g., methoxy, ethoxy, or propoxy).

In certain embodiments, the phenyl is substituted with L.

In certain embodiments, Ra² is L.

In certain embodiments, nn2 is 0.

In certain embodiments, nn2 is 1.

In certain embodiments, nn2 is 2.

In certain embodiments, nn2 is 3.

In certain embodiments, at least one Ra³ is C₁-C₃ alkyl (e.g., methyl,ethyl, propyl, or i-propyl). In further embodiments, at least one Ra³ ismethyl.

In certain embodiments, at least one Ra³ is CN, (CH₂)—CN, (CH₂)₂—CN, or(CH₂)₃—CN. In further embodiments, at least one Ra³ is CN.

In certain embodiments, at least one Ra³ is halogen (e.g., F, Cl, orBr), (CH₂)-halogen, (CH₂)₂-halogen, or (CH₂)₃-halogen. In furtherembodiments, at least one Ra³ is C₁, (CH₂)—Cl, (CH₂)₂—Cl, or (CH₂)₃—Cl.In further embodiments, at least one Ra³ is C₁.

In certain embodiments, one Ra³ is L.

In certain embodiments, one Ra³ is C(O)NRa⁵L. In further embodiments,Ra⁵ is H. In other embodiments, Ra⁵ is C₁-C₃ alkyl (e.g., methyl, ethyl,propyl, or i-propyl).

In certain embodiments, Ra⁴ is C₁-C₃ alkyl (e.g., methyl, ethyl, propyl,or i-propyl). In further embodiments, Ra⁴ is methyl.

In certain embodiments, Ra⁵ is H.

In certain embodiments, Ra⁵ is C₁-C₃ alkyl (e.g., methyl, ethyl, propyl,or i-propyl). In further embodiments, Ra⁵ is methyl.

In certain embodiments,

and A¹ is S.

In certain embodiments,

and A¹ is C═C.

In certain embodiments,

and A¹ is C═C.

In certain embodiments, A² is NH, and Ra² is (CH₂)₀₋₃-heterocyclyl. Infurther embodiments, Ra² is (CH₂)₃-heterocyclyl. In further embodiments,the heterocyclyl is piperazinyl. In further embodiments, theheterocyclyl is substituted with C₁-C₃ alkyl, L, or C(O)L.

In certain embodiments, A² is NH, and Ra² is (CH₂)₀₋₃-phenyl. In furtherembodiments, Ra² is phenyl. In further embodiments, the phenyl issubstituted with OH or L.

In certain embodiments, A² is NH, and Ra² is L.

In certain embodiments, A² is NH, and Ra² is H or C₁-C₆ alkyl. Infurther embodiments, Ra² is C₁-C₄ alkyl.

In certain embodiments, A² is O, and Ra² is H or C₁-C₆ alkyl. In furtherembodiments, Ra² is C₁-C₄ alkyl.

In certain embodiments, a dTAG Targeting Ligand is a compound of FormulaTL-I1:

or a pharmaceutically acceptable salt thereof, wherein A², Ra¹, Ra²,Ra³, Ra⁴, Ra⁵, nn1, and nn2 are each as defined above in Formula TL-I.

Each of A², Ra¹, Ra², Ra³, Ra⁴, Ra⁵, nn1, and nn2 may be selected fromthe moieties described above in Formula TL-I. Each of the moietiesdefined for one of A², Ra¹, Ra², Ra³, Ra⁴, Ra⁵, nn1, and nn2, can becombined with any of the moieties defined for the others of A², Ra¹,Ra², Ra³, Ra⁴, Ra⁵, nn1, and nn2, as described above in Formula TL-I.

In certain embodiments, a dTAG Targeting Ligand is a compound of FormulaTL-I1a-TL-I1d:

or a pharmaceutically acceptable salt thereof, wherein:

each Ra⁶ is independently C₁-C₃ alkyl, (CH₂)₀₋₃—CN, (CH₂)₀₋₃-halogen,(CH₂)₀₋₃—OH, or (CH₂)₀₋₃—C₁-C₃ alkoxy;

Ra⁷ is (CH₂)₀₋₃-heterocyclyl, (CH₂)₀₋₃-phenyl, or L, wherein theheterocyclyl comprises one saturated 5- or 6-membered ring and 1-2heteroatoms selected from N, O, and S and is substituted with L orC(O)L, and wherein the phenyl is substituted with L;

Ra⁸ is H, C₁-C₆ alkyl, (CH₂)₀₋₃-heterocyclyl, or (CH₂)₀₋₃-phenyl,wherein the heterocyclyl comprises one saturated 5- or 6-membered ringand 1-2 heteroatoms selected from N, O, and S and is optionallysubstituted with C₁-C₃ alkyl, and wherein the phenyl is optionallysubstituted with C₁-C₃ alkyl, CN, halogen, OH, or C₁-C₃ alkoxy;

Ra¹⁰ is C₁-C₃ alkyl, (CH₂)₀₋₃—CN, or (CH₂)₀₋₃-halogen; and

A², Ra⁴, Ra⁵, nn1, and L are each as defined above in Formula TL-I.

In certain embodiments, nn1 is 0.

In certain embodiments, nn1 is 1.

In certain embodiments, nn1 is 2.

In certain embodiments, at least one Ra⁶ is C₁-C₃ alkyl (e.g., methyl,ethyl, propyl, or i-propyl). In further embodiments, at least one Ra⁶ ismethyl. In further embodiments, two Ra⁶ are methyl.

In certain embodiments, at least one Ra⁶ is CN, (CH₂)—CN, (CH₂)₂—CN, or(CH₂)₃—CN. In further embodiments, at least one Ra⁶ is (CH₂)—CN.

In certain embodiments, at least one Ra⁶ is halogen (e.g., F, Cl, orBr), (CH₂)-halogen, (CH₂)₂-halogen, or (CH₂)₃-halogen. In furtherembodiments, at least one Ra⁶ is C₁, (CH₂)—Cl, (CH₂)₂—Cl, or (CH₂)₃—Cl.

In certain embodiments, at least one Ra⁶ is OH, (CH₂)—OH, (CH₂)₂—OH, or(CH₂)₃—OH.

In certain embodiments, at least one Ra⁶ is C₁-C₃ alkoxy (e.g., methoxy,ethoxy, or propoxy), (CH₂)—C₁-C₃ alkoxy, (CH₂)₂—C₁-C₃ alkoxy, or(CH₂)₃—C₁-C₃ alkoxy. In certain embodiments, at least one Ra⁶ ismethoxy.

In certain embodiments, Ra⁷ is heterocyclyl, (CH₂)-heterocyclyl,(CH₂)₂-heterocyclyl, or (CH₂)₃-heterocyclyl. In further embodiments, Ra⁷is (CH₂)₃-heterocyclyl. In further embodiments, the heterocyclyl isselected from pyrrolidinyl, pyrazolidinyl, imidazolidinyl, oxazolidinyl,isoxazolidinyl, thiazolidinyl, isothiazolidinyl, piperidinyl,piperazinyl, hexahydropyrimidinyl, morpholinyl, and thiomorpholinyl. Infurther embodiments, the heterocyclyl is piperazinyl.

In certain embodiments, the heterocyclyl is substituted with C(O)L.

In certain embodiments, the heterocyclyl is substituted with L.

In certain embodiments, Ra⁷ is phenyl, (CH₂)-phenyl, (CH₂)₂-phenyl, or(CH₂)₃-phenyl. In further embodiments, Ra⁷ is phenyl.

In certain embodiments, Ra⁷ is L.

In certain embodiments, Ra⁸ is H.

In certain embodiments, Ra⁸ is straight-chain C₁-C₆ or branched C₃-C₆alkyl (e.g., methyl, ethyl, propyl, i-propyl, butyl, i-butyl, t-butyl,pentyl, or hexyl). In further embodiments, Ra⁸ is methyl, ethyl, ort-butyl.

In certain embodiments, Ra⁸ is heterocyclyl, (CH₂)-heterocyclyl,(CH₂)₂-heterocyclyl, or (CH₂)₃-heterocyclyl. In further embodiments, Ra⁸is (CH₂)₃-heterocyclyl. In further embodiments, the heterocyclyl isselected from pyrrolidinyl, pyrazolidinyl, imidazolidinyl, oxazolidinyl,isoxazolidinyl, thiazolidinyl, isothiazolidinyl, piperidinyl,piperazinyl, hexahydropyrimidinyl, morpholinyl, and thiomorpholinyl. Infurther embodiments, the heterocyclyl is piperazinyl.

In certain embodiments, the heterocyclyl is substituted with C₁-C₃ alkyl(e.g., methyl, ethyl, propyl, or i-propyl).

In certain embodiments, Ra⁸ is phenyl, (CH₂)-phenyl, (CH₂)₂-phenyl, or(CH₂)₃-phenyl. In further embodiments, Ra⁸ is phenyl.

In certain embodiments, the phenyl is substituted with C₁-C₃ alkyl(e.g., methyl, ethyl, propyl, or i-propyl). In certain embodiments, thephenyl is substituted with CN. In certain embodiments, the phenyl issubstituted with halogen (e.g., F, Cl, or Br). In certain embodiments,the phenyl is substituted with OH. In certain embodiments, the phenyl issubstituted with C₁-C₃ alkoxy (e.g., methoxy, ethoxy, or propoxy).

In certain embodiments, Ra¹⁰ is C₁-C₃ alkyl (e.g., methyl, ethyl,propyl, or i-propyl).

In certain embodiments, Ra¹⁰ is CN, (CH₂)—CN, (CH₂)₂—CN, or (CH₂)₃—CN.

In certain embodiments, Ra¹ is halogen (e.g., F, Cl, or Br),(CH₂)-halogen, (CH₂)₂-halogen, or (CH₂)₃-halogen. In furtherembodiments, Ra¹ is C₁, (CH₂)—Cl, (CH₂)₂—Cl, or (CH₂)₃—Cl. In furtherembodiments, Ra¹⁰ is C₁.

Each of A², Ra⁴, Ra⁵, and nn1 may be selected from the moietiesdescribed above in Formula TL-I. Each of the moieties defined for one ofA², Ra⁴, Ra⁵, Ra⁶, Ra⁷, Ra⁸, Ra¹⁰, and nn1, can be combined with any ofthe moieties defined for the others of A², Ra⁴, Ra⁵, Ra⁶, Ra⁷, Ra⁸,Ra¹⁰, and nn1, as described above and in Formula TL-I.

In certain embodiments, a dTAG Targeting Ligand is a compound of FormulaTL-I2:

or a pharmaceutically acceptable salt thereof, wherein A², Ra¹, Ra²,Ra³, Ra⁴, Ra⁵, nn1, and nn2 are each as defined above in Formula TL-I.

Each of A², Ra¹, Ra², Ra³, Ra⁴, Ra⁵, nn1, and nn2 may be selected fromthe moieties described above in Formula TL-I. Each of the moietiesdefined for one of A², Ra¹, Ra², Ra³, Ra⁴, Ra⁵, nn1, and nn2, can becombined with any of the moieties defined for the others of A², Ra¹,Ra², Ra³, Ra⁴, Ra⁵, nn1, and nn2, as described above in Formula TL-I.

In certain embodiments, a dTAG Targeting Ligand is a compound of FormulaTL-I2a-TL-I2c:

or a pharmaceutically acceptable salt thereof, wherein A², Ra⁴, Ra⁵,nn1, and L are each as defined above in Formula TL-I, and Ra⁶, Ra⁷, Ra⁸,and Ra¹⁰ are each as defined above in Formula TL-I1a-TL-I1d.

Each of A², Ra⁴, Ra⁵, and nn1 may be selected from the moietiesdescribed above in Formula TL-I, and each of Ra⁶, Ra⁷, Ra⁸, and Ra¹⁰ maybe selected from the moieties described above in Formula TL-I1a-TL-I1d.Each of the moieties defined for one of A², Ra⁴, Ra⁵, Ra⁶, Ra⁷, Ra⁸,Ra¹⁰, and nn1, can be combined with any of the moieties defined for theothers of A², Ra⁴, Ra⁵, Ra⁶, Ra⁷, Ra⁸, Ra¹⁰, and nn1, as described abovein Formula TL-I and TL-I1a-TL-I1d.

In certain embodiments, a dTAG Targeting Ligand is a compound of FormulaTL-I3:

or a pharmaceutically acceptable salt thereof.

A², Ra¹, Ra², Ra³, Ra⁴, Ra⁵, nn1, and nn2 are each as defined above inFormula TL-I. Each of A², Ra¹, Ra², Ra³, Ra⁴, Ra⁵, nn1, and nn2 may beselected from the moieties described above in Formula TL-I. Each of themoieties defined for one of A², Rat, Ra², Ra³, Ra⁴, Ra⁵, nn1, and nn2,can be combined with any of the moieties defined for the others of A²,Ra¹, Ra², Ra³, Ra⁴, Ra⁵, nn1, and nn2, as described above in FormulaTL-I.

In certain embodiments, a dTAG Targeting Ligand is a compound of FormulaTL-I3a-TL-I3c:

or a pharmaceutically acceptable salt thereof, wherein:

Ra⁹ is C(O)NRa⁵L, OL, NRa⁵L, or L;

A², Ra⁴, Ra⁵, nn1, and L are each as defined above in Formula TL-I; and

Ra⁶, Ra⁷, Ra⁸, and Ra¹o are each as defined above in FormulaTL-I1a-TL-I1d.

In certain embodiments, Ra⁹ is C(O)NRa⁵L. In further embodiments, Ra⁵ isH. In other embodiments, Ra⁵ is C₁-C₃ alkyl (e.g., methyl, ethyl,propyl, or i-propyl).

In certain embodiments, Ra⁹ is OL.

In certain embodiments, Ra⁹ is NRa⁵L. In further embodiments, Ra⁵ is H.In other embodiments, Ra⁵ is C₁-C₃ alkyl (e.g., methyl, ethyl, propyl,or i-propyl). In other embodiments, Ra₅ is methyl.

In certain embodiments, Ra⁹ is L.

Each of A², Ra⁴, Ra⁵, and nn1 may be selected from the moietiesdescribed above in Formula TL-I, and each of Ra⁶, Ra⁷, Ra⁸, and Ra¹o maybe selected from the moieties described above in Formula TL-I1a-TL-I1d.Each of the moieties defined for one of A², Ra⁴, Ra⁵, Ra⁶, Ra⁷, Ra⁸,Ra⁹, Ra¹o, and nn1, can be combined with any of the moieties defined forthe others of A², Ra⁴, Ra⁵, Ra⁶, Ra⁷, Ra⁸, Ra⁹, Ra¹⁰, and nn1, asdescribed above and in Formula TL-I and TL-I1a-TL-I1d.

In certain embodiments, a dTAG Targeting Ligand is a compound of FormulaTL-VI:

or a pharmaceutically acceptable salt thereof, wherein:

Rf¹ is C(O)NRf²L, OL, NRf²L, or L;

Rf² is independently H or C₁-C₃ alkyl; and

L is a Linker.

In certain embodiments, Rf¹ is C(O)NRf²L. In further embodiments, Rf² isH. In other embodiments, Rf² is C₁-C₃ alkyl (e.g., methyl, ethyl,propyl, or i-propyl).

In certain embodiments, Rf¹ is OL.

In certain embodiments, Rf¹ is NRe⁴L. In further embodiments, Rf² is H.In other embodiments, Rf² is C₁-C₃ alkyl (e.g., methyl, ethyl, propyl,or i-propyl). In other embodiments, Rf² is methyl.

In certain embodiments, Rf¹ is L.

In certain embodiments, a dTAG Targeting Ligand is a compound of FormulaTL-VII:

or a pharmaceutically acceptable salt thereof, wherein:

T⁷ is CH₂ or CH₂CH₂;

Rg¹ is C(O)Rg⁵ or (CH₂)₁₋₃Rg⁶;

nn10 is 0, 1, 2, or 3;

nn11 is 0, 1, 2, or 3;

each Rg² is independently C₁-C₃ alkyl, C₁-C₃ alkoxy, CN, or halogen; Rg³is C(O)NRg⁴L, OL, NRg⁴L, L, O—(CH₂)₁₋₃—C(O)NRg⁴L, orNHC(O)—(CH₂)₁₋₃—C(O)NRg⁴L;

Rg⁴ is H or C₁-C₃ alkyl;

Rg⁵ is C₁-C₆ alkyl;

Rg⁶ is phenyl optionally substituted with C₁-C₃ alkyl, C₁-C₃ alkoxy, CN,or halogen; and

L is a Linker.

In certain embodiments, T⁷ is CH₂.

In certain embodiments, T⁷ is CH₂CH₂.

In certain embodiments, Rg¹ is C(O)Rg⁵.

In certain embodiments, Rg¹ is (CH₂)—Rg⁶, (CH₂)₂—Rg⁶, or (CH₂)₃—Rg⁶.

In certain embodiments, Rg⁵ is straight-chain C₁-C₆ or branched C₃-C₆alkyl (e.g., methyl, ethyl, propyl, i-propyl, butyl, i-butyl, t-butyl,pentyl, or hexyl).

In certain embodiments, Rg⁶ is unsubstituted phenyl.

In certain embodiments, Rg⁶ is phenyl substituted with one, two, three,or more substituents independently selected from C₁-C₃ alkyl (e.g.,methyl, ethyl, propyl, or i-propyl), C₁-C₃ alkoxy (e.g., methoxy,ethoxy, or propoxy), CN, and halogen (e.g., F, Cl, or Br).

In certain embodiments, nn10 is 0.

In certain embodiments, nn10 is 1.

In certain embodiments, nn10 is 2.

In certain embodiments, nn10 is 3.

In certain embodiments, nn11 is 0.

In certain embodiments, nn11 is 1.

In certain embodiments, nn11 is 2.

In certain embodiments, nn11 is 3.

In certain embodiments, at least one Rg² is C₁-C₃ alkyl (e.g., methyl,ethyl, propyl, or i-propyl). In further embodiments, at least one Rg² ismethyl.

In certain embodiments, at least one Rg² is C₁-C₃ alkoxy (e.g., methoxy,ethoxy, or propoxy). In further embodiments, at least one Rg² ismethoxy.

In certain embodiments, at least one Rg² is CN.

In certain embodiments, at least one Rg² is halogen (e.g., F, Cl, orBr).

In certain embodiments, Rg³ is C(O)NRg⁴L. In further embodiments, Rg⁴ isH. In other embodiments, Rg⁴ is C₁-C₃ alkyl (e.g., methyl, ethyl,propyl, or i-propyl).

In certain embodiments, Rg³ is OL.

In certain embodiments, Rg³ is NRg⁴L. In further embodiments, Rg⁴ is H.In other embodiments, Rg⁴ is C₁-C₃ alkyl (e.g., methyl, ethyl, propyl,or i-propyl). In other embodiments, Rg⁴ is methyl.

In certain embodiments, Rg³ is L.

In certain embodiments, Rg³ is O—(CH₂)—C(O)NRg⁴L, O—(CH₂)₂—C(O)NRg⁴L, orO—(CH₂)₃—C(O)NRg⁴L. In further embodiments, Rg³ is O—(CH₂)—C(O)NRg⁴L. Infurther embodiments, Rg⁴ is H. In other embodiments, Rg⁴ is C₁-C₃ alkyl(e.g., methyl, ethyl, propyl, or i-propyl).

In certain embodiments, Rg³ is NHC(O)—(CH₂)—C(O)NRg⁴L,NHC(O)—(CH₂)₂—C(O)NRg⁴L, or NHC(O)—(CH₂)₃—C(O)NRg⁴L. In furtherembodiments, Rg³ is NHC(O)—(CH₂)—C(O)NRg⁴L, NHC(O)—(CH₂)₂—C(O)NRg⁴L. Infurther embodiments, Rg³ is NHC(O)—(CH₂)₂—C(O)NRg⁴L. In furtherembodiments, Rg⁴ is H. In other embodiments, Rg⁴ is C₁-C₃ alkyl (e.g.,methyl, ethyl, propyl, or i-propyl).

In certain embodiments, the dTAG Targeting Ligand is selected from thestructures of FIG. 28, wherein R is the point at which the Linker isattached.

In certain embodiments, the dTAG Targeting Ligands or targets are chosenbased on existence (known dTAG binding moieties) and ability to developpotent and selective ligands with functional positions that canaccommodate a Linker. Some embodiments relate to dTAG Targeting Ligandswith less selectivity, which may benefit from degradation coupled withproteomics as a measure of compound selectivity or target ID.

Some embodiments of the present application relate to degradation orloss of 30% to 100% of the CAR. Certain embodiments relate to the lossof 50-100% of the CAR. Other embodiments relate to the loss of 75-95% ofthe CAR.

Non-limiting examples of heterobifunctional compounds for use in thepresent invention include those of FIGS. 29, 30, 31, and 32.

FIG. 29, provides specific heterobifunctional compounds for use in thepresent invention.

FIG. 30, provides specific heterobifunctional compounds for use in thepresent invention, wherein X in the above structures is a halogen chosenfrom F, Cl, Br, and I.

FIG. 31, provides specific heterobifunctional compounds for use in thepresent invention.

FIG. 32, provides heterobifunctional compounds for use in the presentinvention,

wherein:

R^(AR1) is selected from:

and

R^(AR2) is selected from:

and

Additional compounds for use in the present invention include thestructures of FIG. 33.

Some of the foregoing heterobifunctional compounds include one or moreasymmetric centers, and thus can exist in various isomeric forms, e.g.,stereoisomers and/or diastereomers. Thus, compounds and pharmaceuticalcompositions thereof may be in the form of an individual enantiomer,diastereomer, or geometric isomer, or may be in the form of a mixture ofstereoisomers. In certain embodiments, the compounds of the applicationare enantiopure compounds. In certain other embodiments, mixtures ofstereoisomers or diastereomers are provided.

Furthermore, certain heterobifunctional compounds, as described hereinmay have one or more double bonds that can exist as either the Z or Eisomer, unless otherwise indicated. The application additionallyencompasses the compounds as individual isomers substantially free ofother isomers and alternatively, as mixtures of various isomers, e.g.,racemic mixtures of stereoisomers. In addition to the above-mentionedcompounds per se, this application also encompasses pharmaceuticallyacceptable derivatives of these heterobifunctional compounds andcompositions comprising one or more compounds of the application and oneor more pharmaceutically acceptable excipients or additives.

Heterobifunctional compounds of the application may be prepared bycrystallization of the compound under different conditions and may existas one or a combination of polymorphs of the compound forming part ofthis application. For example, different polymorphs may be identifiedand/or prepared using different solvents, or different mixtures ofsolvents for recrystallization; by performing crystallizations atdifferent temperatures; or by using various modes of cooling, rangingfrom very fast to very slow cooling during crystallizations. Polymorphsmay also be obtained by heating or melting the compound followed bygradual or fast cooling. The presence of polymorphs may be determined bysolid probe NMR spectroscopy, IR spectroscopy, differential scanningcalorimetry, powder X-ray diffractogram and/or other techniques. Thus,the present application encompasses heterobifunctional compounds, theirderivatives, their tautomeric forms, their stereoisomers, theirpolymorphs, their pharmaceutically acceptable salts theirpharmaceutically acceptable solvates and pharmaceutically acceptablecompositions containing them.

General Synthesis of the Heterobifunctional Compounds

The heterobifunctional compounds described herein can be prepared bymethods known by those skilled in the art. In one non-limiting examplethe disclosed heterobifunctional compounds can be made by the followingschemes.

As shown in Scheme 1 heterobifunctional compounds for use in the presentinvention can be prepared by chemically combining a Degron and a Linkerfollowed by subsequent addition of a dTAG Targeting Ligand. Similarly,in Scheme 2 heterobifunctional compounds for use in the presentinvention are prepared by chemically combing a dTAG Targeting Ligand andLinker first, followed by subsequent addition of a Degron. Asillustrated in the above and following schemes, heterobifunctionalcompounds for use in the present invention can readily be synthesized byone skilled in the art in a variety of methods and chemical reactions.

Scheme 3: In Step 1, a nucleophilic Degron displaces a leaving group onthe Linker to make a Degron Linker fragment. In Step 2, the protectinggroup is removed by methods known in the art to free a nucleophilic siteon the Linker. In Step 3, the nucleophilic Degron Linker fragmentdisplaces a leaving group on the dTAG Targeting Ligand to form acompound for use in the present invention. In an alternative embodimentStep 1 and/or Step 2 is accomplished by a coupling reaction instead of anucleophilic attack.

Scheme 4: In Step 1, a nucleophilic dTAG Targeting Ligand displaces aleaving group on the Linker to make a dTAG Targeting Ligand Linkerfragment. In Step 2, the protecting group is removed by methods known inthe art to free a nucleophilic site on the Linker. In Step 3, thenucleophilic dTAG Targeting Ligand Linker fragment displaces a leavinggroup on the Degron to form a compound for use in the present invention.In an alternative embodiment Step 1 and/or Step 2 is accomplished by acoupling reaction instead of a nucleophilic attack.

Scheme 5 and Scheme 6: In Step 1, a nucleophilic Degron displaces aleaving group on the Linker to make a Degron Linker fragment. In Step 2,the protecting group is removed by methods known in the art to free anucleophilic site on the Linker. In Step 3, the nucleophilic DegronLinker fragment displaces a leaving group on the dTAG Targeting Ligandto form a compound of Formula I or Formula II. In an alternativeembodiment Step 1 and/or Step 2 is accomplished by a coupling reactioninstead of a nucleophilic attack.

In certain embodiments, the methods described above are carried out insolution phase. In certain other embodiments, the methods describedabove are carried out on a solid phase. In certain embodiments, thesynthetic method is amenable to high-throughput techniques or totechniques commonly used in combinatorial chemistry.

Representative Synthesis of the Heterobifunctional Compounds

Unless otherwise indicated, starting materials are either commerciallyavailable or readily accessible through laboratory synthesis by anyonereasonably familiar with the art. Described generally below, areprocedures and general guidance for the synthesis of compounds asdescribed generally and in subclasses and species herein.

Synthetic Example 1′: Synthesis of IMiD Derivatives and Degrons

General Procedure I: IMiD Condensation2-(2,6-dioxopiperidin-3-yl)-4-hydroxyisoindoline-1,3-dione (D-1)

In a 20 mL glass vial, a mixture of 3-hydroxyphthalic anhydride (500 mg,3.05 mmol, 1 equiv), potassium acetate (927 mg, 9.44 mmol, 3.1 equiv)and 3-aminopiperidine-2,6-dione hydrochloride (552 mg, 3.35 mmol, 1.1equiv) in acetic acid (10.2 mL, 0.3 M) was heated to 90° C. overnight.The black reaction mixture was cooled to room temperature and diluted to20 mL with water, and subsequently cooled on ice for 30 min. Theresulting slurry was transferred to a 50 mL Falcon tube, which wascentrifuged at 3500 rpm for 5 min. The supernatant was discarded and theblack solid was transferred to a 250 mL RBF with methanol andconcentrated in vacuo. The residue was purified by flash columnchromatography on silica gel (CH₂Cl₂:MeOH (9:1)) to afford the titlecompound as a white solid (619 mg, 74%). ¹H NMR (400 MHz, DMSO-d₆) δ11.07 (s, 1H), 7.65 (dd, J=8.4, 6.8 Hz, 1H), 7.31 (d, J=6.8 Hz, 1H),7.24 (d, J=8.4 Hz, 1H), 5.06 (dd, J=12.8, 5.4 Hz, 1H), 2.94-2.82 (m,1H), 2.64-2.43 (m, 2H), 2.08-1.97 (m, 1H); MS (ESI) calcd for C₁₃H₁₁N₂O₅[M+H]⁺ 275.07, found 275.26.

2-(2,6-dioxopiperidin-3-yl)-4-nitroisoindoline-1,3-dione (D-10)

General procedure I was followed using 3-nitrophthalic anhydride (300mg, 1.55 mmol, 1 equiv), potassium acetate (473 mg, 4.82 mmol, 3.1equiv) and 3-aminopiperidine-2,6-dione hydrochloride (281 mg, 1.71 mmol,1.1 equiv) to afford the title compound as a light yellow solid (280 mg,59%) following purification by flash column chromatography on silica gel(CH₂Cl₂:MeOH (9:1)). ¹H NMR (500 MHz, DMSO-d₆) δ 11.17 (s, 1H), 8.35 (d,J=8.1 Hz, 1H), 8.24 (d, J=7.5 Hz, 1H), 8.14-8.10 (m, 1H), 5.20 (dd,J=12.9, 5.5 Hz, 1H), 2.93-2.84 (m, 1H), 2.64-2.45 (m, 2H), 2.11-2.04 (m,1H); MS (ESI) calcd for C₁₃H₁₀N₃O₆ [M+H]⁺ 304.06, found 304.19.

2-(2,6-dioxopiperidin-3-yl)-5-nitroisoindoline-1,3-dione (D-2)

General procedure I was followed using 4-nitrophthalic anhydride (300mg, 1.55 mmol), potassium acetate (473 mg, 4.82 mmol) and3-aminopiperidine-2,6-dione hydrochloride (281 mg, 1.71 mmol) to affordthe title compound as a white solid (409 mg, 87%) following purificationby flash column chromatography on silica gel (CH₂Cl₂:MeOH (30:1)). ¹HNMR (500 MHz, DMSO-d₆) δ 11.18 (s, 1H), 8.68 (dd, J=8.1, 1.9 Hz, 1H),8.56 (d, J=1.9 Hz, 1H), 8.19 (d, J=8.1 Hz, 1H), 5.24 (dd, J=12.9, 5.4Hz, 1H), 2.90 (ddd, J=17.2, 13.9, 5.5 Hz, 1H), 2.69-2.48 (m, 2H),2.14-2.05 (m, 1H); MS (ESI) calcd for C₁₃H₁₀N₃O₆ [M+H]⁺ 304.06, found304.19.

2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione (D-6)

General procedure I was followed using phthalic anhydride (155 mg, 1.05mmol), potassium acetate (318 mg, 3.24 mmol) and3-aminopiperidine-2,6-dione hydrochloride (189 mg, 1.15 mmol) to affordthe title compound as a white solid (235 mg, 87%) following purificationby flash column chromatography on silica gel (CH₂Cl₂:MeOH (15:1)). ¹HNMR (500 MHz, DMSO-d₆) δ 11.13 (s, 1H), 8.00-7.76 (m, 4H), 5.16 (dd,J=12.8, 5.4 Hz, 1H), 2.89 (ddd, J=16.8, 13.7, 5.4 Hz, 1H), 2.65-2.42 (m,2H), 2.12-1.99 (m, 1H); MS (ESI) calcd for C₁₃H₁₁N2O₄ [M+H]⁺ 259.07,found 259.23.

2-(2,5-dioxopyrrolidin-3-yl)isoindoline-1,3-dione (D-7)

General procedure I was followed using phthalic anhydride (90 mg, 0.608mmol), potassium acetate (185 mg, 1.88 mmol) and3-aminopyrrolidine-2,5-dione hydrochloride (101 mg, 0.668 mmol) toafford the title compound as a white solid (95 mg, 64%) followingpurification by flash column chromatography on silica gel (CH₂Cl₂:MeOH(14:1)). MS (ESI) calcd for C₁₂H₉N₂O₄ [M+H]⁺ 245.06, found 245.26.

2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindoline-5-carboxylic acid(D-13)

General procedure I was followed using 1,2,4-benzenetricarboxylicanhydride (200 mg, 1.04 mmol), potassium acetate (317 mg, 3.23 mmol) and3-aminopiperidine-2,6-dione hydrochloride (188 mg, 1.15 mmol) to affordthe title compound as a white solid (178 mg, 57%) following purificationby flash column chromatography on silica gel (CH₂Cl₂:MeOH (9:1)). MS(ESI) calcd for C₁₄H₁₁N206 [M+H]⁺ 303.06, found 303.24.

2-(2,6-dioxopiperidin-3-yl)-4-fluoroisoindoline-1,3-dione (D-14)

General procedure I was followed using 3-fluorophthalic anhydride (200mg, 1.20 mmol), potassium acetate (366 mg, 3.73 mmol) and3-aminopiperidine-2,6-dione hydrochloride (218 mg, 1.32 mmol) to affordthe title compound as a white solid (288 mg, 86%) following purificationby flash column chromatography on silica gel (CH₂Cl₂:MeOH (50:1)). ¹HNMR (500 MHz, DMSO-d₆) δ 11.15 (s, 1H), 7.96 (ddd, J=8.3, 7.3, 4.5 Hz,1H), 7.82-7.71 (m, 2H), 5.17 (dd, J=13.0, 5.4 Hz, 1H), 2.90 (ddd,J=17.1, 13.9, 5.4 Hz, 1H), 2.65-2.47 (m, 2H), 2.10-2.04 (m, 1H), MS(ESI) calcd for C₁₃H₁₀FN₂O₄ [M+H]⁺ 277.06, found 277.25.

2-(2,6-dioxopiperidin-3-yl)-4-methylisoindoline-1,3-dione (D-19)

General procedure I was followed using 3-methylphthalic anhydride (150mg, 0.925 mmol), potassium acetate (281 mg, 2.87 mmol) and3-aminopiperidine-2,6-dione hydrochloride (167 mg, 1.02 mmol) to affordthe title compound as a white solid (168 mg, 67%) following purificationby flash column chromatography on silica gel (CH₂Cl₂:MeOH (15:1)). MS(ESI) calcd for C₁₄H₁₃N₂O₄ [M+H]⁺ 273.09, found 273.24.

2-(2,6-dioxopiperidin-3-yl)-5-fluoroisoindoline-1,3-dione (D-24)

General procedure I was followed using 4-fluorophthalic anhydride (200mg, 1.20 mmol), potassium acetate (366 mg, 3.73 mmol) and3-aminopiperidine-2,6-dione hydrochloride (218 mg, 1.32 mmol) to affordthe title compound as a white solid (254 mg, 76%) following purificationby flash column chromatography on silica gel (CH₂Cl₂:MeOH (15:1)). MS(ESI) calcd for C₁₃H₁₀FN₂O₄ [M+H]⁺ 277.06, found 277.24.

2-(2,6-dioxopiperidin-4-yl)isoindoline-1,3-dione (D-43)

General procedure I was followed using phthalic anhydride (60 mg, 0.311mmol), potassium acetate (95 mg, 0.963 mmol) and4-aminopiperidine-2,6-dione hydrochloride (56 mg, 0.342 mmol) to affordthe title compound as a white solid (40 mg, 43%) following purificationby flash column chromatography on silica gel (CH₂Cl₂:MeOH (9:1)). MS(ESI) calcd for C₁₃H₁₁N₂O₄ [M+H]⁺ 259.07, found 259.18.

General Procedure II: Reduction of Aromatic Nitro Groups4-amino-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione (D-4)

A solution of 2-(2,6-dioxopiperidin-3-yl)-4-nitroisoindoline-1,3-dione(173 mg, 0.854 mmol), Pd(OAc)₂ (12.8 mg, 0.0854 mmol, 10 mol %) andpotassium fluoride (66 mg, 1.71 mmol, 2 equiv) in THF:water (8:1) (5.7mL, 0.1 M) was stirred at room temperature. Triethylsilane (365 μL, 3.41mmol, 4 equiv) was added slowly, and the resulting black solution wasstirred at room temperature for 1 hour. The reaction mixture wasfiltered through a pad of celite, which was washed excessively withethyl acetate. The filtrate was concentrated in vacuo and the residuewas purified by flash column chromatography on silica gel (CH₂Cl₂:MeOH(7:1)) to afford the title compound as a yellow powder (72 mg, 46%). ¹HNMR (500 MHz, DMSO-d₆) δ 11.08 (s, 1H), 7.47 (dd, J=8.5, 7.0 Hz, 1H),7.06-6.95 (m, 1H), 6.59-6.44 (m, 1H), 5.04 (dd, J=12.7, 5.4 Hz, 1H),2.93-2.82 (m, 1H), 2.64-2.45 (m, 2H), 2.05-1.98 (m, 1H); MS (ESI) calcdfor C₁₃H₁₁N₃O₄ [M+H]⁺ 274.08, found 274.23.

2-(2,6-dioxopiperidin-3-yl)-5-nitroisoindoline-1,3-dione (D-8)

General procedure II was followed using2-(2,6-dioxopiperidin-3-yl)-5-nitroisoindoline-1,3-dione (100 mg, 0.330mmol), Pd(OAc)₂ (7.4 mg, 0.033 mmol), potassium fluoride (38 mg, 0.660mmol) and triethylsilane (211 μL, 1.32 mmol to afford the title compoundas a yellow solid (33 mg, 37%) following purification by flash columnchromatography on silica gel (CH₂Cl₂:MeOH (9:1)). ¹H NMR (500 MHz,DMSO-d₆) δ 11.05 (s, 1H), 7.52 (d, J=8.2 Hz, 1H), 6.94 (d, J=2.0 Hz,1H), 6.83 (dd, J=8.2, 2.0 Hz, 1H), 6.55 (s, 2H), 5.01 (dd, J=12.8, 5.4Hz, 1H), 2.86 (ddd, J=16.9, 13.9, 5.5 Hz, 1H), 2.68-2.43 (m, 2H),2.03-1.93 (m, 1H); MS (ESI) calcd for C₁₃H₁₂N₃O₄ [M+H]⁺ 274.08, found274.59.

4-amino-2-(1-benzyl-2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione (D-12)

General procedure II was followed using2-(1-benzyl-2,6-dioxopiperidin-3-yl)-4-nitroisoindoline-1,3-dione (48mg, 0.122 mmol), Pd(OAc)₂ (2.7 mg, 0.0122 mmol), potassium fluoride (14mg, 0.244 mmol) and triethylsilane (78 μL, 0.488 mmol to afford thetitle compound as a yellow solid (7 mg, 16%) following purification byflash column chromatography on silica gel (0 to 100% EtOAc in hexanes).MS (ESI) calcd for C₂₀H₁₈N₃O₄ [M+H]⁺ 364.13, found 364.34.

3-(5-amino-2-methyl-4-oxoquinazolin-3(4H)-yl)piperidine-2,6-dione (D-17)

General procedure II was followed using3-(2-methyl-5-nitro-4-oxoquinazolin-3(4H)-yl)piperidine-2,6-dione (21mg, 0.0664 mmol), Pd(OAc)₂ (1.5 mg, 0.0066 mmol), potassium fluoride(7.7 mg, 0.133 mmol) and triethylsilane (42 μL, 0.266 mmol to afford thetitle compound as a white solid (7 mg, 37%) following purification bypreparative HPLC. MS (ESI) calcd for C₁₄H₁₅N₄O₃ [M+H]⁺ 287.11, found287.30.

3-(7-amino-1-oxoisoindolin-2-yl)piperidine-2,6-dione (D-41)

General procedure II was followed using3-(7-nitro-1-oxoisoindolin-2-yl)piperidine-2,6-dione (11 mg, 0.038mmol), Pd(OAc)₂ (0.9 mg, 0.0038 mmol), potassium fluoride (4.4 mg, 0.076mmol) and triethylsilane (24 μL, 0.152 mmol to afford the title compoundas a yellow solid (2 mg, 21%) following purification by flash columnchromatography on silica gel (0 to 10% MeOH in CH₂Cl₂). MS (ESI) calcdfor C₁₃H₁₄N₃O₃ [M+H]⁺ 260.10, found 260.52.

General Procedure III: Acylation of AnilinesN-(2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-5-yl)acetamide (D-5)

In a 4 mL glass vial, a mixture of5-amino-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione (30 mg, 0.110mmol, 1 equiv) and acetyl chloride (26 μL, 0.220 mmol, 2 equiv) in THF(1.8 mL, 0.1 M) was heated to reflux overnight. The reaction mixture wasfiltered, and the filter cake was washed with Et₂O to give the titlecompound as a white solid (27 mg, 47%), that was used without furtherpurification. ¹H NMR (500 MHz, DMSO-d₆) δ 11.11 (s, 1H), 10.63 (s, 1H),8.24 (d, J=1.5 Hz, 1H), 7.91-7.83 (m, 2H), 5.11 (dd, J=12.8, 5.4 Hz,1H), 2.88 (ddd, J=17.0, 13.8, 5.4 Hz, 1H), 2.63-2.46 (m, 2H), 2.13 (s,3H), 2.09-2.00 (m, 1H); MS (ESI) calcd for C₁₅H₁₄N₃O₅ [M+H]⁺ 316.09,found 316.23.

N-(2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)acetamide (D-3)

General procedure III was followed using4-amino-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione (50 mg, 0.183mmol) and acetyl chloride (26 μL, 0.366 mmol) to afford the titlecompound as a white solid (10 mg, 17%). ¹H NMR (500 MHz, DMSO-d₆) δ11.14 (s, 1H), 9.73 (s, 1H), 8.44 (d, J=8.4 Hz, 1H), 7.83 (dd, J=8.4,7.3 Hz, 1H), 7.62 (d, J=7.2 Hz, 1H), 5.14 (dd, J=12.9, 5.4 Hz, 1H), 2.90(ddd, J=17.1, 13.9, 5.4 Hz, 1H), 2.66-2.45 (m, 2H), 2.19 (s, 3H),2.14-2.00 (m, 1H); MS (ESI) calcd for C₁₅H₁₄N₃O₅ [M+H]⁺ 316.09, found316.27.

2-chloro-N-(2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-5-yl)acetamide(D-32)

General procedure III was followed using5-amino-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione (10 mg, 0.0366mmol) and chloroacetyl chloride (6 μL, 0.0732 mmol) to afford the titlecompound as a white solid (7.1 mg, 55%). MS (ESI) calcd for C₁₅H₃ClN₃O₅[M+H]⁺ 350.05, found 350.23.

2-chloro-N-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-4-yl)acetamide(D-34)

General procedure III was followed using3-(4-amino-1-oxoisoindolin-2-yl)piperidine-2,6-dione (20 mg, 0.0771mmol) and chloroacetyl chloride (12 μL, 0.154 mmol) to afford the titlecompound as a white solid (14.9 mg, 56%). ¹H NMR (500 MHz, DMSO-d₆) δ11.02 (s, 1H), 10.20 (s, 1H), 7.81 (dd, J=7.7, 1.3 Hz, 1H), 7.65-7.47(m, 2H), 5.16 (dd, J=13.3, 5.1 Hz, 1H), 4.45-4.34 (m, 2H), 4.33 (s, 2H),3.00-2.85 (m, 1H), 2.68-2.56 (m, 1H), 2.41-2.28 (m, 1H), 2.09-1.97 (m,1H); MS (ESI) calcd for C₁₅H15C₁N304 [M+H]⁺ 336.07, found 336.31.

N-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-4-yl)acrylamide (D-35)

General procedure III was followed using3-(4-amino-1-oxoisoindolin-2-yl)piperidine-2,6-dione (20 mg, 0.0771mmol) and acryloyl chloride (13 μL, 0.154 mmol) to afford the titlecompound as a white solid (18 mg, 76%). ¹H NMR (500 MHz, DMSO-d₆) δ15.77 (s, 1H), 14.81 (s, 1H), 12.65 (dd, J=7.4, 1.6 Hz, 1H), 12.37-12.18(m, 2H), 11.28 (dd, J=17.0, 10.2 Hz, 1H), 11.06 (dd, J=17.0, 1.9 Hz,1H), 10.57 (dd, J=10.2, 1.9 Hz, 1H), 9.91 (dd, J=13.3, 5.1 Hz, 1H),9.24-9.05 (m, 2H), 7.67 (ddd, J=17.2, 13.7, 5.5 Hz, 1H), 7.36 (dt,J=17.3, 3.8 Hz, 1H), 7.20-7.03 (m, 1H), 6.83-6.72 (m, 1H); MS (ESI)calcd for C₁₆H₁₆N₃O₄ [M+H]⁺ 314.11, found 314.24.

N-(2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-5-yl)acrylamide(D-36)

General procedure III was followed using5-amino-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione (10 mg, 0.0366mmol) and acryloyl chloride (6 μL, 0.0732 mmol) to afford the titlecompound as a white solid (8.8 mg, 73%). ¹H NMR (500 MHz, DMSO-d₆) δ11.12 (s, 1H), 10.83 (s, 1H), 8.33 (d, J=1.8 Hz, 1H), 7.99 (dd, J=8.2,1.9 Hz, 1H), 7.90 (d, J=8.2 Hz, 1H), 6.48 (dd, J=17.0, 10.1 Hz, 1H),6.36 (dd, J=17.0, 1.9 Hz, 1H), 5.88 (dd, J=10.0, 1.9 Hz, 1H), 5.13 (dd,J=12.8, 5.5 Hz, 1H), 2.95-2.84 (m, 1H), 2.67-2.46 (m, 2H), 2.09-2.01 (m,1H); MS (ESI) calcd for C₁₆H₁₄N₃O₅ [M+H]⁺ 328.09, found 328.23.

N-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-4-yl)acetamide (D-37)

General procedure III was followed using3-(4-amino-1-oxoisoindolin-2-yl)piperidine-2,6-dione (20 mg, 0.0771mmol) and acetyl chloride (11 μL, 0.154 mmol) to afford the titlecompound as a white solid (17 mg, 71%). MS (ESI) calcd for C₁₅H₁₆N₃O₄[M+H]⁺ 302.11, found 301.99.

N-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-4-yl)cyclopropanecarboxamide(D-38)

General procedure III was followed using3-(4-amino-1-oxoisoindolin-2-yl)piperidine-2,6-dione (20 mg, 0.0771mmol) and cyclopropanecarbonyl chloride (14 μL, 0.154 mmol) to affordthe title compound as a white solid (19 mg, 75%). ¹H NMR (500 MHz,DMSO-d₆) δ 11.01 (s, 1H), 10.06 (s, 1H), 7.84 (dd, J=7.2, 1.9 Hz, 1H),7.66-7.38 (m, 2H), 5.14 (dd, J=13.3, 5.1 Hz, 1H), 4.52-4.30 (m, 2H),2.92 (ddd, J=17.3, 13.6, 5.4 Hz, 1H), 2.64-2.54 (m, 1H), 2.45-2.27 (m,1H), 2.08-1.95 (m, 1H), 1.93-1.83 (m, 1H), 0.90-0.75 (m, 4H); MS (ESI)calcd for C₁₇H₁₈N₃O₄ [M+H]⁺ 328.13, found 328.00.

General Procedure IV: Quinazolinone Condensation3-(2-methyl-4-oxoquinazolin-3(4H)-yl)piperidine-2,6-dione (D-9)

In a 20 mL glass vial, anthranilic acid (100 mg, 0.729 mmol, 1 equiv),acetic acid (42 μL, 0.729 mmol, 1 equiv) and P(OPh)₃ (479 μL, 1.82 mmol,2.5 equiv) in pyridine (1.0 uL, 0.7 M) was heated to 90° C. After 4hours, the reaction mixture was cooled to room temperature and3-aminopiperidine-2,6-dione hydrochloride (144 mg, 0.875 mmol, 1.2equiv) was added. The reaction mixture was reheated to 90° C. for 1.5 h,whereupon it was stirred at room temperature overnight. The reactionmixture was taken up in EtOAc (15 mL) and water (15 mL). The organiclayer was washed with brine (2×25 mL), dried over Na₂SO₄ andconcentrated in vacuo. The residue was purified by flash columnchromatography on silica gel (0-5% MeOH in CH₂Cl₂) to afford the titlecompound as a white solid (79 mg, 40%). ¹H NMR (500 MHz, DMSO-d₆) δ11.03 (s, 1H), 8.03 (dd, J=7.9, 1.5 Hz, 1H), 7.82 (ddd, J=8.5, 7.1, 1.6Hz, 1H), 7.62 (dd, J=8.3, 1.1 Hz, 1H), 7.50 (ddd, J=8.1, 7.1, 1.1 Hz,1H), 5.27 (dd, J=11.5, 5.7 Hz, 1H), 2.92-2.78 (m, 1H), 2.73-2.56 (m,5H), 2.26-2.06 (m, 1H); MS (ESI) calcd for C₁₄H₁₄N₃O₃ [M+H]⁺ 272.10,found 272.33.

3-(2-methyl-4-oxoquinazolin-3(4H)-yl)pyrrolidine-2,5-dione (D-11)

General procedure IV was followed using anthranilic acid (200 mg, 1.46mmol), acetic acid (84 μL, 1.46 mmol), P(OPh)₃ (959 μL, 3.65 mmol) and3-aminopyrrolidine-2,5-dione hydrochloride (263 mg, 1.75 mmol) to affordthe title compound as a white solid (25 mg, 7%) following purificationby flash column chromatography on silica gel (CH₂Cl₂:MeOH (15:1)). MS(ESI) calcd for C₁₃H₁₂N₃O₃ [M+H]⁺ 258.09, found 258.22.

3-(5-fluoro-2-methyl-4-oxoquinazolin-3(4H)-yl)piperidine-2,6-dione(D-66)

General procedure IV was followed using 6-fluoro anthranilic acid (100mg, 0.645 mmol), acetic acid (37 μL, 0.644 mmol), P(OPh)₃ (424 μL, 1.61mmol) and 3-aminopiperidine-2,6-dione hydrochloride (127 mg, 0.774 mmol)to afford the title compound as a white solid (70 mg, 38%) followingpurification by flash column chromatography on silica gel (0-10% MeOH inCH₂Cl₂). ¹H NMR (500 MHz, DMSO-d₆) δ 11.03 (s, 1H), 7.84-7.76 (m, 1H),7.44 (dd, J=8.2, 1.0 Hz, 1H), 7.25 (ddd, J=11.1, 8.2, 1.0 Hz, 1H), 5.24(dd, J=11.3, 5.7 Hz, 1H), 2.90-2.75 (m, 1H), 2.62 (s, 3H), 2.61-2.56 (m,2H), 2.20-2.12 (m, 1H); MS (ESI) calcd for C₁₄H₁₃FN₃O₃ [M+H]⁺ 290.09,found 290.27.

3-(2-methyl-5-nitro-4-oxoquinazolin-3(4H)-yl)piperidine-2,6-dione (D-67)

General procedure IV was followed using 6-nitroanthranilic acid (100 mg,0.549 mmol), acetic acid (31 μL, 0.549 mmol), P(OPh)₃ (361 μL, 1.37mmol) and 3-aminopiperidine-2,6-dione hydrochloride (108 mg, 0.659 mmol)to afford the title compound as a white solid (29 mg, 17%) followingpurification by flash column chromatography on silica gel (0-10% MeOH inCH₂Cl₂). MS (ESI) calcd for C₁₄H₁₃N₄O₅ [M+H]⁺ 317.09, found 317.58.

General Procedure V: Amide CouplingN-benzyl-2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindoline-5-carboxamide(D-15)

In a 4 mL glass vial,2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindoline-5-carboxylic acid (10mg, 0.033 mmol, 1 equiv), HATU (13 mg, 0.033 mmol, 1 equiv), DIPEA (17μL, 0.099 mmol, 3 equiv) and benzyl amine (4 μL, 0.036 mmol, 1.1 equiv)in DMF (331 μL, 0.1 M) was stirred at room temperature overnight. Thereaction mixture was diluted with MeOH to 4 mL, filtered and thenpurified by preparative HPLC to afford the title compound as a whitesolid (6 mg, 46%). MS (ESI) calcd for C₂₁H₁₈N₃O₅ [M+H]⁺ 392.12, found392.33.

General Procedure VI: Nucleophilic Aromatic Substitution4-(benzylamino)-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione (D-16)

In a 4 mL glass vial,2-(2,6-dioxopiperidin-3-yl)-4-fluoroisoindoline-1,3-dione (10 mg, 0.036mmol, 1 equiv), benzyl amine (4.4 μL, 0.040 mmol, 1.1 equiv) and DIPEA(13 μL, 0.072 mmol, 2 equiv) in NMP (362 μL, 0.1 M) was heated to 90° C.overnight. The reaction mixture was cooled to room temperature and takenup in EtOAc (15 mL). The organic layer was washed with NaHCO₃(aq) (15mL), water (15 mL) and brine (3×15 mL), and subsequently dried overNa₂SO₄ and concentrated in vacuo. The residue was purified by flashcolumn chromatography on silica gel (0-100% EtOAc in hexanes) to affordthe title compound as a yellow film (5 mg, 38%). ¹H NMR (500 MHz,Chloroform-d) δ 8.10 (s, 1H), 7.44 (dd, J=8.5, 7.1 Hz, 1H), 7.40-7.25(m, 5H), 7.12 (d, J=7.1 Hz, 1H), 6.84 (d, J=8.5 Hz, 1H), 6.71 (t, J=5.9Hz, 1H), 4.93 (dd, J=12.3, 5.3 Hz, 1H), 4.51 (d, J=5.9 Hz, 2H),2.93-2.66 (m, 3H), 2.21-2.07 (m, 1H); MS (ESI) calcd for C₂₀H₁₈N₃O₄[M+H]⁺ 364.13, found 364.31.

2-(2,6-dioxopiperidin-3-yl)-4-(isopropylamino)isoindoline-1,3-dione(D-18)

General procedure VI was followed using2-(2,6-dioxopiperidin-3-yl)-4-fluoroisoindoline-1,3-dione (30 mg, 0.109mmol), isopropylamine (10 μL, 0.119 mmol) and DIPEA (21 jaL, 0.119 mmol)to afford the title compound as a yellow film (11 mg, 32%) followingpurification by flash column chromatography on silica gel (0-100% EtOAcin hexanes). MS (ESI) calcd for C₁₆H₁₈N₃O₄ [M+H]⁺ 316.13, found 316.65.

4-(diethylamino)-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione (D-21)

General procedure VI was followed using2-(2,6-dioxopiperidin-3-yl)-4-fluoroisoindoline-1,3-dione (30 mg, 0.109mmol), diethylamine (11 μL, 0.130 mmol) and DIPEA (32 μL, 0.181 mmol) toafford the title compound as a yellow film (28 mg, 97%) followingpurification by flash column chromatography on silica gel (0-100% EtOAcin hexanes). MS (ESI) calcd for C₁₇H₂₀N₃₀O₄ [M+H]⁺ 330.14, found 330.62.

5-(benzylamino)-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione (D-25)

General procedure VI was followed using2-(2,6-dioxopiperidin-3-yl)-5-fluoroisoindoline-1,3-dione (30 mg, 0.109mmol), benzyl amine (13 μL, 0.119 mmol) and DIPEA (38 μL, 0.217 mmol) toafford the title compound as a yellow film (6 mg, 15%) followingpurification by flash column chromatography on silica gel (0-100% EtOAcin hexanes). MS (ESI) calcd for C₂₀H₁₈N₃O₄ [M+H]⁺ 364.13, found 364.34.

2-(2,6-dioxopiperidin-3-yl)-5-(isopropylamino)isoindoline-1,3-dione(D-26)

General procedure VI was followed using2-(2,6-dioxopiperidin-3-yl)-5-fluoroisoindoline-1,3-dione (30 mg, 0.109mmol), isopropyl amine (11 μL, 0.130 mmol) and DIPEA (38 μL, 0.217 mmol)to afford the title compound as a yellow film (6 mg, 17%) followingpurification by flash column chromatography on silica gel (0-100% EtOAcin hexanes). ¹H NMR (500 MHz, Chloroform-d) 8.00 (s, 1H), 7.53 (d, J=8.3Hz, 1H), 6.87 (d, J=2.1 Hz, 1H), 6.64 (dd, J=8.3, 2.2 Hz, 1H), 4.86 (dd,J=12.3, 5.4 Hz, 1H), 4.30 (d, J=7.8 Hz, 1H), 2.86-2.58 (m, 3H),2.12-2.01 (m, 1H), 1.26-1.15 (m, 6H); MS (ESI) calcd for C₁₆H₁₈N₃O₄[M+H]⁺ 316.13, found 316.30.

5-(diethylamino)-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione (D-27)

General procedure VI was followed using2-(2,6-dioxopiperidin-3-yl)-5-fluoroisoindoline-1,3-dione (30 mg, 0.109mmol), diethylamine (14 μL, 0.130 mmol) and DIPEA (38 μL, 0.217 mmol) toafford the title compound as a yellow film (6 mg, 31%) followingpurification by flash column chromatography on silica gel (0-100% EtOAcin hexanes). ¹H NMR (500 MHz, Chloroform-d) δ 8.08 (s, 1H), 7.57 (d,J=8.6 Hz, 1H), 6.98 (d, J=2.4 Hz, 1H), 6.72 (dd, J=8.7, 2.4 Hz, 1H),4.90-4.80 (m, 1H), 3.40 (q, J=7.1 Hz, 4H), 2.89-2.61 (m, 3H), 2.11-2.01(m, 1H), 1.16 (t, J=7.1 Hz, 6H); MS (ESI) calcd for C₁₇H₂₀N₃O₄ [M+H]⁺330.14, found 330.69.

2-(2,6-dioxopiperidin-3-yl)-5-((furan-2-ylmethyl)amino)isoindoline-1,3-dione(D-28)

General procedure VI was followed using2-(2,6-dioxopiperidin-3-yl)-5-fluoroisoindoline-1,3-dione (50 mg, 0.181mmol), furfurylamine (18 μL, 0.199 mmol) and DIPEA (63 μL, 0.362 mmol)to afford the title compound as a yellow film (8 mg, 13%) followingpurification by flash column chromatography on silica gel (0-5% MeOH inCH₂Cl₂). MS (ESI) calcd for C₁₈H₁₆N₃O₄ [M+H]⁺ 354.11, found 354.25.

tert-butyl(2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)amino)ethyl)carbamate(D-29)

General procedure VI was followed using2-(2,6-dioxopiperidin-3-yl)-4-fluoroisoindoline-1,3-dione (50 mg, 0.181mmol), 1-Boc-ethylendiamine (32 mg, 0.199 mmol) and DIPEA (63 μL, 0.362mmol) to afford the title compound as a yellow film (31 mg, 41%)following purification by flash column chromatography on silica gel(0-10% MeOH in CH₂Cl₂). ¹H NMR (500 MHz, CDCl₃) δ 8.08 (bs, 1H), 7.50(dd, J=8.5, 7.1 Hz, 1H), 7.12 (d, J=7.1 Hz, 1H), 6.98 (d, J=8.5 Hz, 1H),6.39 (t, J=6.1 Hz, 1H), 4.96-4.87 (m, 1H), 4.83 (bs, 1H), 3.50-3.41 (m,2H), 3.41-3.35 (m, 2H), 2.92-2.66 (m, 3H), 2.16-2.09 (m, 1H), 1.45 (s,9H); MS (ESI) calcd for C₂₀H₂₅N₄O₆ [M+H]⁺ 417.18, found 417.58.

tert-butyl(2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-5-yl)amino)ethyl)carbamate(D-30)

General procedure VI was followed using2-(2,6-dioxopiperidin-3-yl)-5-fluoroisoindoline-1,3-dione (50 mg, 0.181mmol), 1-Boc-ethylendiamine (32 mg, 0.199 mmol) and DIPEA (63.L, 0.362mmol) to afford the title compound as a yellow film (22 mg, 29%)following purification by flash column chromatography on silica gel(0-10% MeOH in CH₂Cl₂). MS (ESI) calcd for C₂₀H₂₅N₄₀O₆ [M+H]⁺ 417.18,found 417.32.

2-(2,6-dioxopiperidin-3-yl)-4-((furan-2-ylmethyl)amino)isoindoline-1,3-dione(D-31)

General procedure VI was followed using2-(2,6-dioxopiperidin-3-yl)-4-fluoroisoindoline-1,3-dione (19.5 mg,0.0706 mmol), furfurylamine (7 μL, 0.078 mmol) and DIPEA (25 μL, 0.141mmol) to afford the title compound as a yellow film (19 mg, 76%)following purification by flash column chromatography on silica gel(0-2.5% MeOH in CH₂Cl₂). MS (ESI) calcd for C₁₈H₁₆N₃O₄ [M+H]⁺ 354.11,found 354.27.

3-(5-(benzylamino)-2-methyl-4-oxoquinazolin-3(4H)-yl)piperidine-2,6-dione(D-39)

With the exception that the reaction mixture was heated to 170° C.instead of 90° C., general procedure VI was followed using3-(5-fluoro-2-methyl-4-oxoquinazolin-3(4H)-yl)piperidine-2,6-dione (30mg, 0.104 mmol), benzylamine (13 μL, 0.114 mmol) and DIPEA (36 μL, 0.207mmol) to afford the title compound as a white solid (15 mg, 38%)following purification by flash column chromatography on silica gel(0-10% MeOH in CH₂Cl₂). ¹H NMR (500 MHz, Chloroform-d) δ 8.73 (t, J=5.7Hz, 1H), 8.39 (s, 1H), 7.41 (t, J=8.1 Hz, 1H), 7.39-7.19 (m, 5H), 6.77(d, J=7.7 Hz, 1H), 6.41 (d, J=8.3 Hz, 1H), 4.67 (dd, J=11.5, 5.9 Hz,1H), 4.43 (d, J=5.7 Hz, 2H), 3.03-2.79 (m, 2H), 2.72-2.61 (m, 1H), 2.60(s, 3H), 2.15-2.07 (m, 1H); MS (ESI) calcd for C₂₁H₂₁N₄O₃ [M+H]⁺ 377.16,found 377.02.

3-(5-(isopropylamino)-2-methyl-4-oxoquinazolin-3(4H)-yl)piperidine-2,6-dione(D-40)

With the exception that the reaction mixture was heated to 170° C.instead of 90° C., general procedure VI was followed using3-(5-fluoro-2-methyl-4-oxoquinazolin-3(4H)-yl)piperidine-2,6-dione (30mg, 0.104 mmol), isopropylamine (10 μL, 0.114 mmol) and DIPEA (36 μL,0.207 mmol) to afford the title compound as a white solid (5 mg, 15%)following purification by flash column chromatography on silica gel(0-10% MeOH in CH₂Cl₂). ¹H NMR (500 MHz, Chloroform-d) δ 8.31 (s, 1H),8.21 (d, J=7.2 Hz, 1H), 7.50-7.37 (m, 1H), 6.70 (dd, J=7.9, 0.9 Hz, 1H),6.47 (d, J=8.4 Hz, 1H), 4.65 (dd, J=11.4, 5.9 Hz, 1H), 3.69-3.56 (m,1H), 3.03-2.80 (m, 3H), 2.58 (s, 3H), 2.14-2.03 (m, 1H), 1.27 (d, J=2.7Hz, 3H), 1.26 (d, J=2.7 Hz, 3H); MS (ESI) calcd for C₁₇H₂₁N₄O₃ [M+H]⁺329.16, found 329.97.

2-(2,6-dioxopiperidin-3-yl)-4-((2-hydroxyethyl)amino)isoindoline-1,3-dione(D-68)

General procedure VI was followed using2-(2,6-dioxopiperidin-3-yl)-4-fluoroisoindoline-1,3-dione (30 mg, 0.109mmol), aminoethanol (7 jaL, 0.119 mmol) and DIPEA (38 μL, 0.217 mmol) toafford the title compound as a yellow film (6 mg, 18%) followingpurification by flash column chromatography on silica gel (0-5% MeOH inCH₂Cl₂). ¹H NMR (500 MHz, Chloroform-d) δ 8.26 (s, 1H), 7.50 (dd, J=8.5,7.1 Hz, 1H), 7.12 (d, J=7.0 Hz, 1H), 6.95 (d, J=8.5 Hz, 1H), 6.50 (t,J=5.9 Hz, 1H), 4.97-4.85 (m, 1H), 3.94-3.79 (m, 2H), 3.47 (q, J=5.5 Hz,2H), 3.03-2.68 (m, 3H), 2.19-2.04 (m, 1H); MS (ESI) calcd for C₁₅H6N₃O₅[M+H]⁺ 318.11, found 318.22.

4-(cyclopropylamino)-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione(D47)

General procedure VI was followed using2-(2,6-dioxopiperidin-3-yl)-4-fluoroisoindoline-1,3-dione (20 mg, 0.0724mmol), cyclopropylamine (6 μL, 0.080 mmol) and DIPEA (25 μL, 0.141 mmol)to afford the title compound as a yellow film (16 mg, 70%) followingpurification by flash column chromatography on silica gel (0-5% MeOH inCH₂Cl₂). ¹H NMR (500 MHz, Chloroform-d) 6 8.05 (s, 1H), 7.53 (dd, J=8.5,7.1 Hz, 1H), 7.33-7.21 (m, 1H), 7.15 (dd, J=7.1, 0.7 Hz, 1H), 6.44 (bs,1H), 4.95-4.85 (m, 1H), 2.98-2.66 (m, 3H), 2.62-2.50 (m, 1H), 2.19-2.06(m, 1H), 0.92-0.78 (m, 2H), 0.67-0.56 (m, 2H); MS (ESI) calcd forC₁₆H₁₆N₃O₄ [M+H]⁺ 314.11, found 314.54.

4-((2-(1H-indol-3-yl)ethyl)amino)-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione(D-48)

General procedure VI was followed using2-(2,6-dioxopiperidin-3-yl)-4-fluoroisoindoline-1,3-dione (20 mg, 0.0724mmol), tryptamine (13 mg, 0.080 mmol) and DIPEA (25 μL, 0.144 mmol) toafford the title compound as a yellow film (10 mg, 33%) followingpurification by flash column chromatography on silica gel (0-10% MeOH inCH₂Cl₂). ¹H NMR (500 MHz, Chloroform-d) δ 8.14 (s, 1H), 8.11 (s, 1H),7.65-7.55 (m, 1H), 7.45 (dd, J=8.6, 7.1 Hz, 1H), 7.37 (dt, J=8.2, 0.9Hz, 1H), 7.21 (ddd, J=8.2, 7.0, 1.2 Hz, 1H), 7.16-7.04 (m, 3H), 6.88 (d,J=8.5 Hz, 1H), 6.34 (t, J=5.6 Hz, 1H), 4.89 (dd, J=12.4, 5.4 Hz, 1H),3.59 (td, J=6.8, 5.5 Hz, 2H), 3.19-3.03 (m, 2H), 2.93-2.64 (m, 3H),2.14-2.04 (m, 1H); MS (ESI) calcd for C₂₃H₂₁N₄O₄ [M+H]⁺ 417.16, found417.26.

2-(2,6-dioxopiperidin-3-yl)-4-((4-hydroxyphenethyl)amino)isoindoline-1,3-dione(D-49)

General procedure VI was followed using2-(2,6-dioxopiperidin-3-yl)-4-fluoroisoindoline-1,3-dione (20 mg, 0.0724mmol), tyramine (11 mg, 0.080 mmol) and DIPEA (25 μL, 0.144 mmol) toafford the title compound as a yellow film (15 mg, 54%) followingpurification by flash column chromatography on silica gel (0-5% MeOH inCH₂Cl₂). ¹H NMR (500 MHz, Chloroform-d) δ 8.20 (s, 1H), 7.51 (dd, J=8.5,7.1 Hz, 1H), 7.17-7.08 (m, 2H), 6.90 (d, J=8.5 Hz, 1H), 6.85-6.72 (m,2H), 4.95-4.90 (m, 1H), 3.52-3.46 (m, 2H), 2.97-2.87 (m, 2H), 2.86-2.72(m, 2H), 2.21-2.09 (m, 1H); MS (ESI) calcd for C₂₁H₂₀N₃O₅ [M+H]⁺ 394.14,found 394.25.

4-((2-(1H-imidazol-2-yl)ethyl)amino)-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione(D-50)

General procedure VI was followed using2-(2,6-dioxopiperidin-3-yl)-4-fluoroisoindoline-1,3-dione (20 mg, 0.0724mmol), histamine (15 mg, 0.080 mmol) and DIPEA (25 μL, 0.144 mmol) toafford the title compound as a yellow film (5 mg, 19%) followingpurification by flash column chromatography on silica gel (0-10% MeOH inCH₂Cl₂). ¹H NMR (500 MHz, Chloroform-d) δ 8.19 (s, 1H), 7.61 (d, J=1.2Hz, 1H), 7.47 (dd, J=8.5, 7.1 Hz, 1H), 7.07 (d, J=6.9 Hz, 1H), 6.96-6.83(m, 2H), 6.39 (t, J=5.7 Hz, 1H), 4.97-4.79 (m, 1H), 3.59 (q, J=6.5 Hz,2H), 2.95 (t, J=6.6 Hz, 2H), 2.92-2.62 (m, 2H), 2.16-2.04 (m, 1H); MS(ESI) calcd for C₁₈H₁₁N₅O₄ [M+H]⁺ 368.14, found 368.47.

General Procedure VII: Acylation of Primary AminesN-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)methyl)cyclopropanecarboxamide(D-22)

In a 4 mL glass vial,4-(aminomethyl)-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione (25 mg,0.087 mmol, 1 equiv) and DIPEA (30 μL, 0.174 mmol, 2 equiv) in MeCN (250μL, 0.35 M) was cooled to 0° C. Cyclopropanecarbonyl chloride (8.7 μL,0.096 mmol) was added slowly and the reaction mixture was stirred atroom temperature overnight. The product was isolated by filtration toafford the title compound as a white solid (4.8 mg, 15%), that was usedwithout further purification. MS (ESI) calcd for C₁₈H₁₈N₃O₅ [M+H]⁺356.12, found 356.32.

N-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)methyl)acetamide(D-23)

General procedure VII was followed using4-(aminomethyl)-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione (25 mg,0.087 mmol), DIPEA (30 μL, 0.174 mmol) and acetyl chloride (7 μL, 0.096mmol) to afford the title compound as a white solid (4.5 mg, 16%). ¹HNMR (500 MHz, DMSO-d₆) δ 11.13 (s, 1H), 8.47 (t, J=6.0 Hz, 1H),7.88-7.76 (m, 2H), 7.70 (dt, J=7.3, 1.1 Hz, 1H), 5.15 (dd, J=12.7, 5.4Hz, 1H), 4.69 (d, J=6.0 Hz, 2H), 2.90 (ddd, J=16.8, 13.8, 5.4 Hz, 1H),2.64-2.44 (m, 2H), 2.15-2.01 (m, 1H), 1.92 (s, 3H); MS (ESI) calcd forC₁₆H₁₆N₃O₅ [M+H]⁺ 330.11, found 330.05.

2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)amino)ethan-1-aminium2,2,2-trifluoroacetate (D-33)

A stirred solution of tert-butyl(2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)amino)ethyl)carbamate(205 mg, 0.492 mmol, 1 equiv) in dichloromethane (2.25 mL) was addedtrifluoroacetic acid (0.250 mL). The reaction mixture was stirred atroom temperature for 4 h, whereupon the volatiles were removed in vacuo.The title compound was obtained as a yellow solid (226 mg, >95%), thatwas used without further purification. ¹H NMR (500 MHz, MeOD) δ 7.64 (d,J=1.4 Hz, 1H), 7.27-7.05 (m, 2H), 5.10 (dd, J=12.5, 5.5 Hz, 1H), 3.70(t, J=6.0 Hz, 2H), 3.50-3.42 (m, 2H), 3.22 (t, J=6.0 Hz, 1H), 2.93-2.85(m, 1H), 2.80-2.69 (m, 2H), 2.17-2.10 (m, 1H); MS (ESI) calcd forC₁₅H₁₇N₄O₄ [M+H]⁺ 317.12, found 317.53.

General Procedure VIII: Phenol Alkylation2-(2,6-dioxopiperidin-3-yl)-4-((4-(morpholinomethyl)benzyl)oxy)isoindoline-1,3-dione(D-45)

In a 4 mL glass vial,2-(2,6-dioxopiperidin-3-yl)-4-hydroxyisoindoline-1,3-dione (30 mg, 0.109mmol, 1 equiv) and K₂CO₃ (15 mg, 0.109 mmol, 1 equiv) in DMF (365 μL,0.3 M) was stirred at room temperature.4-(4-(bromomethyl)benzyl)morpholine (30 mg, 0.109 mmol, 1 equiv) in DMF(200 μL) was added and the reaction mixture was stirred at roomtemperature for 4 days. The reaction mixture was taken up in water (15mL) and EtOAc (15 mL), and the organic layer was washed with brine (3×15mL), dried over Na₂SO₄ and concentrated in vacuo. The residue waspurified by flash column chromatography on silica gel (0 to 10% MeOH inCH₂Cl₂) to afford the title compound as a white solid (20 mg, 40%). ¹HNMR (500 MHz, DMSO-d₆) δ 11.10 (s, 1H), 7.82 (dd, J=8.5, 7.2 Hz, 1H),7.60 (d, J=8.5 Hz, 1H), 7.50-7.42 (m, 3H), 7.35 (d, J=8.1 Hz, 2H), 5.35(s, 2H), 5.09 (dd, J=12.8, 5.5 Hz, 1H), 3.64-3.51 (m, 4H), 3.46 (s, 2H),2.88 (ddd, J=17.0, 14.1, 5.4 Hz, 1H), 2.63-2.47 (m, 2H), 2.38-2.31 (m,4H), 2.07-1.99 (m, 1H); MS (ESI) calcd for C₂₅H₂₆N₃O₆ [M+H]⁺ 464.18,found 464.00.

4-(benzyloxy)-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione (D-46)

General procedure VIII was followed using2-(2,6-dioxopiperidin-3-yl)-4-hydroxyisoindoline-1,3-dione (30 mg, 0.109mmol), K₂CO₃ (15 mg, 0.109 mmol) and benzyl bromide (8 μL, 0109 mmol) toafford the title compound as a white solid (8 mg, 20%) afterpurification by flash column chromatography on silica gel (0 to 10% MeOHin CH₂Cl₂). ¹H NMR (500 MHz, DMSO-d₆) δ 11.10 (s, 1H), 7.83 (dd, J=8.5,7.3 Hz, 1H), 7.60 (d, J=8.5 Hz, 1H), 7.53-7.50 (m, 2H), 7.47 (d, J=7.2Hz, 1H), 7.45-7.39 (m, 2H), 7.38-7.32 (m, 1H), 5.38 (s, 2H), 5.09 (dd,J=12.8, 5.5 Hz, 1H), 2.88 (ddd, J=16.9, 13.8, 5.5 Hz, 1H), 2.64-2.46 (m,2H), 2.07-1.99 (m, 1H); MS (ESI) calcd for C₂₀H₁₇N₂O₅ [M+H]⁺ 365.11,found 365.21.

2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)amino)ethyl4-methylbenzene-sulfonate (D-44)

In a 4 mL glass vial,2-(2,6-dioxopiperidin-3-yl)-4-((2-hydroxyethyl)amino)isoindoline-1,3-dione(7 mg, 0.0221 mmol, 1 equiv) and Et₃N (3 μL, 0.033 mmol, 1.5 equiv) inCH₂Cl₂ (200 μL) was stirred at room temperature. Tosyl chloride (6 mg,0.026 mmol, 1.2 equiv) in CH₂Cl₂ (100 μL) was added, and the reactionmixture was stirred at room temperature overnight. The reaction mixturewas concentrated in vacuo and the residue was purified by flash columnchromatography on silica gel (0-10% MeOH in CH₂Cl₂) to afford the titlecompound as a white solid (4 mg, 40%). ¹H NMR (500 MHz, DMSO-d₆) δ 11.13(s, 1H), 7.64-7.59 (m, 2H), 7.46 (dd, J=8.6, 7.1 Hz, 1H), 7.33-7.27 (m,2H), 7.04-6.93 (m, 2H), 6.58 (t, J=6.4 Hz, 1H), 5.09 (dd, J=12.7, 5.4Hz, 1H), 4.15 (t, J=5.1 Hz, 2H), 3.65-3.52 (m, 2H), 2.97-2.83 (m, 1H),2.67-2.46 (m, 2H), 2.27 (s, 3H), 2.12-2.02 (m, 1H); MS (ESI) calcd forC₂₂H₂₂N₃O₇S [M+H]⁺ 472.12, found 472.39.

(R)-4-hydroxy-2-(3-methyl-2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione(D-52)

Hydroxyisobenzofuran-1,3-dione (147.08 mg, 0.896 mmol, 1 eq) was addedto (R)-3-amino-3-methylpiperidine-2,6-dione hydrochloric acid (127.32mg, 0.896 mmol, 1 eq). Pyridine (3.584 ml, 0.25 M) was then added to themixture and it was stirred at 110° C. for 17 hours. The mixture wasdiluted with methanol and was condensed under reduced pressure. Thecrude material was purified by column chromatography (ISCO, 24 g silicacolumn, 0 to 10% MeOH/DCM 25 minute gradient) to give a white oil (110.9mg, 42.63% yield). ¹H NMR (400 MHz, DMSO-d₆) δ 10.95 (s, 1H), 7.61 (dd,J=8.4, 7.2 Hz, 1H), 7.27-7.14 (m, 2H), 2.73-2.63 (m, 1H), 2.57-2.51 (m,1H), 2.04-1.97 (m, 1H), 1.86 (s, 3H).

LCMS 289 (M+H).

(S)-4-hydroxy-2-(3-methyl-2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione(D-53)

4-hydroxyisobenzofuran-1,3-dione (148.99 mg, 0.907 mmol, 1 eq) was addedto (S)-3-amino-3-methylpiperidine-2,6-dione hydrochloric acid (128.97mg, 0.907 mmol, 1 eq). Pyridine (3.628 ml, 0.25 M) was then added to themixture and it was stirred at 110° C. for 17 hours. The mixture wasdiluted with methanol and was condensed under reduced pressure. Thecrude material was purified by column chromatography (ISCO, 24 g silicacolumn, 0 to 10% MeOH/DCM 25 minute gradient) to give a white oil (150mg, 57.4% yield). ¹H NMR (400 MHz, DMSO-d₆) δ 10.95 (s, 1H), 7.62 (dd,J=8.4, 7.2 Hz, 1H), 7.27-7.16 (m, 2H), 2.75-2.62 (m, 1H), 2.55 (dd,J=14.0, 4.3 Hz, 1H), 2.05-1.96 (m, 1H), 1.86 (s, 3H). LCMS 289 (M+H).

(S)-2-((2-(3-methyl-2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)aceticacid (D-55)

TFA (0.63 ml, 0.1 M) was added to tert-butyl(S)-2-((2-(3-methyl-2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetate(25.4 mg, 0.063 mmol, 1 eq) and the mixture was stirred at 50° C. for anhour. The mixture was then diluted with methanol and condensed underreduced pressure to give a white powder (20.5 mg, 93.9% yield) that wascarried forward without further purification. ¹H NMR (500 MHz,Methanol-d4) δ 7.81-7.75 (m, 1H), 7.50 (d, J=7.3 Hz, 1H), 7.45 (d, J=8.6Hz, 2H), 7.43-7.37 (m, 3H), 5.09 (dd, J=12.8, 5.5 Hz, 1H), 4.76 (s, 2H),4.63 (dd, J=9.1, 5.2 Hz, 1H), 3.66-3.55 (m, 30H), 3.51-3.41 (m, 5H),2.90-2.83 (m, 1H), 2.79-2.71 (m, 2H), 2.69 (s, 3H), 2.43 (s, 3H), 2.14(ddt, J=10.5, 5.5, 3.2 Hz, 1H), 1.69 (s, 3H). LCMS 347 (M+H).

(R)-2-((2-(3-methyl-2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)aceticacid (D-54)

TFA (1.78 ml, 0.1 M) was added to tert-butyl(R)-2-((2-(3-methyl-2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetate(71.3 mg, 0.178 mmol, 1 eq) and the mixture was stirred at 50° C. for anhour. The mixture was then diluted with methanol and condensed underreduced pressure to give a white powder (47.2 mg, 76.63% yield) that wascarried forward without further purification. ¹H NMR (400 MHz,Methanol-d4) δ 7.72 (ddd, J=8.5, 7.3, 5.0 Hz, 1H), 7.46-7.42 (m, 1H),7.30 (dd, J=8.6, 4.5 Hz, 1H), 4.94 (d, J=5.3 Hz, 2H), 2.81-2.56 (m, 2H),2.24-2.07 (m, 1H), 2.00 (s, 2H), 0.90 (t, J=6.5 Hz, 2H). LCMS 347 (M+H).

4,7-dichloro-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dione (D-51)

4,7-dichloroisobenzofuran-1,3-dione (434.6 mg, 2.002 mmol, 1 eq) wasadded to 3-aminopiperidine-2,6-dione hydrochloric acid (362.6 mg, 2.203mmol, 1.1 eq). Potassium acetate (609.07 mg, 6.206 mmol, 3.1 eq) andacetic acid (6.67 ml, 0.3 M) were then added to the mixture and it wasstirred at 90° C. for 18 hours. The mixture was cooled down to roomtemperature, diluted with DI water and centrifuged for 5 minutes. Theprecipitate was diluted with methanol and was condensed under reducedpressure. The crude material was purified by column chromatography(ISCO, 12 g silica column, 0 to 10% MeOH/DCM 25 minute gradient) to givea white powder (160.4 mg, 24.5% yield). ¹H NMR (500 MHz, DMSO-d₆) δ11.15 (s, 1H), 7.91 (s, 2H), 5.17 (dd, J=12.9, 5.4 Hz, 1H), 2.88 (ddd,J=17.2, 13.9, 5.4 Hz, 1H), 2.68-2.54 (m, 1H), 2.05 (ddd, J=10.5, 5.4,2.7 Hz, 1H). LCMS 328 (M+H).

Synthetic Example 1: Synthesis of dBET 1

(1) Synthesis of JQ-Acid

JQ1 (1.0 g, 2.19 mmol, 1 eq) was dissolved in formic acid (11 mL, 0.2 M)at room temperature and stirred for 75 hours. The mixture wasconcentrated under reduced pressure to give a yellow solid (0.99 g,quant yield) that was used without purification. ¹H NMR (400 MHz,Methanol-d₄) δ 7.50-7.36 (m, 4H), 4.59 (t, J=7.1 Hz, 1H), 3.51 (d, J=7.1Hz, 2H), 2.70 (s, 3H), 2.45 (s, 3H), 1.71 (s, 3H). LCMS 401.33 (M+H).

N-(4-aminobutyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetatewas synthesized according to the previously published procedure (Fischeret al., Nature 512 (2014):49).

(2) Synthesis of dBET1

JQ-acid (11.3 mg, 0.0281 mmol, 1 eq) andN-(4-aminobutyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate (14.5 mg, 0.0281 mmol, 1 eq) were dissolved in DMF(0.28 mL, 0.1 M) at room temperature. DIPEA (14.7 microliters, 0.0843mmol, 3 eq) and HATU (10.7 mg, 0.0281 mmol, 1 eq) were then added andthe mixture was stirred for 19 hours. The mixture was then purified bypreparative HPLC to give dBET1 as a yellow solid (15.90 mg, 0.0202 mmol,72%). ¹H NMR (400 MHz, Methanol-d₄) δ 7.77 (dd, J=8.3, 7.5 Hz, 1H), 7.49(d, J=7.3 Hz, 1H), 7.47-7.37 (m, 5H), 5.07 (dd, J=12.5, 5.4 Hz, 1H),4.74 (s, 2H), 4.69 (dd, J=8.7, 5.5 Hz, 1H), 3.43-3.32 (m, 3H), 3.29-3.25(m, 2H), 2.87-2.62 (m, 7H), 2.43 (s, 3H), 2.13-2.04 (m, 1H), 1.72-1.58(m, 7H). ¹³C NMR (100 MHz, cd₃od) δ 174.41, 172.33, 171.27, 171.25,169.87, 168.22, 167.76, 166.73, 166.70, 156.26, 138.40, 138.23, 137.44,134.83, 133.92, 133.40, 132.30, 132.28, 131.97, 131.50, 129.87, 121.85,119.31, 118.00, 69.53, 54.90, 50.54, 40.09, 39.83, 38.40, 32.12, 27.74,27.65, 23.61, 14.42, 12.97, 11.57. LCMS 785.44 (M+H).

Synthetic Example 2: Synthesis of dBET4

A 0.1 M solution ofN-(4-aminobutyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (0.438 mL, 0.0438 mmol 1.2 eq) was added to(R)-JQ-acid (prepared from (R)-JQ1 in an analogous method to JQ-acid)(14.63 mg, 0.0365 mmol, 1 eq) at room temperature. DIPEA (19.1microliters, 0.1095 mmol, 3 eq) and HATU (15.3 mg, 0.0402 mmol, 1.1 eq)were added and the mixture was stirred for 24 hours, then diluted withMeOH and concentrated under reduced pressure. The crude material waspurified by preparative HPLC to give a yellow solid (20.64 mg, 0.0263mmol, 72%). ¹H NMR (400 MHz, Methanol-d₄) δ 7.79 (dd, J=8.4, 7.4 Hz,1H), 7.51 (d, J=7.3 Hz, 1H), 7.47-7.39 (m, 5H), 5.11-5.06 (m, 1H), 4.75(s, 2H), 4.68 (dd, J=8.8, 5.5 Hz, 1H), 3.47-3.31 (m, 5H), 2.83-2.65 (m,7H), 2.44 (s, 3H), 2.13-2.06 (m, 1H), 1.68 (s, 3H), 1.67-1.60 (m, 4H).¹³C NMR (100 MHz, cd₃od) δ 174.43, 172.40, 171.29, 169.92, 168.24,167.82, 166.71, 156.31, 153.14, 138.38, 138.24, 137.54, 134.88, 133.86,133.44, 132.29, 132.00, 131.49, 129.88, 122.46, 121.90, 119.38, 118.02,69.59, 54.96, 50.55, 40.09, 39.84, 38.45, 32.14, 27.75, 27.65, 23.62,14.41, 12.96, 11.56. MS 785.48 (M+H).

Synthetic Example 3: Synthesis of dBET3

A 0.1 M solution ofN-(2-aminoethyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (0.475 mL, 0.0475 mmol, 1.2 eq) was added toJQ-acid (15.86 mg, 0.0396 mmol, 1 eq) at room temperature. DIPEA (20.7microliters, 0.1188 mmol, 3 eq) and HATU (16.5 mg, 0.0435 mmol, 1.1 eq)were then added and the mixture was stirred for 24 hours, then purifiedby preparative HPLC to give a yellow solid (22.14 mg, 0.0292 mmol, 74%).¹H NMR (400 MHz, Methanol-d₄) δ 7.82-7.75 (m, 1H), 7.52-7.32 (m, 6H),5.04 (dd, J=11.6, 5.5 Hz, 1H), 4.76 (d, J=3.2 Hz, 2H), 4.66 (d, J=6.6Hz, 1H), 3.58-3.35 (m, 6H), 2.78-2.58 (m, 6H), 2.48-2.41 (m, 3H),2.11-2.02 (m, 1H), 1.70 (d, J=11.8 Hz, 3H). ¹³C NMR (100 MHz, cd₃od) δ174.38, 171.26, 171.19, 170.26, 168.86, 168.21, 167.76, 166.72, 156.27,153.14, 138.44, 138.36, 138.19, 134.87, 133.71, 132.31, 131.57, 131.51,129.90, 129.86, 121.81, 119.36, 117.95, 69.48, 54.83, 50.52, 40.09,39.76, 38.30, 32.09, 23.63, 14.40, 11.61. LCMS 757.41 (M+H).

Synthetic Example 4: Synthesis of dBET5

A 0.1M solution ofN-(6-aminohexyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (0.247 mL, 0.0247 mmol, 1 eq) was added toJQ-acid (9.9 mg, 0.0247 mmol, 1 eq) at room temperature. DIPEA (12.9microliters, 0.0741 mmol, 3 eq) and HATU (9.4 mg, 0.0247 mmol, 1 eq)were then added. the mixture was stirred for 21 hours, then diluted withMeOH and concentrated under reduced pressure. The crude material waspurified by preparative HPLC to give a yellow solid (13.56 mg, 0.0167mmol, 67%). ¹H NMR (400 MHz, Methanol-d₄) δ 7.82-7.78 (m, 1H), 7.53 (dd,J=7.3, 2.0 Hz, 1H), 7.49-7.37 (m, 5H), 5.10 (dt, J=12.4, 5.3 Hz, 1H),4.76 (s, 2H), 4.70 (dd, J=8.7, 5.5 Hz, 1H), 3.42-3.33 (m, 2H), 3.25 (dt,J=12.3, 6.0 Hz, 3H), 2.87-2.67 (m, 7H), 2.48-2.42 (m, 3H), 2.14-2.09 (m,1H), 1.69 (d, J=4.8 Hz, 3H), 1.58 (s, 4H), 1.42 (d, J=5.2 Hz, 4H). ¹³CNMR (100 MHz, cd₃od) δ 174.51, 171.31, 171.26, 169.82, 168.27, 168.26,167.75, 156.26, 150.46, 138.20, 134.92, 133.92, 133.47, 132.34, 132.01,131.52, 129.88, 121.69, 119.34, 117.95, 111.42, 69.39, 54.97, 50.56,40.39, 40.00, 38.40, 32.15, 30.46, 30.16, 27.58, 27.48, 23.64, 14.41,12.96, 11.55. LCMS 813.38.

Synthetic Example 5: Synthesis of dBET6

A 0.1M solution ofN-(8-aminooctyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (0.191 mL, 0.0191 mmol, 1 eq) was added toJQ-acid (7.66 mg, 0.0191 mmol, 1 eq) at room temperature. DIPEA (10microliters, 0.0574 mmol, 3 eq) and HATU (7.3 mg, 0.0191 mmol, 1 eq)were added and the mixture was stirred for 22 hours, diluted with MeOH,and concentrated under reduced pressure. The crude material was purifiedby preparative HPLC to give a cream colored solid. (8.53 mg, 0.0101mmol, 53%). ¹H NMR (400 MHz, Methanol-d₄) δ 7.80 (dd, J=8.4, 7.4 Hz,1H), 7.53 (d, J=7.4 Hz, 1H), 7.49-7.36 (m, 5H), 5.10 (dt, J=12.3, 5.3Hz, 1H), 4.75 (s, 2H), 4.69 (dd, J=8.8, 5.3 Hz, 1H), 3.42 (dd, J=15.0,8.9 Hz, 1H), 3.30-3.18 (m, 4H), 2.90-2.64 (m, 7H), 2.45 (s, 3H), 2.13(dtt, J=10.8, 5.2, 2.6 Hz, 1H), 1.71 (d, J=4.4 Hz, 3H), 1.56 (d, J=6.2Hz, 4H), 1.33 (d, J=17.1 Hz, 8H). ¹³C NMR (100 MHz, cd₃od) δ 174.50,172.38, 171.30, 169.81, 168.28, 167.74, 166.64, 156.25, 138.38, 138.20,137.55, 134.92, 133.88, 133.42, 132.27, 132.02, 131.50, 129.85, 121.66,119.30, 117.95, 69.37, 55.01, 50.58, 40.51, 40.12, 38.44, 32.18, 30.46,30.33, 30.27, 30.21, 27.91, 27.81, 23.63, 14.42, 12.96, 11.55. LCMS841.64 (M+H).

Synthetic Example 6: Synthesis of dBET9

A 0.1M solution ofN-(3-(2-(2-(3-aminopropoxy)ethoxy)ethoxy)propyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (0.321 mL, 0.0321 mmol, 1 eq) was added toJQ-acid (12.87 mg, 0.0321 mmol, 1 eq) at room temperature. DIPEA (16.8microliters, 0.0963 mmol, 3 eq) and HATU (12.2 mg, 0.0321 mmol, 1 eq)were added and the mixture was stirred for 24 hours, diluted with MeOH,and concentrated under reduced pressure. The crude material was purifiedby preparative HPLC to give a yellow oil. (16.11 mg, 0.0176 mmol, 55%).

¹H NMR (400 MHz, Methanol-d₄) δ 7.79 (dd, J=8.4, 7.4 Hz, 1H), 7.52 (d,J=7.2 Hz, 1H), 7.49-7.36 (m, 5H), 5.10 (dd, J=12.5, 5.5 Hz, 1H),4.78-4.67 (m, 3H), 3.64-3.52 (m, 11H), 3.48-3.32 (m, 6H), 2.94-2.64 (m,7H), 2.52-2.43 (m, 3H), 2.18-2.08 (m, 1H), 1.81 (p, J=6.3 Hz, 4H),1.73-1.67 (m, 3H). LCMS 918.45 (M+H).

Synthetic Example 7: Synthesis of dBET17

A 0.1 M solution ofN-(4-aminobutyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (0.281 mL, 0.0281 mmol 1 eq) was added to(S)-2-(4-(4-cyanophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)aceticacid (11 mg, 0.0281 mmol, 1 eq) at room temperature. DIPEA (14.7microliters, 0.0843 mmol, 3 eq) and HATU (10.7 mg, 0.0281 mmol, 1 eq)were added and the mixture was stirred for 24 hours, diluted with EtOAcand washed with saturated sodium bicarbonate, water and brine. Theorganic layer was dried over sodium sulfate, filtered and condensed.Purification by column chromatography (ISCO, 4 g silica column 0-10%MeOH/DCM) gave a white solid (14.12 mg, 0.0182 mmol, 65%).

¹H NMR (400 MHz, Methanol-d₄) δ 7.82-7.72 (m, 3H), 7.61 (dd, J=8.5, 2.0Hz, 2H), 7.51 (d, J=7.9 Hz, 1H), 7.44-7.40 (m, 1H), 5.11-5.05 (m, 1H),4.76 (s, 2H), 4.66 (dd, J=9.0, 5.1 Hz, 1H), 3.48-3.32 (m, 4H), 3.30-3.23(m, 1H), 2.87-2.61 (m, 7H), 2.43 (s, 3H), 2.10 (dt, J=10.7, 5.2 Hz, 1H),1.70-1.59 (m, 7H). ¹³C NMR (100 MHz, cd₃od) δ 174.42, 172.65, 171.27,169.92, 168.25, 167.80, 165.88, 156.31, 143.55, 138.24, 134.88, 133.92,133.50, 133.39, 131.72, 131.46, 130.55, 121.93, 119.39, 119.21, 118.02,115.17, 69.59, 55.50, 50.55, 40.10, 39.83, 38.86, 32.11, 27.78, 27.67,23.62, 14.41, 12.91, 11.64. LCMS 776.39 (M+H).

Synthetic Example 8: Synthesis of dBET15

N-(6-aminohexyl)-2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindoline-5-carboxamidetrifluoroacetate (13.29 mg, 0.258 mmol, 1 eq) and JQ-acid (10.3 mg,0.0258 mmol, 1 eq) were dissolved in DMF (0.26 mL). DIPEA (13.5microliters, 0.0775 mmol, 3 eq) was added, followed by HATU (9.8 mg,0.0258 mmol, 1 eq) and the mixture was stirred at room temperature.After 24 hours, the material was diluted with DCM and purified by columnchromatography (ISCO, 0-15% MeOH/DCM) followed by preparative HPLC togive a pale yellow solid (11.44 mg, 0.0146 mmol 57%).

¹H NMR (400 MHz, Methanol-d₄) δ 8.29-8.23 (m, 2H), 7.93 (dd, J=8.1, 4.2Hz, 1H), 7.50-7.34 (m, 4H), 5.17-5.11 (m, 1H), 4.75-4.69 (m, 1H),3.53-3.32 (m, 6H), 3.25 (dd, J=13.8, 6.7 Hz, 1H), 2.90-2.67 (m, 6H),2.49-2.38 (m, 3H), 2.18-2.10 (m, 1H), 1.64 (d, J=22.4 Hz, 6H), 1.47 (s,4H). ¹³C NMR (100 MHz, cd₃od) δ 174.48, 171.17, 168.05, 168.03, 167.99,167.70, 166.63, 141.81, 138.40, 137.47, 135.09, 134.77, 134.74, 133.96,133.94, 133.38, 132.24, 132.05, 131.44, 129.85, 124.57, 123.12, 123.09,54.98, 50.78, 40.88, 40.08, 38.37, 32.13, 30.40, 30.23, 27.34, 27.26,23.58, 14.40, 12.96, 11.54. LCMS 783.43 (M+H).

Synthetic Example 9: Synthesis of dBET2

(1) Synthesis of (R)-ethyl4-((8-cyclopentyl-7-ethyl-5-methyl-6-oxo-5,6,7,8-tetrahydropteridin-2-yl)amino)-3-methoxybenzoate

(R)-2-chloro-8-cyclopentyl-7-ethyl-5-methyl-7,8-dihydropteridin-6(5H)-one(44.2 mg, 0.15 mmol, 1 eq), ethyl 4-amino-3-methoxybenzoate (35.1 mg,0.18 mmol, 1.2 eq), Pd₂dba₃ (6.9 mg, 0.0075 mmol, 5 mol %), XPhos (10.7mg, 0.0225 mmol, 15 mol %) and potassium carbonate (82.9 mg, 0.60 mmol,4 eq) were dissolved in tBuOH (1.5 mL, 0.1 M) and heated to 100° C.After 21 hours, the mixture was cooled to room temperature, filteredthrough celite, washed with DCM and concentrated under reduced pressure.Purification by column chromatography (ISCO, 4 g silica column, 0-100%EtOAc/hexanes over an 18 minute gradient) gave a yellow oil (52.3 mg,0.115 mmol, 77%). ¹H NMR (400 MHz, Chloroform-d) δ 8.57 (d, J=8.5 Hz,1H), 7.69 (td, J=6.2, 2.9 Hz, 2H), 7.54 (d, J=1.8 Hz, 1H), 4.52 (t,J=7.9 Hz, 1H), 4.37 (q, J=7.1 Hz, 2H), 4.23 (dd, J=7.9, 3.7 Hz, 1H),3.97 (s, 3H), 3.33 (s, 3H), 2.20-2.12 (m, 1H), 2.03-1.97 (m, 1H), 1.86(ddd, J=13.9, 7.6, 3.6 Hz, 4H), 1.78-1.65 (m, 4H), 1.40 (t, J=7.1 Hz,3H), 0.88 (t, J=7.5 Hz, 3H). LCMS 454.32 (M+H).

(2) Synthesis of(R)-4-((8-cyclopentyl-7-ethyl-5-methyl-6-oxo-5,6,7,8-tetrahydropteridin-2-yl)amino)-3-methoxybenzoicacid

(R)-ethyl4-((8-cyclopentyl-7-ethyl-5-methyl-6-oxo-5,6,7,8-tetrahydropteridin-2-yl)amino)-3-methoxybenzoate(73.8 mg, 0.163 mmol, 1 eq) and LiOH (11.7 mg, 0.489 mmol, 3 eq) weredissolved in MeOH (0.82 mL) THF (1.63 mL) and water (0.82 mL). After 20hours, an additional 0.82 mL of water was added and the mixture wasstirred for an additional 24 hours before being purified by preparativeHPLC to give a cream colored solid (53 mg, 0.125 mmol, 76%). H NMR (400MHz, Methanol-d₄) δ 7.97 (d, J=8.4 Hz, 1H), 7.67 (dd, J=8.3, 1.6 Hz,1H), 7.64-7.59 (m, 2H), 4.38 (dd, J=7.0, 3.2 Hz, 1H), 4.36-4.29 (m, 1H),3.94 (s, 3H), 3.30 (s, 3H), 2.13-1.98 (m, 2H), 1.95-1.87 (m, 2H),1.87-1.76 (m, 2H), 1.73-1.57 (m, 4H), 0.86 (t, J=7.5 Hz, 3H). ¹³C NMR(100 MHz, cd₃od)₆ 168.67, 163.72, 153.59, 150.74, 150.60, 130.95,127.88, 125.97, 123.14, 121.68, 116.75, 112.35, 61.76, 61.66, 56.31,29.40, 29.00, 28.68, 28.21, 23.57, 23.41, 8.69. LCMS 426.45 (M+H).

(3) Synthesis of dBET2

A 0.1 M solution ofN-(4-aminobutyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (0.183 mL, 0.0183 mmol 1.2 eq) was added to(R)-4-((8-cyclopentyl-7-ethyl-5-methyl-6-oxo-5,6,7,8-tetrahydropteridin-2-yl)amino)-3-methoxybenzoicacid (6.48 mg, 0.0152 mmol, 1 eq) at room temperature. DIPEA (7.9microliters, 0.0456 mmol, 3 eq) and HATU (6.4 mg, 0.0168 mmol, 1.1 eq)were added and the mixture was stirred for 23 hours, before beingpurified by preparative HPLC to give a yellow solid (9.44 mg, 0.0102mmol, 67%). ¹H NMR (400 MHz, Methanol-d₄) δ 7.84-7.77 (m, 2H), 7.58 (d,J=1.8 Hz, 2H), 7.53-7.46 (m, 2H), 7.42 (d, J=8.4 Hz, 1H), 5.11-5.05 (m,1H), 4.76 (s, 2H), 4.48 (dd, J=6.5, 3.1 Hz, 1H), 4.33-4.24 (m, 1H), 3.95(s, 3H), 3.49-3.35 (m, 4H), 2.97 (d, J=10.5 Hz, 3H), 2.89-2.65 (m, 5H),2.17-1.99 (m, 4H), 1.89 (dd, J=14.5, 7.3 Hz, 2H), 1.69-1.54 (m, 6H),1.36 (dt, J=7.6, 3.9 Hz, 1H), 0.85 (t, J=7.5 Hz, 3H). ¹³C NMR (100 MHz,cd₃od) δ 176.52, 174.48, 173.05, 171.34, 169.99, 168.91, 168.25, 167.80,164.58, 156.34, 154.48, 153.10, 150.63, 138.22, 134.89, 133.96, 129.53,123.93, 121.87, 120.78, 119.36, 117.99, 111.54, 69.55, 63.29, 63.10,56.68, 50.55, 40.71, 39.86, 32.15, 29.43, 29.26, 28.73, 28.63, 27.81,27.77, 24.25, 23.63, 8.47. LCMS 810.58 (M+H).

Synthetic Example 10: Synthesis of dBET7

A 0.1 M solutionN-(6-aminohexyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (0.186 mL, 0.0186 mmol 1 eq) was added to(R)-4-((8-cyclopentyl-7-ethyl-5-methyl-6-oxo-5,6,7,8-tetrahydropteridin-2-yl)amino)-3-methoxybenzoicacid (7.9 mg, 0.0186 mmol, 1 eq) at room temperature. DIPEA (9.7microliters, 0.0557 mmol, 3 eq) and HATU (7.1 mg, 0.0186 mmol, 1 eq)were added and the mixture was stirred for 19 hours, before beingpurified by preparative HPLC to give the desired trifluoracetate salt asa yellow solid(13.62 mg, 0.0143 mmol, 77%).

¹H NMR (400 MHz, Methanol-d₄) δ 7.80 (t, J=8.3 Hz, 2H), 7.61-7.57 (m,2H), 7.55-7.49 (m, 2H), 7.42 (d, J=8.4 Hz, 1H), 5.13 (dd, J=12.6, 5.5Hz, 1H), 4.75 (s, 2H), 4.48 (dd, J=6.5, 3.2 Hz, 1H), 4.33-4.24 (m, 1H),3.97 (s, 3H), 3.40 (t, J=7.1 Hz, 2H), 3.34 (d, J=6.7 Hz, 2H), 3.30 (s,3H), 2.98 (d, J=8.5 Hz, 1H), 2.89-2.82 (m, 1H), 2.79-2.63 (m, 3H),2.17-2.00 (m, 4H), 1.91 (dt, J=14.4, 7.1 Hz, 3H), 1.61 (dt, J=13.4, 6.6Hz, 7H), 1.47-1.41 (m, 3H), 0.86 (t, J=7.5 Hz, 3H). ¹³C NMR (100 MHz,cd₃od) δ 174.54, 171.37, 169.84, 168.84, 168.27, 167.74, 164.59, 156.26,154.47, 153.18, 150.69, 138.19, 134.91, 134.05, 129.47, 124.78, 124.01,121.65, 120.77, 119.29, 117.92, 117.86, 111.55, 69.34, 63.31, 63.13,56.67, 50.53, 40.97, 39.96, 32.16, 30.42, 30.19, 29.42, 29.26, 28.72,28.62, 27.65, 27.46, 24.26, 23.65, 8.47. LCMS 838.60 (M+H).

Synthetic Example 11: Synthesis of dBET8

A 0.1 M solutionN-(8-aminooctyl)-2-((2-(2,6-dioxopiperdin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (0.186 mL, 0.0186 mmol 1 eq) was added to(R)-4-((8-cyclopentyl-7-ethyl-5-methyl-6-oxo-5,6,7,8-tetrahydropteridin-2-yl)amino)-3-methoxybenzoicacid (7.9 mg, 0.0186 mmol, 1 eq) at room temperature. DIPEA (9.7microliters, 0.0557 mmol, 3 eq) and HATU (7.1 mg, 0.0186 mmol, 1 eq)were added and the mixture was stirred for 16 hours, before beingpurified by preparative HPLC to give the desired trifluoracetate salt asan off-white solid(7.15 mg, 0.007296 mmol, 39%).

¹H NMR (400 MHz, Methanol-d₄) δ 7.83-7.77 (m, 2H), 7.61-7.56 (m, 2H),7.55-7.50 (m, 2H), 7.42 (d, J=8.5 Hz, 1H), 5.13 (dd, J=12.6, 5.5 Hz,1H), 4.75 (s, 2H), 4.49 (dd, J=6.6, 3.3 Hz, 1H), 4.33-4.24 (m, 1H), 3.97(s, 3H), 3.39 (t, J=7.1 Hz, 2H), 3.34-3.32 (m, 2H), 3.30 (s, 3H),3.01-2.83 (m, 2H), 2.82-2.65 (m, 3H), 2.17-2.01 (m, 4H), 1.91 (dt,J=14.2, 7.4 Hz, 1H), 1.68-1.54 (m, 7H), 1.37 (s, 7H), 0.86 (t, J=7.5 Hz,3H). ¹³C NMR (100 MHz, cd₃od) δ 174.52, 171.35, 169.81, 168.85, 168.28,167.74, 164.58, 156.27, 154.47, 153.89, 150.64, 138.19, 134.93, 134.18,129.52, 129.41, 124.91, 123.83, 121.67, 120.76, 119.31, 117.95, 117.89,111.57, 69.37, 63.37, 63.17, 56.67, 50.58, 41.12, 40.12, 32.19, 30.43,30.28, 30.22, 30.19, 29.40, 29.25, 28.71, 28.62, 27.94, 27.75, 24.29,23.65, 8.46. LCMS 866.56 (M+H).

Synthetic Example 12: Synthesis of dBET10

A 0.1 M solutionN-(3-(2-(2-(3-aminopropoxy)ethoxy)ethoxy)propyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (0.172 mL, 0.0172 mmol 1 eq) was added to(R)-4-((8-cyclopentyl-7-ethyl-5-methyl-6-oxo-5,6,7,8-tetrahydropteridin-2-yl)amino)-3-methoxybenzoicacid (7.3 mig, 0.0172 mmol, 1 eq) at room temperature. DIPEA (9.0microliters, 0.0515 mmol, 3 eq) and HATU (6.5 mg, 0.0172 mmol, 1 eq)were added and the mixture was stirred for 23 hours, before beingpurified by preparative HPLC to give the desired trifluoracetate salt asan off-white oil (10.7 mg, 0.0101 mmol, 59%).

¹H NMR (400 MHz, Methanol-d₄) δ 7.78 (d, J=8.3 Hz, 1H), 7.75 (dd, J=8.4,7.4 Hz, 1H), 7.56-7.51 (m, 2H), 7.49-7.44 (m, 2H), 7.36 (d, J=8.4 Hz,1H), 5.08 (dd, J=12.4, 5.4 Hz, 1H), 4.69 (s, 2H), 4.44 (dd, J=6.7, 3.2Hz, 1H), 4.30-4.21 (m, 1H), 3.92 (s, 3H), 3.59-3.42 (m, 12H), 3.35 (t,J=6.7 Hz, 2H), 3.25 (s, 3H), 2.95-2.64 (m, 5H), 2.13-1.95 (m, 4H),1.91-1.71 (m, 7H), 1.65-1.48 (m, 4H), 0.81 (t, J=7.5 Hz, 3H). ¹³C NMR(100 MHz, cd₃od) δ 174.50, 171.35, 169.83, 168.77, 168.25, 167.68,164.57, 156.26, 154.47, 153.05, 150.59, 138.19, 134.92, 133.89, 129.53,124.57, 123.98, 121.72, 120.75, 119.26, 117.95, 117.86, 111.54, 71.51,71.46, 71.28, 71.20, 70.18, 69.65, 69.41, 63.27, 63.07, 56.71, 50.57,38.84, 37.59, 32.17, 30.41, 30.32, 29.46, 29.26, 28.73, 28.64, 24.27,23.65, 8.49. LCMS 942.62 (M+H).

Synthetic Example 13: Synthesis of dBET16

A 0.1 M solution ofN-(4-aminobutyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (0.402 mL, 0.0402 mmol 1 eq) was added(R)-4-((4-cyclopentyl-1,3-dimethyl-2-oxo-1,2,3,4-tetrahydropyrido[2,3-b]pyrazin-6-yl)amino)-3-methoxybenzoicacid (16.55 mg, 0.0402 mmol, 1 eq) at room temperature. DIPEA (21microliters, 0.1206 mmol, 3 eq) and HATU (15.3 mg, 0.0402 mmol, 1 eq)were added and the mixture was stirred for 21 hours, before beingpurified by preparative HPLC, followed by column chromatography (ISCO,12 g NH₂-silica column, 0-15% MeOH/DCM, 20 min gradient) to give HPLC togive a brown solid (10.63 mg, 0.0134 mmol, 33%).

¹H NMR (400 MHz, Methanol-d₄) δ 8.22 (d, J=8.4 Hz, 1H), 7.78 (dd, J=8.4,7.4 Hz, 1H), 7.73 7.68 (m, 1H), 7.49 (d, J=7.4 Hz, 2H), 7.46-7.39 (m,2H), 6.98 (d, J=8.8 Hz, 1H), 5.97-5.87 (m, 1H), 5.06 (dd, J=12.6, 5.4Hz, 1H), 4.76 (s, 2H), 3.98 (s, 3H), 3.61 (s, 2H), 3.44-3.36 (m, 4H),2.92 (s, 1H), 2.78 (dd, J=14.3, 5.2 Hz, 1H), 2.68 (ddd, J=17.7, 8.2, 4.5Hz, 2H), 2.36-2.26 (m, 2H), 2.10-1.90 (m, 5H), 1.76-1.62 (m, 6H), 1.31(d, J=16.0 Hz, 4H). LCMS 795.38 (M+H).

Synthetic Example 14: Synthesis of dBET 11

(1) Synthesis of ethyl4-((5,11-dimethyl-6-oxo-6,11-dihydro-5H-benzo[e]pyrimido[5,4-b][1,4]diazepin-2-yl)amino)-3-methoxybenzoate

2-chloro-5,11-dimethyl-5H-benzo[e]pyrimido[5,4-b][1,4]diazepin-6(11H)-one(82.4mg, 0.30 mmol, 1 eq), ethyl 4-amino-3-methoxybenzoate (70.3 mg, 0.36mmol, 1.2 eq) Pd₂dba₃ (13.7 mg, 0.015 mmol, 5 mol %), XPhos (21.5 mg,0.045 mmol, 15 mol %) and potassium carbonate (166 mg, 1.2 mmol, 4 eq)were dissolved in tBuOH (3.0 mL) and heated to 100° C. After 17 hours,the mixture was cooled room temperature and filtered through celite. Themixture was purified by column chromatography (ISCO, 12 g silica column,0-100% EtOAc/hexanes, 19 min gradient) to give an off white solid (64.3mg, 0.148 mmol, 49%).

¹H NMR (400 MHz, 50% cd₃od/cdcl₃) δ 8.51 (d, J=8.5 Hz, 1H), 8.17 (s,1H), 7.73 (ddd, J=18.7, 8.1, 1.7 Hz, 2H), 7.52 (d, J=1.8 Hz, 1H),7.46-7.41 (m, 1H), 7.15-7.10 (m, 2H), 4.34 (q, J=7.1 Hz, 4H), 3.95 (s,3H), 3.47 (s, 3H), 3.43 (s, 3H), 1.38 (t, J=7.1 Hz, 3H). ¹³C NMR (100MHz, 50% cd₃od/cdcl₃) δ 169.28, 167.39, 164.29, 155.64, 151.75, 149.73,147.45, 146.22, 133.88, 133.18, 132.37, 126.44, 124.29, 123.70, 123.36,122.26, 120.58, 118.05, 116.83, 110.82, 61.34, 56.20, 38.62, 36.25,14.51. LCMS 434.33 (M+H).

(2) Synthesis of4-((5,11-dimethyl-6-oxo-6,11-dihydro-5H-benzo[e]pyrimido[5,4-b][1,4]diazepin-2-yl)amino)-3-methoxybenzoicacid

Ethyl4-((5,11-dimethyl-6-oxo-6,11-dihydro-5H-benzo[e]pyrimido[5,4-b][1,4]diazepin-2-yl)amino)-3-methoxybenzoate(108.9 mg, 0.251 mmol, 1 eq) and LiOH (18 mg) were dissolved in THF (2.5mL) and water (1.25 mL). After 24 hours, MeOH (0.63 mL) was added toimproved solubility) and stirred for an additional 24 hours before beingdiluted with MeOH and purified by preparative HPLC to give a lightyellow solid (41.31 mg).

¹H NMR (400 MHz, Methanol-d₄) δ 8.51 (d, J=8.5 Hz, 1H), 8.22 (s, 1H),7.73 (ddd, J=11.8, 8.1, 1.7 Hz, 2H), 7.57 (d, J=1.8 Hz, 1H), 7.49-7.44(m, 1H), 7.19-7.11 (m, 2H), 3.97 (s, 3H), 3.48 (s, 3H), 3.45 (s, 3H).LCMS 406.32 (M+H).

(3) Synthesis of dBET11

A 0.1 M solution ofN-(4-aminobutyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (0.190 mL, 0.0190 mmol 1 eq) was added to4-((5,11-dimethyl-6-oxo-6,11-dihydro-5H-benzo[e]pyrimido[5,4-b][1,4]diazepin-2-yl)amino)-3-methoxybenzoicacid(7.71 mg, 0.0190 mmol, 1 eq) at room temperature. DIPEA (9.9microliters, 0.0571 mmol, 3 eq) and HATU (7.2 mg, 0.0190 mmol, 1 eq)were added and the mixture was stirred for 22 hours, before beingpurified by preparative HPLC to give HPLC to give the desiredtrifluoracetate salt as a cream colored solid (6.72 mg, 0.00744 mmol,39%).

¹H NMR (400 MHz, Methanol-d₄) δ 8.46 (d, J=8.3 Hz, 1H), 8.21 (s, 1H),7.79-7.73 (m, 2H), 7.52 (d, J=7.1 Hz, 1H), 7.50-7.43 (m, 3H), 7.33 (d,J=8.2 Hz, 1H), 7.15 (dd, J=7.7, 5.9 Hz, 2H), 4.98 (dd, J=12.0, 5.5 Hz,1H), 4.69 (s, 2H), 3.97 (s, 3H), 3.49 (s, 3H), 3.46-3.34 (m, 7H),2.81-2.67 (m, 3H), 2.13-2.08 (m, 1H), 1.69 (dt, J=6.6, 3.5 Hz, 4H). ¹³CNMR (100 MHz, cd₃od) δ 173.40, 170.10, 169.68, 169.00, 168.85, 167.60,167.15, 164.77, 156.01, 155.42, 151.83, 150.03, 148.21, 137.82, 134.12,133.48, 132.58, 132.52, 128.11, 126.72, 124.54, 122.33, 121.06, 120.63,118.77, 118.38, 117.94, 117.62, 109.67, 68.90, 56.33, 49.96, 40.16,39.48, 38.72, 36.34, 31.82, 27.24, 23.16. LCMS 790.48 (M+H).

Synthetic Example 15: Synthesis of dBET12

A 0.1 M solutionN-(3-(2-(2-(3-aminopropoxy)ethoxy)ethoxy)propyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (0.186 mL, 0.0186 mmol 1 eq) was added to4-((5,11-dimethyl-6-oxo-6,11-dihydro-5H-benzo[e]pyrimido[5,4-b][1,4]diazepin-2-yl)amino)-3-methoxybenzoicacid(7.53 mg, 0.0186 mmol, 1 eq) at room temperature. DIPEA (9.7microliters, 0.0557 mmol, 3 eq) and HATU (7.1 mg, 0.0186 mmol, 1 eq)were added and the mixture was stirred for 22 hours, before beingpurified by preparative HPLC to give HPLC to give the desiredtrifluoracetate salt as a cream colored solid (7.50 mg, 0.00724 mmol,39%).

¹H NMR (400 MHz, Methanol-d₄) δ 8.46 (d, J=8.9 Hz, 1H), 8.21 (s, 1H),7.73 (dd, J=15.2, 7.8 Hz, 2H), 7.50-7.42 (m, 3H), 7.28 (d, J=8.5 Hz,1H), 7.15 (t, J=7.7 Hz, 2H), 5.01 (dd, J=11.8, 5.8 Hz, 1H), 4.68 (s,2H), 3.97 (s, 3H), 3.67-3.58 (m, 7H), 3.58-3.43 (m, 10H), 3.39 (t, J=6.8Hz, 2H), 3.35 (s, 2H), 2.97 (s, 1H), 2.84-2.70 (m, 3H), 2.16-2.07 (m,1H), 1.93-1.76 (m, 4H). LCMS 922.57 (M+H).

Synthetic Example 16: Synthesis of dBET13

A 0.1 M solution ofN-(4-aminobutyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (0.501 mL, 0.0501 mmol 1 eq) was added to2-((2-(4-(3,5-dimethylisoxazol-4-yl)phenyl)imidazo[1,2-a]pyrazin-3-yl)amino)aceticacid (synthesized as in McKeown et al, J. Med. Chem, 2014, 57, 9019)(18.22 mg, 0.0501 mmol, 1 eq) at room temperature. DIPEA (26.3microliters, 0.150 mmol, 3 eq) and HATU (19.0 mg, 0.0501 mmol, 1 eq)were added and the mixture was stirred for 21 hours, before beingpurified by preparative HPLC to give HPLC to give the desiredtrifluoracetate salt as a dark yellow oil (29.66 mg, 0.0344 mmol, 69%).¹H NMR (400 MHz, Methanol-d₄) δ 9.09 (s, 1H), 8.65 (d, J=5.2 Hz, 1H),8.14-8.06 (m, 2H), 7.94-7.88 (m, 1H), 7.80-7.74 (m, 1H), 7.59-7.47 (m,3H), 7.40 (dd, J=8.4, 4.7 Hz, 1H), 5.11-5.06 (m, 1H), 4.72 (d, J=9.8 Hz,2H), 3.90 (s, 2H), 3.25-3.22 (m, 1H), 3.12 (t, J=6.4 Hz, 1H), 2.96 (s,2H), 2.89-2.79 (m, 1H), 2.76-2.62 (m, 2H), 2.48-2.42 (m, 3H), 2.29 (s,3H), 2.10 (ddq, J=10.2, 5.3, 2.7 Hz, 1H), 1.49-1.45 (m, 2H), 1.37 (dd,J=6.7, 3.6 Hz, 2H). 13C NMR (100 MHz, cd₃od) δ 174.45, 171.98, 171.35,169.88, 168.17, 167.85, 167.40, 159.88, 156.28, 141.82, 138.26, 135.85,134.82, 133.09, 132.06, 130.75, 129.67, 122.07, 121.94, 119.30, 118.98,118.06, 117.24, 69.56, 50.56, 40.05, 39.73, 32.13, 27.53, 23.62, 18.71,17.28, 11.64, 10.85. LCMS 748.49 (M+H).

Synthetic Example 17: Synthesis of dBET14

A 0.1 M solutionN-(3-(2-(2-(3-aminopropoxy)ethoxy)ethoxy)propyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (0.510 mL, 0.0510 mmol 1 eq) was added to2-((2-(4-(3,5-dimethylisoxazol-4-yl)phenyl)imidazo[1,2-a]pyrazin-3-yl)amino)aceticacid (synthesized as in McKeown et al, J. Med. Chem, 2014, 57, 9019)(18.52 mg, 0.0510 mmol, 1 eq) at room temperature. DIPEA (26.6microliters, 0.153 mmol, 3 eq) and HATU (19.4 mg, 0.0510 mmol, 1 eq)were added and the mixture was stirred for 22 hours, before beingpurified by preparative HPLC to give HPLC to give the desiredtrifluoracetate salt as a dark yellow oil (32.63 mg, 0.0328 mmol, 64%).

¹H NMR (400 MHz, Methanol-d₄) δ 9.09 (s, 1H), 8.66 (d, J=5.4 Hz, 1H),8.17-8.08 (m, 2H), 7.92 (d, J=5.6 Hz, 1H), 7.77 (dd, J=8.4, 7.4 Hz, 1H),7.60-7.47 (m, 3H), 7.39 (d, J=8.4 Hz, 1H), 5.09 (dd, J=12.4, 5.5 Hz,1H), 4.71 (s, 2H), 3.91 (s, 2H), 3.62-3.46 (m, 10H), 3.38 (dt, J=16.0,6.4 Hz, 3H), 3.18 (t, J=6.8 Hz, 2H), 2.97 (s, 1H), 2.89-2.81 (m, 1H),2.78-2.66 (m, 2H), 2.47 (s, 3H), 2.31 (s, 3H), 2.16-2.08 (m, 1H), 1.79(dt, J=12.8, 6.5 Hz, 2H), 1.64 (t, J=6.3 Hz, 2H). ¹³C NMR (100 MHz,cd₃od) δ 174.48, 171.88, 171.34, 169.80, 168.22, 167.69, 167.42, 159.87,156.24, 141.87, 138.21, 135.89, 134.88, 133.13, 132.04, 130.76, 129.67,122.08, 121.69, 119.20, 117.94, 117.23, 71.44, 71.22, 71.10, 69.92,69.62, 69.38, 50.57, 49.64, 38.11, 37.55, 32.16, 30.30, 30.20, 23.63,11.67, 10.88. LCMS 880.46 (M+H).

Synthetic Example 18: Synthesis of dBET 18

(1) Synthesis of (S)-tert-butyl4-(3-(2-(4-(4-chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)acetamido)propyl)piperazine-1-carboxylate

JQ-acid (176.6 mg, 0.441 mmol, 1 eq) was dissolved in DMF (4.4 mL) atroom temperature. HATU (176 mg, 0.463 mmol, 1.05 eq) was added, followedby DIPEA (0.23 mL), 1.32 mmol, 3 eq). After 10 minutes, tert-butyl4-(3-aminopropyl)piperazine-1-carboxylate (118 mg, 0.485 mmol, 1.1 eq)was added as a solution in DMF (0.44 mL). After 24 hours, the mixturewas diluted with half saturated sodium bicarbonate and extracted twicewith DCM and once with EtOAc. The combined organic layer was dried oversodium sulfate, filtered and condensed. Purification by columnchromatography (ISCO, 24 g silica column, 0-15% MeOH/DCM, 23 minutegradient) gave a yellow oil (325.5 mg, quant yield)

¹H NMR (400 MHz, Chloroform-d) δ 7.67 (t, J=5.3 Hz, 1H), 7.41-7.28 (m,4H), 4.58 (dd, J=7.5, 5.9 Hz, 1H), 3.52-3.23 (m, 8H), 2.63 (s, 9H), 2.37(s, 3H), 1.80-1.69 (m, 2H), 1.64 (s, 3H), 1.42 (s, 9H). ¹³C NMR (100MHz, cdcl₃) δ 171.41, 164.35, 155.62, 154.45, 150.20, 136.92, 136.64,132.19, 131.14, 130.98, 130.42, 129.98, 128.80, 80.24, 56.11, 54.32,52.70, 38.96, 37.85, 28.42, 25.17, 14.43, 13.16, 11.82. LCMS 626.36(M+H).

(2) Synthesis of(S)-2-(4-(4-chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-N-(3-(piperazin-1-yl)propyl)acetamide

(S)-tert-butyl4-(3-(2-(4-(4-chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)acetamido)propyl)piperazine-1-carboxylate(325.5 mg) was dissolved in DCM (5 mL) and MeOH (0.5 mL). A solution of4M HCl in dioxane (1 mL) was added and the mixture was stirred for 16hours, then concentrated under a stream of nitrogen to give a yellowsolid (231.8 mg) which was used without further purification.

¹H NMR (400 MHz, Methanol-d₄) δ 7.64-7.53 (m, 4H), 5.05 (t, J=7.1 Hz,1H), 3.81-3.66 (m, 6H), 3.62-3.33 (m, 9H), 3.30 (p, J=1.6 Hz, 1H), 2.94(s, 3H), 2.51 (s, 3H), 2.09 (dq, J=11.8, 6.1 Hz, 2H), 1.72 (s, 3H). ¹³CNMR (100 MHz, cd₃od) δ 171.78, 169.38, 155.83, 154.03, 152.14, 140.55,136.33, 134.58, 134.53, 133.33, 132.73, 130.89, 130.38, 56.07, 53.54,41.96, 37.22, 36.23, 25.11, 14.48, 13.14, 11.68. LCMS 526.29 (M+H).

(3) Synthesis of (S)-tert-butyl(6-(4-(3-(2-(4-(4-chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)acetamido)propyl)piperazin-1-yl)-6-oxohexyl)carbamate

(S)-2-(4-(4-chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-J][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-N-(3-(piperazin-1-yl)propyl)acetamide(62.1 mg) and 6-((tert-butoxycarbonyl)amino)hexanoic acid (24.0 mg,0.1037 mmol, 1 eq) were dissolved in DMF (1 mL). DIPEA (72.2microliters, 0.4147 mmol, 4 eq) was added, followed by HATU (39.4 mg,0.1037 mmol, 1 eq) and the mixture was stirred for 25 hours. The mixturewas diluted with half saturated sodium bicarbonate and extracted threetimes with DCM. The combined organic layer was dried over sodiumsulfate, filtered and condensed. Purification by column chromatography(ISCO, 4 g silica column, 0-15% MeOH/DCM, 15 minute gradient) gave ayellow oil (71.75 mg, 0.0970 mmol, 94%).

¹H NMR (400 MHz, Chloroform-d) δ 7.61 (s, 1H), 7.43-7.28 (m, 4H), 4.63(s, 1H), 4.61-4.56 (m, 1H), 3.82-3.21 (m, 10H), 3.11-3.01 (m, 2H), 2.61(d, J=24.3 Hz, 9H), 2.38 (s, 3H), 2.28 (t, J=7.4 Hz, 2H), 1.73 (dq,J=13.8, 7.4 Hz, 2H), 1.63-1.55 (m, 2H), 1.53-1.24 (m, 14H). ¹³C NMR (100MHz, cdcl₃)₆ 171.63, 171.11, 164.34, 156.17, 155.66, 150.21, 136.96,136.72, 132.25, 131.14, 131.01, 130.47, 130.00, 128.85, 79.11, 56.42,54.46, 53.06, 52.82, 45.04, 41.02, 40.47, 39.29, 38.33, 33.00, 29.90,28.54, 26.60, 25.29, 24.86, 14.47, 13.20, 11.86. LCMS 739.37 (M+H).

(4) Synthesis of(S)—N-(3-(4-(6-aminohexanoyl)piperazin-1-yl)propyl)-2-(4-(4-chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)acetamide

(S)-tert-butyl(6-(4-(3-(2-(4-(4-chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-J][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)acetamido)propyl)piperazin-1-yl)-6-oxohexyl)carbamate(71.75 mg, 0.0970 mmol, 1 eq) was dissolved in DCM (2 mL) and MeOH (0.2mL). A solution of 4M HCl in dioxane (0.49 mL) was added and the mixturewas stirred for 2 hours, then concentrated under a stream of nitrogen,followed by vacuum to give a yellow foam (59.8 mg, 0.0840 mmol, 87%).

¹H NMR (400 MHz, Methanol-d₄) δ 7.68-7.53 (m, 4H), 5.04 (d, J=6.6 Hz,1H), 4.66 (d, J=13.6 Hz, 1H), 4.23 (d, J=13.6 Hz, 1H), 3.63-3.34 (m,7H), 3.29-3.00 (m, 5H), 2.95 (d, J=6.0 Hz, 5H), 2.51 (d, J=9.2 Hz, 5H),2.08 (s, 2H), 1.77-1.62 (m, 7H), 1.45 (dt, J=15.3, 8.6 Hz, 2H). ¹³C NMR(100 MHz, cd₃od) δ 173.77, 171.84, 169.35, 155.85, 153.99, 140.56,136.40, 134.58, 133.35, 132.70, 130.39, 55.83, 53.57, 52.92, 52.70,43.57, 40.55, 39.67, 37.33, 36.25, 33.17, 28.26, 26.94, 25.33, 25.26,14.49, 13.15, 11.65. LCMS 639.35 (M+H).

(5) Synthesis of dBET18

(S)—N-(3-(4-(6-aminohexanoyl)piperazin-1-yl)propyl)-2-(4-(4-chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)acetamidedihydrochloride (20.0 mg, 0.0281 mmol, 1 eq) and2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetic acid(9.32 mg, 0.0281 mmol, 1 eq) were dissolved in DMF (0.281 mL). DIPEA(19.6 microliters, 0.1124 mmol, 4 eq) was added, followed by HATU (10.7mg, 0.0281 mmol, 1 eq). After 24 hours, the mixture was diluted withMeOH and purified by preparative HPLC to give the desiredtrifluoracetate salt.

¹H NMR (400 MHz, Methanol-d₄) δ 7.83-7.79 (m, 1H), 7.54 (d, J=7.1 Hz,1H), 7.45 (q, J=8.8 Hz, 5H), 5.12 (dd, J=12.5, 5.4 Hz, 1H), 4.76 (s,2H), 4.68 (t, J=7.3 Hz, 1H), 3.59-3.32 (m, 8H), 3.28-3.18 (m, 4H), 2.87(ddd, J=19.0, 14.7, 5.3 Hz, 2H), 2.80-2.65 (m, 6H), 2.44 (d, J=6.8 Hz,5H), 2.33-2.25 (m, 1H), 2.14 (dd, J=9.8, 4.9 Hz, 1H), 2.06-1.89 (m, 3H),1.70 (s, 3H), 1.61 (dq, J=14.4, 7.3, 6.9 Hz, 4H), 1.45-1.37 (m, 2H). ¹³CNMR (100 MHz, cd₃od) δ 174.52, 173.97, 173.69, 171.44, 169.88, 168.26,167.83, 166.72, 156.36, 138.28, 137.84, 134.89, 133.52, 132.12, 131.83,131.38, 129.89, 121.87, 119.32, 118.01, 69.52, 55.64, 55.03, 52.79,50.58, 43.69, 39.77, 38.57, 36.89, 33.47, 32.16, 29.93, 27.34, 25.76,25.45, 23.63, 14.39, 12.94, 11.66. LCMS 953.43 (M+H).

Synthetic Example 19: Synthesis of dBET19

A 0.1 M solution ofN-(4-aminobutyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (235 microliters, 0.023 5 mmol, 1 eq) was addedto(S)-2-(4-(4-chlorophenyl)-2-(cyanomethyl)-3,9-dimethyl-6H-thieno[3,2-J][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)aceticacid (10 mg, 0.0235 mmol, 1 eq) at room temperature. DIPEA (12.3microliters, 0.0704 mmol, 3 eq) and HATU (8.9 μg, 0.0235 mmol, 1 eq)were added and the mixture was stirred for 18.5 hours. The mixture wasthen diluted with EtOAc and washed with saturated sodium bicarbonate,water and brine. The organic layer was dried over sodium sulfate,filtered and concentrated under reduced pressure. Purification by columnchromatography (ISCO, 4 g silica column, 0-10% MeOH/DCM, 25 minutegradient) gave the desired product as a white solid (12.96 mg, 0.0160mmol, 68%). ¹H NMR (400 MHz, Chloroform-d) δ 7.80 (dd, J=8.4, 7.4 Hz,1H), 7.55-7.37 (m, 6H), 5.14-5.06 (m, 1H), 4.77 (d, J=1.5 Hz, 2H), 4.64(dd, J=8.0, 5.6 Hz, 1H), 3.45-3.32 (m, 5H), 3.29-3.21 (m, 2H), 2.83 2.66(m, 6H), 2.58 (s, 3H), 2.14-2.06 (m, 1H), 1.71-1.57 (m, 4H). LCMS810.30, M+H).

Synthetic Example 20: Synthesis of dBET20

3-((2-((4-(4-(4-aminobutanoyl)piperazin-1-yl)phenyl)amino)-5-methylpyrimidin-4-yl)amino)-N-(tert-butyl)benzenesulfonamidetrifluoroacetate (7.41 mg, 0.0107 mmol, 1 eq) and2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetic acid(3.6 mg, 0.0107 mmol, 1 eq) were dissolved in DMF (214 microliters,0.05M) at room temperature. DIPEA (5.6 microliters, 0.0321 mmol, 3 eq)and HATU (4.1 mg, 0.0107 mmol, 1 eq) were added. After 22.5 hours, themixture was diluted with MeOH and purified by preparative HPLC to givethe desired product as a brown residue (6.27 mg, 0.00701 mmol, 65%). ¹HNMR (500 MHz, Methanol-d₄) δ 8.06 (s, 1H), 7.84-7.75 (m, 3H), 7.65 (s,1H), 7.55 (t, J=7.8 Hz, 2H), 7.45 (d, J=8.4 Hz, 1H), 7.25-7.20 (m, 2H),6.99 (d, J=8.8 Hz, 2H), 5.11 (dd, J=12.5, 5.4 Hz, 1H), 4.78 (s, 2H),3.79-3.66 (m, 4H), 3.40 (t, J=6.6 Hz, 2H), 3.24-3.13 (m, 4H), 2.82-2.68(m, 3H), 2.52 (t, J=7.4 Hz, 2H), 2.24-2.19 (m, 3H), 2.12 (dd, J=10.2,5.1 Hz, 1H), 1.92 (dd, J=13.4, 6.4 Hz, 2H), 1.18 (s, 9H). LCMS 895.63(M+H).

Synthetic Example 21: Synthesis of dBET21

A 0.1 M solution of4-((10-aminodecyl)oxy)-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dionetrifluoroacetate in DMF (232 microliters, 0.0232 mmol, 1 eq) was addedto JQ-acid (9.3 mg, 0.0232 mmol, 1 eq) at room temperature. DIPEA (12.1microliters, 0.0696 mmol, 3 eq) and HATU (8.8 mg, 0.0232 mmol, 1 eq)were added and the mixture was stirred for 18 hours. The mixture wasthen diluted with EtOAc and washed with saturated sodium bicarbonate,water and brine. The organic layer was dried over sodium sulfate,filtered and concentrated under reduced pressure. Purification bypreparative HPLC followed by column chromatography (ISCO, 4 g silicacolumn, 0-10% MeOH/DCM, 25 minute gradient) gave the desired product asan off-white residue (1.84 mg, 0.00235 mmol, 10%). ¹H NMR (500 MHz,Methanol-d₄) δ 7.77-7.73 (m, 1H), 7.50-7.33 (m, 6H), 5.09 (dd, J=12.5,5.5 Hz, 1H), 4.62 (s, 1H), 4.21 (t, J=6.4 Hz, 2H), 3.36 (s, 2H),2.87-2.67 (m, 6H), 2.44 (s, 3H), 1.88-1.82 (m, 2H), 1.70 (s, 3H), 1.58(s, 4H), 1.29 (s, 8H). LCMS 784.51 (M+H).

Synthetic Example 22: Synthesis of dBET22

A 0.1 M solution ofN-(4-aminobutyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (247 microliters, 0.0247 mmol, 1 eq) was addedto(S)-4-(4-chlorophenyl)-6-(2-methoxy-2-oxoethyl)-3,9-dimethyl-6H-thieno[3,2-J][1,2,4]triazolo[4,3-a][1,4]diazepine-2-carboxylicacid (10.98 mg, 0.0247 mmol, 1 eq) at room temperature. DIPEA (12.9microliters, 0.0740 mmol, 3 eq) and HATU (9.4 mg, 0.0247 mmol, 1 eq)were added. The mixture was then stirred for 21 hours, then diluted withEtOAc and washed with saturated sodium bicarbonate, water and brine. Theorganic layer was dried over sodium sulfate, filtered and concentratedunder reduced pressure. Purification by column chromatography (ISCO, 4 gsilica column, 0-10% MeOH/DCM, 25 minute gradient) gave the desiredproduct as a white solid (9.79 mg, 0.0118 mmol, 48%). ¹H NMR (400 MHz,Methanol-d₄) δ 7.80 (dd, J=8.4, 7.4 Hz, 1H), 7.51 (dd, J=7.1, 1.5 Hz,1H), 7.48-7.34 (m, 5H), 5.11 (ddd, J=12.4, 5.4, 3.5 Hz, 1H), 4.76 (s,2H), 4.69 (td, J=7.2, 1.4 Hz, 1H), 3.76 (s, 3H), 3.55 (d, J=7.2 Hz, 2H),3.48-3.33 (m, 4H), 2.93-2.82 (m, 1H), 2.78-2.64 (m, 5H), 2.14-2.07 (m,1H), 1.96 (d, J=0.9 Hz, 3H), 1.66 (s, 4H). LCMS 829.39 (M+H).

Synthetic Example 23: Synthesis of dBET23

A 0.1 M solution ofN-(8-aminooctyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (220 microliters, 0.0220 mmol, 1 eq) was addedto(S)-4-(4-chlorophenyl)-6-(2-methoxy-2-oxoethyl)-3,9-dimethyl-6H-thieno[3,2-J][1,2,4]triazolo[4,3-a][1,4]diazepine-2-carboxylicacid (9.87 mg, 0.0220 mmol, 1 eq) at room temperature. DIPEA (11.5microliters, 0.0660 mmol, 3 eq) and HATU (8.4 mg, 0.0220 mmol, 1 eq)were added. The mixture was then stirred for 21 hours, then diluted withEtOAc and washed with saturated sodium bicarbonate, water and brine. Theorganic layer was dried over sodium sulfate, filtered and concentratedunder reduced pressure. Purification by column chromatography (ISCO, 4 gsilica column, 0-10% MeOH/DCM, 25 minute gradient) gave the desiredproduct as a white solid (8.84 mg, 0.00998 mmol, 45%). ¹H NMR (400 MHz,Methanol-d₄) δ 7.81 (dd, J=8.4, 7.4 Hz, 1H), 7.53 (d, J=7.3 Hz, 1H),7.50-7.39 (m, 5H), 5.12 (dd, J=12.6, 5.4 Hz, 1H), 4.75 (s, 2H), 4.68 (t,J=7.2 Hz, 1H), 3.76 (s, 3H), 3.54 (d, J=7.2 Hz, 2H), 3.39-3.32 (m, 3H),3.29 (s, 1H), 2.90-2.83 (m, 1H), 2.79-2.68 (m, 5H), 2.14 (dd, J=8.9, 3.7Hz, 1H), 1.99 (s, 3H), 1.65-1.53 (m, 4H), 1.36 (d, J=6.5 Hz, 8H). LCMS885.47 (M+H).

Synthetic Example 24: Synthesis of dBET24 Step 1: Synthesis oftert-butyl(2-(2-(2-(2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamido)ethoxy)ethoxy)ethyl)carbamate

2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetic acid(200 mg, 0.602 mmol, 1 eq) was dissolved in DMF (6.0 mL, 0.1M). HATU(228.9 mg, 0.602 mmol, 1 eq), DIPEA (0.315 mL, 1.81 mmol, 3 eq) andN-Boc-2,2′-(ethylenedioxy)diethylamine (0.143 mL, 0.602 mmol, 1 eq) wereadded sequentially. After 6 hours, additional HATU (114 mg, 0.30 mmol,0.5 eq) were added to ensure completeness of reaction. After anadditional 24 hours, the mixture was diluted with EtOAc, and washed withsaturated sodium bicarbonate, water and twice with brine. The combinedorganic layer was dried over sodium sulfate, filtered and concentratedunder reduced pressure. Purification by column chromatography (ISCO, 12g silica column, 0-15% MeOH/DCM, 15 minute gradient) gave the desiredproduct as a yellow oil (0.25 g, 0.44 mmol, 74%). ¹H NMR (400 MHz,Methanol-d₄) δ 7.82-7.75 (m, 1H), 7.51 (d, J=7.4 Hz, 1H), 7.41 (d, J=8.5Hz, 1H), 5.13 (dd, J=12.4, 5.5 Hz, 1H), 4.76 (s, 2H), 3.66-3.58 (m, 6H),3.53-3.45 (m, 4H), 3.19 (t, J=5.6 Hz, 2H), 2.95-2.83 (m, 1H), 2.80-2.67(m, 2H), 2.19-2.12 (m, 1H), 1.41 (s, 9H). LCMS 563.34 (M+H).

Step 2: Synthesis ofN-(2-(2-(2-aminoethoxy)ethoxy)ethyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate

tert-butyl(2-(2-(2-(2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamido)ethoxy)ethoxy)ethyl)carbamate(0.25 g, 0.44 mmol, 1 eq) was dissolved in TFA (4.5 mL) and heated to50° C. After 3 hours, the mixture was cooled to room temperature,diluted with MeOH, and concentrated under reduced pressure. Purificationby preparative HPLC gave the desired product as a tan solid (0.197 g,0.342 mmol, 77%). ¹H NMR (400 MHz, Methanol-d₄) δ 7.81 (ddd, J=8.4, 7.4,1.1 Hz, 1H), 7.55-7.50 (m, 1H), 7.43 (d, J=8.5 Hz, 1H), 5.13 (dd,J=12.7, 5.5 Hz, 1H), 4.78 (s, 2H), 3.74-3.66 (m, 6H), 3.64 (t, J=5.4 Hz,2H), 3.52 (t, J=5.3 Hz, 2H), 3.14-3.08 (m, 2H), 2.89 (ddd, J=17.5, 13.9,5.2 Hz, 1H), 2.80-2.66 (m, 2H), 2.16 (dtd, J=13.0, 5.7, 2.7 Hz, 1H).LCMS 463.36 (M+H).

Step 2: Synthesis of dBET24

A 0.1 M solution ofN-(2-(2-(2-aminoethoxy)ethoxy)ethyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (0.324 mL, 0.0324 mmol, 1 eq) was added toJQ-acid (13.0 mg, 0.324 mmol, 1 eq). DIPEA 16.9 microliters, 0.0972mmol, 3 eq) and HATU (12.3 mg, 0.0324 mmol, 1 eq) were then added andthe mixture was stirred for 18 hours at room temperature. The mixturewas then diluted with EtOAc and washed with saturated sodiumbicarbonate, water and brine. The organic layer was then dried oversodium sulfate, filtered and concentrated under reduced pressure.Purification by column chromatography (ISCO, 4 g silica column, 0-10%MeOH/DCM, 25 minute gradient) gave the desired product as an off-whitesolid (20.0 mg, 0.0236 mmol, 73%). ¹H NMR (400 MHz, Methanol-d₄) δ7.77-7.72 (m, 1H), 7.49 (d, J=7.4 Hz, 1H), 7.45-7.35 (m, 5H), 5.09 (ddd,J=12.3, 5.4, 3.7 Hz, 1H), 4.76 (s, 2H), 4.60 (dd, J=8.9, 5.3 Hz, 1H),3.68-3.62 (m, 6H), 3.59 (t, J=5.6 Hz, 2H), 3.54-3.48 (m, 2H), 3.47-3.35(m, 4H), 2.84 (ddd, J=19.4, 9.9, 4.6 Hz, 1H), 2.77-2.69 (m, 2H), 2.68(d, J=1.8 Hz, 3H), 2.43 (s, 3H), 2.12 (dt, J=9.8, 5.3 Hz, 1H), 1.68 (s,3H). LCMS 845.39 (M+H).

Synthetic Example 25: Synthesis of dBET25

A 0.1 M solution ofN-(4-aminobutyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (183 microliters, 0.0183 mmol, 1 eq) was addedto(S)-4-(4-chlorophenyl)-6-(2-methoxy-2-oxoethyl)-2,9-dimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepine-3-carboxylicacid (8.16 mg, 0.0183 mmol, 1 eq) at room temperature. DIPEA (9.6microliters, 0.0550 mmol, 3 eq) and HATU (7.0 mg, 0.0183 mmol, 1 eq)were added. The mixture was then stirred for 23 hours, then diluted withEtOAc and washed with saturated sodium bicarbonate, water and brine. Theorganic layer was dried over sodium sulfate, filtered and concentratedunder reduced pressure. Purification by column chromatography (ISCO, 4 gsilica column, 0-10% MeOH/DCM, 25 minute gradient) gave the desiredproduct as a yellow solid (4.39 mg, 0.00529 mmol, 29%). ¹H NMR (400 MHz,Methanol-d₄) δ 7.82 (dd, J=8.4, 7.4 Hz, 1H), 7.55 (d, J=7.3 Hz, 1H),7.45 (d, J=8.2 Hz, 1H), 7.43-7.31 (m, 4H), 5.16-5.10 (m, 1H), 4.77 (d,J=1.5 Hz, 2H), 4.56 (s, 1H), 3.74 (d, J=1.8 Hz, 3H), 3.66-3.60 (m, 1H),3.50 (dd, J=16.5, 7.3 Hz, 1H), 3.37-3.32 (m, 1H), 3.28 (s, 3H), 2.85 (t,J=7.2 Hz, 2H), 2.75 (d, J=7.8 Hz, 1H), 2.71 (d, J=0.9 Hz, 3H), 2.59 (d,J=1.0 Hz, 3H), 2.18-2.10 (m, 1H), 1.36-1.24 (m, 4H). LCMS 829.38 (M+H).

Synthetic Example 26: Synthesis of dBET26

A 0.1 M solution ofN-(8-aminooctyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (186 microliters, 0.0186 mmol, 1 eq) was addedto(S)-4-(4-chlorophenyl)-6-(2-methoxy-2-oxoethyl)-2,9-dimethyl-6H-thieno[3,2-j][1,2,4]triazolo[4,3-a][1,4]diazepine-3-carboxylicacid (8.26 mg, 0.0186 mmol, 1 eq) at room temperature. DIPEA (9.7microliters, 0.0557 mmol, 3 eq) and HATU (7.1 mg, 0.0186 mmol, 1 eq)were added. The mixture was then stirred for 23 hours, then diluted withEtOAc and washed with saturated sodium bicarbonate, water and brine. Theorganic layer was dried over sodium sulfate, filtered and concentratedunder reduced pressure. Purification by column chromatography (ISCO, 4 gsilica column, 0-10% MeOH/DCM, 25 minute gradient) gave the desiredproduct as a cream colored solid (6.34 mg, 0.00716 mmol, 38%). ¹H NMR(400 MHz, Methanol-d₄) δ 7.83-7.78 (m, 1H), 7.53 (dd, J=7.3, 2.2 Hz,1H), 7.45-7.38 (m, 3H), 7.32 (dd, J=8.5, 1.3 Hz, 2H), 5.16-5.08 (m, 1H),4.76 (s, 2H), 4.56 (s, 1H), 3.75 (s, 3H), 3.66 (dd, J=15.9, 8.7 Hz, 1H),3.50 (dd, J=16.9, 6.9 Hz, 1H), 3.32 (d, J=2.8 Hz, 4H), 2.84-2.74 (m,3H), 2.70 (d, J=1.1 Hz, 3H), 2.66-2.54 (m, 3H), 2.14 (d, J=5.3 Hz, 1H),1.62-1.22 (m, 12H). LCMS 885.48 (M+H).

Synthetic Example 27: Synthesis of dBET27

A 0.1 M solution of4-(2-(2-aminoethoxy)ethoxy)-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dionetrifluoroacetate in DMF (257 microliters, 0.0257 mmol, 1 eq) was addedto JQ-acid (10.3 mg, 0.0257 mmol, 1 eq). DIPEA (13.4 microliters, 0.0771mmol, 3 eq) and HATU (9.8 mg, 0.0257 mmol, 1 eq) were then added and themixture was stirred for 18 hours at room temperature. The mixture wasthen diluted with EtOAc and washed with saturated sodium bicarbonate,water and brine. The organic layer was then dried over sodium sulfate,filtered and concentrated under reduced pressure. Purification by columnchromatography (ISCO, 4 g silica column, 0-10% MeOH/DCM, 25 minutegradient) gave the desired product as a white solid (14.53 mg, 0.0195mmol, 76%). ¹H NMR (400 MHz, Methanol-d₄) δ 7.75 (ddd, J=8.5, 7.3, 1.3Hz, 1H), 7.47-7.30 (m, 6H), 5.00 (ddd, J=25.4, 12.2, 5.2 Hz, 1H), 4.61(td, J=9.4, 5.0 Hz, 1H), 4.36 (q, J=4.8 Hz, 2H), 3.96-3.89 (m, 2H), 3.74(q, J=5.6 Hz, 2H), 3.53-3.41 (m, 3H), 3.30-3.24 (m, 1H), 2.78-2.53 (m,6H), 2.41 (d, J=3.9 Hz, 3H), 2.09-1.98 (m, 1H), 1.67 (d, J=5.0 Hz, 3H).

Synthetic Example 28: Synthesis of dBET28

A 0.1 M solution of4-(4-aminobutoxy)-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dionetrifluoroacetate in DMF (202 microliters, 0.0202 mmol, 1 eq) was addedto JQ-acid (8.1 mg, 0.0202 mmol, 1 eq). DIPEA (10.6 microliters, 0.0606mmol, 3 eq) and HATU (7.7 mg, 0.0202 mmol, 1 eq) were then added and themixture was stirred for 18.5 hours at room temperature. The mixture wasthen diluted with EtOAc and washed with saturated sodium bicarbonate,water and brine. The organic layer was then dried over sodium sulfate,filtered and concentrated under reduced pressure. Purification by columnchromatography (ISCO, 4 g silica column, 0-10% MeOH/DCM, 25 minutegradient) gave the desired product as a cream colored solid (10.46 mg,0.0144 mmol, 71%). ¹H NMR (400 MHz, Methanol-d₄) δ 7.76 (t, J=7.5 Hz,1H), 7.43 (td, J=6.5, 2.5 Hz, 4H), 7.34 (t, J=8.8 Hz, 2H), 5.08-4.98 (m,1H), 4.64 (td, J=9.1, 5.0 Hz, 1H), 4.26 (t, J=5.3 Hz, 2H), 3.57-3.32 (m,4H), 2.84-2.59 (m, 6H), 2.45-2.37 (m, 3H), 2.08-2.01 (m, 1H), 2.00-1.91(m, 2H), 1.82 (dq, J=13.8, 6.9 Hz, 2H), 1.68 (d, J=11.7 Hz, 3H). LCMS728.38 (M+H).

Synthetic Example 29: Synthesis of dBET29

A 0.1 M solution of4-((6-aminohexyl)oxy)-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dionein DMF (205 microliters, 0.0205 mmol, 1 eq) was added to JQ-acid (8.2mg, 0.0205 mmol, 1 eq). DIPEA (10.7 microliters, 0.0614 mmol, 3 eq) andHATU (7.8 mg, 0.0205 mmol, 1 eq) were then added and the mixture wasstirred for 19 hours at room temperature. The mixture was then dilutedwith EtOAc and washed with saturated sodium bicarbonate, water andbrine. The organic layer was then dried over sodium sulfate, filteredand concentrated under reduced pressure. Purification by columnchromatography (ISCO, 4 g silica column, 0-10% MeOH/DCM, 25 minutegradient) gave the desired product as a white solid (8.04 mg, 0.0106mmol, 52%). 1H NMR (400 MHz, Methanol-d₄) δ 7.75-7.71 (m, 1H), 7.51-7.34(m, 6H), 5.07 (ddd, J=12.1, 5.4, 2.4 Hz, 1H), 4.62 (dd, J=9.0, 5.2 Hz,1H), 4.22 (t, J=6.4 Hz, 2H), 3.44-3.32 (m, 2H), 3.29-3.21 (m, 2H),2.88-2.65 (m, 6H), 2.43 (s, 3H), 2.13-2.06 (m, 1H), 1.86 (dt, J=13.9,6.7 Hz, 2H), 1.68 (s, 3H), 1.59 (dq, J=14.2, 7.0 Hz, 4H), 1.54-1.45 (m,2H). LCMS 756.40 (M+H).

Synthetic Example 30: Synthesis of dBET30

A 0.1 M solution ofN-(4-aminobutyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (163 microliters, 0.0163 mmol, 1 eq) was addedto(S)-4-(4-chlorophenyl)-3,9-dimethyl-6-(2-((3-(4-methylpiperazin-1-yl)propyl)amino)-2-oxoethyl)-6H-thieno[3,2][1,2,4]triazolo[4,3-a][1,4]diazepine-2-carboxylicacid (9.31 mg, 0.0163 mmol, 1 eq) at room temperature. DIPEA (8.5microliters, 0.0490 mmol, 3 eq) and HATU (6.2 mg, 0.0163 mmol, 1 eq)were added. The mixture was then stirred for 23.5 hours, then purifiedby preparative HPLC to give the desired product as a yellow oil (11.48mg, 0.0107 mmol, 66%). ¹H NMR (400 MHz, Methanol-d₄) δ 7.82-7.78 (m,1H), 7.54-7.35 (m, 6H), 5.09 (td, J=12.7, 5.4 Hz, 1H), 4.77-4.70 (m,3H), 3.56-3.31 (m, 12H), 3.23 (dd, J=8.0, 6.0 Hz, 3H), 3.05 (d, J=3.2Hz, 2H), 2.93-2.81 (m, 5H), 2.78-2.63 (m, 5H), 2.15-2.05 (m, 2H),1.96-1.86 (m, 4H), 1.68 (s, 4H). LCMS 954.55 (M+H).

Synthetic Example 31: Synthesis of dBET31

A 0.1 M solution ofN-(8-aminooctyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (153 microliters, 0.0153 mmol, 1 eq) was addedto(S)-4-(4-chlorophenyl)-3,9-dimethyl-6-(2-((3-(4-methylpiperazin-1-yl)propyl)amino)-2-oxoethyl)-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepine-2-carboxylicacid (8.7 mg, 0.0153 mmol, 1 eq) at room temperature. DIPEA (7.9microliters, 0.0458 mmol, 3 eq) and HATU (5.8 mg, 0.0153 mmol, 1 eq)were added. The mixture was then stirred for 25 hours, then purified bypreparative HPLC to give the desired product as a nice brown (not likepoop brown, kind of like brick) oil (9.52 mg, 0.00847 mmol, 55%). ¹H NMR(400 MHz, Methanol-d₄) δ 7.81 (dd, J=8.4, 7.4 Hz, 1H), 7.59-7.40 (m,6H), 5.12 (dd, J=12.5, 5.4 Hz, 1H), 4.75 (s, 2H), 4.71 (t, J=7.4 Hz,1H), 3.53-3.34 (m, 8H), 3.29-3.11 (m, 6H), 3.03-2.61 (m, 13H), 2.15 (s,1H), 2.01-1.84 (m, 5H), 1.59 (s, 4H), 1.37 (s, 8H). LCMS 1010.62 (M+H).

Synthetic Example 32: Synthesis of dBET32

A 0.1 M solution ofN-(4-aminobutyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (180 microliters, 0.0180 mmol, 1 eq) was addedto4-(4-(4-((4-((3-(N-(tert-butyl)sulfamoyl)phenyl)amino)-5-methylpyrimidin-2-yl)amino)phenyl)piperazin-1-yl)-4-oxobutanoicacid (10.7 mg, 0.0180 mmol, 1 eq) at room temperature. DIPEA (9.4microliters, 0.0539 mmol, 3 eq) and HATU (6.8 mg, 0.0180 mmol, 1 eq)were added and the mixture was stirred for 19 hours. The mixture wasthen diluted with methanol and purified by preparative HPLC to give thedesired product as a brown oil (4.40 mg, 0.00449 mmol, 25%). ¹H NMR (500MHz, Methanol-d₄) δ 8.08 (d, J=13.6 Hz, 1H), 7.84-7.76 (m, 3H), 7.63 (s,1H), 7.57-7.51 (m, 2H), 7.41 (d, J=8.4 Hz, 1H), 7.22 (td, J=6.7, 2.2 Hz,2H), 7.03-6.97 (m, 2H), 5.14 (dd, J=12.5, 5.5 Hz, 1H), 4.76 (d, J=16.8Hz, 2H), 3.72 (dt, J=10.0, 5.2 Hz, 4H), 3.34-3.33 (m, 1H), 3.23-3.12 (m,5H), 2.97 (dd, J=8.8, 4.0 Hz, 3H), 2.80-2.69 (m, 4H), 2.64 (dd, J=7.6,5.5 Hz, 1H), 2.50 (t, J=6.8 Hz, 1H), 2.22 (dd, J=2.4, 0.9 Hz, 3H),2.17-2.11 (m, 1H), 1.67-1.52 (m, 4H), 1.18 (d, J=0.8 Hz, 9H). LCMS980.64 (M+H).

Synthetic Example 33: Synthesis of dBET33

A 0.1 M solution ofN-(8-aminooctyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (188 microliters, 0.0188 mmol, 1 eq) was addedto4-(4-(4-((4-((3-(N-(tert-butyl)sulfamoyl)phenyl)amino)-5-methylpyrimidin-2-yl)amino)phenyl)piperazin-1-yl)-4-oxobutanoicacid (10.8 mg, 0.0188 mmol, 1 eq) at room temperature. DIPEA (9.8microliters, 0.0564 mmol, 3 eq) and HATU (7.1 mg, 0.0188 mmol, 1 eq)were added and the mixture was stirred for 23 hours. The mixture wasthen diluted with methanol and purified by preparative HPLC to give thedesired product as a brown residue (7.41 mg, 0.00715 mmol, 38%). ¹H NMR(500 MHz, Methanol-d₄) δ 8.06 (s, 1H), 7.80 (ddd, J=10.5, 7.6, 3.2 Hz,3H), 7.65 (d, J=4.5 Hz, 1H), 7.57-7.51 (m, 2H), 7.41 (dd, J=8.4, 2.9 Hz,1H), 7.25 (td, J=6.7, 2.9 Hz, 2H), 7.02 (t, J=8.0 Hz, 2H), 5.16-5.09 (m,1H), 4.75 (d, J=9.5 Hz, 2H), 3.76 (dq, J=16.0, 5.3 Hz, 4H), 3.29-3.12(m, 7H), 3.00-2.67 (m, 7H), 2.51 (t, J=6.8 Hz, 1H), 2.22 (d, J=3.1 Hz,3H), 2.13 (dtd, J=10.4, 5.7, 3.1 Hz, 1H), 1.59-1.52 (m, 2H), 1.51-1.43(m, 2H), 1.32 (t, J=16.6 Hz, 8H), 1.18 (d, J=1.3 Hz, 9H). LCMS 1036.69(M+H).

Synthetic Example 34: Synthesis of dBET34

A 0.1 M solution ofN-(3-(2-(2-(3-aminopropoxy)ethoxy)ethoxy)propyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (173 microliters, 0.0173 mmol, 1 eq) was addedto4-(4-(4-((4-((3-(N-(tert-butyl)sulfamoyl)phenyl)amino)-5-methylpyrimidin-2-yl)amino)phenyl)piperazin-1-yl)-4-oxobutanoicacid (10.3 μg, 0.0173 mmol, 1 eq) at room temperature. DIPEA (9.0microliters, 0.0519 μmol, 3 eq) and HATU (6.6 mg, 0.0173 mmol, 1 eq)were added and the mixture was stirred for 25 hours. The mixture wasthen diluted with methanol and purified by preparative HPLC to give thedesired product as a brown residue (7.99 mg, 0.00718 μmol, 42o). ¹H NMR(500 MHz, Methanol-d₄) δ 8.06 (s, 1H), 7.83-7.76 (i, 3H), 7.65 (s, 1H),7.58-7.50 (m, 2H), 7.43 (dd, J=17.7, 8.4 Hz, 1H), 7.27-7.21 (i, 2H),7.02 (t, J=8.0 Hz, 2H), 5.13 (dt, J=12.7, 5.2 Hz, 1H), 4.76 (d, J=12.4Hz, 2H), 3.73 (q, J=6.3 Hz, 4H), 3.63 3.49 (i, 10H), 3.41 (q, J=6.6 Hz,2H), 3.27-3.15 (i, 5H), 3.01-2.81 (i, 4H), 2.79-2.63 (i, 5H), 2.50 (t,J=6.8 Hz, 1H), 2.22 (d, J=2.3 Hz, 3H), 2.17-2.11 (m, 1H), 1.88-1.70 (m,4H), 1.18 (d, J=1.2 Hz, 9H). LCMS 1112.74 (M+H).

Synthetic Example 35: Synthesis of dBET35

A 0.1 M solution ofN-(4-aminobutyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-4-yl)amino)acetamidetrifluoroacetate in DMF (185 microliters, 0.0185 mmol, 1 eq) was addedto JQ-acid (7.4 mg, 0.0185 mmol, 1 eq). DIPEA (9.6 microliters, 0.0554mmol, 3 eq) and HATU (7.0 mg, 0.0185 mmol, 1 eq) were then added and themixture was stirred for 17 hours at room temperature. The mixture wasthen diluted with EtOAc and washed with saturated sodium bicarbonate,water and brine. The organic layer was then dried over sodium sulfate,filtered and concentrated under reduced pressure. Purification by columnchromatography (ISCO, 4 g silica column, 0-15% MeOH/DCM, 25 minutegradient) gave the desired product as a white solid (2.71 mg, 0.00351mmol, 19%). ¹H NMR (500 MHz, Methanol-d₄) δ 7.48-7.37 (m, 4H), 7.34 (t,J=7.8 Hz, 1H), 7.14 (dd, J=7.4, 2.4 Hz, 1H), 6.67 (d, J=8.1 Hz, 1H),5.14 (td, J=13.5, 5.2 Hz, 1H), 4.66-4.60 (m, 1H), 4.59 (d, J=8.3 Hz,2H), 4.43-4.31 (m, 2H), 3.88 (s, 2H), 3.25 (dd, J=14.8, 7.1 Hz, 4H),2.94-2.72 (m, 3H), 2.68 (d, J=4.9 Hz, 3H), 2.49-2.40 (m, 4H), 2.21-2.12(m, 1H), 1.68 (s, 3H), 1.53 (s, 4H). LCMS 770.51 (M+H).

Synthetic Example 36: Synthesis of dBET36

A 0.1 M solution ofN-(4-aminobutyl)-2-(2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)acetamidetrifluoroacetate in DMF (222 microliters, 0.0222 mmol, 1 eq) was addedto JQ-acid (8.9 mg, 0.0222 mmol, 1 eq). DIPEA (11.6 microliters, 0.0666mmol, 3 eq) and HATU (8.4 mg, 0.0222 mmol, 1 eq) were then added and themixture was stirred for 17.5 hours at room temperature. The mixture wasthen diluted with EtOAc and washed with saturated sodium bicarbonate,water and brine. The organic layer was then dried over sodium sulfate,filtered and concentrated under reduced pressure. Purification by columnchromatography (ISCO, 4 g silica column, 0-15% MeOH/DCM, 25 minutegradient) gave the desired product as a white solid (12.42 mg, 0.0156mmol, 70%). ¹H NMR (500 MHz, Methanol-d₄) δ 7.80-7.74 (m, 2H), 7.68 (d,J=6.8 Hz, 1H), 7.42 (q, J=8.7 Hz, 4H), 5.11 (dt, J=12.3, 4.6 Hz, 1H),4.63 (dd, J=8.8, 5.5 Hz, 1H), 4.10-4.00 (m, 2H), 3.39 (ddd, J=14.9, 8.8,2.5 Hz, 1H), 3.30-3.21 (m, 5H), 2.88-2.76 (m, 1H), 2.74-2.65 (m, 5H),2.44 (s, 3H), 2.15-2.08 (m, 1H), 1.69 (s, 3H), 1.63-1.55 (m, 4H). LCMS769.49 (M+H).

Synthetic Example 37: Synthesis of dBET37

A 0.1 M solution of6-amino-N-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)methyl)hexanamidetrifluoroacetate in DMF (195 microliters, 0.0195 mmol, 1 eq) was addedto JQ-acid (7.8 mg, 0.0195 mmol, 1 eq). DIPEA (10.2 microliters, 0.0584mmol, 3 eq) and HATU (7.4 mg, 0.0195 mmol, 1 eq) were then added and themixture was stirred for 18 hours at room temperature. The mixture wasthen diluted with EtOAc and washed with saturated sodium bicarbonate,water and brine. The organic layer was then dried over sodium sulfate,filtered and concentrated under reduced pressure. Purification by columnchromatography (ISCO, 4 g silica column, 0-15% MeOH/DCM, 25 minutegradient) gave the desired product as a white solid (11.83 mg, 0.0151mmol, 77%). ¹H NMR (500 MHz, Methanol-d₄) δ 7.78-7.74 (m, 2H), 7.71 (dd,J=5.3, 3.5 Hz, 1H), 7.42 (q, J=8.5 Hz, 4H), 5.13 (dd, J=12.6, 5.5 Hz,1H), 4.82 (s, 2H), 4.63 (dd, J=8.8, 5.5 Hz, 1H), 3.40 (ddd, J=15.0, 8.8,1.6 Hz, 1H), 3.30-3.21 (m, 3H), 2.86 (ddd, J=18.4, 14.6, 4.8 Hz, 1H),2.74 (ddd, J=13.8, 10.1, 2.8 Hz, 2H), 2.69 (s, 3H), 2.44 (s, 3H), 2.30(t, J=7.4 Hz, 2H), 2.13 (dtd, J=12.9, 4.9, 2.3 Hz, 1H), 1.74-1.64 (m,5H), 1.59 (p, J=7.0 Hz, 2H), 1.46-1.38 (m, 2H). LCMS 783.47 (M+H).

Synthetic Example 38: Synthesis of dBET38 Step 1: Synthesis oftert-butyl(3-(3-(2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamido)propoxy)propyl)carbamate

tert-butyl (3-(3-aminopropoxy)propyl)carbamate (134.5 mg, 0.579 mmol, 1eq) was dissolved in DMF (5.79 ml, 0.05 M) then added to2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetic acid(192.38 mg, 0.579 mmol, 1 eq). DIPEA (0.28 ml, 1.74 mmol, 3 eq) and HATU(153.61 mg, 0.579 mmol, 1 eq) were added and the mixture was stirred for18 hours at room temperature. The mixture was then diluted with EtOAcand washed with saturated sodium bicarbonate, water then brine. Theorganic layer was dried over sodium sulfate, filtered and condensed togive a yellow oil (157.1 mg). The crude material was purified by columnchromatography (ISCO, 12 g silica column, 0 to 15% MeOH/DCM 25 minutegradient) to give a yellow oil (121.3 mg, 0.222 mmol, 38.27%). ¹H NMR(400 MHz, Methanol-d₄) δ 7.78 (dd, J=8.4, 7.4 Hz, 1H), 7.50 (d, J=7.3Hz, 1H), 7.41 (d, J=8.5 Hz, 1H), 5.13 (dd, J=12.4, 5.5 Hz, 1H), 4.75 (s,2H), 3.53-3.37 (m, 6H), 3.14-3.07 (m, 2H), 2.94-2.88 (m, 1H), 2.79-2.68(m, 2H), 2.16 (ddd, J=12.8, 6.6, 2.7 Hz, 1H), 1.81 (p, J=6.4 Hz, 2H),1.73-1.65 (m, 2H), 1.40 (s, 9H). LCMS 547.6 (M+H).

Step 2: Synthesis ofN-(3-(3-aminopropoxy)propyl)-2-((2-(2,6-dioxopuperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate salt

TFA (2.22 ml, 0.1 M) was added to tert-butyl(3-(3-(2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamido)propoxy)propyl)carbamate(121.3 mg, 0.222 mmol, 1 eq) and the mixture was stirred at 50° C. for 2hours. The mixture was then dissolved in MeOH and concentrated underreduced pressure to give a brown oil (114.1 mg) that was carried forwardwithout further purification. ¹H NMR (400 MHz, Methanol-d₄) δ 7.81-7.74(m, 1H), 7.50 (d, J=7.3 Hz, 1H), 7.41 (d, J=8.5 Hz, 1H), 5.12 (dd,J=12.7, 5.5 Hz, 1H), 4.76 (s, 2H), 3.57-3.52 (m, 2H), 3.48 (t, J=5.9 Hz,2H), 3.40 (t, J=6.6 Hz, 2H), 3.06 (t, J=6.5 Hz, 2H), 2.87 (ddd, J=14.1,10.1, 7.0 Hz, 1H), 2.79-2.65 (m, 2H), 2.15 (dtd, J=12.8, 5.5, 2.6 Hz,1H), 1.92 (dt, J=11.7, 5.9 Hz, 2H), 1.81 (p, J=6.3 Hz, 2H). LCMS 447.2(M+H).

Step 3: Synthesis of dBET38

A 0.1 M solution ofN-(3-(3-aminopropoxy)propyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (0.215 mL, 0.0215 mmol, 1 eq) was added toJQ-acid (8.6 mg, 0.0215 mmol, 1 eq) at room temperature. DIPEA (11.2microliters, 0.0644 mmol, 3 eq) and HATU (8.2 mg, 0.0215 mmol, 1 eq)were added. After 19 hours, the mixture was diluted with EtOAc andwashed with saturated sodium bicarbonate, water and brine. The combinedorganic layer was dried over sodium sulfate, filtered and concentratedunder reduced pressure. Purification by column chromatography (ISCO, 4 gsilica column, 0-15% MeOH/DCM, 25 minute gradient) gave the desiredproduct as a cream colored solid (10.6 mg, 0.0127 mmol, 59%). ¹H NMR(500 MHz, Methanol-d₄) δ 7.79-7.74 (m, 1H), 7.50 (d, J=8.1 Hz, 1H),7.46-7.36 (m, 5H), 5.11 (ddd, J=12.4, 5.5, 1.7 Hz, 1H), 4.73 (s, 2H),4.62 (ddd, J=8.7, 5.4, 1.4 Hz, 1H), 3.50 (q, J=6.3 Hz, 4H), 3.43 (t,J=6.5 Hz, 2H), 3.41-3.32 (m, 3H), 3.29-3.24 (m, 1H), 2.85 (ddd, J=18.3,14.6, 4.2 Hz, 1H), 2.77-2.65 (m, 5H), 2.43 (s, 3H), 2.17-2.09 (m, 1H),1.80 (h, J=6.4 Hz, 4H), 1.68 (s, 3H). LCMS 829.32 (M+H).

Synthetic Example 39: Synthesis of dBET39

A 0.1 M solution of4-(2-(2-(21-aminodecyl)oxy)-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dionetrifluoroacetate in DMF (0.212 mL, 0.0212 mmol, 1 eq) was added toJQ-acid (8.5 ig, 0.0212 mmol, 1 eq) at room temperature. DIPEA (11.1microliters, 0.0636 mmol, 3 eq) and HATU (8.1 mg, 0.0212 mmol, 1 eq)were added. After 19 hours, the mixture was diluted with EtOAc andwashed with saturated sodium bicarbonate, water and brine. The combinedorganic layer was dried over sodium sulfate, filtered and concentratedunder reduced pressure. Purification by column chromatography (ISCO, 4 gsilica column, 0-15% MeOH/DCM, 25 minute gradient) and preparative PLCgave the desired product (0.39 mg, 0.00048 mmol, 2.30). ¹H NMR (500 MHz,Methanol-d₄) δ 7.77-7.73 (m, 1H), 7.56-7.31 (i, 6H), 5.11-5.06 (i, 1H),4.62 (dd, J=9.2, 5.0 Hz, 1H), 4.58 (s, 2H), 4.21 (t, J=6.3 Hz, 2H),3.42-3.38 (m, 1H), 3.24-3.20 (m, 1H), 2.90-2.68 (i, 6H), 2.45 (d, J=6.7Hz, 3H), 2.11 (s, 1H), 1.83 (dd, J=14.7, 6.6 Hz, 2H), 1.70 (s, 3H),1.61-1.49 (m, 4H), 1.32 (d, J=23.2 Hz, 10H). LCMS 812.60 (M+H).

Synthetic Example 40: Synthesis of dBET4O

A 0.1 M solution of4-(2-(2-(2-aminoethoxy)ethoxy)ethoxy)-2-(2,6-dioxopiperidin-3-yl)isoindoline-1,3-dionetrifluoroacetate in DMF (0.242 mL, 0.0242 mmol, 1 eq) was added toJQ-acid (9.7 mg, 0.0242 mmol, 1 eq) at room temperature. DIPEA (12.6microliters, 0.0726 mmol, 3 eq) and HATU (9.2 mg, 0.0242 mmol, 1 eq)were added. After 22 hours, the mixture was diluted with EtOAc andwashed with saturated sodium bicarbonate, water and brine. The combinedorganic layer was dried over sodium sulfate, filtered and concentratedunder reduced pressure. Purification by column chromatography (ISCO, 4 gsilica column, 0-10% MeOH/DCM, 25 minute gradient) and preparative HPLCgave the desired product as a brown oil (4.74 mg, 0.00601 mmol, 25%). ¹HNMR (500 MHz, Methanol-d₄) δ 7.77-7.67 (m, 1H), 7.52-7.36 (m, 5H),5.09-5.03 (m, 1H), 4.64 (d, J=4.8 Hz, 1H), 4.40-4.32 (m, 2H), 3.97-3.88(m, 2H), 3.81-3.74 (m, 2H), 3.69-3.60 (m, 5H), 3.55-3.38 (m, 4H),2.89-2.54 (m, 6H), 2.45 (d, J=5.9 Hz, 3H), 2.11 (s, 1H), 1.70 (d, J=8.6Hz, 3H). LCMS 788.42 (M+H).

Synthetic Example 41: Synthesis of dBET41 Step 1: Synthesis oftert-butyl(4-((2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamido)methyl)benzyl)carbamate

tert-butyl (4-(aminomethyl)benzyl)carbamate (183.14 mg, 0.755 mmol, 1eq) was dissolved in DMF (15.1 ml, 0.05 M) and added to2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetic acid(250.90 mg, 0.755 mmol, 1 eq). DIPEA (0.374 ml, 2.265 mmol, 3 eq) andHATU (296.67 mg, 0.755 mmol, 1 eq) were added and the mixture wasstirred for 20 hours at room temperature. The mixture was then dilutedwith EtOAc and washed with saturated sodium bicarbonate, water thenbrine. The organic layer was dried over sodium sulfate, filtered andcondensed to give a light brown oil. The crude material was purified bycolumn chromatography (ISCO, 12 g silica column, 0 to 15% MeOH/DCM 25minute gradient) to give a light brown oil (373.1 mg, 0.678 mmol,89.8%). ¹H NMR (500 MHz, DMSO-d₆) δ 11.10 (s, 2H), 8.48 (t, J=5.8 Hz,1H), 7.80 (dd, J=8.4, 7.3 Hz, 1H), 7.49 (d, J=7.2 Hz, 1H), 7.40 (d,J=8.6 Hz, 1H), 7.26-7.08 (m, 4H), 5.11 (dd, J=12.9, 5.4 Hz, 1H), 4.86(s, 2H), 4.33 (d, J=3.9 Hz, 2H), 4.09 (d, J=5.3 Hz, 2H), 2.65-2.51 (m,3H), 2.07-1.99 (m, 1H), 1.38 (s, 9H). LCMS 551.5 (M+H).

Step 2: Synthesis ofN-(4-(aminomethyl)benzyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoracetate salt

TFA (6.77 ml, 0.1 M) was added to tert-butyl(4-((2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamido)methyl)benzyl)carbamate(373.1 mg, 0.677 mmol, 1 eq) and the mixture was stirred at 50° C. for1.5 hours. The mixture was then dissolved in MeOH and concentrated underreduced pressure to give a brown oil (270.29 mg) that was carriedforward without further purification. ¹H NMR (500 MHz, DMSO-d₆) δ 11.11(s, 1H), 8.55 (t, J=6.2 Hz, 1H), 8.07 (s, 3H), 7.81 (dd, J=8.5, 7.3 Hz,1H), 7.51 (d, J=7.2 Hz, 1H), 7.40 (dd, J=14.9, 8.3 Hz, 3H), 7.31 (d,J=8.2 Hz, 2H), 5.11 (dd, J=12.9, 5.4 Hz, 1H), 4.87 (s, 2H), 4.37 (d,J=6.1 Hz, 2H), 4.01 (q, J=5.8 Hz, 2H), 2.66-2.51 (m, 3H), 2.07-1.99 (m,1H). LCMS 451.3 (M+H).

Step 3: Synthesis of dBET41

A 0.1 M solution ofN-(4-(aminomethyl)benzyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (0.237 mL, 0.0237 mmol, 1 eq) was added toJQ-acid (9.5 mg, 0.0237 mmol, 1 eq) at room temperature. After 23 hours,the mixture was diluted with EtOAc and washed with saturated sodiumbicarbonate, water and brine. The organic layer was dried over sodiumsulfate, filtered and concentrated under reduced pressure. Purificationby column chromatography (ISCO, 4 g silica column, 0-10% MeOH/DCM, 25minute gradient) gave the desired product as a cream colored solid (11.8mg, 0.0142 mmol, 60%). 1H NMR (500 MHz, Methanol-d₄) δ 7.80-7.75 (m,1H), 7.51 (dd, J=7.3, 1.5 Hz, 1H), 7.41 (d, J=8.4 Hz, 1H), 7.36 (d,J=2.2 Hz, 4H), 7.34-7.28 (m, 4H), 5.10-5.00 (m, 1H), 4.82 (s, 2H),4.67-4.64 (m, 1H), 4.61-4.42 (m, 4H), 4.34 (dd, J=14.9, 12.8 Hz, 1H),3.49 (ddd, J=14.8, 9.5, 5.2 Hz, 1H), 2.83-2.75 (m, 1H), 2.73-2.61 (i,5H), 2.44-2.39 (m, 3H), 2.06 (ddq, J=9.8, 4.7, 2.6 Hz, 1H), 1.66 (d,J=4.2 Hz, 3H). LCMS 832.92 (M+H).

Synthetic Example 42: Synthesis of dBET42

A 0.1 M solution of5-amino-N-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-4-yl)pentanamidetrifluoroacetate in DMF (222 microliters, 0.0222 mmol, 1 eq) was addedto JQ-acid (8.9 mg, 0.0222 mmol, 1 eq). DIPEA (11.6 microliters, 0.0666mmol, 3 eq) and HATU (8.4 mg, 0.0222 mmol, 1 eq) were then added and themixture was stirred for 24 hours at room temperature. The mixture wasthen diluted with EtOAc and washed with saturated sodium bicarbonate,water and brine. The organic layer was then dried over sodium sulfate,filtered and concentrated under reduced pressure. Purification by columnchromatography (ISCO, 4 g silica column, 0-10% MeOH/DCM, 25 minutegradient) gave the desired product as a white solid (12.23 mg, 0.0165mmol, 74%). ¹H NMR (500 MHz, Methanol-d₄) δ 7.76-7.71 (m, 1H), 7.66-7.62(m, 1H), 7.51 (td, J=7.8, 2.5 Hz, 1H), 7.45-7.35 (m, 4H), 5.11 (ddd,J=13.2, 11.3, 5.2 Hz, 1H), 4.63 (ddd, J=8.8, 5.7, 3.2 Hz, 1H), 4.47 (s,2H), 3.45-3.32 (m, 3H), 3.30-3.27 (m, 1H), 2.90-2.80 (m, 1H), 2.73-2.63(m, 4H), 2.49 (t, J=7.4 Hz, 2H), 2.46-2.38 (m, 4H), 2.11 (ddtd, J=12.8,10.5, 5.3, 2.3 Hz, 1H), 1.84-1.75 (m, 2H), 1.66 (dd, J=16.2, 7.6 Hz,5H). LCMS 741.46 (M+H).

Synthetic Example 43: Synthesis of dBET43

A 0.1 M solution of7-amino-N-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-4-yl)heptanamidetrifluoroacetate in DMF (227 microliters, 0.0227 mmol, 1 eq) was addedto JQ-acid (9.1 mg, 0.0227 mmol, 1 eq). DIPEA (11.9 microliters, 0.0681mmol, 3 eq) and HATU (8.6 mg, 0.0227 mmol, 1 eq) were then added and themixture was stirred for 25.5 hours at room temperature. The mixture wasthen diluted with EtOAc and washed with saturated sodium bicarbonate,water and brine. The organic layer was then dried over sodium sulfate,filtered and concentrated under reduced pressure. Purification by columnchromatography (ISCO, 4 g silica column, 0-10% MeOH/DCM, 25 minutegradient) gave the desired product as an off-white solid (12.58 mg,0.0164 mmol, 72%). ¹H NMR (500 MHz, Methanol-d₄) δ 7.71 (d, J=7.9 Hz,1H), 7.64 (d, J=7.4 Hz, 1H), 7.51 (t, J=7.8 Hz, 1H), 7.46-7.38 (m, 4H),5.14 (ddd, J=13.3, 5.2, 2.2 Hz, 1H), 4.62 (ddd, J=8.6, 5.6, 2.1 Hz, 1H),4.49-4.45 (m, 2H), 3.39 (ddd, J=14.9, 8.7, 1.3 Hz, 1H), 3.30-3.24 (m,3H), 2.93-2.83 (m, 1H), 2.79-2.65 (m, 4H), 2.50-2.40 (m, 6H), 2.16 (ddq,J=9.9, 5.2, 2.6 Hz, 1H), 1.78-1.70 (m, 2H), 1.68 (d, J=2.1 Hz, 3H),1.63-1.57 (m, 2H), 1.50-1.42 (m, 4H). LCMS 769.55 (M+H).

Synthetic Example 44: Synthesis of dBET44

A 0.1 M solution of8-amino-N-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-4-yl)octanamidetrifluoroacetate in DMF (217 microliters, 0.0217 mmol, 1 eq) was addedto JQ-acid (8.7 mg, 0.0217 mmol, 1 eq). DIPEA (11.3 microliters, 0.0651mmol, 3 eq) and HATU (8.3 mg, 0.0217 mmol, 1 eq) were then added and themixture was stirred for 20.5 hours at room temperature. The mixture wasthen diluted with EtOAc and washed with saturated sodium bicarbonate,water and brine. The organic layer was then dried over sodium sulfate,filtered and concentrated under reduced pressure. Purification by columnchromatography (ISCO, 4 g silica column, 0-10% MeOH/DCM, 25 minutegradient) gave the desired product as an cream colored solid (14.28 mg,0.0182 mmol, 84%). ¹H NMR (500 MHz, Methanol-d₄) δ 7.72-7.68 (m, 1H),7.64 (d, J=7.5 Hz, 1H), 7.51 (t, J=7.7 Hz, 1H), 7.46-7.39 (m, 4H), 5.14(dt, J=13.3, 5.0 Hz, 1H), 4.62 (dd, J=8.8, 5.4 Hz, 1H), 4.48-4.44 (m,2H), 3.40 (ddd, J=14.9, 8.8, 0.9 Hz, 1H), 3.26 (dt, J=13.2, 6.9 Hz, 3H),2.88 (ddd, J=18.7, 13.5, 5.4 Hz, 1H), 2.75 (dddd, J=17.6, 7.1, 4.5, 2.4Hz, 1H), 2.68 (d, J=2.2 Hz, 3H), 2.49-2.39 (m, 6H), 2.17 (ddt, J=9.8,5.3, 2.3 Hz, 1H), 1.76-1.70 (m, 2H), 1.70-1.67 (m, 3H), 1.61-1.54 (m,2H), 1.42 (s, 6H). LCMS 783.53 (M+H).

Synthetic Example 45: Synthesis of dBET45

A 0.1 M solution ofN-(8-aminooctyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (268 microliters, 0.0268 mmol, 1 eq) was addedto(R)-4-((4-cyclopentyl-1,3-dimethyl-2-oxo-1,2,3,4-tetrahydropyrido[2,3-b]pyrazin-6-yl)amino)-3-methoxybenzoicacid (11.0 mg, 0.0268 mmol, 1 eq) at room temperature. DIPEA (14.0microliters, 0.0804 mmol, 3 eq) and HATU (10.2 mg, 0.0268 mmol, 1 eq)were then added and the mixture was stirred for 18.5 hours. The mixturewas then diluted with methanol and purified by preparative HPLC to givethe desired product as a dark brown solid (10.44 mg, 0.0108 mmol, 40%).¹H NMR (500 MHz, Methanol-d₄) δ 8.38 (d, J=8.4 Hz, 1H), 7.80-7.75 (m,1H), 7.55-7.48 (m, 1H), 7.48-7.35 (m, 3H), 7.27 (d, J=8.3 Hz, 1H), 6.45(d, J=8.2 Hz, 1H), 5.12 (dd, J=12.5, 5.5 Hz, 1H), 4.72 (d, J=5.1 Hz,2H), 4.53 (s, 1H), 4.28 (d, J=6.8 Hz, 1H), 3.98 (d, J=4.1 Hz, 3H),3.48-3.33 (m, 4H), 2.90-2.82 (m, 1H), 2.80-2.69 (m, 2H), 2.18-2.01 (m,4H), 1.88-1.52 (m, 10H), 1.34 (d, J=42.9 Hz, 10H), 1.17 (d, J=6.8 Hz,3H). LCMS 851.67 (M+H).

Synthetic Example 46: Synthesis of dBET46

A 0.1 M solution ofN-(3-(2-(2-(3-aminopropoxy)ethoxy)ethoxy)propyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (256 microliters, 0.0256 mmol, 1 eq) was addedto(R)-4-((4-cyclopentyl-1,3-dimethyl-2-oxo-1,2,3,4-tetrahydropyrido[2,3-b]pyrazin-6-yl)amino)-3-methoxybenzoicacid (10.5 mg, 0.0256 mmol, 1 eq) at room temperature. DIPEA (13.4microliters, 0.0767 mmol, 3 eq) and HATU (9.7 mg, 0.0256 mmol, 1 eq)were then added and the mixture was stirred for 20 hours. The mixturewas then diluted with methanol and purified by preparative HPLC to givethe desired product as a dark brown solid (13.69 mg, 0.0132 mmol, 51%).¹H NMR (500 MHz, Methanol-d₄) δ 8.28-8.24 (m, 1H), 7.74-7.71 (m, 1H),7.49 (dd, J=7.3, 3.7 Hz, 1H), 7.39-7.34 (m, 2H), 7.28-7.25 (m, 1H),7.14-7.10 (m, 1H), 6.34 (d, J=8.3 Hz, 1H), 5.01-4.97 (m, 1H), 4.62 (s,2H), 4.25 (q, J=6.7 Hz, 1H), 3.95 (d, J=5.4 Hz, 3H), 3.60 (ddd, J=9.0,6.1, 1.6 Hz, 8H), 3.53-3.46 (m, 6H), 3.40-3.37 (m, 2H), 2.78 (td,J=11.1, 6.6 Hz, 3H), 2.16-2.00 (m, 4H), 1.84 (ddt, J=33.5, 13.0, 6.4 Hz,7H), 1.75-1.60 (m, 6H), 1.17 (d, J=6.8 Hz, 3H). LCMS 927.74 (M+H).

Synthetic Example 47: Synthesis of dBET50

A 0.1 M solution ofN-(3-(2-(2-(3-aminopropoxy)ethoxy)ethoxy)propyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (200 microliters, 0.0200 mmol, 1 eq) was addedto(S)-4-(4-chlorophenyl)-6-(2-methoxy-2-oxoethyl)-3,9-dimethyl-6H-thieno[3,2-][1,2,4]triazolo[4,3-a][1,4]diazepine-2-carboxylicacid (8.9 mg, 0.020 mmol, 1 eq) at room temperature. DIPEA (10.5microliters, 0.060 mmol, 3 eq) and HATU (7.6 mg, 0.020 mmol, 1 eq) wereadded. The mixture was then stirred for 17 hours, then diluted withEtOAc and washed with saturated sodium bicarbonate, water and brine. Theorganic layer was dried over sodium sulfate, filtered and concentratedunder reduced pressure. Purification by column chromatography (ISCO, 4 gsilica column, 0-10% MeOH/DCM, 25 minute gradient) gave the desiredproduct as a cream colored solid (9.31 mg, 0.00968 mmol, 48%). ¹H NMR(500 MHz, Methanol-d₄) δ 7.82-7.78 (m, 1H), 7.52 (dd, J=7.1, 1.6 Hz,1H), 7.49-7.40 (m, 5H), 5.10 (ddd, J=12.8, 5.5, 2.9 Hz, 1H), 4.74 (s,2H), 4.67 (t, J=7.1 Hz, 1H), 3.76 (s, 3H), 3.62-3.50 (m, 14H), 3.49-3.43(m, 2H), 3.40 (q, J=6.5 Hz, 2H), 2.87 (ddd, J=17.6, 14.0, 5.3 Hz, 1H),2.79-2.67 (m, 5H), 2.12 (ddq, J=10.3, 5.4, 2.9 Hz, 1H), 2.00 (s, 3H),1.86 (q, J=6.3 Hz, 2H), 1.80 (p, J=6.4 Hz, 2H). LCMS 961.67 (M+H).

Synthetic Example 48: Synthesis of dBET51

A 0.1 M solution ofN-(2-(2-(2-aminoethoxy)ethoxy)ethyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (200 microliters, 0.0200 mmol, 1 eq) was addedto(S)-4-(4-chlorophenyl)-6-(2-methoxy-2-oxoethyl)-3,9-dimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepine-2-carboxylicacid (8.9 mg, 0.020 mmol, 1 eq) at room temperature. DIPEA (10.5microliters, 0.060 mmol, 3 eq) and HATU (7.6 mg, 0.020 mmol, 1 eq) wereadded. The mixture was then stirred for 17 hours, then diluted withEtOAc and washed with saturated sodium bicarbonate, water and brine. Theorganic layer was dried over sodium sulfate, filtered and concentratedunder reduced pressure. Purification by column chromatography (ISCO, 4 gsilica column, 0-10% MeOH/DCM, 25 minute gradient) gave the desiredproduct as an off-white solid (8.38 mg, 0.00942 mmol, 47%). ¹H NMR (500MHz, Methanol-d₄) δ 7.80 (dd, J=8.4, 7.4 Hz, 1H), 7.52 (dd, J=7.2, 1.3Hz, 1H), 7.48-7.38 (m, 5H), 5.08 (ddd, J=12.7, 5.5, 1.6 Hz, 1H), 4.74(d, J=2.7 Hz, 2H), 4.66 (t, J=7.1 Hz, 1H), 3.75 (d, J=3.0 Hz, 3H), 3.65(t, J=4.1 Hz, 6H), 3.59 (t, J=5.3 Hz, 2H), 3.57-3.49 (m, 4H), 3.49-3.40(m, 2H), 2.93-2.84 (m, 1H), 2.78-2.64 (m, 5H), 2.15-2.09 (m, 1H), 2.00(d, J=0.9 Hz, 3H). LCMS 889.58 (M+H).

Synthetic Example 49: Synthesis of dBET52

A 0.1 M solution ofN-(2-(2-(2-(2-aminoethoxy)ethoxy)ethoxy)ethyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (200 microliters, 0.020 mmol, 1 eq) was added toJQ-acid (8.0 mg, 0.020 mmol, 1 eq) at room temperature. DIPEA (10.5microliters, 0.060 mmol, 3 eq) and HATU (7.6 mg, 0.020 mmol, 1 eq) wereadded. After 17.5 hours, the mixture was diluted with EtOAc and washedwith saturated sodium bicarbonate, water and brine. The combined organiclayer was dried over sodium sulfate, filtered and concentrated underreduced pressure. Purification by column chromatography (ISCO, 4 gsilica column, 0-10% MeOH/DCM, 25 minute gradient) gave the desiredproduct as a colorless residue (9.12 mg, 0.01025 mmol, 51%). ¹H NMR (500MHz, Methanol-d₄) δ 7.77 (t, J=7.9 Hz, 1H), 7.50 (dd, J=7.3, 1.5 Hz,1H), 7.47-7.36 (m, 5H), 5.09 (ddd, J=13.0, 7.6, 5.5 Hz, 1H), 4.76 (s,2H), 4.62 (dd, J=9.1, 5.1 Hz, 1H), 3.62 (ddt, J=17.3, 11.2, 6.5 Hz,12H), 3.52-3.41 (m, 5H), 3.28 (d, J=5.1 Hz, 1H), 2.90-2.81 (m, 1H),2.79-2.66 (m, 5H), 2.44 (s, 3H), 2.16-2.09 (m, 1H), 1.69 (s, 3H). LCMS889.38 (M+H).

Synthetic Example 50: Synthesis of dBET53

A 0.1 M solution ofN-(14-amino-3,6,9,12-tetraoxatetradecyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (200 microliters, 0.020 5 mmol, 1 eq) was addedto JQ-acid (8.0 mg, 0.020 mmol, 1 eq) at room temperature. DIPEA (10.5microliters, 0.060 mmol, 3 eq) and HATU (7.6 mg, 0.020 mmol, 1 eq) wereadded. After 17.5 hours, additional HATU (7.6 mg) and DIPEA (10.5microliters were added) and the mixture was stirred for an additional 5hours. The mixture was diluted with EtOAc and washed with saturatedsodium bicarbonate, water and brine. The combined organic layer wasdried over sodium sulfate, 10 filtered and concentrated under reducedpressure. Purification by column chromatography (ISCO, 4 g silicacolumn, 0-10% MeOH/DCM, 25 minute gradient) gave the desired product(3.66 mg). 1H NMR (500 MHz, Methanol-d₄) 7.79 (dd, J=8.4, 7.4 Hz, 1H),7.51 (d, J=7.3 Hz, 1H), 745 (d, J=7.7 Hz, 2H), 7.43-7.36 (m, 3H), 5.08(ddd, J=12.7, 5.5, 2.2 Hz, 1H), 4.78-4.74 (m, 2H), 4.62 (dd, J=9.1, 5.1Hz, 1H), 3.70-3.51 (m, 16H), 3.50-3.41 (m, SH), 3.27 (dd, J=5.1, (m,1H), 1.69 (s, 3H). LCMS 933.43 (M+H).

Synthetic Example 51: Synthesis of dBET54

A 0.1 M solution ofN-(17-amino-3,6,9,12,15-pentaoxaheptadecyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (200 microliters, 0.020 mmol, 1 eq) was added toJQ-acid (8.0 mg, 0.020 mmol, 1 eq) at room temperature. DIPEA (10.5microliters, 0.060 mmol, 3 eq) and HATU (7.6 mg, 0.020 mmol, 1 eq) wereadded. After 16 hours the mixture was diluted with EtOAc and washed withsaturated sodium bicarbonate, water and brine. The combined organiclayer was dried over sodium sulfate, filtered and concentrated underreduced pressure. Purification by column chromatography (ISCO, 4 gsilica column, 0-10% MeOH/DCM, 25 minute gradient) gave the desiredproduct (6.27 mg, 0.00641 mmol, 32%). ¹H NMR (500 MHz, Methanol-d₄) δ7.81-7.76 (m, 1H), 7.51 (d, J=7.1 Hz, 1H), 7.47-7.38 (m, 5H), 5.09 (dd,J=12.6, 5.5 Hz, 1H), 4.77 (s, 2H), 4.62 (dd, J=8.8, 5.0 Hz, 1H),3.67-3.55 (m, 20H), 3.46 (ddd, J=20.1, 10.2, 4.7 Hz, 5H), 3.28 (d, J=5.1Hz, 1H), 2.91-2.83 (m, 1H), 2.78-2.68 (m, 5H), 2.44 (s, 3H), 2.16-2.10(m, 1H), 1.72-1.66 (m, 3H). LCMS 977.50 (M+H).

Synthetic Example 52: Synthesis of dBET55

A 0.1 M solution ofN-(29-amino-3,6,9,12,15,18,21,24,27-nonaoxanonacosyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (200 microliters, 0.020 mmol, 1 eq) was added toJQ-acid (8.0 mg, 0.020 mmol, 1 eq) at room temperature. DIPEA (10.5microliters, 0.060 mmol, 3 eq) and HATU (7.6 mg, 0.020 mmol, 1 eq) wereadded. After 18 hours the mixture was diluted with EtOAc and washed withsaturated sodium bicarbonate, water and brine. The combined organiclayer was dried over sodium sulfate, filtered and concentrated underreduced pressure. Purification by column chromatography (ISCO, 4 gsilica column, 0-10% MeOH/DCM, 25 minute gradient) gave the desiredproduct (10.55 mg, 0.00914 mmol, 46%). ¹H NMR (500 MHz, Methanol-d₄) δ7.82 (dd, J=8.4, 7.4 Hz, 1H), 7.55 (d, J=7.0 Hz, 1H), 7.49-7.41 (m, 5H),5.13 (dd, J=12.6, 5.5 Hz, 1H), 4.80 (s, 2H), 4.65 (dd, J=9.1, 5.1 Hz,1H), 3.68-3.58 (m, 36H), 3.53-3.44 (m, 5H), 2.94-2.86 (m, 1H), 2.81-2.70(m, 5H), 2.46 (s, 3H), 2.19-2.13 (m, 1H), 1.74-1.69 (m, 3H). LCMS1153.59 (M+H).

Synthetic Example 53: Synthesis of dBET56

A 0.1 M solution ofN-(35-amino-3,6,9,12,15,18,21,24,27,30,33-undecaoxapentatriacontyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate in DMF (200 microliters, 0.020 mmol, 1 eq) was added toJQ-acid (8.0 mg, 0.020 mmol, 1 eq) at room temperature. DIPEA (10.5microliters, 0.060 mmol, 3 eq) and HATU (7.6 mg, 0.020 mmol, 1 eq) wereadded. After 20 hours the mixture was diluted with EtOAc and washed withsaturated sodium bicarbonate, water and brine. The combined organiclayer was dried over sodium sulfate, filtered and concentrated underreduced pressure. Purification by column chromatography (ISCO, 4 gsilica column, 0-10% MeOH/DCM, 25 minute gradient) gave the desiredproduct as an oily residue (9.03 mg, 0.00727 mmol, 36%). ¹H NMR (500MHz, Methanol-d₄) δ 7.81 (dd, J=8.4, 7.4 Hz, 1H), 7.53 (d, J=7.1 Hz,1H), 7.50-7.40 (m, 5H), 5.11 (dd, J=12.6, 5.5 Hz, 1H), 4.78 (s, 2H),4.68 (dd, J=8.6, 5.0 Hz, 1H), 3.69-3.56 (m, 44H), 3.52-3.43 (m, 5H),3.34 (dd, J=7.9, 3.5 Hz, 1H), 2.88 (ddd, J=18.0, 14.0, 5.2 Hz, 1H),2.79-2.68 (m, 5H), 2.46 (s, 3H), 2.17-2.12 (m, 1H), 1.71 (s, 3H). LCMS1241.60 (M+H).

Synthetic Example 54: Synthesis of dBET57 Step 1: Synthesis of2-(2,6-dioxopiperidin-3-yl)-4-fluoroisoindoline-1,3-dione

A solution of 4-fluoroisobenzofuran-1,3-dione (200 mg, 1.20 mmol, 1equiv) in AcOH (4.0 mL, 0.3 M) was added 2,6-dioxopiperidin-3-aminehydrochloride (218 mg, 1.32 mmol, 1.1 equiv) and potassium acetate (366mg, 3.73 mmol, 3.1 equiv). The reaction mixture was heated to 90° C.overnight, whereupon it was diluted with water to 20 mL and cooled onice for 30 min. The resulting slurry was filtered, and the black solidwas purified by flash column chromatography on silica gel (2% MeOH inCH₂Cl₂, R_(f)=0.3) to afford the title compound as a white solid (288mg, 86%). ¹H NMR (500 MHz, DMSO-d₆) δ 11.15 (s, 1H), 7.96 (ddd, J=8.3,7.3, 4.5 Hz, 1H), 7.82-7.71 (m, 2H), 5.17 (dd, J=13.0, 5.4 Hz, 1H), 2.90(ddd, J=17.1, 13.9, 5.4 Hz, 1H), 2.65-2.47 (m, 2H), 2.10-2.04 (m, 1H),MS (ESI) cald for C₁₃H₁₀FN₂O₄ [M+H]⁺ 277.06, found 277.25.

Step 2: Synthesis of tert-butyl(2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)amino)ethyl)carbamate

A stirred solution of2-(2,6-dioxopiperidin-3-yl)-4-fluoroisoindoline-1,3-dione (174 mg, 0.630mmol, 1 equiv) in DMF (6.3 mL, 0.1 M) was added DIPEA (220 μL, 1.26mmol, 2 equiv) and 1-Boc-ethylendiamine (110 μL, 0.693 mmol, 1.1 equiv).The reaction mixture was heated to 90° C. overnight, whereupon it wascooled to room temperature and taken up in EtOAc (30 mL) and water (30mL). The organic layer was washed with brine (3×20 mL), dried overNa₂SO₄ and concentrated in vacuo. The residue was purified by flashcolumn chromatography on silica gel (0-10% MeOH in CH₂Cl₂) to give thetitle compound as a yellow solid (205 mg, 79%). ¹H NMR (500 MHz, CDCl₃)δ 8.08 (bs, 1H), 7.50 (dd, J=8.5, 7.1 Hz, 1H), 7.12 (d, J=7.1 Hz, 1H),6.98 (d, J=8.5 Hz, 1H), 6.39 (t, J=6.1 Hz, 1H), 4.96-4.87 (m, 1H), 4.83(bs, 1H), 3.50-3.41 (m, 2H), 3.41-3.35 (m, 2H), 2.92-2.66 (m, 3H),2.16-2.09 (m, 1H), 1.45 (s, 9H); MS (ESI) cald for C₂₀H₂₅N₄O₆ [M+H]⁺417.18, found 417.58.

Step 3: Synthesis of2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)amino)ethan-1-aminium2,2,2-trifluoroacetate

A stirred solution of tert-butyl(2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)amino)ethyl)carbamate(205 mg, 0.492 mmol, 1 equiv) in dichloromethane (2.25 mL) was addedtrifluoroacetic acid (0.250 mL). The reaction mixture was stirred atroom temperature for 4 h, whereupon the volatiles were removed in vacuo.The title compound was obtained as a yellow solid (226 mg, >95%), thatwas used without further purification. ¹H NMR (500 MHz, MeOD) δ 7.64 (d,J=1.4 Hz, 1H), 7.27-7.05 (m, 2H), 5.10 (dd, J=12.5, 5.5 Hz, 1H), 3.70(t, J=6.0 Hz, 2H), 3.50-3.42 (m, 2H), 3.22 (t, J=6.0 Hz, 1H), 2.93-2.85(m, 1H), 2.80-2.69 (m, 2H), 2.17-2.10 (m, 1H); MS (ESI) cald forC₁₅H₁₇N₄O₄ [M+H]⁺ 317.12, found 317.53.

Step 2: Synthesis of dBET57

JQ-acid (8.0 mg, 0.0200 mmol, 1 eq) and2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)amino)ethan-1-aminium2,2,2-trifluoroacetate (8.6 mg, 0.0200 mmol, 1 equiv) were dissolved inDMF (0.200 mL, 0.1 M) at room temperature. DIPEA (17.4 μL, 0.100 mmol, 5equiv) and HATU (7.59 mg, 0.0200 mmol, 1 equiv) were then added and themixture was stirred at room temperature overnight. The reaction mixturewas taken up in EtOAc (15 mL), and washed with satd. NaHCO₃(aq) (15 mL),water (15 mL) and brine (3×15 mL). The organic layer was dried overNa₂SO₄ and concentrated in vacuo. The residue was purified by flashcolumn chromatography on silica gel (0-10% MeOH in CH₂Cl₂, R_(f)=0.3(10% MeOH in CH₂Cl₂)) to give the title compound as a bright yellowsolid (11.2 mg, 80%). ¹H NMR (400 MHz, CDCl₃) δ 8.49 (bs, 0.6H), 8.39(bs, 0.4H), 7.51-7.43 (m, 1H), 7.38 (d, J=7.8 Hz, 2H), 7.29 (dd, J=8.8,1.7 Hz, 2H), 7.07 (dd, J=7.1, 4.9 Hz, 1H), 6.97 (dd, J=8.6, 4.9 Hz, 1H),6.48 (t, J=5.9 Hz, 1H), 6.40 (t, J=5.8 Hz, 0.6H), 4.91-4.82 (m, 0.4H),4.65-4.60 (m, 1H), 3.62-3.38 (m, 6H), 2.87-2.64 (m, 3H), 2.63 (s, 3H),2.40 (s, 6H), 2.12-2.04 (m, 1H), 1.67 (s, 3H), rotamers; MS (ESI) calcdfor C₃₄H₃₂ClN₈O₅S [M+H]⁺ 700.19, found 700.34.

Synthetic Example 55: Synthesis of dGR1

Synthetic Example 56: Synthesis of dGR2

Synthetic Example 57: Synthesis of dGR3

Synthetic Example 58: Synthesis of dFKBP-1

(1) Synthesis of SLF-succinate

SLF (25 mg, 2.5 mL of a 10 mg/mL solution in MeOAc, 0.0477 mmol, 1 eq)was combined with DMF (0.48 mL, 0.1 M) and succinic anhydride (7.2 mg,0.0715 mmol, 1.5 eq) and stirred at room temperature for 24 hours. Lowconversion was observed and the mixture was placed under a stream of N2to remove the MeOAc. An additional 0.48 mL of DMF was added, along withan additional 7.2 mg succinic anhydride and DMAP (5.8 mg, 0.0477 mmol, 1eq). The mixture was then stirred for an additional 24 hours beforebeing purified by preparative HPLC to give SLF-succinate as a yellow oil(24.06 mg, 0.0385 mmol, 81%).

¹H NMR (400 MHz, Methanol-d₄) δ 7.62 (d, J=10.7 Hz, 1H), 7.44 (d, J=8.0Hz, 1H), 7.26 (td, J=7.9, 2.7 Hz, 1H), 7.07-6.97 (m, 1H), 6.80 (dd,J=8.1, 2.1 Hz, 1H), 6.74-6.66 (m, 2H), 5.73 (dd, J=8.1, 5.5 Hz, 1H),5.23 (d, J=4.8 Hz, 1H), 3.83 (s, 3H), 3.81 (s, 3H), 3.39-3.29 (m, 4H),3.21 (td, J=13.2, 3.0 Hz, 1H), 2.68-2.50 (m, 5H), 2.37-2.19 (m, 2H),2.12-2.02 (m, 1H), 1.79-1.61 (m, 4H), 1.49-1.30 (m, 2H), 1.27-1.05 (m,6H), 0.82 (dt, J=41.2, 7.5 Hz, 3H). LCMS 624.72 (M+H).

(2) Synthesis of dFKBP-1

N-(4-aminobutyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate (9.9 mg, 0.0192 mmol, 1 eq) was added to SLFsuccinate(11.98 mg, 0.0192 mmol, 1 eq) as a solution in 0.192 mL DMF (0.1 M).DIPEA (10.0 microliters, 0.0575 mmol, 3 eq) was added, followed by HATU(7.3 mg, 0.0192 mmol, 1 eq). The mixture was stirred for 17 hours, thendiluted with MeOH and purified by preparative HPLC to give dFKBP-1 (7.7mg, 0.00763 mmol, 40%) as a yellow solid.

¹H NMR (400 MHz, Methanol-d₄) δ 7.81 (s, 1H), 7.77-7.70 (m, 1H),7.55-7.49 (m, 2H), 7.26 (dd, J=8.0, 5.3 Hz, 2H), 7.05-6.99 (m, 1H), 6.77(d, J=8.8 Hz, 1H), 6.66 (d, J=6.8 Hz, 2H), 5.77-5.72 (m, 1H), 5.24 (d,J=4.8 Hz, 1H), 4.99 (dd, J=12.3, 5.7 Hz, 1H), 4.68-4.59 (m, 2H), 3.82(s, 3H), 3.81 (s, 3H), 3.32 (dt, J=3.3, 1.6 Hz, 4H), 3.26-3.14 (m, 3H),2.79 (dd, J=18.9, 10.2 Hz, 3H), 2.64-2.48 (m, 5H), 2.34 (d, J=14.4 Hz,1H), 2.22 (d, J=9.2 Hz, 1H), 2.14-2.02 (m, 2H), 1.78-1.49 (m, 9H),1.43-1.30 (m, 2H), 1.20-1.04 (m, 6H), 0.90-0.76 (m, 3H). 13C NMR (100MHz, cd₃od) δ 208.51, 173.27, 172.64, 171.63, 169.93, 169.51, 168.04,167.69, 167.09, 166.71, 154.92, 149.05, 147.48, 140.76, 138.89, 137.48,133.91, 133.67, 129.36, 122.19, 120.61, 120.54, 119.82, 118.41, 118.12,117.79, 112.12, 111.76, 68.54, 56.10, 55.98, 51.67, 46.94, 44.57, 39.32,39.01, 38.23, 32.64, 31.55, 31.43, 26.68, 26.64, 25.08, 23.52, 23.21,22.85, 21.27, 8.76. LCMS 1009.66 (M+H).

Synthetic Example 59: Synthesis of dFKBP-2

(1) Synthesis of tert-butyl(1-chloro-2-oxo-7,10,13-trioxa-3-azahexadecan-16-yl)carbamate

tert-butyl (3-(2-(2-(3-aminopropoxy)ethoxy)ethoxy)propyl)carbamate (1.0g, 3.12 mmol, 1 eq) was dissolved in THF (31 mL, 0.1 M). DIPEA (0.543mL, 3.12 mmol, 1 eq) was added and the solution was cooled to 0° C.Chloroacetyl chloride (0.273 mL, 3.43 mmol, 1.1 eq) was added and themixture was warmed slowly to room temperature. After 24 hours, themixture was diluted with EtOAc and washed with saturated sodiumbicarbonate, water then brine. The organic layer was dried over sodiumsulfate, filtered and condensed to give a yellow oil (1.416 g) that wascarried forward without further purification.

¹H NMR (400 MHz, Chloroform-d) δ 7.24 (s, 1H), 5.00 (s, 1H), 3.98-3.89(m, 2H), 3.54 (dddt, J=17.0, 11.2, 5.9, 2.2 Hz, 10H), 3.47-3.40 (m, 2H),3.37-3.31 (m, 2H), 3.17-3.07 (m, 2H), 1.79-1.70 (m, 2H), 1.67 (p, J=6.1Hz, 2H), 1.35 (s, 9H). ¹³C NMR (100 MHz, cdcl₃) δ 165.83, 155.97, 78.75,70.49, 70.47, 70.38, 70.30, 70.14, 69.48, 42.61, 38.62, 38.44, 29.62,28.59, 28.40. LCMS 397.37 (M+H).

(2) Synthesis of dimethyl3-((2,2-dimethyl-4,20-dioxo-3,9,12,15-tetraoxa-5,19-diazahenicosan-21-yl)oxy)phthalate

tert-butyl (1-chloro-2-oxo-7,10,13-trioxa-3-azahexadecan-16-yl)carbamate(1.41 g, 3.12 mmol, 1 eq) was dissolved in MeCN (32 mL, 0.1 M). Dimethyl3-hydroxyphthalate (0.721 g, 3.43 mmol, 1.1 eq) and cesium carbonate(2.80 g, 8.58 mmol, 2.75 eq) were added. The flask was fitted with areflux condenser and heated to 80° C. for 19 hours. The mixture wascooled to room temperature and diluted water and extracted once withchloroform and twice with EtOAc. The combined organic layers were driedover sodium sulfate, filtered and concentrated under reduced pressure.The crude material was purified by column chromatography (ISCO, 24 gsilica column, 0-15% MeOH/DCM 22 minute gradient) to give a yellow oil(1.5892 g, 2.78 mmol, 89% over two steps).

¹H NMR (400 MHz, Chloroform-d) δ 7.52 (d, J=7.8 Hz, 1H), 7.35 (t, J=8.1Hz, 1H), 7.04 (d, J=8.3 Hz, 1H), 7.00 (t, J=5.3 Hz, 1H), 5.06 (s, 1H),4.46 (s, 2H), 3.83 (s, 3H), 3.78 (s, 3H), 3.47 (ddd, J=14.9, 5.5, 2.8Hz, 8H), 3.39 (dt, J=9.4, 6.0 Hz, 4H), 3.29 (q, J=6.5 Hz, 2H), 3.09 (d,J=6.0 Hz, 2H), 1.70 (p, J=6.5 Hz, 2H), 1.63 (p, J=6.3 Hz, 2H), 1.31 (s,9H). ¹³C NMR (100 MHz, cdcl₃) δ 167.68, 167.36, 165.45, 155.93, 154.41,130.87, 129.60, 125.01, 123.20, 117.06, 78.60, 70.40, 70.17, 70.06,69.39, 68.67, 68.25, 52.77, 52.57, 38.38, 36.58, 29.55, 29.20, 28.34.LCMS 571.47 (M+H).

(3) Synthesis ofN-(3-(2-(2-(3-aminopropoxy)ethoxy)ethoxy)propyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate

Dimethyl3-((2,2-dimethyl-4,20-dioxo-3,9,12,15-tetraoxa-5,19-diazahenicosan-21-yl)oxy)phthalate(1.589 g, 2.78 mmol, 1 eq) was dissolved in EtOH (14 mL, 0.2 M). Aqueous3M NaOH (2.8 mL, 8.34 mmol, 3 eq) was added and the mixture was heatedto 80° C. for 22 hours. The mixture was then cooled to room temperature,diluted with 50 mL DCM and 20 mL 0.5 M HCl. The layers were separatedand the organic layer was washed with 25 mL water. The aqueous layerswere combined and extracted three times with 50 mL chloroform. Thecombined organic layers were dried over sodium sulfate, filtered andcondensed to give 1.53 g of material that was carried forward withoutfurther purification. LCMS 553.44.

The resultant material (1.53 g) and 3-aminopiperidine-2,6-dionehydrochloride (0.480 g, 2.92 mmol, 1 eq) were dissolved in pyridine(11.7 mL, 0.25 M) and heated to 110° C. for 17 hours. The mixture wascooled to room temperature and concentrated under reduced pressure togive crude tert-butyl(1-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)-2-oxo-7,10,13-trioxa-3-azahexadecan-16-yl)carbamateas a black sludge (3.1491 g) that was carried forward without furtherpurification. LCMS 635.47.

The crude tert-butyl(1-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)-2-oxo-7,10,13-trioxa-3-azahexadecan-16-yl)carbamate(3.15 g) was dissolved in TFA (20 mL) and heated to 50° C. for 2.5hours. The mixture was cooled to room temperature, diluted with MeOH andconcentrated under reduced pressure. The material was purified bypreparative HPLC to giveN-(3-(2-(2-(3-aminopropoxy)ethoxy)ethoxy)propyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate (1.2438 g, 1.9598 mmol, 71% over 3 steps) as a dark redoil.

¹H NMR (400 MHz, Methanol-d₄) δ 7.77 (dd, J=8.3, 7.5 Hz, 1H), 7.49 (d,J=7.3 Hz, 1H), 7.40 (d, J=8.5 Hz, 1H), 5.12 (dd, J=12.8, 5.5 Hz, 1H),4.75 (s, 2H), 3.68-3.51 (m, 12H), 3.40 (t, J=6.8 Hz, 2H), 3.10 (t, J=6.4Hz, 2H), 2.94-2.68 (m, 3H), 2.16 (dtd, J=12.6, 5.4, 2.5 Hz, 1H), 1.92(p, J=6.1 Hz, 2H), 1.86-1.77 (m, 2H). ¹³C NMR (100 MHz, cd₃od) a 173.17,169.97, 168.48, 166.87, 166.30, 154.82, 136.89, 133.41, 120.29, 117.67,116.58, 69.96, 69.68, 69.60, 68.87, 68.12, 67.92, 49.19, 38.62, 36.14,30.80, 28.92, 26.63, 22.22. LCMS 536.41 (M+H).

(4) Synthesis of dFKBP-2

N-(3-(2-(2-(3-aminopropoxy)ethoxy)ethoxy)propyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate(12.5 mg, 0.0193 mmol, 1 eq) was added to SLF-succinate(12.08 mg, 0.0193 mmol, 1 eq) as a solution in 0.193 mL in DMF (0.1 M).DIPEA (10.1 microliters, 0.0580 mmol, 3 eq) and HATU (7.3 mg, 0.0193mmol, 1 eq) were added and the mixture was stirred for 19 hours. Themixture was then diluted with MeOH and purified by preparative HPLC togive dFKBP-2 (9.34 mg, 0.00818 mmol, 42%) as a yellow oil.

¹H NMR (400 MHz, 50% MeOD/Chloroform-d) δ 7.76-7.70 (m, 1H), 7.58-7.45(m, 3H), 7.26 (t, J=8.2 Hz, 2H), 7.05-6.98 (m, 1H), 6.77 (d, J=7.9 Hz,1H), 6.71-6.63 (m, 2H), 5.73 (dd, J=8.1, 5.6 Hz, 1H), 5.23 (d, J=5.4 Hz,1H), 5.03-4.95 (m, 1H), 4.64 (s, 2H), 3.82 (s, 3H), 3.80 (s, 3H),3.62-3.52 (m, 8H), 3.47 (t, J=6.1 Hz, 2H), 3.44-3.33 (m, 3H), 3.27-3.14(m, 3H), 2.84-2.70 (m, 3H), 2.64-2.47 (m, 6H), 2.34 (d, J=14.1 Hz, 1H),2.24 (dd, J=14.3, 9.3 Hz, 2H), 2.13-2.00 (m, 2H), 1.83 (p, J=6.3 Hz,2H), 1.67 (dtd, J=38.4, 16.8, 14.8, 7.0 Hz, 7H), 1.51-1.26 (m, 3H),1.22-1.05 (m, 6H), 0.80 (dt, J=39.8, 7.5 Hz, 3H). ¹³C NMR (100 MHz,cdcl₃) δ 208.64, 173.39, 173.01, 171.76, 170.11, 169.62, 168.24, 167.92,167.36, 166.69, 155.02, 149.23, 147.66, 140.94, 139.18, 137.57, 134.09,133.91, 129.49, 122.32, 120.75, 120.52, 119.93, 118.42, 117.75, 112.33,111.98, 70.77, 70.51, 70.40, 69.45, 69.04, 68.48, 56.20, 56.10, 51.88,47.09, 44.78, 38.40, 37.48, 36.91, 32.80, 32.71, 31.70, 31.59, 31.55,29.53, 29.30, 26.77, 25.22, 23.63, 23.33, 22.98, 21.43. LCMS 1141.71(M+H).

Synthetic Example 60: Synthesis of dFKBP-3

A 0.1 M solution ofN-(4-aminobutyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate (0.233 mL, 0.0233 mmol, 1 eq) was added to2-(3-((R)-3-(3,4-dimethoxyphenyl)-1-(((S)-1-(3,3-dimethyl-2-oxopentanoyl)pyrrolidine-2-carbonyl)oxy)propyl)phenoxy)aceticacid (13.3 mg, 0.0233 mmol, 1 eq). DIPEA (12.2 microliters, 0.0700 mmol,3 eq) was added, followed by HATU (8.9 mg, 0.0233 mmol, 1 eq). Themixture was stirred for 23 hours, then diluted with MeOH and purified bypreparative HPLC to give a white solid (10.72 mg mg, 0.0112 mmol, 48%).

¹H NMR (400 MHz, Methanol-d₄) δ 7.79-7.74 (m, 1H), 7.52 (d, J=7.4 Hz,1H), 7.33 (d, J=8.4 Hz, 1H), 7.26 (t, J=8.1 Hz, 1H), 6.97-6.90 (m, 2H),6.89-6.84 (m, 1H), 6.79 (dd, J=8.2, 1.9 Hz, 1H), 6.73-6.64 (m, 2H),5.73-5.65 (m, 1H), 5.07-4.99 (m, 1H), 4.67 (s, 2H), 4.57-4.51 (m, 1H),4.48 (dd, J=5.7, 2.5 Hz, 2H), 3.82 (d, J=1.9 Hz, 3H), 3.80 (s, 3H),3.66-3.39 (m, 3H), 2.88-2.48 (m, 6H), 2.42-1.87 (m, 9H), 1.73-1.51 (m,6H), 1.19-0.92 (m, 6H), 0.75 (dt, J=56.7, 7.5 Hz, 3H). LCMS 954.52(M+H).

Example 61: Synthesis of dFKBP-4

A 0.1 M solution ofN-(4-aminobutyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate (0.182 mL, 0.0182 mmol, 1 eq) was added to2-(3-((R)-3-(3,4-dimethoxyphenyl)-1-(((S)-1-(3,3-dimethyl-2-oxopentanoyl)piperidine-2-carbonyl)oxy)propyl)phenoxy)aceticacid (10.6 mg, 0.0182 mmol, 1 eq). DIPEA (9.5 microliters, 0.0545 mmol,3 eq) was added, followed by HATU (6.9 mg, 0.0182 mmol, 1 eq). Themixture was stirred for 26 hours, then diluted with MeOH and purified bypreparative HPLC to give a white solid (9.74 mg, 0.01006 mmol, 55%).

¹H NMR (400 MHz, Methanol-d₄) δ 7.75 (dd, J=8.3, 7.4 Hz, 1H), 7.53 (d,J=2.3 Hz, 1H), 7.33-7.25 (m, 2H), 7.00-6.84 (m, 3H), 6.79 (dd, J=8.1,2.5 Hz, 1H), 6.72-6.65 (m, 2H), 5.75-5.70 (m, 1H), 5.23 (d, J=4.9 Hz,1H), 5.05-4.96 (m, 1H), 4.66 (s, 2H), 4.46 (s, 2H), 3.82 (s, 3H), 3.81(s, 3H), 3.39-3.32 (m, 4H), 3.20-3.12 (m, 1H), 2.82-2.69 (m, 3H),2.62-2.49 (m, 2H), 2.37-2.00 (m, 5H), 1.78-1.30 (m, 11H), 1.24-1.08 (m,6H), 0.81 (dt, J=32.9, 7.5 Hz, 3H). LCMS 968.55 (M+H).

Synthetic Example 62: Synthesis of dFKBP-5

A 0.1 M solution ofN-(4-aminobutyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate (0.205 mL, 0.0205 mmol, 1 eq) was added to2-(3-((R)-3-(3,4-dimethoxyphenyl)-1-(((S)-1-(2-phenylacetyl)piperidine-2-carbonyl)oxy)propyl)phenoxy)aceticacid (11.8 mg, 0.0205 mmol, 1 eq). DIPEA (10.7 microliters, 0.0615 mmol,3 eq) was added, followed by HATU (7.8 mg, 0.0205 mmol, 1 eq). Themixture was stirred for 29 hours, then diluted with MeOH and purified bypreparative HPLC to give a white solid (10.62 mg, 0.01106 mmol, 54%).

¹H NMR (400 MHz, Methanol-d₄) δ 7.77-7.72 (m, 1H), 7.52 (s, 1H),7.31-7.11 (m, 7H), 6.92-6.77 (m, 4H), 6.68-6.62 (m, 2H), 5.70-5.64 (m,1H), 5.38 (d, J=3.8 Hz, 1H), 4.99 (d, J=4.6 Hz, 1H), 4.65 (s, 2H),4.45-4.39 (m, 2H), 3.80 (dd, J=6.7, 2.4 Hz, 8H), 3.13-3.03 (m, 1H),2.83-2.68 (m, 3H), 2.63-2.45 (m, 3H), 2.34-1.93 (m, 6H), 1.71-1.52 (m,7H), 1.34-1.20 (m, 3H). LCMS 960.54 (M+H).

Synthetic Example 63: Synthesis of dFKBP-6

N-(4-aminobutyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate(11.9 mg, 0.0231 mmol, 1 eq) is added to2-(3-((R)-3-(3,4-dimethoxyphenyl)-1-(((S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carbonyl)oxy)propyl)phenoxy)aceticacid (16.0 mg, 0.0231 mmol, 1 eq) as a solution in 0.231 mL DMF (0.1 M).DIPEA (12.1 microliters, 0.0692 mmol, 3 eq) and HATU (8.8 mg, 0.0231mmol, 1 eq) are added and the mixture is stirred for 21 hours. Themixture is diluted with EtOAc and washed with saturated sodiumbicarbonate, water and brine. The organic layer is dried over sodiumsulfate, filtered and concentrated under reduced pressure. The crudematerial is purified by column chromatography.

The above synthetic scheme can also be used to provide the analogousortho or para bonding configuration in the dFKBP structures herein bychoice of starting material, as illustrated below. Any of thesepositional isomers can be used in the present invention to degrade FKBP.For example:

Similarly use of

Synthetic Example 64: Synthesis of dFKBP-7

N-(3-(2-(2-(3-aminopropoxy)ethoxy)ethoxy)propyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoracetate (12.3 mg, 0.0189 mmol, 1 eq) is added to2-(3-((R)-3-(3,4-dimethoxyphenyl)-1-(((S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carbonyl)oxy)propyl)phenoxy)acetic acid (13.1 mg, 0.0189mmol, 1 eq) as a solution in 0.189 mL DMF (0.1 M). DIPEA (9.9microliters, 0.0566 mmol, 3 eq) and HATU (7.2 mg, 0.0189 mmol, 1 eq) areadded and the mixture is stirred for 17 hours. The mixture is dilutedwith EtOAc and washed with saturated sodium bicarbonate, water andbrine. The organic layer is dried over sodium sulfate, filtered andconcentrated under reduced pressure. The crude material is purified bycolumn chromatography.

Synthetic Example 65: Synthesis of dFKBP-8

N-(6-aminohexyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoracetate (12.7 mg, 0.0233 mmol, 1.3 eq) is added to2-(3-((R)-3-(3,4-dimethoxyphenyl)-1-(((S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carbonyl)oxy)propyl)phenoxy)aceticacid (12.4 mg, 0.0179 mmol, 1 eq) as a solution in 0.233 mL DMF (0.1 M).DIPEA (9.3 microliters, 0.0537 mmol, 3 eq) and HATU (6.8 mg, 0.0179mmol, 1 eq) are added and the mixture is stirred for 22 hours. Themixture is diluted with EtOAc and washed with saturated sodiumbicarbonate, water and brine. The organic layer is dried over sodiumsulfate, filtered and concentrated under reduced pressure. The crudematerial is purified by column chromatography.

Synthetic Example 66: Synthesis of dFKBP-9

N-(8-aminooctyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate (10.4 mg, 0.0181 mmol, 1 eq) is added to2-(3-((R)-3-(3,4-dimethoxyphenyl)-1-(((S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carbonyl)oxy)propyl)phenoxy)aceticacid (12.5 mg, 0.0181 mmol, 1 eq) as a solution in 0.181 mL DMF (0.1 M).DIPEA (9.5 microliters, 0.0543 mmol, 3 eq) and HATU (6.9 mg, 0.0181mmol, 1 eq) are added and the mixture is stirred for 22 hours. Themixture is diluted with EtOAc and washed with saturated sodiumbicarbonate, water and brine. The organic layer is dried over sodiumsulfate, filtered and concentrated under reduced pressure. The crudematerial is purified by column chromatography.

Synthetic Example 67: Synthesis of dFKBP

X₂

FKBP*-acid (14.0 mg, 0.0202 mmol, 1 eq) and2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)amino)ethan-1-aminium2,2,2-trifluoroacetate (8.7 mg, 0.0202 mmol, 1 equiv) are dissolved inDMF (0.202 mL, 0.1 M) at room temperature. DIPEA (17.6 OL, 0.101 mmol, 5equiv) and HATU (7.6 mg, 0.0200 mmol, 1 equiv) are then added and themixture is stirred at room temperature overnight. The reaction mixtureis taken up in EtOAc (15 mL), and washed with satd. NaHCO₃(aq) (15 mL),water (15 mL) and brine (3×15 mL). The organic layer is dried overNa₂SO₄ and concentrated in vacuo. The crude material is purified bycolumn chromatography.

Synthetic Example 68: Synthesis of diaminoethyl-acetyl-O-thalidomidetrifluoroacetate

(1) Synthesis of tert-Butyl (2-(2-chloroacetamido)ethyl)carbamate

tert-butyl (2-aminoethyl)carbamate (0.40 mL, 2.5 mmol, 1 eq) wasdissolved in THF (25 mL, 0.1 M) and DIPEA (0.44 mL, 2.5 mmol, 1 eq) at0° C. Chloroacetyl chloride (0.21 mL, 2.75 mmol, 1.1 eq) was added andthe mixture was allowed to warm to room temperature. After 22 hours, themixture was diluted with EtOAc and washed with saturated sodiumbicarbonate, water and brine. The organic layer was dried with sodiumsulfate, filtered and concentrated under reduced pressure to give awhite solid (0.66 g, quantitative yield) that carried forward to thenext step without further purification. ¹H NMR (400 MHz, Chloroform-d) δ7.16 (s, 1H), 4.83 (s, 1H), 4.04 (s, 2H), 3.42 (q, J=5.4 Hz, 2H), 3.32(q, J=5.6 Hz, 2H), 1.45 (s, 9H). LCMS 237.30 (M+H).

(2) Synthesis of dimethyl3-(2-((2-((tert-butoxycarbonyl)amino)ethyl)amino)-2-oxoethoxy)phthalate

tert-butyl (2-(2-chloroacetamido)ethyl)carbamate (0.66 g, 1 eq) wasdissolved in MeCN (17 mL, 0.15 M). Dimethyl 3-hydroxyphthalate (0.578 g,2.75 mmol, 1.1 eq) and cesium carbonate (2.24 g, 6.88 mmol, 2.75 eq)were then added. The flask was fitted with a reflux condenser and heatedto 80° C. for 32 hours. The mixture was then cooled to room temperature,diluted with EtOAc and washed three times with water. The organic layerwas dried over sodium sulfate, filtered and concentrated under reducedpressure. Purification by column chromatography (ISCO, 4 g silicacolumn, 0-15% MeOH/DCM over a 15 minute gradient) gave a yellow solid(0.394 g, 0.960 mmol, 38% over 2 steps). ¹H NMR (400 MHz, Chloroform-d)δ 7.65-7.56 (m, 1H), 7.50-7.41 (m, 1H), 7.27 (s, 1H), 7.11 (dd, J=8.4,4.1 Hz, 2H), 5.17 (s, 1H), 4.57 (d, J=6.3 Hz, 2H), 3.94 (s, 2H), 3.88(s, 2H), 3.40 (p, J=5.8 Hz, 4H), 3.32-3.19 (m, 4H), 1.39 (d, J=5.7 Hz,13H). ¹³C NMR (100 MHz, cdcl₃) δ 168.37, 168.23, 165.73, 156.13, 154.71,131.24, 130.09, 124.85, 123.49, 117.24, 79.42, 68.48, 53.22, 52.83,40.43, 39.54, 28.44. LCMS 411.45 (M+H).

(3) Synthesis of diaminoethyl-acetyl-O-thalidomide trifluoroacetate

Dimethyl3-(2-((2-((tert-butoxycarbonyl)amino)ethyl)amino)-2-oxoethoxy)phthalate(0.39 g, 0.970 mmol, 1 eq) was dissolved in EtOH (9.7 mL, 0.1 M).Aqueous 3M NaOH (0.97 mL, 2.91 mmol, 3 eq) was added and the mixture washeated to 80° C. for 3 hours. The mixture was cooled to roomtemperature, diluted with 50 mL DCM, 5 mL 1 M HCl and 20 mL water. Thelayers were separated and the organic layer was washed with 20 mL water.The combined aqueous layers were then extracted 3 times with 50 mLchloroform. The combined organic layers were dried over sodium sulfate,filtered and concentrated under reduced pressure to give a yellow solid(0.226 g) that was carried forward without further purification. LCMS383.36.

The resultant yellow solid (0.226 g) and 3-aminopiperidine-2,6-dionehydrochloride (0.102 g, 0.6197 mmol, 1 eq) were dissolved in pyridine(6.2 mL, 0.1 M) and heated to 110° C. for 16 hours. The mixture wascooled to room temperature and concentrated under reduced pressure togive tert-butyl(2-(2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamido)ethyl)carbamateas a poorly soluble black tar (0.663 g) which was carried forwardwithout purification (due to poor solubility). LCMS 475.42 (M+H).

The crude tert-butyl(2-(2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamido)ethyl)carbamatewas dissolved in TFA (10 mL) and heated to 50° C. for 3.5 hours, thenconcentrated under reduced pressure. Purification by preparative HPLCgave a red oil (176.7 mg, 0.362 mmol, 37% over 3 steps). ¹H NMR (400MHz, Methanol-d₄) δ 7.85-7.76 (m, 1H), 7.57-7.50 (m, 1H), 7.48-7.41 (m,1H), 5.13 (dd, J=12.6, 5.5 Hz, 1H), 4.81 (s, 2H), 3.62 (td, J=5.6, 1.8Hz, 2H), 3.14 (t, J=5.8 Hz, 2H), 2.97 (s, 1H), 2.80-2.66 (m, 2H), 2.15(dddd, J=10.1, 8.0, 5.8, 2.8 Hz, 1H). ¹³C NMR (100 MHz, cd₃od) δ 173.09,170.00, 169.99, 166.78, 166.62, 154.93, 136.88, 133.46, 120.71, 117.93,116.77, 68.29, 49.17, 39.37, 38.60, 30.73, 22.19. LCMS 375.30 (M+H forfree base).

Synthetic Example 69: Synthesis of diaminobutyl-acetyl-O-thalidomidetrifluoroacetate

Diaminobutyl-acetyl-O-thalidomide trifluoroacetate was preparedaccording to the procedure in Fischer et al. Nature, 2014, 512, 49-53.

Synthetic Example 70: Synthesis of diaminohexyl-acetyl-O-thalidomidetrifluoroacetate

(1) Synthesis of tert-butyl (6-(2-chloroacetamido)hexyl)carbamate

tert-butyl (6-aminohexyl)carbamate (0.224 mL, 1.0 mmol, 1 eq) wasdissolved in THF (10 mL, 0.1 M). DIPEA (0.17 mL, 1.0 mmol, 1 eq) wasadded and the mixture was cooled to 0° C. Chloroacetyl chloride (88microliters, 1.1 mmol, 1.1 eq) was added and the mixture was warmed toroom temperature and stirred for 18 hours. The mixture was then dilutedwith EtOAc and washed with saturated sodium bicarbonate, water andbrine. The organic layer was dried over sodium sulfate, filtered andconcentrated under reduced pressure to give a white solid (0.2691 g,0.919 mmol, 92%). ¹H NMR (400 MHz, Chloroform-d) δ 6.60 (s, 1H), 4.51(s, 1H), 4.05 (s, 2H), 3.30 (q, J=6.9 Hz, 2H), 3.11 (d, J=6.7 Hz, 2H),1.57-1.46 (m, 4H), 1.44 (s, 9H), 1.38-1.32 (m, 4H). LCMS 293.39 (M+H).

(2) Synthesis of dimethyl3-(2-((6-((tert-butoxycarbonyl)amino)hexyl)amino)-2-oxoethoxy)phthalate

tert-butyl (6-(2-chloroacetamido)hexyl)carbamate (0.2691 g, 0.919 mmol,1 eq) was dissolved in MeCN (9.2 mL, 0.1 M). Dimethyl 3-hydroxyphthalate(0.212 g, 1.01 mmol, 1.1 eq) and cesium carbonate (0.823 g, 2.53 mmol,2.75 eq) were added. The flask was fitted with a reflux condenser andheated to 80° C. for 14 hours. The mixture was cooled to roomtemperature and diluted with EtOAc, washed three times with water andback extracted once with EtOAc. The combined organic layers were driedover sodium sulfate, filtered and concentrated under reduced pressure.The crude material was purified by column chromatography (ISCO, 12 gsilica column, 0-15% MeOH/DCM 15 minute gradient) to give a yellow oil(0.304 g, 0.651 mmol, 71%). ¹H NMR (400 MHz, Chloroform-d) δ 7.66-7.58(m, 1H), 7.44 (td, J=8.2, 1.6 Hz, 1H), 7.15-7.08 (m, 1H), 6.96 (s, 1H),4.56 (s, 2H), 3.92 (t, J=1.6 Hz, 3H), 3.88 (t, J=1.6 Hz, 3H), 3.27 (q,J=6.9 Hz, 2H), 3.10-3.00 (m, 2H), 1.41 (s, 13H), 1.33-1.22 (m, 4H). ¹³CNMR (100 MHz, cdcl₃) δ 167.97, 167.37, 165.58, 155.95, 154.37, 130.97,129.74, 124.94, 123.26, 116.81, 78.96, 68.04, 52.89, 52.87, 52.69,52.67, 40.41, 38.96, 29.88, 29.13, 28.39, 26.33, 26.30. LCMS 467.49.

(3) Synthesis of diaminohexyl-acetyl-O-thalidomide trifluoroacetate

Dimethyl3-(2-((6-((tert-butoxycarbonyl)amino)hexyl)amino)-2-oxoethoxy)phthalate(0.304 g, 0.651 mmol, 1 eq) was dissolved in EtOH (6.5 mL, 0.1 M).Aqueous 3M NaOH (0.65 mL, 1.953 mmol, 3 eq) was added and the mixturewas heated to 80° C. for 18 hours. The mixture was cooled to roomtemperature and diluted with 50 mL DCM and 10 mL 0.5 M HCl. The layerswere separated and the organic layer was washed with 20 mL water. Thecombined aqueous layers were then extracted 3 times with chloroform. Thecombined organic layers were dried over sodium sulfate, filtered andconcentrated under reduced pressure to give a yellow foam (0.290 g) thatwas carried forward without further purification. LCMS 439.47.

The resultant yellow solid (0.290 g) and 3-aminopiperidine-2,6-dionehydrochloride (0.113 g, 0.69 mmol, 1 eq) were dissolved in pyridine (6.9mL, 0.1 M) and heated to 110° C. for 17 hours. The mixture was cooled toroom temperature and concentrated under reduced pressure to givetert-butyl(6-(2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamido)hexyl)carbamateas a black solid (0.4216 g) which was carried forward withoutpurification (due to poor solubility). LCMS 531.41 (M+H).

The crude tert-butyl(6-(2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamido)hexyl)carbamate(0.4216 g) was dissolved in TFA (10 mL) and heated to 50° C. for 2hours. The mixture was concentrated under reduced pressure, thenconcentrated under reduced pressure. Purification by preparative HPLCgave a brown solid (379.2 mg). ¹H NMR (400 MHz, Methanol-d₄) δ 7.79 (dd,J=8.4, 7.4 Hz, 1H), 7.52 (d, J=7.2 Hz, 1H), 7.42 (d, J=8.4 Hz, 1H), 5.13(dd, J=12.6, 5.5 Hz, 1H), 4.75 (s, 2H), 3.32 (t, J=7.6 Hz, 2H),2.96-2.89 (m, 2H), 2.89-2.65 (m, 3H), 2.16 (ddt, J=10.4, 5.4, 2.9 Hz,1H), 1.63 (dp, J=20.6, 7.1 Hz, 4H), 1.51-1.34 (m, 4H). ¹³C NMR (100 MHz,cd₃od) δ 174.57, 171.42, 169.90, 168.24, 167.79, 156.23, 138.23, 134.87,121.69, 119.22, 117.98, 69.36, 50.53, 40.64, 39.91, 32.14, 30.01, 28.44,27.23, 26.96, 23.63. LCMS 431.37 (M+H).

Synthetic Example 71: Synthesis of diaminooctyl-acetyl-O-thalidomidetrifluoroacetate

(1) Synthesis of tert-Butyl (8-(2-chloroacetamido)octyl)carbamate

Octane-1,8-diamine (1.65 g, 11.45 mmol, 5 eq) was dissolved inchloroform (50 mL). A solution of di-tert-butyl dicarbonate (0.54 g,2.291 mmol, 1 eq) in chloroform (10 mL) was added slowly at roomtemperature and stirred for 16 hours before being concentrated underreduced pressure. The solid material was resuspended in a mixture ofDCM, MeOH, EtOAc and 0.5 N NH₃ (MeOH), filtered through celite andconcentrated under reduced pressure. Purification by columnchromatography (ISCO, 12 g NH₂-silica column, 0-15% MeOH/DCM over a 15minute gradient) gave a mixture (1.75 g) of the desired product andstarting material which was carried forward without furtherpurification.

This mixture was dissolved in THF (72 mL) and DIPEA (1.25 mL, 7.16 mmol)and cooled to 0° C. Chloroacetyl chloride (0.63 mL, 7.88 mmol) was addedand the mixture was allowed to warm to room temperature. After 16 hours,the mixture was diluted with EtOAc and washed with saturated sodiumbicarbonate, water and brine. The resultant mixture was purified bycolumn chromatography (ISCO, dry load onto silica, 24 g column, 0-100%EtOAc/hexanes, over a 21 minute gradient) to give a white solid (0.56 g,1.745 mmol, 76% over 2 steps). ¹H NMR (400 MHz, Chloroform-d) δ 6.55 (s,1H), 4.48 (s, 1H), 4.05 (s, 2H), 3.30 (q, J=6.9 Hz, 2H), 3.10 (d, J=6.2Hz, 2H), 1.44 (s, 12H), 1.31 (s, 9H). ¹³C NMR (100 MHz, cdcl₃) δ 165.86,156.14, 77.36, 42.86, 40.73, 40.00, 30.18, 29.44, 29.26, 28.59, 26.86,26.82. LCMS 321.34 (M+H).

(2) Synthesis of dimethyl3-(2-((8-((tert-butoxycarbonyl)amino)octyl)amino)-2-oxoethoxy)phthalate

tert-butyl (8-(2-chloroacetamido)octyl)carbamate (0.468 g, 1.46 mmol, 1eq) was dissolved in MeCN (15 mL, 0.1 M). Dimethyl 3-hydroxyphthalate(0.337 g, 1.60 mmol, 1.1 eq) and cesium carbonate (1.308 g, 4.02 mmol,2.75 eq) were added. The flask was fitted with a reflux condenser andheated to 80° C. for 18 hours. The mixture was cooled to roomtemperature and diluted water and extracted once with chloroform andtwice with EtOAc. The combined organic layers were dried over sodiumsulfate, filtered and concentrated under reduced pressure.

The crude material was purified by column chromatography (ISCO, 24 gsilica column, 0-15% MeOH/DCM 20 minute gradient) to give a yellow oil(0.434 g, 0.878 mmol, 60%). ¹H NMR (400 MHz, Chloroform-d) δ 7.57 (dd,J=7.9, 0.8 Hz, 1H), 7.40 (t, J=8.1 Hz, 1H), 7.07 (dd, J=8.4, 0.7 Hz,1H), 6.89 (t, J=5.3 Hz, 1H), 4.63 (s, 1H), 4.52 (s, 2H), 3.88 (s, 3H),3.83 (s, 3H), 3.22 (q, J=6.9 Hz, 2H), 3.01 (q, J=6.4 Hz, 2H), 1.36 (s,12H), 1.20 (s, 9H). ¹³C NMR (100 MHz, cdcl₃) δ 167.89, 167.29, 165.54,155.97, 154.38, 130.95, 129.69, 124.96, 123.23, 116.86, 78.82, 68.05,52.83, 52.82, 52.66, 52.64, 40.54, 39.06, 29.97, 29.19, 29.10, 29.06,28.40, 26.66, 26.61. LCMS 495.42 (M+H).

(3) Synthesis of diaminooctyl-acetyl-O-thalidomide trifluoroacetate

Dimethyl3-(2-((8-((tert-butoxycarbonyl)amino)octyl)amino)-2-oxoethoxy)phthalate(0.434 g, 0.878 mmol, 1 eq) was dissolved in EtOH (8.8 mL, 0.1 M)Aqueous 3M NaOH (0.88 mL, 2.63 mmol, 3 eq) was added and the mixture washeated to 80° C. for 24 hours. The mixture was cooled to roomtemperature and diluted with 50 mL DCM and 10 mL 0.5 M HCl. The layerswere separated and the organic layer was washed with 20 mL water. Thecombined aqueous layers were then extracted 3 times with chloroform. Thecombined organic layers were dried over sodium sulfate, filtered andconcentrated under reduced pressure to give a yellow solid (0.329 g)that was carried forward without further purification. LCMS 467.41.

The resultant yellow solid (0.329 g) and 3-aminopiperidine-2,6-dionehydrochloride (0.121 g, 0.734 mmol, 1 eq) were dissolved in pyridine(7.3 mL, 0.1 M) and heated to 110° C. for 20 hours. The mixture wascooled to room temperature and concentrated under reduced pressure togive tert-butyl(8-(2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamido)octyl) carbamate as a black tar (0.293 g) which was carried forwardwithout purification (due to poor solubility). LCMS 559.45 (M+H).

The crude tert-butyl(8-(2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamido)octyl)carbamate(0.293 g) was dissolved in TFA (10 mL) and heated to 50° C. for 4 hours.The mixture was concentrated under reduced pressure, then concentratedunder reduced pressure. Purification by preparative HPLC gave a brownresidue (114.69 mg, 23% over 3 steps). ¹H NMR (400 MHz, Methanol-d₄) δ7.84-7.78 (m, 1H), 7.54 (d, J=7.3 Hz, 1H), 7.43 (d, J=8.5 Hz, 1H), 5.13(dd, J=12.5, 5.5 Hz, 1H), 4.76 (s, 2H), 3.32 (d, J=4.1 Hz, 1H), 3.30 (d,J=3.3 Hz, 1H), 2.94-2.84 (m, 3H), 2.80-2.70 (m, 2H), 2.19-2.12 (m, 1H),1.67-1.55 (m, 4H), 1.40-1.34 (m, 8H). ¹³C NMR (100 MHz, cd₃od) δ 174.57,171.37, 169.85, 168.26, 167.78, 156.26, 138.22, 134.91, 121.70, 119.28,117.97, 69.37, 50.57, 40.76, 40.08, 32.17, 30.19, 30.05, 30.01, 28.52,27.68, 27.33, 23.63. LCMS 459.41 (M+H).

Synthetic Example 72: Synthesis ofN-(3-(2-(2-(3-aminopropoxy)ethoxy)ethoxy)propyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate

(1) Synthesis of tert-butyl(1-chloro-2-oxo-7,10,13-trioxa-3-azahexadecan-16-yl)carbamate

tert-butyl (3-(2-(2-(3-aminopropoxy)ethoxy)ethoxy)propyl)carbamate (1.0g, 3.12 mmol, 1 eq) was dissolved in THF (31 mL, 0.1 M). DIPEA (0.543mL, 3.12 mmol, 1 eq) was added and the solution was cooled to 0° C.Chloroacetyl chloride (0.273 mL, 3.43 mmol, 1.1 eq) was added and themixture was warmed slowly to room temperature. After 24 hours, themixture was diluted with EtOAc and washed with saturated sodiumbicarbonate, water then brine. The organic layer was dried over sodiumsulfate, filtered and condensed to give a yellow oil (1.416 g) that wascarried forward without further purification. ¹H NMR (400 MHz,Chloroform-d) δ 7.24 (s, 1H), 5.00 (s, 1H), 3.98-3.89 (m, 2H), 3.54(dddt, J=17.0, 11.2, 5.9, 2.2 Hz, 10H), 3.47-3.40 (m, 2H), 3.37-3.31 (m,2H), 3.17-3.07 (m, 2H), 1.79-1.70 (m, 2H), 1.67 (p, J=6.1 Hz, 2H), 1.35(s, 9H). ¹³C NMR (100 MHz, cdcl₃) δ 165.83, 155.97, 78.75, 70.49, 70.47,70.38, 70.30, 70.14, 69.48, 42.61, 38.62, 38.44, 29.62, 28.59, 28.40.LCMS 397.37 (M+H).

(2) Synthesis of dimethyl3-((2,2-dimethyl-4,20-dioxo-3,9,12,15-tetraoxa-5,19-diazahenicosan-21-yl)oxy)phthalate

tert-butyl (1-chloro-2-oxo-7,10,13-trioxa-3-azahexadecan-16-yl)carbamate(1.41 g, 3.12 mmol, 1 eq) was dissolved in MeCN (32 mL, 0.1 M). Dimethyl3-hydroxyphthalate (0.721 g, 3.43 mmol, 1.1 eq) and cesium carbonate(2.80 g, 8.58 mmol, 2.75 eq) were added. The flask was fitted with areflux condenser and heated to 80° C. for 19 hours. The mixture wascooled to room temperature and diluted water and extracted once withchloroform and twice with EtOAc. The combined organic layers were driedover sodium sulfate, filtered and concentrated under reduced pressure.The crude material was purified by column chromatography (ISCO, 24 gsilica column, 0-15% MeOH/DCM 22 minute gradient) to give a yellow oil(1.5892 g, 2.78 mmol, 89% over two steps). ¹H NMR (400 MHz,Chloroform-d) δ 7.52 (d, J=7.8 Hz, 1H), 7.35 (t, J=8.1 Hz, 1H), 7.04 (d,J=8.3 Hz, 1H), 7.00 (t, J=5.3 Hz, 1H), 5.06 (s, 1H), 4.46 (s, 2H), 3.83(s, 3H), 3.78 (s, 3H), 3.47 (ddd, J=14.9, 5.5, 2.8 Hz, 8H), 3.39 (dt,J=9.4, 6.0 Hz, 4H), 3.29 (q, J=6.5 Hz, 2H), 3.09 (d, J=6.0 Hz, 2H), 1.70(p, J=6.5 Hz, 2H), 1.63 (p, J=6.3 Hz, 2H), 1.31 (s, 9H). ¹³C NMR (100MHz, cdcl₃) δ 167.68, 167.36, 165.45, 155.93, 154.41, 130.87, 129.60,125.01, 123.20, 117.06, 78.60, 70.40, 70.17, 70.06, 69.39, 68.67, 68.25,52.77, 52.57, 38.38, 36.58, 29.55, 29.20, 28.34. LCMS 571.47 (M+H).

(3) Synthesis ofN-(3-(2-(2-(3-aminopropoxy)ethoxy)ethoxy)propyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate

dimethyl3-((2,2-dimethyl-4,20-dioxo-3,9,12,15-tetraoxa-5,19-diazahenicosan-21-yl)oxy)phthalate(1.589 g, 2.78 mmol, 1 eq) was dissolved in EtOH (14 mL, 0.2 M). Aqueous3M NaOH (2.8 mL, 8.34 mmol, 3 eq) was added and the mixture was heatedto 80° C. for 22 hours. The mixture was then cooled to room temperature,diluted with 50 mL DCM and 20 mL 0.5 M HCl. The layers were separatedand the organic layer was washed with 25 mL water. The aqueous layerswere combined and extracted three times with 50 mL chloroform. Thecombined organic layers were dried over sodium sulfate, filtered andcondensed to give 1.53 g of material that was carried forward withoutfurther purification. LCMS 553.44.

The resultant material (1.53 g) and 3-aminopiperidine-2,6-dionehydrochloride (0.480 g, 2.92 mmol, 1 eq) were dissolved in pyridine(11.7 mL, 0.25 M) and heated to 110° C. for 17 hours. The mixture wascooled to room temperature and concentrated under reduced pressure togive crude tert-butyl(1-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)-2-oxo-7,10,13-trioxa-3-azahexadecan-16-yl)carbamateas a black sludge (3.1491 g) that was carried forward without furtherpurification. LCMS 635.47.

The crude tert-butyl(1-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)-2-oxo-7,10,13-trioxa-3-azahexadecan-16-yl)carbamate(3.15 g) was dissolved in TFA (20 mL) and heated to 50° C. for 2.5hours. The mixture was cooled to room temperature, diluted with MeOH andconcentrated under reduced pressure. The material was purified bypreparative HPLC to giveN-(3-(2-(2-(3-aminopropoxy)ethoxy)ethoxy)propyl)-2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamidetrifluoroacetate (1.2438 g, 1.9598 mmol, 71% over 3 steps) as a dark redoil. ¹H NMR (400 MHz, Methanol-d₄) δ 7.77 (dd, J=8.3, 7.5 Hz, 1H), 7.49(d, J=7.3 Hz, 1H), 7.40 (d, J=8.5 Hz, 1H), 5.12 (dd, J=12.8, 5.5 Hz,1H), 4.75 (s, 2H), 3.68-3.51 (m, 12H), 3.40 (t, J=6.8 Hz, 2H), 3.10 (t,J=6.4 Hz, 2H), 2.94-2.68 (m, 3H), 2.16 (dtd, J=12.6, 5.4, 2.5 Hz, 1H),1.92 (p, J=6.1 Hz, 2H), 1.86-1.77 (m, 2H). ¹³C NMR (100 MHz, cd₃od) δ173.17, 169.97, 168.48, 166.87, 166.30, 154.82, 136.89, 133.41, 120.29,117.67, 116.58, 69.96, 69.68, 69.60, 68.87, 68.12, 67.92, 49.19, 38.62,36.14, 30.80, 28.92, 26.63, 22.22. LCMS 536.41 (M+H).

Synthetic Example 73: Synthesis ofN-(6-aminohexyl)-2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindoline-5-carboxamide

(1) Synthesis of2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindoline-5-carboxylic acid

1,3-dioxo-1,3-dihydroisobenzofuran-5-carboxylic acid (0.192 g, 1 mmol, 1eq) and 3-aminopiperidine-2,6-dione hydrochloride (0.165 g, 1 mmol, 1eq) were dissolved in DMF (2.5 mL) and acetic acid (5 mL) and heated to80° C. for 24 hours. The mixture was then concentrated under reducedpressure and diluted with EtOH, from which a precipitate slowly formed.The precipitate was washed twice with EtOH to give a white solid (84.8mg, 0.28 mmol, 28%). ¹H NMR (400 MHz, DMSO-d₆) 13.74 (s, 1H), 11.12 (s,1H), 8.39 (dd, J=7.8, 1.4 Hz, 1H), 8.26 (s, 1H), 8.04 (d, J=7.8 Hz, 1H),5.18 (dd, J=12.8, 5.4 Hz, 1H), 2.93-2.88 (m, 1H), 2.84 (d, J=4.7 Hz,0H), 2.66-2.50 (m, 2H), 2.12-1.99 (m, 1H). LCMS 303.19 (M+H).

(2) Synthesis of tert-butyl(6-(2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindoline-5-carboxamido)hexyl)carbamate

2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindoline-5-carboxylic acid (22.7mg, 0.0751 mmol, 1 eq) and HATU (31.4 mg, 0.0826 mmol, 1.1 eq) weredissolved in DMF (0.75 mL). After 5 minutes, DIPA (39.2 microliters,0.225 mmol, 3 eq) was added. After an additional 5 minutes, tert-butyl(6-aminohexyl)carbamate (19.5 mg, 0.0901 mmol, 1.2 eq) was added as asolution in DMF (0.75 mL). The mixture was stirred for 20 hours, thendiluted with EtOAc. The organic layer was washed three times with brine,dried over sodium sulfate and concentrated under reduced pressure.Purification by column chromatography (ISCO, 4 g column, 0-10% MeOH/DCM,25 minute gradient) to give a yellow oil (17.18 mg, 0.03432 mmol, 46%).¹H NMR (400 MHz, Chloroform-d) δ 8.29 (d, J=6.2 Hz, 2H), 8.16 (s, 1H),7.94 (d, J=8.4 Hz, 1H), 6.91 (s, 1H), 5.00 (dd, J=12.4, 5.3 Hz, 1H),4.58 (s, 1H), 3.47 (q, J=6.7 Hz, 2H), 3.14 (q, J=8.5, 7.3 Hz, 2H),2.97-2.69 (m, 3H), 2.17 (ddd, J=10.4, 4.8, 2.6 Hz, 1H), 1.65 (p, J=6.9Hz, 2H), 1.53-1.32 (m, 15H). ¹³C NMR (100 MHz, cdcl₃) δ 174.69, 170.77,167.86, 166.67, 165.27, 156.49, 141.06, 133.95, 133.71, 132.13, 124.21,122.27, 77.36, 49.71, 39.75, 31.54, 30.27, 29.22, 28.57, 25.70, 25.37,22.73. LCMS 501.28 (M+H).

(3) Synthesis ofN-(6-aminohexyl)-2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindoline-5-carboxamide

tert-butyl(6-(2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindoline-5-carboxamido)hexyl)carbamate(17.18 mg, 0.343 mmol, 1 eq) was dissolved in TFA (1 mL) and heated to50° C. for 2 hours. The mixture was concentrated under reduced pressureto give a yellow oil (13.29 mg) which was deemed sufficiently purewithout further purification. ¹H NMR (400 MHz, Methanol-d₄) δ 8.27 (dd,J=9.3, 1.3 Hz, 2H), 7.99 (d, J=7.6 Hz, 1H), 5.18 (dd, J=12.5, 5.4 Hz,1H), 3.48-3.40 (m, 2H), 2.96-2.84 (m, 3H), 2.76 (ddd, J=17.7, 8.1, 3.7Hz, 2H), 2.20-2.12 (m, 1H), 1.75-1.63 (m, 4H), 1.53-1.43 (m, 4H). LCMS401.31 (M+H).

Synthetic Example 74: Synthesis of2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetic acid

(1) Synthesis of2-(2,6-dioxopiperidin-3-yl)-4-hydroxyisoindoline-1,3-dione

4-hydroxyisobenzofuran-1,3-dione (0.773 g, 4.71 mmol, 1 eq) and3-aminopiperidine-2,6-dione hydrochloride (0.775 g, 4.71 mmol, 1 eq)were dissolved in pyridine (19 mL) and heated to 110° C. for 16 hours.The mixture was concentrated under reduced pressure and purified bycolumn chromatography (ISCO, 12 g silica column, 0-10% MeOH/DCM, 25minute gradient) to give an off white solid (1.14 g, 4.16 mmol, 88%). ¹HNMR (400 MHz, DMSO-d₆) δ 11.19 (s, 1H), 11.07 (s, 1H), 7.65 (dd, J=8.3,7.3 Hz, 1H), 7.31 (d, J=7.2 Hz, 1H), 7.24 (d, J=8.4 Hz, 1H), 5.07 (dd,J=12.8, 5.4 Hz, 1H), 2.88 (ddd, J=17.7, 14.2, 5.4 Hz, 1H), 2.63-2.50 (m,2H), 2.11-1.95 (m, 1H). LCMS 275.11 (M+H).

(2) Synthesis of tert-butyl2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetate

2-(2,6-dioxopiperidin-3-yl)-4-hydroxyisoindoline-1,3-dione (218.8 mg,0.798 mmol, 1 eq) was dissolved in DMF (8 mL). Potassium carbonate(165.9 mg, 1.20 mmol, 1.5 eq) was added, followed by tert-butylbromoacetate (118 microliters, 0.798 mmol, 1 eq) and the mixture wasstirred at room temperature for 3 hours. The mixture was diluted withEtOAc and washed once with water and twice with brine. Purification bycolumn chromatography (ISCO, 12 g silica column, 0-100% EtOAc/hex, 17minute gradient) gave a white solid (0.26 g, 0.669 mmol, 84%). ¹H NMR(400 MHz, Chloroform-d) δ 8.74 (s, 1H), 7.61 (dd, J=8.4, 7.3 Hz, 1H),7.46-7.41 (m, 1H), 7.06 (d, J=8.3 Hz, 1H), 4.98-4.92 (m, 1H), 4.74 (s,2H), 2.83-2.69 (m, 3H), 2.12-2.04 (m, 1H), 1.43 (s, 9H). ¹³C NMR (100MHz, cdcl₃) a 171.58, 168.37, 166.96, 166.87, 165.49, 155.45, 136.27,133.89, 119.78, 117.55, 116.83, 83.05, 66.52, 49.20, 31.37, 28.03,22.55. LCMS 411.23 (M+Na).

(3) Synthesis of2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetic acid

tert-butyl2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetate(47.5 mg, 0.122 mmol, 1 eq) was dissolved in TFA (1.3 mL) at roomtemperature. After 3 hours, the mixture was diluted with DCM andconcentrated under reduced pressure to yield a white solid (42.27 mg),which was deemed sufficiently pure without further purification. ¹H NMR(400 MHz, Methanol-d₄) δ 7.76 (dd, J=8.5, 7.3 Hz, 1H), 7.50 (d, J=7.3Hz, 1H), 7.34 (d, J=8.5 Hz, 1H), 5.11 (dd, J=12.5, 5.5 Hz, 1H), 4.96 (s,2H), 2.87 (ddd, J=17.8, 14.2, 5.0 Hz, 1H), 2.80-2.65 (m, 2H), 2.18-2.09(m, 1H). LCMS 333.15 (M+H).

Heterobifunctional Compound Pharmaceutical Compositions

In another aspect of the present application, pharmaceuticalcompositions are provided, which comprise any one of theheterobifunctional compounds described herein (or a prodrug,pharmaceutically acceptable salt or other pharmaceutically acceptablederivative thereof), and optionally comprise a pharmaceuticallyacceptable carrier. According to the present application, apharmaceutically acceptable derivative includes, but is not limited to,pharmaceutically acceptable salts, esters, salts of such esters, or apro-drug or other adduct or derivative of a compound of this applicationwhich upon administration to a patient in need is capable of providing,directly or indirectly, a heterobifunctional compound as otherwisedescribed herein, or a metabolite or residue thereof.

As used herein, the term “pharmaceutically acceptable salt” refers tothose salts which are, within the scope of sound medical judgment,suitable for use in contact with the tissues of humans and lower animalswithout undue toxicity, irritation, allergic response and the like, andare commensurate with a reasonable benefit/risk ratio. Pharmaceuticallyacceptable salts of amines, carboxylic acids, and other types ofcompounds, are well known in the art. For example, S. M. Berge, et al.describe pharmaceutically acceptable salts in detail in J PharmaceuticalSciences 66 (1977): 1-19, incorporated herein by reference. The saltscan be prepared in situ during the final isolation and purification ofthe heterobifunctional compounds of the application, or separately byreacting a free base or free acid function with a suitable reagent, asdescribed generally below. For example, a free base function can bereacted with a suitable acid. Furthermore, where the heterobifunctionalcompounds of the application carry an acidic moiety, suitablepharmaceutically acceptable salts thereof may, include metal salts suchas alkali metal salts, e.g. sodium or potassium salts; and alkalineearth metal salts, e.g. calcium or magnesium salts. Examples ofpharmaceutically acceptable, nontoxic acid addition salts are salts ofan amino group formed with inorganic acids such as hydrochloric acid,hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid orwith organic acids such as acetic acid, oxalic acid, maleic acid,tartaric acid, citric acid, succinic acid or malonic acid or by usingother methods used in the art such as ion exchange. Otherpharmaceutically acceptable salts include adipate, alginate, ascorbate,aspartate, benzenesulfonate, benzoate, bisulfate, borate, butyrate,camphorate, camphorsulfonate, citrate, cyclopentanepropionate,digluconate, dodecylsulfate, ethanesulfonate, formate, fumarate,glucoheptonate, glycerophosphate, gluconate, hemisulfate, heptanoate,hexanoate, hydroiodide, 2-hydroxy-ethanesulfonate, lactobionate,lactate, laurate, lauryl sulfate, malate, maleate, malonate,methanesulfonate, 2-naphthalenesulfonate, nicotinate, nitrate, oleate,oxalate, palmitate, pamoate, pectinate, persulfate, 3-phenylpropionate,phosphate, picrate, pivalate, propionate, stearate, succinate, sulfate,tartrate, thiocyanate, p-toluenesulfonate, undecanoate, valerate salts,and the like. Representative alkali or alkaline earth metal saltsinclude sodium, lithium, potassium, calcium, magnesium, and the like.Further pharmaceutically acceptable salts include, when appropriate,nontoxic ammonium, quaternary ammonium, and amine cations formed usingcounterions such as halide, hydroxide, carboxylate, sulfate, phosphate,nitrate, lower alkyl sulfonate and aryl sulfonate.

Additionally, as used herein, the term “pharmaceutically acceptableester” refers to esters that hydrolyze in vivo and include those thatbreak down readily in the human body to leave the parentheterobifunctional compound or a salt thereof. Suitable ester groupsinclude, for example, those derived from pharmaceutically acceptablealiphatic carboxylic acids, particularly alkanoic, alkenoic,cycloalkanoic and alkanedioic acids, in which each alkyl or alkenylmoiety advantageously has not more than 6 carbon atoms. Examples ofparticular esters include formates, acetates, propionates, butyrates,acrylates and ethylsuccinates.

Furthermore, the term “pharmaceutically acceptable prodrugs” as usedherein refers to those prodrugs of the heterobifunctional compounds ofthe present application which are, within the scope of sound medicaljudgment, suitable for use in contact with the issues of humans andlower animals with undue toxicity, irritation, allergic response, andthe like, commensurate with a reasonable benefit/risk ratio, andeffective for their intended use, as well as the zwitterionic forms,where possible, of the compounds of the application. The term “prodrug”refers to compounds that are rapidly transformed in vivo to yield theparent compound of the above formula, for example by hydrolysis inblood. A thorough discussion is provided in T. Higuchi and V. Stella,Pro-drugs as Novel Delivery Systems, Vol. 14 of the A.C.S. SymposiumSeries, and in Edward B. Roche, ed., Bioreversible Carriers in DrugDesign, American Pharmaceutical Association and Pergamon Press, (1987),both of which are incorporated herein by reference.

As described above, the pharmaceutical heterobifunctional compoundcompositions of the present application additionally comprise apharmaceutically acceptable carrier, which, as used herein, includes anyand all solvents, diluents, or other liquid vehicle, dispersion orsuspension aids, surface active agents, isotonic agents, thickening oremulsifying agents, preservatives, solid binders, lubricants and thelike, as suited to the particular dosage form desired. Remington'sPharmaceutical Sciences, Sixteenth Edition, E. W. Martin (MackPublishing Co., Easton, Pa., (1980)) discloses various carriers used informulating pharmaceutical compositions and known techniques for thepreparation thereof. Except insofar as any conventional carrier mediumis incompatible with the compounds of the application, such as byproducing any undesirable biological effect or otherwise interacting ina deleterious manner with any other component(s) of the pharmaceuticalcomposition, its use is contemplated to be within the scope of thisapplication. Some examples of materials which can serve aspharmaceutically acceptable carriers include, but are not limited to,sugars such as lactose, glucose and sucrose; starches such as cornstarch and potato starch; cellulose and its derivatives such as sodiumcarboxymethyl cellulose, ethyl cellulose and cellulose acetate; powderedtragacanth; malt; gelatine; talc; excipients such as cocoa butter andsuppository waxes; oils such as peanut oil, cottonseed oil; saffloweroil, sesame oil; olive oil; corn oil and soybean oil; glycols; such aspropylene glycol; esters such as ethyl oleate and ethyl laurate; agar;buffering agents such as magnesium hydroxide and aluminum hydroxide;alginic acid; pyrogen free water; isotonic saline; Ringer's solution;ethyl alcohol, and phosphate buffer solutions, as well as othernon-toxic compatible lubricants such as sodium lauryl sulfate andmagnesium stearate, as well as coloring agents, releasing agents,coating agents, sweetening, flavoring and perfuming agents,preservatives and antioxidants can also be present in the composition,according to the judgment of the formulator.

Liquid dosage forms for oral administration include, but are not limitedto, pharmaceutically acceptable emulsions, microemulsions, solutions,suspensions, syrups and elixirs. In addition to the active compounds,the liquid dosage forms may contain inert diluents commonly used in theart such as, for example, water or other solvents, solubilizing agentsand emulsifiers such as ethyl alcohol, isopropyl alcohol, ethylcarbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propyleneglycol, 1,3-butylene glycol, dimethylformamide, oils (in particular,cottonseed, groundnut, corn, germ, olive, castor, and sesame oils),glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fattyacid esters of sorbitan, and mixtures thereof. Besides inert diluents,the oral compositions can also include adjuvants such as wetting agents,emulsifying and suspending agents, sweetening, flavoring, and perfumingagents.

Injectable preparations, for example, sterile injectable aqueous oroleaginous suspensions may be formulated according to the known artusing suitable dispersing or wetting agents and suspending agents. Thesterile injectable preparation may also be a sterile injectablesolution, suspension or emulsion in a nontoxic parenterally acceptablediluent or solvent, for example, as a solution in 1,3-butanediol. Amongthe acceptable vehicles and solvents that may be employed are water,Ringer's solution, U.S.P. and isotonic sodium chloride solution. Inaddition, sterile, fixed oils are conventionally employed as a solventor suspending medium. For this purpose, any bland fixed oil can beemployed including synthetic mono- or diglycerides. In addition, fattyacids such as oleic acid are used in the preparation of injectables.

The injectable formulations can be sterilized, for example, byfiltration through a bacterial-retaining filter, or by incorporatingsterilizing agents in the form of sterile solid compositions which canbe dissolved or dispersed in sterile water or other sterile injectablemedium prior to use.

In order to prolong the effect of a drug, it is often desirable to slowthe absorption of the drug from subcutaneous or intramuscular injection.This may be accomplished by the use of a liquid suspension orcrystalline or amorphous material with poor water solubility. The rateof absorption of the drug then depends upon its rate of dissolutionthat, in turn, may depend upon crystal size and crystalline form.Alternatively, delayed absorption of a parenterally administered drugform is accomplished by dissolving or suspending the drug in an oilvehicle. Injectable depot forms are made by forming microencapsulematrices of the drug in biodegradable polymers such aspolylactide-polyglycolide. Depending upon the ratio of drug to polymerand the nature of the particular polymer employed, the rate of drugrelease can be controlled. Examples of other biodegradable polymersinclude poly(orthoesters) and poly(anhydrides). Depot injectableformulations are also prepared by entrapping the drug in liposomes ormicroemulsions which are compatible with body tissues.

Compositions for rectal or vaginal administration are preferablysuppositories which can be prepared by mixing the compounds of thisapplication with suitable non-irritating excipients or carriers such ascocoa butter, polyethylene glycol or a suppository wax which are solidat ambient temperature but liquid at body temperature and therefore meltin the rectum or vaginal cavity and release the active compound.

Solid dosage forms for oral administration include capsules, tablets,pills, powders, and granules. In such solid dosage forms, the activecompound is mixed with at least one inert, pharmaceutically acceptableexcipient or carrier such as sodium citrate or dicalcium phosphateand/or a) fillers or extenders such as starches, lactose, sucrose,glucose, mannitol, and silicic acid, b) binders such as, for example,carboxymethylcellulose, alginates, gelatin, polyvinylpyrrolidinone,sucrose, and acacia, c) humectants such as glycerol, d) disintegratingagents such as agar-agar, calcium carbonate, potato or tapioca starch,alginic acid, certain silicates, and sodium carbonate, e) solutionretarding agents such as paraffin, f) absorption accelerators such asquaternary ammonium compounds, g) wetting agents such as, for example,cetyl alcohol and glycerol monostearate, h) absorbents such as kaolinand bentonite clay, and i) lubricants such as talc, calcium stearate,magnesium stearate, solid polyethylene glycols, sodium lauryl sulfate,and mixtures thereof. In the case of capsules, tablets and pills, thedosage form may also comprise buffering agents.

Solid compositions of a similar type may also be employed as fillers insoft and hard-filled gelatin capsules using such excipients as lactoseor milk sugar as well as high molecular weight polyethylene glycols andthe like. The solid dosage forms of tablets, dragees, capsules, pills,and granules can be prepared with coatings and shells such as entericcoatings and other coatings well known in the pharmaceutical formulatingart. They may optionally contain opacifying agents and can also be of acomposition that they release the active ingredient(s) only, orpreferentially, in a certain part of the intestinal tract, optionally,in a delayed manner.

Examples of embedding compositions that can be used include polymericsubstances and waxes. Solid compositions of a similar type may also beemployed as fillers in soft and hard-filled gelatin capsules using suchexcipients as lactose or milk sugar as well as high molecular weightpolyethylene glycols and the like.

The active heterobifunctional compounds can also be inmicro-encapsulated form with one or more excipients as noted above. Thesolid dosage forms of tablets, dragees, capsules, pills, and granulescan be prepared with coatings and shells such as enteric coatings,release controlling coatings and other coatings well known in thepharmaceutical formulating art. In such solid dosage forms the activeheterobifunctional compound may be admixed with at least one inertdiluent such as sucrose, lactose and starch. Such dosage forms may alsocomprise, as in normal practice, additional substances other than inertdiluents, e.g., tableting lubricants and other tableting aids such asmagnesium stearate and microcrystalline cellulose. In the case ofcapsules, tablets and pills, the dosage forms may also comprisebuffering agents. They may optionally contain opacifying agents and canalso be of a composition that they release the active ingredient(s)only, or preferentially, in a certain part of the intestinal tract,optionally, in a delayed manner. Examples of embedding compositionswhich can be used include polymeric substances and waxes.

The present application encompasses pharmaceutically acceptable topicalformulations of inventive compounds. The term “pharmaceuticallyacceptable topical formulation”, as used herein, means any formulationwhich is pharmaceutically acceptable for intradermal administration of acompound of the application by application of the formulation to theepidermis. In certain embodiments of the application, the topicalformulation comprises a carrier system. Pharmaceutically effectivecarriers include, but are not limited to, solvents (e.g., alcohols, polyalcohols, water), creams, lotions, ointments, oils, plasters, liposomes,powders, emulsions, microemulsions, and buffered solutions (e.g.,hypotonic or buffered saline) or any other carrier known in the art fortopically administering pharmaceuticals. A more complete listing ofart-known carriers is provided by reference texts that are standard inthe art, for example, Remington's Pharmaceutical Sciences, 16th Edition,(1980) and 17th Edition, (1985), both published by Mack PublishingCompany, Easton, Pa., the disclosures of which are incorporated hereinby reference in their entireties. In certain other embodiments, thetopical formulations of the application may comprise excipients. Anypharmaceutically acceptable excipient known in the art may be used toprepare the inventive pharmaceutically acceptable topical formulations.Examples of excipients that can be included in the topical formulationsof the application include, but are not limited to, preservatives,antioxidants, moisturizers, emollients, buffering agents, solubilizingagents, other penetration agents, skin protectants, surfactants, andpropellants, and/or additional therapeutic agents used in combination tothe inventive compound. Suitable preservatives include, but are notlimited to, alcohols, quaternary amines, organic acids, parabens, andphenols. Suitable antioxidants include, but are not limited to, ascorbicacid and its esters, sodium bisulfite, butylated hydroxytoluene,butylated hydroxyanisole, tocopherols, and chelating agents like EDTAand citric acid. Suitable moisturizers include, but are not limited to,glycerine, sorbitol, polyethylene glycols, urea, and propylene glycol.Suitable buffering agents for use with the application include, but arenot limited to, citric, hydrochloric, and lactic acid buffers. Suitablesolubilizing agents include, but are not limited to, quaternary ammoniumchlorides, cyclodextrins, benzyl benzoate, lecithin, and polysorbates.Suitable skin protectants that can be used in the topical formulationsof the application include, but are not limited to, vitamin E oil,allatoin, dimethicone, glycerin, petrolatum, and zinc oxide.

In certain embodiments, the pharmaceutically acceptable topicalformulations of the application comprise at least a compound of theapplication and a penetration enhancing agent. The choice of topicalformulation will depend or several factors, including the condition tobe treated, the physicochemical characteristics of the inventivecompound and other excipients present, their stability in theformulation, available manufacturing equipment, and costs constraints.As used herein the term “penetration enhancing agent” means an agentcapable of transporting a pharmacologically active compound through thestratum corneum and into the epidermis or dermis, preferably, withlittle or no systemic absorption. A wide variety of compounds have beenevaluated as to their effectiveness in enhancing the rate of penetrationof drugs through the skin. See, for example, Maibach H. I. and Smith H.E. (eds.), Percutaneous Penetration Enhancers, CRC Press, Inc., BocaRaton, Fla. (1995), which surveys the use and testing of various skinpenetration enhancers, and Buyuktimkin et al., Chemical Means ofTransdermal Drug Permeation Enhancement in Transdermal and Topical DrugDelivery Systems, Gosh T. K., Pfister W. R., Yum S. I. (eds.),Interpharm Press Inc., Buffalo Grove, Ill. (1997). In certain exemplaryembodiments, penetration agents for use with the application include,but are not limited to, triglycerides (e.g., soybean oil), aloecompositions (e.g., aloe-vera gel), ethyl alcohol, isopropyl alcohol,octolyphenylpolyethylene glycol, oleic acid, polyethylene glycol 400,propylene glycol, N-decylmethylsulfoxide, fatty acid esters (e.g.,isopropyl myristate, methyl laurate, glycerol monooleate, and propyleneglycol monooleate), and N-methylpyrrolidone.

In certain embodiments, the compositions may be in the form ofointments, pastes, creams, lotions, gels, powders, solutions, sprays,inhalants or patches. In certain exemplary embodiments, formulations ofthe compositions according to the application are creams, which mayfurther contain saturated or unsaturated fatty acids such as stearicacid, palmitic acid, oleic acid, palmito-oleic acid, cetyl or oleylalcohols, and stearic acid are useful. Creams of the application mayalso contain a non-ionic surfactant, for example, polyoxy-40-stearate.In certain embodiments, the active component is admixed under sterileconditions with a pharmaceutically acceptable carrier and any neededpreservatives or buffers as may be required. Ophthalmic formulation,eardrops, and eye drops are also contemplated as being within the scopeof this application. Additionally, the present application contemplatesthe use of transdermal patches, which have the added advantage ofproviding controlled delivery of a compound to the body. Such dosageforms are made by dissolving or dispensing the compound in the propermedium. As discussed above, penetration enhancing agents can also beused to increase the flux of the compound across the skin. The rate canbe controlled by either providing a rate controlling membrane or bydispersing the compound in a polymer matrix or gel.

It will also be appreciated that certain heterobifunctional compounds ofpresent application can exist in free form for treatment, or whereappropriate, as a pharmaceutically acceptable derivative thereof.According to the present application, a pharmaceutically acceptablederivative includes, but is not limited to, pharmaceutically acceptablesalts, esters, salts of such esters, or a prodrug or other adduct orderivative of a compound of this application which upon administrationto a patient in need is capable of providing, directly or indirectly, acompound as otherwise described herein, or a metabolite or residuethereof.

In one embodiment the heterobifunctional compound as any one of thepharmaceutical compositions described above, is administered to a hostin need thereof to stop expression of a protein of interest by action ona synthetic endogenous protein-dTAG hybrid protein. Alternatively, theheterobifunctional compound as any one of the pharmaceuticalcompositions described above, is administered to a host in need thereofto start expression of a protein of interest by action on a syntheticendogenous protein-dTAG hybrid protein.

EXAMPLES

Examples are further provided of exemplary engineering of endogenousprotein-dTAG hybrid proteins having a dTAG capable of being bound by orbinding to a heterobifunctional compound, which, when exposed to theheterobifunctional compound is degraded by the ubiquitin proteasomalpathway (UPP). The examples are exemplary only and are not intended tobe limited, instead serving as illustrations of a method of modulatingthe expression of a protein-of-interest through specific degradation ofthe target with a heterobifunctional compound targeting the endogenousprotein-dTAG hybrid protein.

Example 1: Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9)-dTAG

To further describe the targeting of endogenous proteins of interest fordegradation through the use of a dTAG as contemplated herein, thetargeting of an exemplary protein of interest, the gene product ofPCSK9, for insertion of a nucleic acid encoding a dTAG is illustrated.

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is an enzyme thatcontrols cholesterol homeostasis. PCSK9 regulates the expression of lowdensity lipoprotein (LDL) receptor in the liver. LDLR binds to, andinternalizes free LDL cholesterol from the blood, effectively reducingcholesterol levels. When PCSK9 is deregulated, the enzyme binds anddegrades LDLR, thus increasing free blood cholesterol resulting inhypercholesterolemia. Inhibition, or degradation of PCSK9 would restoreLDLC expression and effectively reduce free blood cholesterol in theliver. Since increased levels of free LDL are associated with anincreased risk of cardiac disease, efforts to reduce PCKS9 expression oractivity are of great interest to the community.

To engineer the endogenous protein-dTAG hybrid protein, a homologousdonor construct is cloned that includes a left homology region (portionof intron 1), dTAG nucleic acid sequence (derived from the dTAGFKBP*—SEQ. ID. NO.: 2) cloned in frame with exon 1 of PCSK9, and a righthomology region (portion of intron 2). The dTAG peptide is cloned inframe with a 2× glycine linker. To initiate homologous recombination, aCRISPR sgRNA is designed to target the coding sequence PCSK9 in exon 1.CAS9 expression induces a double strand break which is repaired byhomologous recombination repair using the donor construct as template.The end result is a gene locus with dTAG nucleic acid cloned in framewith exon 1 of PCSK9.

As derived, the resultant nucleic acid sequence including the in framedTAG nucleic acid insert results in the following genomic nucleic acidsequence, wherein lower case letters indicate intronic sequences of thePCSK9 genomic sequence, capital, underlined sequences indicate the sgRNAtarget

(SEQ. ID. NO.: 45) (GAGGGAGATTTGACACACACAGG),ATG indicates the transcriptional start site of the PCSK9 protein orPCSK9-dTAG hybrid, capital letters indicate the exon coding sequence ofthe PCSK9 protein, and capital, italicized letters indicate the in frameinsertion of the FKBP* derived dTAG nucleic acid with a 2X glycinelinker (GGGGGG) (SEQ. ID. NO.: 46). An illustration representing theexemplified HR strategy is provided for in FIG. 2.

Targeted PCSK9 Genomic Locus (SEQ. ID. NO.: 47)gtgtggggctgcctccccgagcttccatctgccgctggggccacaccccaggcccagggatgggaccccacagtggtcacatcatcttgcagcagaacccaggtacagctcctggagcagatggtggtcccaagcacgggtgggaccagaaaggactctcacctgggctaactcagctgcagcctcagttccctcctcacacacgacgaggaacatggactggaagcctgcccagcaggccttctgctcgatgtgcgttgtgtggcttacgtccagggagggaagcagcctctgtgctgtcttctagataagcctgtattccccgggctgtctgccaatgtatccagttgtcccgtcagcctggaagactgagggaaaaccttgggctgcttcctgagcacctgtatcccctgcagccagcccggggcctctgctaggagcagactgagcatggcttatgggcctggcaccatctggcctctgcccaccttgctggccttgtcttgtgtctgccccttcgacattccatagcccagctcaatatctagtggttcctctagggtggcgagcactgtttggtctccagatgtcttcaggtcggagctcacagcgctctcagccaccccttcccagtgtagcaccgggcacatggtagatgcctattgatgagtgaaagctcctaacacactcagagagcaaggactccgcctcatcccacagcctgggaggagaggcagactgccaaggacctgctcagcatgctacagaagaaaccaaagtgcccacgggactgatcagtggagcttcctgccgagactggaggccttagggcagggtagacagtgtgtgtgcaggctggggactcacagttcggactgtgcccagacctactagcatagtgggtgggtgggaggatgcgggactgggggccgaccttgcctgaaattcatgtgggatctcagagcagccactgaattgctctgtagggggctaaatagtggcccccacagatacacacacccagacagagcctgtgagccagaccttatttggagaaaaggtctttgtagatgtaattaagcatctcaagatggcatcatctggattatgcggtgggctgtaagtcctgtgatgtgtcttt ATGAGAGAAAGGCAGAGGGAGATTTGACACACACAGGAGGGGCCACGTGGAGACAGAGGTGGAGATTGGAGAAATGTGGCCACAAGCCAGGGAACACCAGCAGCCACCAGAAGCCGGAAGACGTGAGGCAGGGTTCTTCCCAGAGCCTTCGCTGCTGAGTCTGGGAATTTGTGACCGAAGCCATAAGAAGTGGGTACACGCCCTGAGCCTCCCACACTTGCTCACCTGTCCTGAGATGAGAATCTCTACTCTGCAGCATATTTGGAGGATCACTGCGGGGGCCACAGAGGTGCTGTTCAGATGGCACTTCAGAAGACTCAGGAGACCCTGGGGCAGGAGCAGTTTGACTGACAGCCCAGAGGGCTGCCCTCTGATTCCACCTGAGGCCCTGCTTTTCCTGGCTGCAGGGGTTCCAGGGCCAGGCCATTTCCGCTGGCGCAGGACTCTGCTAGCAGCAACCTGCCTGAAGTCTTCCTTTGGCCTGGCTGAGAGTTTCTGAGACCTGCGCTGGAGCGGAGGTGCTTCCTTCCTTGCTTCCTTTCTTCCTCTCTCCCTTCTCCATCCAGCAGGCTGGACCTGCCTGGCATCTGTGAGCTCTCCCTACTTTCTCCTATACCCTAACCTTTGTCCTGCATGGGCGACTCCCCCAGTGAGTCTCTTGCAGCTTTTACCCCAGTGCCTGCTTCTTGGAGAATCCAAACTGATCCAGTTAGGGATGATAAAGTGTAGGGTAGGCGCTCGGTGACTGTTTTCTCTGAGGTTGTGACTCGTGTGAGGCAGAAGCAGTCCCCGTGAGCCCTCCTGGTATCTTGTGGAGTGGAGAACGCTTGGACCTGGAGCCAGGAGGCCCAGACATACATCCTGTCCGAGCTGCAGCTTCCTGTCTCTAAAATGAGCCGGCCAGCGCAGGTGGCCAGACATCACTGTTATTCTCCTTTGAGTCTTTAAATCTTGTTGTCTTTCTTGCAGACTCGGTGAGCTGTGAAAGGCTATAATAGGGGCTTTATTTTACACTTTGATACTATTTTTTGAACATTCATATTATTGTTAGATATTGATATTCATATGAAGGAGCAGGATGACTTGGGTCCTTCTTGGCAGTAGCATTGCCAGCTGATGGCCTTGGACAGTTACCTGCCCTCTCTAGGCCTCCCTTTCCTTGTCTATGAAATACATTATAGAATAGGATGTAGTGTGTGAGGATTTTTTGGAGGTTAAACGAGTGAATATATTTAAGGCGCTTTCACCAGTGCCTGGGATGTGCTCTGTAGTTTCTGTGTGTTAACTATAAGGTTGACTTTATGCTCATTCCCTCCTCTCCCACAAATGtcgccttggaaagacggaggcagcctggtggaggtgtatctcctagacaccagcatacagagtgaccaccgggaaatcgagggcagggtcatggtcaccgacttcgagaatgtgcccgaggaggacgggacccgcttccacagacaggtaagcacggccgtctgatgggagggctgcctctgcccatatccccatcctggaggtgggtggggactgccaccccagagcgttgcagctgtactcctgggttgcaccccccccagctgtcactgtcccctccctgccatcagttgtgggaagggcgttcatccatccagccacctgctgatttgttatagggtggagggggggtctttctcatgtggtccttgtgttcgtcgagcaggccagcaagtgtgacagtcatggcacccacctggcaggggtggtcagcggccgggatgccggcgtggccaagggtgccagcatgcgcagcctgcgcgtgctcaactgccaagggaagggcacggttagcggcaccctcataggtaagtgatggccccagacgctggtctctctccatctggacctggcctgggaggtggcttgggctgggcccagggagagctaatgtctcctaaccaagaatgctgtggcagcctctgccgcagagccagagaaccagagtgccaaggctggcagggttcccagtggccacgagtgcagatgaagaaacccaggccccaagagggtcatgcaggtagcccagggagttcagccttgaccctgggtcaatgacctttccacagttccacactgctccccttttaaaatccggtgatgtctttatgtcttttgttatgttatcttcaatgtggagggactcgaggtgatctaagcaaactttttctatcttctgcttgcatacctctgagaccaggggactcactcacttgcatgactgggccctgcaggtcacactggccaggcagatgtggtggaggaactggcagaggactttttctagactgtgactacatttagtccacccagcggcccccctatgaagtccagttgagaactaggactctgggggccggtggacagagaagag.Resultant PCSK9-dTAG Hybrid (SEQ. ID. NO.: 48)gtgtggggctgcctccccgagcttccatctgccgctggggccacaccccaggcccagggatgggaccccacagtggtcacatcatcttgcagcagaacccaggtacagctcctggagcagatggtggtcccaagcacgggtgggaccagaaaggactctcacctgggctaactcagctgcagcctcagttccctcctcacacacgacgaggaacatggactggaagcctgcccagcaggccttctgctcgatgtgcgttgtgtggcttacgtccagggagggaagcagcctctgtgctgtcttctagataagcctgtattccccgggctgtctgccaatgtatccagttgtcccgtcagcctggaagctctgagggaaaaccttgggctgcttcctgagcacctgtatcccctgcagccagcccggggcctctgctaggagcagactgagcatggatatgggcctggcaccatctggcctctgcccaccttgctggccttgtcttgtgtctgccccttcgacattccatagcccagctcaatatctagtggttcctctagggtggcgagcactgtttggtctccagatgtcttcaggtcggagctcacagcgctctcagccaccccttcccagtgtagcaccgggcacatggtagatgcctattgatgagtgaaagctcctaacacactcagagagcaaggactccgcctcatcccacagcctgggaggagaggcagactgccaaggacctgctcagcatgctacagaagaaaccaaagtgcccacgggactgatcagtggagcttcctgccgagactggaggccttagggcagggtagacagtgtgtgtgcaggctggggactcacagttcggactgtgcccagacctactagcatagtgggtgggtgggaggatgcgggactgggggccgaccttgcctgaaattcatgtgggatctcagagcagccactgaattgctctgtagggggctaaatagtggcccccacagatacacacacccagacagagcctgtgagccagaccttatttggagaaaaggtctttgtagatgtaattaagcatctcaagatggcatcatctggattatgcggtgggctgtaagtcctgtgatgtgtctttATGGGAGTGCAGGTGGAAACCATCTCCCCAGGAGACGGGCGCACCTTCCCCAAGCGCGGCCAGACCTGCGTGGTGCACTACACCGGGATGCTTGAAGATGGAAAGAAAGTTGATTCCTCCCGGGACAGAAACAAGCCCTTTAAGTTTATGCTAGGCAAGCAGGAGGTGATCCGAGGCTGGGAAGAAGGGGTTGCCCAGATGAGTGTGGGTCAGAGAGCCAAACTGACTATATCTCCAGATTATGCCTATGGTGCCACTGGGCACCCAGGCATCATCCCACCACATGCCACTCTCGTCTTCGATGTGGAGCTTCTAAAACTG

AGAGAAAGGCAGAGGGAGATTTGACACACACAGGAGGGGCCACGTGGAGACAGAGGTGGAGATTGGAGAAATGTGGCCACAAGCCAGGGAACACCAGCAGCCACCAGAAGCCGGAAGACGTGAGGCAGGGTTCTTCCCAGAGCCTTCGCTGCTGAGTCTGGGAATTTGTGACCGAAGCCATAAGAAGTGGGTACACGCCCTGAGCCTCCCACACTTGCTCACCTGTCCTGAGATGAGAATCTCTACTCTGCAGCATATTTGGAGGATCACTGCGGGGGCCACAGAGGTGCTGTTCAGATGGCACTTCAGAAGACTCAGGAGACCCTGGGGCAGGAGCAGTTTGACTGACAGCCCAGAGGGCTGCCCTCTGATTCCACCTGAGGCCCTGCTTTTCCTGGCTGCAGGGGTTCCAGGGCCAGGCCATTTCCGCTGGCGCAGGACTCTGCTAGCAGCAACCTGCCTGAAGTCTTCCTTTGGCCTGGCTGAGAGTTTCTGAGACCTGCGCTGGAGCGGAGGTGCTTCCTTCCTTGCTTCCTTTCTTCCTCTCTCCCTTCTCCATCCAGCAGGCTGGACCTGCCTGGCATCTGTGAGCTCTCCCTACTTTCTCCTATACCCTAACCTTTGTCCTGCATGGGCGACTCCCCCAGTGAGTCTCTTGCAGCTTTTACCCCAGTGCCTGCTTCTTGGAGAATCCAAACTGATCCAGTTAGGGATGATAAAGTGTAGGGTAGGCGCTCGGTGACTGTTTTCTCTGAGGTTGTGACTCGTGTGAGGCAGAAGCAGTCCCCGTGAGCCCTCCTGGTATCTTGTGGAGTGGAGAACGCTTGGACCTGGAGCCAGGAGGCCCAGACATACATCCTGTCCGAGCTGCAGCTTCCTGTCTCTAAAATGAGCCGGCCAGCGCAGGTGGCCAGACATCACTGTTATTCTCCTTTGAGTCTTTAAATCTTGTTGTCTTTCTTGCAGACTCGGTGAGCTGTGAAAGGCTATAATAGGGGCTTTATTTTACACTTTGATACTATTTTTTGAACATTCATATTATTGTTAGATATTGATATTCATATGAAGGAGCAGGATGACTTGGGTCCTTCTTGGCAGTAGCATTGCCAGCTGATGGCCTTGGACAGTTACCTGCCCTCTCTAGGCCTCCCTTTCCTTGTCTATGAAATACATTATAGAATAGGATGTAGTGTGTGAGGATTTTTTGGAGGTTAAACGAGTGAATATATTTAAGGCGCTTTCACCAGTGCCTGGGATGTGCTCTGTAGTTTCTGTGTGTTAACTATAAGGTTGACTTTATGCTCATTCCCTCCTCTCCCACAAATGtcgccttggaaagacggaggcagcctggtggaggtgtatctcctagacaccagcatacagagtgaccaccgggaaatcgagggcagggtcatggtcaccgacttcgagaatgtgcccgaggaggacgggacccgcttccacagacaggtaagcacggccgtctgatgggagggctgcctctgcccatatccccatcctggaggtgggtggggactgccaccccagagcgttgcagctgtactcctgggttgcaccccccccagctgtcactgtcccctccctgccatcagttgtgggaagggcgttcatccatccagccacctgctgatttgttatagggtggagggggggtctttctcatgtggtccttgtgttcgtcgagcaggccagcaagtgtgacagtcatggcacccacctggcaggggtggtcagcggccgggatgccggcgtggccaagggtgccagcatgcgcagcctgcgcgtgctcaactgccaagggaagggcacggttagcggcaccctcataggtaagtgatggccccagacgctggtctctctccatctggacctggcctgggaggtggcttgggctgggcccagggagagctaatgtctcctaaccaagaatgctgtggcagcctctgccgcagagccagagaaccagagtgccaaggctggcagggttcccagtggccacgagtgcagatgaagaaacccaggccccaagagggtcatgcaggtagcccagggagttcagccttgaccctgggtcaatgacctttccacagttccacactgctcccatttaaaatccggtgatgtctttatgtcttttgttatgttatcttcaatgtggagggactcgaggtgatctaagcaaactttttctatcttctgcttgcatacctctgagaccaggggactcactcacttgcatgactgggccctgcaggtcacactggccaggcagatgtggtggaggaactggcagaggactttttctagactgtgactacatttagtccacccagcggcccccctatgaagtccagttgagaactaggactctgggggccggtggacagagaagag.

Example 2: β-catenin (CTNNB1)-dTAG

To further describe the targeting of endogenous proteins of interest fordegradation through the use of a dTAG as contemplated herein, thetargeting of an exemplary protein of interest, 3-catenin (CTNNB1), fordTAG insertion is illustrated.

β-catenin is encoded by the CTNNB1 gene. β-catenin regulates bothcell-cell adhesion and gene transcription as a downstream effector ofthe WNT signaling pathway. Under normal conditions, f-catenin functionand expression is mediated by phosphorylation and ubiquitin mediateddestruction via the βTrCP E3 ligase. Normally, β-catenin is regulatedupon binding to a repressive complex, which includes, axin, GSK33, andAPC. Upon WNT stimulation, axin is sequestered to frizzled receptors,thus releasing β-catenin from the destruction complex. The protein thentranslocates to the nucleus to bind TCF/LEF to activate transcriptionalprograms. Upon release of Wnt ligands, free beta-catenin isphosphorylated by GSK3 (and degraded through binding and ubiquitinationby βTrCRP E3 ligase.

The Wnt/β-catenin pathway is frequently mutated in human cancers, with1β-catenin mutations being observed in nearly 25% of hepatocellularcarcinoma. Recurrent mutations are found within the βTrCP binding site,conferring stability to the oncogenic transcriptional regulator. While abonafide oncology target, historical small molecule programs have failedas 1β-catenin is a relatively flat protein with few known ligands thatbind with high affinity. These data suggested β-catenin as an exemplarygene to target for conditional degradation.

To engineer the endogenous protein-dTAG hybrid protein, a homologousdonor construct is cloned that includes a left homology region (portionof intron 1), dTAG nucleic acid sequence (derived from the dTAGFKBP*—SEQ. ID. NO.: 2) cloned in frame with a short exon 1 of CTNNB1,intron 2, exon2, and a right homology region (portion of intron 3). ThedTAG nucleic acid sequence is cloned in frame with a 2X glycine linker.

To initiate homologous recombination, a CRISPR sgRNA is designed totarget the coding sequence β-catenin in exon 2. CAS9 expression inducesa double strand break which is repaired by homologous recombinationrepair using the donor construct as template. The end result is a genelocus with a dTAG nucleic acid sequence cloned in frame with exon 1 ofCTNNB1.

As derived, the resultant nucleic acid sequence including the in framedTAG nucleic acid insert results in the following genomic nucleic acidsequence, wherein lower case letters indicate intronic sequences of theCTNNB1 genomic sequence, capital, underlined sequences indicate thesgRNA target

(SEQ. ID. NO.: 49) (TACCACAGCTCCTTCTCTGAGTGG),ATG indicates the transcriptional start site of the CTNNB1 protein(βcatenin) or β-catenin (CTNBB1)-dTAG hybrid, capital letters indicatethe exon coding sequence of the β-catenin protein, and capital,italicized letters indicate the in frame insertion of the FKBP* deriveddTAG nucleic acid with a 2X glycine linker (GGGGGG) (SEQ. ID. NO.: 46).An illustration representing the exemplified HR strategy is provided forin FIG. 3.

CTNNB1 Genomic Locus (SEQ. ID. NO.: 50)aaataatttttgatggcactatatcagaaaacaacttgttaaagaaaatgtggagtttttaaaatcccactgtacctctgttatccaaaggggatctgtgaatttttctgtgaaaggttaaaaaaggagagacctttaggaattcagagagcagctgatttttgaatagtgttttcccctccctggcttttattattacaactctgtgattttcatcaccatcctgaatatctataattaatatttatactattaataaaaagacatttttggtaaggaggagttttcactgaagttcagcagtgatggagctgtggttgaggtgtctggaggagaccatgaggtctgcgtttcactaacctggtaaaagaggatatgggttttttttgtgggtgtaatagtgacatttaacaggtatcccagtgacttaggagtattaatcaagctaaatttaaatcctaatgacttttgattaactttttttagggtatttgaagtataccatacaactgttttgaaaatccagcgtggaca ATGGCTACTCAAGgtttgtgtcattaaatctttagttactgaattggggctctgcttcgttgccattaagccagtctggctgagatccccctgctttcctctctccctgcttacttgtcaggctaccttttgctccattttctgctcactcctcctaatggcttggtgaaatagcaaacaagccaccagcaggaatctagtctggatgactgcttctggagcctggatgcagtaccattcttccactgattcagtgagtaactgttaggtggttccctaagggattaggtatttcatcactgagctaaccctggctatcattctgcttttcttggctgtctttcagatttgactttatttctaaaaatatttcaatgggtcatatcacagattctttttttttaaattaaagtaacatttccaatctactaatgctaatactgtttcgtatttatagCTGATTTGATGGAGTTGGACATGGCCATGGAACCAGACAGAAAAGCGGCTGTTAGTCACTGGCAGCAACAGTCTTACCTGGACTCTGGAATCCATTCTGGTGCCACTACCACAGCTCCTTCTCTGAGTGGTAAAGGCAATCCTGAGGAAGAGGATGTGGATACCTCCCAAGTCCTGTATGAGTGGGAACAGGGATTTTCTCAGTCCTTCACTCAAGAACAAGTAGCTGgtaagagtattatattcattgccttactgaaagtcagaatgcagttttgagaactaaaaagttagtgtataatagtttaaataaaatgttgtggtgaagaaaagagagtaatagcaatgtcacttttaccatttaggatagcaaatacttaggtaaatgctgaactgtggatagtgagtgttgaattaaccttttccagATATTGATGGACAGTATGCAATGACTCGAGCTCAGAGGGTACGAGCTGCTATGTTCCCTGAGACATTAGATGAGGGCATGCAGATCCCATCTACACAGTTTGATGCTGCTCATCCCACTAATGTCCAGCGTTTGGCTGAACCATCACAGATGCTGAAACATGCAGTTGTAAACTTGATTAACTATCAAGATGATGCAGAACTTGCCACACGTGCAATCCCTGAACTGACA Resultant CTNNB1-dTAG Hybrid (SEQ. ID. NO.: 51)aaataatttttgatggcactatatcagaaaacaacttgttaaagaaaatgtggagtttttaaaatcccactgtacctctgttatccaaaggggatctgtgaatttttctgtgaaaggttaaaaaaggagagacctttaggaattcagagagcagctgatttttgaatagtgattcccctccctggcttttattattacaactctgtgattttcatcaccatcctgaatatctataattaatatttatactattaataaaaagacatttttggtaaggaggagttttcactgaagttcagcagtgatggagctgtggttgaggtgtctggaggagaccatgaggtctgcgtttcactaacctggtaaaagaggatatgggttttttttgtgggtgtaatagtgacatttaacaggtatcccagtgacttaggagtattaatcaagctaaatttaaatcctaatgacttttgattaactttttttagggtatttgaagtataccatacaactgttttgaaaatccagcgtggaca ATGGGAGTGCAGGTGGAAACCATCTCCCCAGGAGACGGGCGCACCTTCCCCAAGCGCGGCCAGACCTGCGTGGTGCACTACACCGGGATGCTTGAAGATGGAAAGAAAGTTGATTCCTCCCGGGACAGAAACAAGCCCTTTAAGTTTATGCTAGGCAAGCAGGAGGTGATCCGAGGCTGGGAAGAAGGGGTTGCCCAGATGAGTGTGGGTCAGAGAGCCAAACTGACTATATCTCCAGATTATGCCTATGGTGCCACTGGGCACCCAGGCATCATCCCACCACATGCCACTCTCGTCTTCGATGTGGAGCTTCTAAAACT

ATGGCTACTCAAGgtttgtgtcattaaatctttagttactgaattggggctctgcttcgttgccattaagccagtctggctgagatccccctgctttcctctctccctgcttacttgtcaggctaccttttgctccattttctgctcactcctcctaatggcttggtgaaatagcaaacaagccaccagcaggaatctagtctggatgactgcttctggagcctggatgcagtaccattcttccactgattcagtgagtaactgttaggtggttccctaagggattaggtatttcatcactgagctaaccctggctatcattctgcttttcttggctgtctttcagatttgactttatttctaaaaatatttcaatgggtcatatcacagattctttttttttaaattaaagtaacatttcaatctactaatgctaatactgtacgtatttatagcCTGATTTGATGGAGTTGGACATGGCCATGGAACCAGACAGAAAAGCGGCTGTTAGTCACTGGCAGCAACAGTCTTACCTGGACTCTGGAATCCATTCTGGTGCCACTACCACAGCTCCTTCTCTGAGTGGTAAAGGCAATCCTGAGGAAGAGGATGTGGATACCTCCCAAGTCCTGTATGAGTGGGAACAGGGATTTTCTCAGTCCTTCACTCAAGAACAAGTAGCTGgtaagagtattatttttcattgccttactgaaagtcagaatgcagttttgagaactaaaaagttagtgtataatagtttaaataaaatgttgtggtgaagaaaagagagtaatagcaatgtcacttttaccatttaggatagcaaatacttaggtaaatgctgaactgtggatagtgagtgttgaattaaccttttccagATATTGATGGACAGTATGCAATGACTCGAGCTCAGAGGGTACGAGCTGCTATGTTCCCTGAGACATTAGATGAGGGCATGCAGATCCCATCTACACAGTTTGATGCTGCTCATCCCACTAATGTCCAGCGTTTGGCTGAACCATCACAGATGCTGAAACATGCAGTTGTAAACTTGATTAACTATCAAGATGATGCAGAACTTGCCACACGTGCAATCCCTGAACTGACA

In any of the below examples either dFKBP13-o and dFKBP 13-p or dFKBP7-oand dFKBP7-p can be used.

Example 3

FIG. 4 illustrates an example to confirm selective degradation ofFKBP*-fused proteins with dFKBP7.

The dTAG is predicated on the selectivity of FKBP* specific ligands overendogenous, wild type FKBP. In 293T cells expressing wild type FKBP12 orFKBP*, dFKBP7 induces targeted degradation only in FKBP* expressingcells. An immunoblot of cells treated with heterobifunctional compoundsdescribed in the present invention was performed. 293FT cells (CRBN-WTor CRBN−/−) expressing either HA-tagged FKBP12WT or FKBP* were treatedwith indicated concentrations of dFKBP7 for 4 hours. CRBN-dependentdegradation of FKBP* and not FKBPWT confirms selective activity ofdFKBP7 for mutant FKBP*.

Example 4

FIGS. 5A-B illustrate an example of profiling of a panel of dFKBPheterobifunctional compounds to measure differential degradationactivity.

In an effort to identify potent and selective dFKPB heterobifunctionalcompounds, high throughput measurements of targeted FKBP* degradationwere measured by surrogate levels of luciferase. Here, FKBP* isexogenously expressed as a multicistronic transcript with two types ofluciferase: nano luciferase (NLuc) and firefly luciferase (FLuc) thatallow for cell normalized quantification of FKBP* protein levels.Degradation of FKBP* is measured as a signal ration (Nluc/Fluc) in wildtype (FIG. 4A) or CRBN −/− (FIG. 4B) 293FT cells treated with indicatedconcentrations of dFKBPs for 4 hours. A decrease in the signal ratioindicates FKBP* (Nluc) degradation and molecules that effectivelydegrade FKBP* in a cereblon dependent manner are observed (ex. dFKBP7).

Example 5

FIG. 6 illustrates an example of selective degradation of FKBP*-fusedproteins with dFKBP7 and dFKBP13, bifunctional molecules described inthe present invention.

In 293T cells expressing wild type FKBP12 or FKBP*, treatment withdFKBP7 and dFKBP13 induces targeted degradation only in FKBP* expressingcells. Isogenic 293FT cells (CRBN-WT or CRBN−/−) were engineered toexpressed either FKBP12WT or FKBP*. Cells were treated with 100 nM ofeither dFKBP7 or dFKBP13 for 4 hours before lysates were prepared forwestern immunoblot analysis. CRBN-dependent degradation of FKBP* and notFKBP12WT or endogenous FKBP12 confirms selectivity of dFKBP7 and dFKBP13for mutant FKBP*.

Example 6

FIG. 7 illustrates and example of dose-dependent degradation ofHA-tagged FKBP12* with a bifunctional molecule dFKBP13.

In an effort to define the optimal concentration of dFKB13heterobifunctional compound to induce degradation of FKBP*, degradationwas measured upon treatment with increasing concentrations of dFKBP13.Isogenic 293FT cells (CRBN-WT or CRBN−/−) were engineered to expressedHA-tagged FKBP*. Cells were treated with the indicated dose of dFKBP13for 4 hours before lysates were prepared for western immunoblotanalysis. These data confirm dose- and CRBN-dependent degradation ofHA-tagged FKBP* by dFKBP 13.

Example 7

FIG. 8 illustrates the kinetic control of dFKBP13-dependent degradationof HA-tagged FKBP*.

To evaluate the kinetic control of targeted degradation FKBP*, dFKBP13was administered by increased duration. 293FT cells (CRBN-WT) wereengineered to express HA-tagged FKBP*. Cells were treated with 100 nMdFKBP13 for the indicated times. Cells were harvested and proteinlysates immunoblotted to measure the kinetics of HA-tagged FKBP*degradation induced by dFKBP13.

Example 8

FIG. 9 illustrates and example to confirm CRBN- and proteasome-dependentdegradation of FKBP* by the bifunctional molecule dFKBP13.

293FT cells (CRBN-WT) were engineered to express FKBP*. Cells werepretreated with 1 uM Carfilzomib (proteasome inhibitor), 0.5 uM MLN4924(neddylation inhibitor), and 10 uM Lenalidomide (CRBN binding ligand)for two hours prior to a 4 hour treatment with dFKBP13. Lysates wereprepared and western immunoblot analysis performed. Degradation ofHA-tagged FKBP* by dFKBP13 was rescued by the proteasome inhibitorCarfilzomib, establishing a requirement for proteasome function.Pre-treatment with the NAE1 inhibitor MLN4924 rescued HA-tagged FKBP*establishing dependence on CRL activity, as expected for cullin-basedubiquitin ligases that require neddylation for processive E3 ligaseactivity. Pre-treatment with excess Lenalidomide abolisheddFKBP13-dependent FKBP* degradation, confirming the requirement of CRBNengagement for degradation.

Example 9

FIGS. 10A-B confirms targeted degradation of proteins of interest whenfused to dTAG.

To test the general utility of the dTAG technology across severalprotein types, the indicated proteins fused to the dTAG in MV4;11leukemia cells were expressed. Upon treatment with the indicated dFKBPbifunctional molecules (dFKBP7 and dFKBP13), targeted proteindegradation was observed as measured by western blot. Cells were treatedfor 16 hours with indicated concentrations of FKBP* selectiveheterobifunctional compounds and degradation was observed with nanomolarconcentrations.

Example 10

FIG. 11 illustrates an example confirming degradation of N-terminaldTAG-KRAS.

In N-terminal dTAG-KRAS, dFKBP7 treatment resulted in potent degradationas well as a downstream decrease in p-AKT signal suggesting thebiological relevance of overexpressed endogenous protein-dTAG hybridproteins. Cells were treated with 500 nM dFKBP7 for the indicated time.Cells were harvested and immunoblotted to measure degradation ofFKBP*-KRAS and downstream surrogates of KRAS signaling (e.g. pMEK andpAKT). Overexpression of dTAG KRAS resulted in the activation of therelevant downstream signaling pathways as an observed increase in p-AKTsignal as measured by western blot.

Example 11

FIG. 12 illustrates the profiling of dFKBP heterobifunctional compoundsto induce degradation of dTAG-KRAS.

In an effort to identify the best performing dFKBP molecule, dTAG-KRASdegradation was profiled across a series of dFKBP molecules. Westernblotting of NIH3T3 cells expressing dTAG-KRASG12V were treated with 1 μMof the indicated dFKBP heterobifunctional compounds for 24 hours. Cellswere harvested and immunoblotted to measure degradation of FKBP*-KRASand downstream surrogates of KRAS signaling (e.g. pMEK and pAKT). Thedata suggest that dFKBP9, dFKBP 12, and dFKBP13 induce potentdegradation of FKBP*-KRAS and inhibition of downstream signaling.

Example 12

FIG. 13 illustrates an example confirming targeted degradation ofdTAG-KRAS with dFKBP13.

The dFKBP13 heterobifunctional compound potently degrades dTAG-KRAS atnanomolar concentrations. Western blotting of NIH3T3 cells expressingFKBP* fused to the N-terminus of KRAS treated with the indicatedconcentrations of dFKBP13 for 24 hours. Cells were harvested andimmunoblotted to measure degradation of FKBP*-KRAS and downstreamsurrogates of KRAS signaling (e.g. pMEK and pAKT). The data suggest thatdFKBP13 induces potent degradation of FKBP*-KRAS and inhibits downstreamsignaling potently with an IC50>100 nM.

Example 13

FIG. 14 illustrates an example of the kinetic control of targeteddegradation of dTAG-KRAS with dFKBP13.

To evaluate the kinetic control of targeted degradation of dTAG-KRAS,dFKBP13 was administered by increased duration. Western blotting ofNIH3T3 cells expressing FKBP* fused to the N-terminus of KRAS treatedwith 1 μM dFKBP13 for the indicated time. Cells were harvested andimmunoblotted to measure degradation of FKBP*-KRAS and downstreamsurrogates of KRAS signaling (e.g. pMEK and pAKT). The data suggest thatdFKBP13 induces potent degradation of FKBP*-KRAS and inhibition ofdownstream signaling as early as 1 hour post treatment.

Example 14

FIG. 15 illustrates and example to confirm CRBN- andproteasome-dependent degradation of dTAG-KRASG12V by theheterobifunctional compound dFKBP 13.

NIH3T3 cells (CRBN-WT) were engineered to express dTAG-KRASG12V. Cellswere pretreated with 1 uM Carfilzomib (proteasome inhibitor), 0.5 uMMLN4924 (neddylation inhibitor), and 10 uM Lenalidomide (CRBN bindingligand) for two hours prior to a 4 hour treatment with dFKBP13. Lysateswere prepared and western immunoblot analysis performed. Degradation ofdTAG-KRASG12V by dFKBP13 was rescued by the proteasome inhibitorCarfilzomib, establishing a requirement for proteasome function.Pre-treatment with the NAE1 inhibitor MLN4924 rescued dTAG-KRASG12Vexpression establishing dependence on CRL activity, as expected forcullin-based ubiquitin ligases that require neddylation for processiveE3 ligase activity. Pre-treatment with excess Lenalidomide abolisheddFKBP13-dependent dTAG-KRASG12V degradation, confirming the requirementof CRBN engagement for degradation.

Example 15

FIG. 16 illustrates an example confirming targeted degradation ofoncogenic dTAG-KRAS alleles with dFKBP13.

The dFKBP13 heterobifunctional compound potently degrades dTAG-KRASmutant alleles. NIH3T3 cells were engineered to express KRAS alleleseither WT or mutant forms of amino acid glycine 12 (G12C, G12D, andG12V). Western blotting of NIH3T3 cells expressing dTAG fused to theN-terminus of KRAS alleles were treated with 1 uM of dFKBP13 for 24hours. Cells were harvested and immunoblotted to measure degradation ofdTAG-KRAS and downstream surrogates of KRAS signaling (e.g. pMEK andpAKT). The data suggest that dFKBP13 induces potent degradation of WTand mutant KRAS alleles and potently inhibits downstream signaling.

Example 16

FIG. 17 illustrates an example confirming targeted degradation ofoncogenic dTAG-KRAS alleles with dFKBP13.

The dFKBP13 heterobifunctional compound potently degrades dTAG-KRASmutant alleles. NIH3T3 cells were engineered to express either WT ormutant KRAS alleles (G13D, Q61L, and Q61R). Western blotting of NIH3T3cells expressing dTAG fused to the N-terminus of KRAS alleles weretreated with 1 uM of dFKBP13 for 24 hours. Cells were harvested andimmunoblotted to measure degradation of dTAG-KRAS and downstreamsurrogates of KRAS signaling (e.g. pMEK and pAKT). The data suggest thatdFKBP13 induces potent degradation of WT and mutant KRAS alleles andpotently inhibits downstream signaling.

Example 17

FIGS. 18A-D illustrates an experiment performed to confirm phenotypicalchanges induced upon degradation of dTAG-KRAS.

Morphological changes were observed in NIH3T3 cells upon overexpressionof dTAG-KRAS as shown by phase contrast imaging. Upon treatment withdFKBP13 for 24 hours, cells morphologically revert back to the wild type(DMSO control) state.

Example 18

FIGS. 19A-D illustrates the phenotypic consequence of dTAG-KRASdegradation on the viability of NIH3T3 cells.

The ATPlite 1-step luminescence assay measures cell proliferation andcytotoxicity in cells based on the production of light caused by thereaction of ATP with added luciferase and D-luciferin. A decrease insignal indicates a reduction in cell number. To evaluate the effect ofdFKBP13 on proliferation in NIH3T3 cells expressing dTAG-KRAS, viabilitywas assessed by surrogate measurements of ATP levels. Cells were treatedwith the indicated concentrations of dFKBPs for 72 hours and cellviability was measured using an ATPlite assay.

Example 19

FIG. 20 illustrates the phenotypic consequence of dTAG-KRAS degradationon the cell cycle profile of NIH3T3 cells.

NIH3T3 cells were engineered to express dTAG-KRASG12V. NIH3T3 cellsexpressing dTAG-KRASG12V were treated with dFKBP7 and dFKBP13 for 48hours to induce targeted dTAG-KRASG12V degradation. Fixed cells werestained with propidium iodide and cell cycle analysis was performed.Treatment with both dFKBP7 and dFKBP13 resulted in diminished S-phaseentry, in agreement with the biological role of endogenous KRASG12V indriving S-phase entry. These data are consistent with the observedeffect on dTAG-KRASG12V degradation on cell viability.

Example 20: Delivery of CRISPR-CAS9 and Homologous Donor Vectors to theLiver

Targeted gene therapy can be accomplished using both viral and non-viralapproaches such as adeno-associated or lentivirus, or lipid-basedformulations. For example, a single bicistronic vector system is used todeliver sgRNA targeting either PCKS9 or CTNNB1 with CAS9 being expressedfrom a neighboring promoter. Both the CRISPR vector and donor homologyplasmid are encapsulated in Poly (beta-amino esters) (PBAEs) cationicpolymers that provide the added specificity of cancer cell targeting vs.normal hepatocytes. PBAE nanoparticles are also biodegradable, ordegrade by hydrolysis, thus releasing plasmid DNAs in the cytoplasm oftumor cells upon internalization. PBAE-encapsulated plasmid DNAs will bedelivery locally via intrahepatic artery administration and systemicallyvia intravenous injection. Upon successful recombination, and followingadministration of a heterobifunctional compound, local core biopsieswould be taken to confirm degradation of either the PCKS9 gene productor the CTNNB1 gene product.

This specification has been described with reference to embodiments ofthe invention. However, one of ordinary skill in the art appreciatesthat various modifications and changes can be made without departingfrom the scope of the invention as set forth in the claims below.Accordingly, the specification is to be regarded in an illustrativerather than a restrictive sense, and all such modifications are intendedto be included within the scope of invention.

1. A transformed cell comprising: a genomically integrated nucleic acidsequence encoding a heterobifunctional compound targeting proteincapable of being bound by a heterobifunctional compound; wherein thedTAG comprises an amino acid sequence derived from EGFR, BCR-ABL, ALK,JAK2, BRAF, Src, LRRK2, PDGFRα, or RET; wherein the nucleic acidsequence encoding the dTAG is integrated genomically in-frame in a 5′ or3′ orientation with a nucleic acid sequence of a gene encoding anendogenous protein; wherein expression of the gene encoding anendogenous protein produces an endogenous protein-dTAG hybrid protein;wherein the heterobifunctional compound is capable of binding to a) theendogenous protein-dTAG hybrid protein through the dTAG and b) aubiquitin ligase in a manner that brings the endogenous protein-dTAGhybrid protein into proximity of the ubiquitin ligase; wherein theendogenous protein-dTAG hybrid protein is ubiquitinated and thendegraded by a proteasome.
 2. The transformed cell of claim 1, whereinthe cell is a human cell.
 3. The transformed cell of claim 2, whereinthe human cell is a liver cell.
 4. The transformed cell of claim 1,wherein the heterobifunctional compound targeting protein comprises anamino acid sequence from a non-endogenous protein.
 5. The transformedcell of claim 1, wherein the heterobifunctional compound targetingprotein comprises an amino acid sequence selected from SEQ. ID. NO.:53-63.
 6. (canceled)
 7. (canceled)
 8. (canceled)
 9. (canceled) 10.(canceled)
 11. (canceled)
 12. (canceled)
 13. (canceled)
 14. (canceled)15. (canceled)
 16. (canceled)
 17. (canceled)
 18. (canceled) 19.(canceled)
 20. (canceled)
 21. (canceled)
 22. (canceled)
 23. (canceled)24. (canceled)
 25. (canceled)
 26. The transformed cell of claim 1,wherein the nucleic acid sequence encoding the heterobifunctionalcompound targeting protein is inserted in frame with a gene encoding anendogenous protein associated with a disease that is a result of a gainof function mutation, amplification or increased expression, amonogenetic disease, a proteopathy, or a combination thereof.
 27. Thetransformed cell of claim 1, further comprising a nucleic acid sequenceencoding a CRISPR RNA-guided endonuclease.
 28. The transformed cell ofclaim 27, wherein the CRISPR RNA-guided endonuclease is selected fromthe group consisting of Cas1, Cas IB, Cas2, Cas3, Cas4, Cas5, Cas6,Cas7, Cas8, Cas9, Cas10, Csy1, Csy2, Csy3, Cse1, Cse2, Csc1, Csc2, Csa5,Csn2, Csm2, Csm3, Csm4, Csm5, Csm6, Cmr1, Cmr3, Cmr4, Cmr5, Cmr6, Csb1,Csb2, Csb3, Csx17, Csx14, Csx10, Csx16, CsaX, Csx3, Csx1, Csx15, Csf1,Csf2, Csf3, Csf4, and Cpf1.
 29. The transformed cell of claim 28,wherein the nucleic acid encodes a Cas9 endonuclease comprised of anamino acid sequence of SEQ. ID. NO.:
 52. 30. The transformed cell ofclaim 1, wherein the heterobifunctional compound targeting protein doesnot substantially interfere with the function of the endogenouslyexpressed protein.
 31. A method of modulating gene expression in asubject comprising: administering to the subject an effective amount ofa heterobifunctional compound; wherein the subject has one or moretransformed cells that have been transformed with a nucleic acidsequence encoding a heterobifunctional compound targeting protein(dTAG); wherein the dTAG comprises an amino acid sequence derived fromEGFR, BCR-ABL, ALK, JAK2, BRAF, Src, LRRK2, PDGFRα, or RET; wherein thenucleic acid sequence encoding the dTAG is integrated genomicallyin-frame in a 5′ or 3′ orientation with a nucleic acid sequence of anendogenous protein associated with a disease; wherein the insertion ofthe nucleic acid sequence encoding the dTAG into the genomic sequenceresults in an endogenous protein-dTAG hybrid protein upon expression;wherein the heterobifunctional compound binds to a) the endogenousprotein-dTAG hybrid protein through the dTAG and b) a ubiquitin ligasein a manner that brings the endogenous protein-dTAG hybrid protein intoproximity of the ubiquitin ligase, wherein the endogenous protein-dTAGhybrid protein is ubiquitinated and then degraded by a proteasome. 32.The method of claim 31, wherein the cell is a human cell.
 33. The methodof claim 32, wherein the human cell is a liver cell.
 34. The method ofclaim 31, wherein the heterobifunctional compound targeting proteincomprises an amino acid sequence from a non-endogenous protein.
 35. Themethod of claim 31, wherein the heterobifunctional compound targetingprotein comprises an amino acid sequence selected from SEQ. ID. NO.:53-63.
 36. (canceled)
 37. (canceled)
 38. (canceled)
 39. (canceled) 40.(canceled)
 41. (canceled)
 42. (canceled)
 43. (canceled)
 44. (canceled)45. (canceled)
 46. (canceled)
 47. (canceled)
 48. (canceled) 49.(canceled)
 50. (canceled)
 51. (canceled)
 52. (canceled)
 53. (canceled)54. (canceled)
 55. (canceled)
 56. The method of claim 31, wherein thenucleic acid sequence encoding the heterobifunctional compound targetingprotein is inserted in frame with a gene encoding an endogenous proteinassociated with a disease that is a result of a gain of functionmutation, amplification or increased expression, a monogenetic disease,a proteopathy, or a combination thereof.
 57. The method of claim 31,further comprising a nucleic acid sequence encoding a CRISPR RNA-guidedendonuclease.
 58. The method of claim 57, wherein the CRISPR RNA-guidedendonuclease is selected from the group consisting of Cas1, Cas IB,Cas2, Cas3, Cas4, Cas5, Cas6, Cas7, Cas8, Cas9, Cas10, Csy1, Csy2, Csy3,Cse1, Cse2, Csc1, Csc2, Csa5, Csn2, Csm2, Csm3, Csm4, Csm5, Csm6, Cmr1,Cmr3, Cmr4, Cmr5, Cmr6, Csb1, Csb2, Csb3, Csx17, Csx14, Csx1O, Csx16,CsaX, Csx3, Csx1, Csx15, Csf1, Csf2, Csf3, Csf4, and Cpf1.
 59. Themethod of claim 58, wherein the nucleic acid encodes a Cas9 endonucleasecomprised of an amino acid sequence of SEQ. ID. NO.:
 52. 60. The methodof claim 31, wherein the heterobifunctional compound targeting proteindoes not substantially interfere with the function of the endogenouslyexpressed protein.
 61. A method of reducing gene overexpression in asubject comprising: administering to the subject an effective amount ofa heterobifunctional compound; wherein the subject has one or moretransformed cells that have been transformed with a nucleic acidsequence encoding a heterobifunctional compound targeting protein(dTAG); wherein the dTAG comprises an amino acid sequence derived fromEGFR, BCR-ABL, ALK, JAK2, BRAF, Src, LRRK2, PDGFRα, or RET; wherein thenucleic acid sequence encoding the dTAG is integrated genomicallyin-frame in a 5′ or 3′ orientation with a nucleic acid sequence of anendogenous protein associated with a disease due to overexpression of aprotein; wherein the insertion of the nucleic acid sequence encoding thedTAG into the genomic sequence results in an endogenous protein-dTAGhybrid protein upon expression; wherein the heterobifunctional compoundbinds to a) the endogenous protein-dTAG hybrid protein through the dTAGand b) a ubiquitin ligase in a manner that brings the endogenousprotein-dTAG hybrid protein into proximity of the ubiquitin ligase,wherein the endogenous protein-dTAG hybrid protein is ubiquitinated andthen degraded by a proteasome.
 62. The method of claim 61, wherein thecell is a human cell.
 63. The method of claim 62, wherein the human cellis a liver cell.
 64. The method of claim 61, wherein theheterobifunctional compound targeting protein comprises an amino acidsequence from a non-endogenous protein.
 65. The method of claim 61,wherein the heterobifunctional compound targeting protein comprises anamino acid sequence selected from SEQ. ID. NO.: 53-63.
 66. (canceled)67. (canceled)
 68. (canceled)
 69. (canceled)
 70. (canceled) 71.(canceled)
 72. (canceled)
 73. (canceled)
 74. (canceled)
 75. (canceled)76. (canceled)
 77. (canceled)
 78. (canceled)
 79. (canceled) 80.(canceled)
 81. (canceled)
 82. (canceled)
 83. (canceled)
 84. (canceled)85. (canceled)
 86. The method of claim 61, wherein the nucleic acidsequence encoding the heterobifunctional compound targeting protein isinserted in frame with a gene encoding an endogenous protein associatedwith a disease that is a result of a gain of function mutation,amplification or increased expression, a monogenetic disease, aproteopathy, or a combination thereof.
 87. The method of claim 61,further comprising a nucleic acid sequence encoding a CRISPR RNA-guidedendonuclease.
 88. The method of claim 87, wherein the CRISPR RNA-guidedendonuclease is selected from Cas1, Cas IB, Cas2, Cas3, Cas4, Cas5,Cas6, Cas7, Cas8, Cas9, Cas10, Csy1, Csy2, Csy3, Cse1, Cse2, Csc1, Csc2,Csa5, Csn2, Csm2, Csm3, Csm4, Csm5, Csm6, Cmr1, Cmr3, Cmr4, Cmr5, Cmr6,Csb1, Csb2, Csb3, Csx17, Csx14, Csx10, Csx16, CsaX, Csx3, Csx1, Csx15,Csf1, Csf2, Csf3, Csf4, and Cpf1.
 89. The method of claim 88, whereinthe nucleic acid encodes a Cas9 endonuclease comprised of an amino acidsequence of SEQ. ID. NO.:
 52. 90. The method of claim 61, wherein theheterobifunctional compound targeting protein does not substantiallyinterfere with the function of the endogenously expressed protein.